Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,277,661	C→A	7.1%	L208M (CTG→ATG)	PP_4652 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,277,661	0	C	A	7.1%	267.6 / 8.3	84	L208M (CTG→ATG)	PP_4652	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (38/40);  new base A (3/3);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

TGGGCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATGGGCCTTGG  >  NC_002947/5277521‑5277809                                                                                                                                             |                                                                                                                                                     tGGGCGGCTTCCTGTATGCCTTTGTCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGTCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctggct                                                                                                                                              <  4:235479/149‑1 (MQ=255)  gggCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctggctg                                                                                                                                             <  6:283425/149‑1 (MQ=255)          tCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGc                                                                                                                                     >  4:136551/1‑149 (MQ=255)             tGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCtt                                                                                                                                   >  5:234936/1‑148 (MQ=255)                 tGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAtt                                                                                                                               <  2:393575/148‑1 (MQ=255)                 tGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTc                                                                                                                              <  1:298946/149‑1 (MQ=255)                    cTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCg                                                                                                                             >  7:293201/1‑147 (MQ=255)                    cTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCg                                                                                                                             >  5:160730/1‑147 (MQ=255)                    cTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCa                                                                                                                           <  3:46404/149‑1 (MQ=255)                    cTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCa                                                                                                                           <  1:315350/149‑1 (MQ=255)                         gcagcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAtt                                                                                                                               >  1:159435/1‑140 (MQ=255)                         gcagcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAtt                                                                                                                               <  2:159435/140‑1 (MQ=255)                           agcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCAGCGATTCGATgctggc                                                                                                                                               <  4:30855/122‑1 (MQ=255)                           agcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCAGCGATTCGATgctggc                                                                                                                                               >  3:30855/1‑122 (MQ=255)                              aTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGa                                                                                                                 <  8:293201/149‑1 (MQ=255)                              aTGTGGGTGTACGGCCAGACGGTGGACTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGAGCGGCCAGGTCGCCCAGCGCGGTCGCGCCAGCATCGAATCGCTGCTGGCTGACATCCGCTTCATTCGCAGCAGGCCCg                                                                                                                  >  8:188390/1‑148 (MQ=255)                                gtgGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctgg                                                                                                                                                >  2:263642/1‑116 (MQ=255)                                gtgGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctgg                                                                                                                                                <  1:263642/116‑1 (MQ=255)                                gtgGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACa                                                                                                               >  2:81888/1‑149 (MQ=255)                                   ggTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATcc                                                                                                            >  7:111928/1‑149 (MQ=255)                                    gtgtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCt                                                                                                           >  6:78488/1‑149 (MQ=255)                                    gtgtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCACCCTCGAATAGCTGCTGGCTGGCATCCGCTTAAATCCCCGCCGGCCCCACAtaca                                                                                                           >  6:23797/1‑146 (MQ=255)                                      gtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCa                                                                                                                                 <  4:65667/125‑1 (MQ=255)                                      gtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCg                                                                                                         >  5:243678/1‑149 (MQ=255)                                        aCGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAtt                                                                                                                               <  3:261667/125‑1 (MQ=255)                                        aCGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAtt                                                                                                                               >  4:261667/1‑125 (MQ=255)                                           gCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGcc                                                                                                    <  5:78488/149‑1 (MQ=255)                                                  ggTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                             <  6:160730/149‑1 (MQ=255)                                                  ggTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                             <  5:23797/149‑1 (MQ=255)                                                    tGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGTGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTg                                                                                           >  4:51498/1‑149 (MQ=255)                                                    tGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTg                                                                                           <  6:234936/149‑1 (MQ=255)                                                        cTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCtt                                                                                                                                   <  4:36305/105‑1 (MQ=255)                                                        cTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAcc                                                                                       >  4:100206/1‑149 (MQ=255)                                                         ttGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCt                                                                                      >  5:242490/1‑149 (MQ=255)                                                               gCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                        >  7:195135/1‑141 (MQ=255)                                                               gCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                        <  8:195135/141‑1 (MQ=255)                                                                 cATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCg                                                                              >  1:252610/1‑149 (MQ=255)                                                                        tGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTAttgtt                                                                       >  2:299/1‑149 (MQ=255)                                                                         gCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                             <  8:265166/126‑1 (MQ=255)                                                                         gCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                             >  7:265166/1‑126 (MQ=255)                                                                           gTGCTGTCCCTGCTCCTGCCGGGGGGCGGCCAGGTCGCCCATCGCGGGCGCGCCAGGATCGAATCGCTTCTGGCTGGCATCCGCTTCATTCGCATCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTggg                                                                    <  8:255894/149‑1 (MQ=255)                                                                               tGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATAGTTGGGCg                                                                  >  6:31970/1‑147 (MQ=255)                                                                                 aCCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGgc                                                                                                       <  6:402146/108‑1 (MQ=255)                                                                                 aCCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGgc                                                                                                       >  5:402146/1‑108 (MQ=255)                                                                                 aCCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGa                                                                <  6:242490/147‑1 (MQ=255)                                                                                 aCCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGa                                                                <  1:299/147‑1 (MQ=255)                                                                                   ccTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCa                                                            >  7:264080/1‑149 (MQ=255)                                                                                    cTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCAc                                                           >  5:248157/1‑149 (MQ=255)                                                                                    cTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATAGCTGCTGGCTTGCATCCGCTTCAATCGCAGCCGGCCCGAAATCCACGGCGCCATCTCGCTGGGCCTGTTAGCCGTATTGTTGGGCGGAGacac                                                           >  8:193403/1‑149 (MQ=255)                                                                                     tGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCg                                                                  >  8:390034/1‑141 (MQ=255)                                                                                     tGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCg                                                                  <  7:390034/141‑1 (MQ=255)                                                                                     tGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCAcc                                                          >  5:168453/1‑149 (MQ=255)                                                                                        gCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATAGCTTCTGGCTGGCATCCGCTTCATTAGCAGCCCGCCCCACATCCACGGCGCCATCTCGCTGGACCTTTTAGCCGTATTGTTGGGCGGAGCCAccac                                                        >  6:239192/1‑146 (MQ=255)                                                                                         ccTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCCGCATCGAATCGCAGCTGGCTGGCATCCGCGTCAATAGCACCCGGCCCGACATACAACGCGCCAAAAAGCGGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACcccc                                                       >  4:200935/1‑148 (MQ=255)                                                                                         ccTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGcc                                                       <  3:200935/148‑1 (MQ=255)                                                                                          cTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCtt                                                     <  2:98414/149‑1 (MQ=255)                                                                                            gCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGCCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTtg                                                    >  4:305827/1‑148 (MQ=255)                                                                                             cAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATCGTTGGGCGGAGCCACCGCCTtgc                                                   <  5:31970/148‑1 (MQ=255)                                                                                                gcgcgcGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTtg                                                    >  5:329545/1‑144 (MQ=255)                                                                                                gcgcgcGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTtg                                                    <  6:329545/144‑1 (MQ=255)                                                                                                 cgcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCg                                              <  6:168453/149‑1 (MQ=255)                                                                                                 cgcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCg                                              <  3:100206/149‑1 (MQ=255)                                                                                                  gcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                             >  7:119162/1‑101 (MQ=255)                                                                                                  gcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGc                                                                                             <  8:119162/101‑1 (MQ=255)                                                                                                  gcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGCCATCCGCTTCATTAGCAGCCGGCCCGACATCATCGGCGCCATATCGCTGGACCTCTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCgg                                             >  2:73529/1‑149 (MQ=255)                                                                                                  gcgcgGCAAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCAGCTACATTAGCAGAAGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCgg                                             >  5:297744/1‑149 (MQ=255)                                                                                                       gCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTAttt                                        <  8:264080/149‑1 (MQ=255)                                                                                                         cAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGc                                      <  6:248157/149‑1 (MQ=255)                                                                                                           ggTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGcc                                     >  8:106493/1‑148 (MQ=255)                                                                                                            gTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCaa                                   <  6:243678/149‑1 (MQ=255)                                                                                                               gCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAgg                                 <  7:106493/148‑1 (MQ=255)                                                                                                                   aGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGAATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATc                            <  5:389691/149‑1 (MQ=255)                                                                                                                      gcgGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCctg                         <  2:252610/149‑1 (MQ=255)                                                                                                                       cgGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCctgc                        <  5:239192/149‑1 (MQ=255)                                                                                                                          gcgcgcCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                        >  6:167550/1‑82 (MQ=255)                                                                                                                          gcgcgcCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                        <  5:167550/82‑1 (MQ=255)                                                                                                                             agcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGc                                                        >  2:243996/2‑111 (MQ=255)                                                                                                                             agcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGc                                                        <  1:243996/110‑1 (MQ=255)                                                                                                                              gcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCg                                              >  2:282113/1‑120 (MQ=255)                                                                                                                              gcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCg                                              <  1:282113/120‑1 (MQ=255)                                                                                                                                cAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTgg                                                                     <  2:277545/95‑1 (MQ=255)                                                                                                                                cAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTgg                                                                     >  1:277545/1‑95 (MQ=255)                                                                                                                                cAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGc               <  1:81888/149‑1 (MQ=255)                                                                                                                                     tCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATg          >  3:268804/1‑149 (MQ=255)                                                                                                                                            gctgctGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGGATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGTCCGGGCCGATGCGCGTTg   >  3:17118/1‑149 (MQ=255)                                                                                                                                             ctgctgGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATGGGCCTTgg  <  8:111928/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                     TGGGCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATGGGCCTTGG  >  NC_002947/5277521‑5277809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑