Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,325,511	T→A	9.8%	noncoding (133/149 nt)	PP_mr52 →	PrrF ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,325,511	0	T	A	9.8%	203.3 / 10.2	71	noncoding (133/149 nt)	PP_mr52	PrrF ncRNA
Reads supporting (aligned to +/- strand):  ref base T (34/30);  new base A (5/2);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TAATCGGGGCAAAGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAGCAGCAATA  >  NC_002947/5325364‑5325656                                                                                                                                                    |                                                                                                                                                  taatCGGGGCAAAGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAAtt                                                                                                                                                  >  6:77626/1‑149 (MQ=255)         gCAAAGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGt                                                                                                                                          <  5:77626/149‑1 (MQ=255)         gCAAAGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGt                                                                                                                                          <  6:42430/149‑1 (MQ=255)           aaaGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCt                                                                                                                                        >  2:129512/1‑149 (MQ=255)                 aTTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCttttttt                                                                                                                                  <  1:129512/149‑1 (MQ=255)                     aCGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCttttttgcct                                                                                                                               <  4:346008/148‑5 (MQ=255)                          tAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGcc                                                                                                                         >  8:45089/1‑149 (MQ=255)                              tGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGcc                                                                                                                                              >  1:167679/1‑124 (MQ=255)                              tGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGcc                                                                                                                                              <  2:167679/124‑1 (MQ=255)                                 gaATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCa                                                                                                                   <  6:331863/148‑1 (MQ=255)                                      gTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGAt                                                                                                               >  2:253142/1‑147 (MQ=255)                                      gTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAAGCACGGTTATTGACCCGGCTATTTGCCGTGTCTTGTTTTGCATGTGCCTTTTCAGGAt                                                                                                               >  8:282230/1‑147 (MQ=38)                                       ttattattaTCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAg                                                                                                            <  7:45089/149‑1 (MQ=255)                                            attatCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGcgccg                                                                                                       >  6:235003/1‑149 (MQ=255)                                             ttatCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGGTCAGcgccg                                                                                                       >  2:184340/1‑148 (MQ=255)                                             ttatCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGcgccg                                                                                                       <  8:211714/148‑1 (MQ=255)                                                   cAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTgc                                                                                                >  5:419083/1‑149 (MQ=255)                                                   cAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCATGTGCCTTTTCAGGATCAGCGCCGCAGCTgc                                                                                                >  8:97743/1‑149 (MQ=255)                                                    aaCTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTgcg                                                                                               <  5:167765/149‑1 (MQ=255)                                                        ggTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGcac                                                                                            >  6:178649/1‑148 (MQ=255)                                                          tCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACaa                                                                                          >  6:133080/1‑148 (MQ=255)                                                              gagaCTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATc                                                                                     >  4:150264/1‑149 (MQ=255)                                                              gagaCTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATc                                                                                     <  6:419083/149‑1 (MQ=255)                                                                     gTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTcc                                                                              >  2:212467/1‑149 (MQ=255)                                                                           tAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATc                                                                        >  2:200913/1‑149 (MQ=255)                                                                              gCTAAGAGCCCCTGATTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATcgcc                                                                     >  6:234579/1‑149 (MQ=255)                                                                                   gagCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGca                                                                                             <  5:199117/120‑1 (MQ=255)                                                                                   gagCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGca                                                                                             >  6:199117/1‑120 (MQ=255)                                                                                       cccTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATc                                                                                     >  6:265676/1‑124 (MQ=255)                                                                                       cccTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATc                                                                                     <  5:265676/124‑1 (MQ=255)                                                                                       cccTGATTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTAc                                                            <  5:234579/149‑1 (MQ=255)                                                                                            gTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACcac                                                         <  4:120629/147‑1 (MQ=255)                                                                                                 gACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTc                                                                                                                    >  8:367721/1‑83 (MQ=255)                                                                                                 gACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATc                                                                                     <  5:164499/114‑1 (MQ=255)                                                                                                 gACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATc                                                                                     >  6:164499/1‑114 (MQ=255)                                                                                                      tcAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATTGCCGCCGTGTACCACACATAATCAGcc                                             >  6:38119/1‑149 (MQ=255)                                                                                                      tcAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACcacac                                                       <  8:165065/139‑1 (MQ=255)                                                                                                      tcAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACcacac                                                       >  7:165065/1‑139 (MQ=255)                                                                                                      tcAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGcc                                             >  7:122907/1‑149 (MQ=255)                                                                                                        aGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGcc                                             <  5:133080/147‑1 (MQ=255)                                                                                                        aGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGc                                              <  7:282230/146‑1 (MQ=255)                                                                                                        aGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTc                                           <  1:253142/149‑1 (MQ=255)                                                                                                             aTCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCa                                       <  8:122907/148‑1 (MQ=255)                                                                                                              tctcCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAg                                      <  1:200913/148‑1 (MQ=255)                                                                                                                tcctcATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATc                                                                                     <  7:86435/99‑1 (MQ=255)                                                                                                                tcctcATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATc                                                                                     >  8:86435/1‑99 (MQ=255)                                                                                                                     atcaGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGtt                              >  4:154952/1‑149 (MQ=255)                                                                                                                        aGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCGTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTtcgc                           >  2:73443/1‑149 (MQ=255)                                                                                                                        aGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATTGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTtcgc                           <  5:38119/149‑1 (MQ=255)                                                                                                                        aGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTtcgc                           <  5:244355/149‑1 (MQ=255)                                                                                                                         ggCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTtcgct                          >  6:152895/1‑149 (MQ=255)                                                                                                                            tAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACca                                                          <  3:49703/114‑1 (MQ=255)                                                                                                                            tAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACca                                                          >  4:49703/1‑114 (MQ=255)                                                                                                                            tAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCGGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTtcgctcgc                       >  5:179174/1‑149 (MQ=255)                                                                                                                               taaCGGTTATTGACCCGGAAATTTGCCGGGTCTTTTATTGCCTGTGACTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTCCTCCGGCATCTCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCa                    >  6:368111/3‑149 (MQ=255)                                                                                                                               tCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACAACACATAATCAGCCTCGCCACGCTCGGTACGCTCGccac                    >  8:364226/1‑147 (MQ=255)                                                                                                                                 aCGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTAcc                                                           <  6:239759/108‑1 (MQ=255)                                                                                                                                 aCGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTAcc                                                           >  5:239759/1‑108 (MQ=255)                                                                                                                                   aaaaaaagACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCt                 >  2:14403/8‑148 (MQ=255)                                                                                                                                     aaaaagACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTcag              >  5:164622/6‑149 (MQ=255)                                                                                                                                       aTTGACCAGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACAGAATCAGCCTCGCCAGGCTCGGTTCGCGCGCGCAccgcggg             >  4:265656/1‑143 (MQ=255)                                                                                                                                         agACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCATCGCCAGGCTCGGTTCGCTCGCCCACCTcagccag          >  6:68053/2‑149 (MQ=255)                                                                                                                                          gACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTcagccag          <  1:212467/148‑1 (MQ=255)                                                                                                                                          gACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTcagc             <  4:147968/145‑1 (MQ=255)                                                                                                                                          gACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTcagc             >  3:142007/1‑145 (MQ=255)                                                                                                                                          gACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTcagc             >  3:147968/1‑145 (MQ=255)                                                                                                                                          gACCCGGCAAATTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTcagc             <  4:142007/145‑1 (MQ=255)                                                                                                                                            cccGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGCTCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCcagcag       <  1:184340/149‑1 (MQ=255)                                                                                                                                             ccGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCcagcag       >  4:85064/1‑148 (MQ=255)                                                                                                                                              cGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAgcagca     <  5:178649/149‑1 (MQ=255)                                                                                                                                                 cAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAGCAGCAATa  <  7:97743/149‑1 (MQ=255)                                                                                                                                                  aaTTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAGCAGCAATa  >  8:186009/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TAATCGGGGCAAAGGGATTGACGGCTAAATGAGAATTGTTATTATTATCGCAACTGGTCGCGAGACTGGTTGGATAAGCTAAGAGCCCCTGGTTCGGACTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTGACCCGGCAATTTGCCGGGTCTTTTTTTGCCTGTGCCTTTTCAGGATCAGCGCCGCAGCTGCGCACAATAATCCTGCTCCGGCATCGCCGCCGTGTACCACACATAATCAGCCTCGCCAGGCTCGGTTCGCTCGCCCACCTCAGCCAGCAGCAATA  >  NC_002947/5325364‑5325656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑