Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,187,980	T→C	9.2%	L162S (TTG→TCG)	PP_4567 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,187,980	0	T	C	9.2%	173.4 / 7.2	65	L162S (TTG→TCG)	PP_4567	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/32);  new base C (2/4);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

TTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAG  >  NC_002947/5187832‑5188125                                                                                                                                                     |                                                                                                                                                  ttGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGtt                                                                                                                                                   <  6:202097/149‑1 (MQ=255)    cTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTgc                                                                                                                                                 <  4:367462/148‑1 (MQ=255)        aTTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTCGcgccac                                                                                                                                            >  8:21442/1‑149 (MQ=255)         tttCGGCTGCGCCTGTGAAACTGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgccacg                                                                                                                                           <  3:189757/149‑1 (MQ=255)               cTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGAGCCACGCCATcc                                                                                                                                     >  7:127378/1‑149 (MQ=255)                  cgcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGATGGCGTGGCGCAACACGCCTTcct                                                                                                                                    <  4:161782/147‑2 (MQ=255)                  cgcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATccc                                                                                                                                    <  1:124387/147‑1 (MQ=255)                    ccTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGAGCCACGCCATCCCCGaa                                                                                                                                <  8:127378/149‑1 (MQ=255)                    ccTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGACGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGaa                                                                                                                                <  8:392585/149‑1 (MQ=255)                        tgAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAagt                                                                                                                              <  6:153426/147‑2 (MQ=38)                        tgAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATccc                                                                                                                                    >  5:144025/1‑141 (MQ=255)                        tgAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATccc                                                                                                                                    <  6:144025/141‑1 (MQ=255)                          aaaCCGAACAAGCAGTGAAGATGTTGGCGGGCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTCCTACCTTCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGGGTTGCGCCACGCCATCCCCGAAGAGTTc                                                                                                                          <  5:341783/149‑1 (MQ=255)                           aaCCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCAGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTc                                                                                                                          <  6:62949/148‑1 (MQ=255)                                      cAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCTTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTa                                                                                                              <  4:170740/149‑1 (MQ=255)                                               tGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGc                                                                                                     <  8:130135/149‑1 (MQ=255)                                                   ggCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAg                                                                                                  <  7:224187/148‑1 (MQ=255)                                                            ggTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCgcggcg                                                                                        <  2:90651/149‑1 (MQ=255)                                                             gTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGTAGGCGTGTTGCGCCACGCCATCCCGGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGcggcgg                                                                                       <  5:237544/149‑1 (MQ=255)                                                              tCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAgg                                                                                                                   >  4:206501/1‑120 (MQ=255)                                                              tCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAgg                                                                                                                   <  3:206501/120‑1 (MQ=255)                                                                ccgccgCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGATGGCGTGGCGCAACACGCCTTCCTCGaa                                                                                                                                >  2:37763/1‑105 (MQ=37)                                                                ccgccgCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCt                                                                                    >  3:3871/1‑149 (MQ=255)                                                                ccgccgCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTCCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCt                                                                                    >  3:297911/1‑149 (MQ=255)                                                                ccgccgCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGACGATGGCGTGGCGCAACACGCCTTCCTCGaa                                                                                                                                <  1:37763/105‑1 (MQ=37)                                                                 cgccgcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCtt                                                                                                         >  3:38270/1‑127 (MQ=255)                                                                 cgccgcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCtt                                                                                                         <  4:38270/127‑1 (MQ=255)                                                                  gccgcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTg                                                                                  <  3:363622/149‑1 (MQ=255)                                                                   ccgcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGc                                                                                 <  4:3871/149‑1 (MQ=255)                                                                            tcgctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACg                                                                         >  2:131035/1‑148 (MQ=255)                                                                             cgctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGaa                                                                       >  3:300680/1‑149 (MQ=255)                                                                              gctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCAGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAc                                                                      <  5:329364/149‑1 (MQ=255)                                                                                  gATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAacac                                                                          <  7:78585/141‑1 (MQ=255)                                                                                  gATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAacac                                                                          >  8:78585/1‑141 (MQ=255)                                                                                  gATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTCGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcag                                                                    <  7:21442/147‑1 (MQ=255)                                                                                       ccTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcagcag                                                                 >  6:393924/1‑145 (MQ=255)                                                                                       ccTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcagcag                                                                 <  5:393924/145‑1 (MQ=255)                                                                                       ccTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcag                                                                    >  8:151917/1‑142 (MQ=255)                                                                                       ccTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcag                                                                    <  7:151917/142‑1 (MQ=255)                                                                                       ccTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGccc                                                              >  5:306070/1‑148 (MQ=255)                                                                                          gCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTAc                                                           >  7:179006/1‑148 (MQ=255)                                                                                                   ccTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGc                                                 >  8:108708/1‑149 (MQ=255)                                                                                                      gCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGccc                                               >  4:314475/1‑148 (MQ=255)                                                                                                       cTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGcccc                                              <  7:108708/148‑1 (MQ=255)                                                                                                          aCCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCAt                                            <  7:343952/147‑1 (MQ=255)                                                                                                            ccAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAacac                                                                          <  3:268631/115‑1 (MQ=255)                                                                                                            ccAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAacac                                                                          >  4:268631/1‑115 (MQ=255)                                                                                                            ccAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGcc                                         >  1:303185/1‑148 (MQ=255)                                                                                                             cAGCGCGAAGCCGGCCTGCT‑GACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcag                                      >  4:22025/1‑149 (MQ=255)                                                                                                                          gCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCgg                          <  4:300680/149‑1 (MQ=255)                                                                                                                            ctgctgGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGTGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTg                        >  7:73072/1‑149 (MQ=255)                                                                                                                             tgctgGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGc                       >  5:393304/1‑149 (MQ=255)                                                                                                                                  gACTTCGAGGAAGGCGTGTTGCGCCATGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGc                  >  3:335818/1‑149 (MQ=255)                                                                                                                                   aCTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGTGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCg                 <  8:73072/149‑1 (MQ=255)                                                                                                                                   aCTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCg                 <  8:179006/149‑1 (MQ=255)                                                                                                                                     ttCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGt               >  6:46546/1‑149 (MQ=255)                                                                                                                                     ttCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGt               >  3:139183/1‑149 (MQ=255)                                                                                                                                     ttCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGt               >  5:215792/1‑149 (MQ=255)                                                                                                                                        gAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAc            >  6:282829/1‑149 (MQ=255)                                                                                                                                        gAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAc            <  3:314475/149‑1 (MQ=255)                                                                                                                                           gAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTa                                                            <  5:155892/98‑1 (MQ=255)                                                                                                                                           gAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGCCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTa                                                            >  6:155892/1‑98 (MQ=255)                                                                                                                                            aaGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGaa        <  5:282829/149‑1 (MQ=255)                                                                                                                                              ggCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCg          >  5:22751/1‑145 (MQ=255)                                                                                                                                               gCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGAt     <  4:139183/149‑1 (MQ=255)                                                                                                                                                  tgtTCCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAg  <  4:297911/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TTGCTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCTAGGGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATCAG  >  NC_002947/5187832‑5188125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑