Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,291,249 T→A 5.6% Y283N (TAT→AAT)  PP_4664 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,291,2490TA 5.6% 224.5 / 3.0 71Y283N (TAT→AAT) PP_4664LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (31/36);  new base A (2/2);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

CGGGCGGGGCCGGAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTGCGGCGCGGCCGTTGGGCCTATGGT  >  NC_002947/5291103‑5291390
                                                                                                                                                  |                                                                                                                                              
cgggcgggGCCGGAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGCGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTAt                                                                                                                                              <  6:425556/149‑1 (MQ=255)
   gcgggGCCGGAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGcc                                                                                                                                           <  8:254473/149‑1 (MQ=255)
       ggCCGGAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAg                                                                                                                                       <  5:108902/149‑1 (MQ=255)
        gCCGGAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAg                                                                                                                                       >  3:190442/1‑148 (MQ=255)
         ccGGAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGaa                                                                                                                                     >  1:27166/1‑149 (MQ=255)
          cGGAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAAc                                                                                                                                    >  1:186016/1‑149 (MQ=255)
          cGGAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAAc                                                                                                                                    <  8:114583/149‑1 (MQ=255)
          cGGAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAAc                                                                                                                                    <  2:203270/149‑1 (MQ=255)
           ggAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACt                                                                                                                                   <  7:325160/149‑1 (MQ=255)
            gAATCGGGGTGGTGCCACTGTCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTg                                                                                                                                  >  5:342278/1‑149 (MQ=255)
                 ggggTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATAGCCCGGCAATGCCGCaaaatcc                                                                                                                                  <  3:89456/144‑4 (MQ=255)
                 ggggTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATAGCCCGGCAATGCCGCaaaatcc                                                                                                                                  >  4:89456/1‑141 (MQ=255)
                   ggtggtGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCa                                                                                                                           <  4:190442/149‑1 (MQ=255)
                    gtggtgCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCAc                                                                                                                          <  4:199429/149‑1 (MQ=255)
                        tgCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCAc                                                                                                                          <  2:102849/145‑1 (MQ=255)
                        tgCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCAc                                                                                                                          >  1:102849/1‑145 (MQ=255)
                         gCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCa                                                                                                                           <  3:287355/143‑1 (MQ=255)
                         gCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCa                                                                                                                           >  4:287355/1‑143 (MQ=255)
                           cACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTagc                                                                                                                   <  2:186016/149‑3 (MQ=255)
                           cACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTAcc                                                                                                                   <  1:134536/149‑1 (MQ=255)
                            aCTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCg                                                                                                                  >  3:222646/1‑149 (MQ=255)
                                gcagcaGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGc                                                                                                                <  1:244410/147‑1 (MQ=255)
                                gcagcaGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCt                                                                                                               <  8:374735/148‑1 (MQ=255)
                                   gcagTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCGACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCAGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGAcg                                                                                                           >  7:57485/1‑149 (MQ=255)
                                    cagTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGAcgc                                                                                                          <  2:27166/149‑1 (MQ=255)
                                       tACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACgcgc                                                                                                        >  8:100786/1‑148 (MQ=255)
                                          aGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAgg                                                                                                    >  5:299783/1‑149 (MQ=255)
                                            cgcTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGgc                                                                                                  >  5:411986/1‑149 (MQ=255)
                                              cTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGgcgc                                                                                                <  1:383083/149‑1 (MQ=255)
                                                 gCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGcaca                                                                                             >  2:273912/1‑149 (MQ=255)
                                                 gCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATAGCCCGGCAATGCCg                                                                                                                                          <  3:109743/104‑1 (MQ=255)
                                                 gCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATAGCCCGGCAATGCCg                                                                                                                                          >  4:109743/1‑104 (MQ=255)
                                                    agtcATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCGATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCAcacca                                                                                          <  6:342278/146‑1 (MQ=255)
                                                        aTGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCa                                                                                                                                        >  4:233005/1‑99 (MQ=255)
                                                        aTGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCa                                                                                                                                        <  3:233005/99‑1 (MQ=255)
                                                          gctgcCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑Tc                                                                                    >  4:209810/1‑148 (MQ=255)
                                                            tgcCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAg                                                                                 >  8:328741/1‑149 (MQ=255)
                                                              ccGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAg                                                                                 <  7:100786/147‑1 (MQ=255)
                                                                gTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGTGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGa                                                                                  <  2:248206/144‑1 (MQ=255)
                                                                gTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGTGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGa                                                                                  >  1:248206/1‑144 (MQ=255)
                                                                gTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTa                                                                                                                               <  1:562/100‑1 (MQ=255)
                                                                gTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTa                                                                                                                               >  2:562/1‑100 (MQ=255)
                                                                  tGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAAc                                                                           >  5:258630/1‑149 (MQ=255)
                                                                   gCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAAcc                                                                          >  3:3061/1‑149 (MQ=255)
                                                                         ggTGGCACTGCAGGAGCCATGGGCCGACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCt                                                                      <  8:57485/147‑1 (MQ=255)
                                                                           tGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCa                                                                  >  8:264288/1‑149 (MQ=255)
                                                                              cACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAg                                                               >  8:390411/1‑149 (MQ=255)
                                                                                   cAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGcgcg                                                           >  3:325928/1‑148 (MQ=255)
                                                                                   cAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGcgcg                                                           <  4:325928/148‑1 (MQ=255)
                                                                                     ggAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGc                                                        <  7:390411/149‑1 (MQ=255)
                                                                                     ggAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGc                                                        <  8:304312/149‑1 (MQ=255)
                                                                                           atttgCCAACCGACGTTTGCTGGTGAGCGCACGGTATTTTGCGGCATTTCCGGGCTATGCCGAAGAACTGGTACCCACCTTTACAGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGTGCAGacac                                                  >  2:297638/5‑149 (MQ=255)
                                                                                            tGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTGTTCGAGGCAAACCGCCTGCCATAGCGCGGGCAGacac                                                  <  1:273912/149‑1 (MQ=255)
                                                                                            tGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACAcc                                                 >  2:47254/1‑149 (MQ=255)
                                                                                              ggCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCt                                                <  5:294189/148‑1 (MQ=255)
                                                                                               gCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTg                                               >  8:282813/1‑148 (MQ=255)
                                                                                                ccAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGa                                             >  2:168944/1‑149 (MQ=255)
                                                                                                 cAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGAc                                            >  5:233145/1‑149 (MQ=255)
                                                                                                     cgccgGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCg                                        <  7:282813/149‑1 (MQ=255)
                                                                                                          gTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGccc                                   <  7:264288/149‑1 (MQ=255)
                                                                                                             gCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAg                                 <  8:55016/148‑1 (MQ=255)
                                                                                                                  tgtgCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACt                           >  1:38590/1‑149 (MQ=255)
                                                                                                                   gtgCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTg                          <  3:209810/149‑1 (MQ=255)
                                                                                                                     gcgcACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGc                                                                    >  6:422343/1‑105 (MQ=255)
                                                                                                                     gcgcACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGc                                                                    <  5:422343/105‑1 (MQ=255)
                                                                                                                      cgcACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTgcgg                       <  4:222646/149‑1 (MQ=255)
                                                                                                                      cgcACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTgcg                        <  7:328741/148‑1 (MQ=255)
                                                                                                                         aCGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTgcggcgc                    >  2:45138/1‑149 (MQ=255)
                                                                                                                           gggATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTgcggcgcgg                  <  6:258630/149‑1 (MQ=255)
                                                                                                                                   gCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTGCGGCGCGGCCGTTgg           <  1:45138/148‑1 (MQ=255)
                                                                                                                                     ggCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTGCGGCGCGGCCGTTGGGcc        >  5:60842/1‑149 (MQ=255)
                                                                                                                                                  tATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTGCGGCGCGGCCGTTGGGCCTATGGt  <  6:116759/142‑1 (MQ=255)
                                                                                                                                                  tATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTGCGGCGCGGCCGTTGGGCCTATGGt  >  5:116759/1‑142 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CGGGCGGGGCCGGAATCGGGGTGGTGCCACTGGCAGCAGTACAGCGCTGGCAAGGCATGCTGCCGTTGCACTGGGTGGCACTGCAGGAGCCATGGGCCAACCGCCGGTTGCTGGTGTGCGCACGGGATTTTGCGGCATTGCCGGGCTATGCCGCAGAACTGGTAGCCACCTTTACCGGCTGACGCGCAAGGGCGCACACCACTG‑TCGAGGCAAACCGCCTGCCATAGCGCGGGCAGACACCTGGACGTCGAGCCCAGGCAACTGCGGCGCGGCCGTTGGGCCTATGGT  >  NC_002947/5291103‑5291390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑