| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,244,235 | G→C | 5.7% | P83A (CCG→GCG) | hmgA ← | homogentisate 1,2‑dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,244,235 | 0 | G | C | 5.7% | 291.4 / 6.3 | 87 | P83A (CCG→GCG) | hmgA | homogentisate 1,2‑dioxygenase |
| Reads supporting (aligned to +/- strand): ref base G (42/40); new base C (3/2); total (45/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01 | |||||||||||
TAGATGCTCACGCCAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGCGGGGAG > NC_002947/5244087‑5244383 | tagatgCTCACGCCAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCgg > 8:383460/1‑149 (MQ=255) gatgCTCACGCCAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGc < 7:303831/148‑1 (MQ=255) tCACGCCAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCTGTGGCCCGCCGAGCGGCTGgcgc > 4:225085/1‑149 (MQ=255) aCGCCAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGgcgcg < 7:107826/148‑1 (MQ=255) gCCAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGgcgcgc > 1:32522/1‑147 (MQ=255) cAGCCGGTTTTTCCGCTCGGGCTTTTGCAGCCATGGGCAGCCAACCTTCGATGAAAGCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGc < 6:269569/137‑1 (MQ=255) cAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAgg < 4:112420/149‑1 (MQ=255) cAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAgg > 2:271236/1‑149 (MQ=255) cAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAgg < 5:107094/149‑1 (MQ=255) cAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGAAGCCAACCTTCGATGAAATCGGTCGGTTAAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCTGTTGGGG‑TTGATGCAACCCAGTGGCCCGCCGAGCGGc > 5:269569/1‑137 (MQ=255) ccGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGgcgc > 4:363289/1‑149 (MQ=255) cGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCt > 8:371961/1‑149 (MQ=255) ggTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGCCTGGCGCGCCAGCCGCTc < 6:371100/149‑1 (MQ=255) tttttCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGCCTGGCGCGCCAg < 1:59222/141‑1 (MQ=255) tttttCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGCCTGGCGCGCCAg > 2:59222/1‑141 (MQ=255) tttCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGaa < 3:238431/148‑1 (MQ=255) tttCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGaa < 7:371961/148‑1 (MQ=255) cGCTCAGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTg > 3:99785/1‑149 (MQ=255) gCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGTCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTgg > 8:15666/1‑149 (MQ=255) gCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTgg < 7:383460/149‑1 (MQ=255) gCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTgg < 6:138734/149‑1 (MQ=255) gCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTgg > 3:69003/1‑149 (MQ=255) cGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTg > 4:10658/1‑148 (MQ=255) gCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGc > 6:146362/1‑147 (MQ=255) ggTTGGCTGCCCATGGCTGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAg > 4:93143/2‑149 (MQ=255) ggCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCACgg > 6:113061/1‑146 (MQ=255) cATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGc < 7:103038/149‑1 (MQ=255) aTGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGc > 6:14944/1‑148 (MQ=255) ggCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGaa < 5:141175/148‑1 (MQ=255) aGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCg < 2:32522/149‑1 (MQ=255) aGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCg > 1:154123/1‑149 (MQ=255) ccAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCgg < 3:93143/148‑1 (MQ=255) aaCCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGt < 1:153135/123‑1 (MQ=255) aaCCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGt > 2:153135/1‑123 (MQ=255) aaCCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTa > 6:42676/1‑148 (MQ=255) gATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCg < 5:362159/138‑1 (MQ=255) gATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCg > 6:362159/1‑138 (MQ=255) aTGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAgg > 3:276699/1‑148 (MQ=255) tGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGt < 2:387224/148‑1 (MQ=255) aTCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGgc < 5:42676/149‑1 (MQ=255) gtcggtTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAgg > 3:301992/1‑139 (MQ=255) gtcggtTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAgg < 4:301992/139‑1 (MQ=255) gtcggtTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAg > 3:151636/1‑149 (MQ=255) gtTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCg > 7:26860/1‑149 (MQ=255) gtTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCg > 3:340060/1‑149 (MQ=255) gtTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCg > 6:61148/1‑149 (MQ=255) ttCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGc > 3:195003/1‑149 (MQ=255) agcagGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGc < 4:151636/149‑1 (MQ=255) agcagGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGc > 5:252365/1‑149 (MQ=255) gcagGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAg < 8:368018/80‑1 (MQ=255) gcagGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAg > 7:368018/1‑80 (MQ=255) cagGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCgg < 5:14944/149‑1 (MQ=255) cagGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCgg < 5:146362/149‑1 (MQ=255) cagGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCgg > 2:36372/1‑149 (MQ=255) ggAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGc < 8:377624/149‑1 (MQ=255) gAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAgg > 6:257172/1‑106 (MQ=255) gAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAgg < 5:257172/106‑1 (MQ=255) tCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATg < 4:69003/149‑1 (MQ=255) tCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATg < 1:271236/149‑1 (MQ=255) ggCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCGAGGTACGGCGCAGCTCGCTGCGGGCCATgg < 1:36372/148‑1 (MQ=255) tGCGGGCTCCAGCGCAGGCGGTTGGGGCTTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTg > 1:225821/1‑148 (MQ=255) ggCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGcc < 4:99785/149‑1 (MQ=255) cTCCTGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGt < 7:15666/149‑1 (MQ=255) tCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTg > 5:145185/1‑149 (MQ=255) cAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGcc > 7:99572/1‑149 (MQ=255) cAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGc < 2:154123/148‑1 (MQ=255) cAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGa > 6:243015/1‑147 (MQ=255) gCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGAcagca < 3:10658/149‑1 (MQ=255) gggg‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCg < 2:359380/148‑1 (MQ=255) ggg‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGc < 5:243015/149‑1 (MQ=255) g‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCa > 5:338690/1‑148 (MQ=255) gATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATa > 6:118776/1‑147 (MQ=255) ccACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGcc < 3:225085/149‑1 (MQ=255) aCCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCa > 2:281803/1‑148 (MQ=255) aCCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCa > 3:52221/1‑148 (MQ=255) gTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACggg < 6:252365/149‑1 (MQ=255) gCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGc > 4:161769/1‑138 (MQ=255) gCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGc < 3:161769/138‑1 (MQ=255) cccACCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCt > 1:144994/1‑149 (MQ=255) cttcgAGCGCCTGGCGCGCCAGCCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTc > 7:316691/4‑149 (MQ=255) cttcgAGCGCCTGGCGCGCCAGCCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTc > 3:274773/4‑149 (MQ=255) cgAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGc < 5:61148/149‑1 (MQ=255) cgAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGc > 4:39399/1‑149 (MQ=255) aGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGCgg < 3:363289/149‑1 (MQ=255) aGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGCg < 5:239567/148‑1 (MQ=255) aGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGCg > 6:239567/1‑148 (MQ=255) cGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGCgggg > 3:111563/1‑149 (MQ=255) cGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGCggg < 8:99572/148‑1 (MQ=255) ggCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGCGGGGa < 8:26860/149‑1 (MQ=255) ggCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGCGGGGa < 4:340060/149‑1 (MQ=255) gCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGCGGGGAg < 4:195003/149‑1 (MQ=255) | TAGATGCTCACGCCAGCCGGTTTTTCCGCTCCGGCGTTGGCAGCCATGGGCAGCCAACCTTCGATGAAATCGGTCGGTTCAGCAGGAATCGGCTGCGGGCTCCAGCGCAGGCGGTTGGGG‑TTGATGCCACCCAGTGGCCCGCCGAGCGGCTGGCGCGCCAGGCGCTCGAAGCGTGGGTGCAAGGCAGAAGGGCGAATGCGGTACAGCCAGGTACGGCGCAGCTCGCTGCGGGCCATGGTGAACGCCGTGCCCGACAGCAGCTCGGCATACAGGCCATACGGGGCCTTCTGCGGGGAG > NC_002947/5244087‑5244383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |