Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,277,657	A→T	7.1%	E206D (GAA→GAT)	PP_4652 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,277,657	0	A	T	7.1%	248.8 / 7.4	85	E206D (GAA→GAT)	PP_4652	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (38/41);  new base T (3/3);  total (41/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

CGCCGGCGGTGGGCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATG  >  NC_002947/5277512‑5277801                                                                                                                                                  |                                                                                                                                                 cgcgGGCGGTGGGCGGATTCCTTTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCg                                                                                                                                               <  7:134174/149‑1 (MQ=255)          tGGGCGGCTTCCTGTATGCCTTTGTCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGTCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctggct                                                                                                                                      <  4:235479/149‑1 (MQ=255)           gggCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctggctg                                                                                                                                     <  6:283425/149‑1 (MQ=255)                   tCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGc                                                                                                                             >  4:136551/1‑149 (MQ=255)                      tGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCtt                                                                                                                           >  5:234936/1‑148 (MQ=255)                          tGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAtt                                                                                                                       <  2:393575/148‑1 (MQ=255)                          tGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTc                                                                                                                      <  1:298946/149‑1 (MQ=255)                             cTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCg                                                                                                                     >  7:293201/1‑147 (MQ=255)                             cTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCg                                                                                                                     >  5:160730/1‑147 (MQ=255)                             cTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCa                                                                                                                   <  1:315350/149‑1 (MQ=255)                             cTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCa                                                                                                                   <  3:46404/149‑1 (MQ=255)                                  gcagcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAtt                                                                                                                       >  1:159435/1‑140 (MQ=255)                                  gcagcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAtt                                                                                                                       <  2:159435/140‑1 (MQ=255)                                    agcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCAGCGATTCGATgctggc                                                                                                                                       >  3:30855/1‑122 (MQ=255)                                    agcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCAGCGATTCGATgctggc                                                                                                                                       <  4:30855/122‑1 (MQ=255)                                       aTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGa                                                                                                         <  8:293201/149‑1 (MQ=255)                                       aTGTGGGTGTACGGCCAGACGGTGGACTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGAGCGGCCAGGTCGCCCAGCGCGGTCGCGCCAGCATCGAATCGCTGCTGGCTGACATCCGCTTCATTCGCAGCAGGCCCg                                                                                                          >  8:188390/1‑148 (MQ=255)                                         gtgGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctgg                                                                                                                                        <  1:263642/116‑1 (MQ=255)                                         gtgGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctgg                                                                                                                                        >  2:263642/1‑116 (MQ=255)                                         gtgGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACa                                                                                                       >  2:81888/1‑149 (MQ=255)                                            ggTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATcc                                                                                                    >  7:111928/1‑149 (MQ=255)                                             gtgtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCt                                                                                                   >  6:78488/1‑149 (MQ=255)                                             gtgtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCACCCTCGAATAGCTGCTGGCTGGCATCCGCTTAAATCCCCGCCGGCCCCACAtaca                                                                                                   >  6:23797/1‑146 (MQ=255)                                               gtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCa                                                                                                                         <  4:65667/125‑1 (MQ=255)                                               gtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCg                                                                                                 >  5:243678/1‑149 (MQ=255)                                                 aCGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAtt                                                                                                                       <  3:261667/125‑1 (MQ=255)                                                 aCGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAtt                                                                                                                       >  4:261667/1‑125 (MQ=255)                                                    gCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGcc                                                                                            <  5:78488/149‑1 (MQ=255)                                                           ggTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                     <  5:23797/149‑1 (MQ=255)                                                           ggTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                     <  6:160730/149‑1 (MQ=255)                                                             tGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGTGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTg                                                                                   >  4:51498/1‑149 (MQ=255)                                                             tGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTg                                                                                   <  6:234936/149‑1 (MQ=255)                                                                 cTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCtt                                                                                                                           <  4:36305/105‑1 (MQ=255)                                                                 cTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAcc                                                                               >  4:100206/1‑149 (MQ=255)                                                                  ttGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCt                                                                              >  5:242490/1‑149 (MQ=255)                                                                        gCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                >  7:195135/1‑141 (MQ=255)                                                                        gCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                <  8:195135/141‑1 (MQ=255)                                                                          cATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCg                                                                      >  1:252610/1‑149 (MQ=255)                                                                                 tGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTAttgtt                                                               >  2:299/1‑149 (MQ=255)                                                                                  gCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                     >  7:265166/1‑126 (MQ=255)                                                                                  gCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                     <  8:265166/126‑1 (MQ=255)                                                                                    gTGCTGTCCCTGCTCCTGCCGGGGGGCGGCCAGGTCGCCCATCGCGGGCGCGCCAGGATCGAATCGCTTCTGGCTGGCATCCGCTTCATTCGCATCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTggg                                                            <  8:255894/149‑1 (MQ=255)                                                                                        tGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATAGTTGGGCg                                                          >  6:31970/1‑147 (MQ=255)                                                                                          aCCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGgc                                                                                               >  5:402146/1‑108 (MQ=255)                                                                                          aCCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGgc                                                                                               <  6:402146/108‑1 (MQ=255)                                                                                          aCCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGa                                                        <  6:242490/147‑1 (MQ=255)                                                                                          aCCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGa                                                        <  1:299/147‑1 (MQ=255)                                                                                            ccTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCa                                                    >  7:264080/1‑149 (MQ=255)                                                                                             cTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCAc                                                   >  5:248157/1‑149 (MQ=255)                                                                                             cTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATAGCTGCTGGCTTGCATCCGCTTCAATCGCAGCCGGCCCGAAATCCACGGCGCCATCTCGCTGGGCCTGTTAGCCGTATTGTTGGGCGGAGacac                                                   >  8:193403/1‑149 (MQ=255)                                                                                              tGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCg                                                          <  7:390034/141‑1 (MQ=255)                                                                                              tGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCg                                                          >  8:390034/1‑141 (MQ=255)                                                                                              tGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCAcc                                                  >  5:168453/1‑149 (MQ=255)                                                                                                 gCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATAGCTTCTGGCTGGCATCCGCTTCATTAGCAGCCCGCCCCACATCCACGGCGCCATCTCGCTGGACCTTTTAGCCGTATTGTTGGGCGGAGCCAccac                                                >  6:239192/1‑146 (MQ=255)                                                                                                  ccTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCCGCATCGAATCGCAGCTGGCTGGCATCCGCGTCAATAGCACCCGGCCCGACATACAACGCGCCAAAAAGCGGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACcccc                                               >  4:200935/1‑148 (MQ=255)                                                                                                  ccTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGcc                                               <  3:200935/148‑1 (MQ=255)                                                                                                   cTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCtt                                             <  2:98414/149‑1 (MQ=255)                                                                                                     gCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGCCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTtg                                            >  4:305827/1‑148 (MQ=255)                                                                                                      cAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATCGTTGGGCGGAGCCACCGCCTtgc                                           <  5:31970/148‑1 (MQ=255)                                                                                                         gcgcgcGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTtg                                            >  5:329545/1‑144 (MQ=255)                                                                                                         gcgcgcGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTtg                                            <  6:329545/144‑1 (MQ=255)                                                                                                          cgcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCg                                      <  6:168453/149‑1 (MQ=255)                                                                                                          cgcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCg                                      <  3:100206/149‑1 (MQ=255)                                                                                                           gcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                     <  8:119162/101‑1 (MQ=255)                                                                                                           gcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                     >  7:119162/1‑101 (MQ=255)                                                                                                           gcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGCCATCCGCTTCATTAGCAGCCGGCCCGACATCATCGGCGCCATATCGCTGGACCTCTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCgg                                     >  2:73529/1‑149 (MQ=255)                                                                                                           gcgcgGCAAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCAGCTACATTAGCAGAAGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCgg                                     >  5:297744/1‑149 (MQ=255)                                                                                                                gCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTAttt                                <  8:264080/149‑1 (MQ=255)                                                                                                                  cAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGc                              <  6:248157/149‑1 (MQ=255)                                                                                                                    ggTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGcc                             >  8:106493/1‑148 (MQ=255)                                                                                                                     gTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCaa                           <  6:243678/149‑1 (MQ=255)                                                                                                                        gCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAgg                         <  7:106493/148‑1 (MQ=255)                                                                                                                            aGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGAATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATc                    <  5:389691/149‑1 (MQ=255)                                                                                                                               gcgGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCctg                 <  2:252610/149‑1 (MQ=255)                                                                                                                                cgGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCctgc                <  5:239192/149‑1 (MQ=255)                                                                                                                                   gcgcgcCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                >  6:167550/1‑82 (MQ=255)                                                                                                                                   gcgcgcCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                <  5:167550/82‑1 (MQ=255)                                                                                                                                      agcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGc                                                >  2:243996/2‑111 (MQ=255)                                                                                                                                      agcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGc                                                <  1:243996/110‑1 (MQ=255)                                                                                                                                       gcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCg                                      >  2:282113/1‑120 (MQ=255)                                                                                                                                       gcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCg                                      <  1:282113/120‑1 (MQ=255)                                                                                                                                         cAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTgg                                                             <  2:277545/95‑1 (MQ=255)                                                                                                                                         cAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTgg                                                             >  1:277545/1‑95 (MQ=255)                                                                                                                                         cAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGc       <  1:81888/149‑1 (MQ=255)                                                                                                                                              tCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATg  >  3:268804/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                 CGCCGGCGGTGGGCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATG  >  NC_002947/5277512‑5277801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑