Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	536,280	A→G	10.0%	noncoding (178/190 nt)	PP_mr06 ←	P27 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	536,280	0	A	G	10.0%	214.6 / 12.3	80	noncoding (178/190 nt)	PP_mr06	P27 ncRNA
Reads supporting (aligned to +/- strand):  ref base A (40/32);  new base G (5/3);  total (45/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

GCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCC  >  NC_002947/536136‑536427                                                                                                                                                 |                                                                                                                                                     gCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTc                                                                                                                                                   >  4:385879/1‑148 (MQ=255) gCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTc                                                                                                                                                   <  3:385879/148‑1 (MQ=255)   ccTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTcgcg                                                                                                                                                >  3:314882/1‑149 (MQ=255)              aCCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCaa                                                                                                                                      >  5:145979/1‑148 (MQ=255)                cTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGCGCCGCCa                                                                                                                                          <  5:285442/142‑1 (MQ=255)                cTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACTGGTCGCGCCGCCa                                                                                                                                          >  6:285442/1‑142 (MQ=255)                 tGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAAt                                                                                                                                  >  4:28938/1‑149 (MQ=255)                 tGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCAGAGCACCACCATGGGCCAAGGTCTGGTCATCGATCAGAGCTCGCTGAACCTGAAAGAACAGGGCGGCGCGACCACTCGCCCAGCCAGCAAAAAt                                                                                                                                  >  4:86062/1‑149 (MQ=255)                  gAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAAtt                                                                                                                                 >  6:107617/1‑149 (MQ=255)                   aaGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTg                                                                                                                                <  5:107617/149‑1 (MQ=255)                    aGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCCTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTgg                                                                                                                               <  4:314882/149‑1 (MQ=255)                         aTCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTcc                                                                                                                          >  3:76707/1‑149 (MQ=255)                            aaGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcctg                                                                                                                       >  2:85603/1‑149 (MQ=255)                             aGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCcctgc                                                                                                                      <  4:76707/149‑1 (MQ=255)                                   cTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCa                                                                                                                                          <  8:154117/123‑1 (MQ=255)                                   cTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCa                                                                                                                                          >  7:154117/1‑123 (MQ=255)                                    ttCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCgg                                                                                                               >  7:80134/1‑149 (MQ=255)                                       cTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCggggg                                                                                                            <  7:300523/149‑1 (MQ=255)                                       cTCGAAAGGTATCTACGTTAAGCGCGTTACCCAGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCgggg                                                                                                             <  3:86062/148‑1 (MQ=255)                                        tCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGc                                                                                                           >  3:32292/1‑149 (MQ=255)                                          gAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCCGCCAGGCagggacc                                                                                                          <  3:115992/148‑4 (MQ=255)                                                 aTCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCaa                                                                                                    <  7:372944/147‑1 (MQ=255)                                                 aTCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCaa                                                                                                    >  8:372944/1‑147 (MQ=255)                                                      cGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCg                                                                                               <  4:125899/147‑1 (MQ=255)                                                         tAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgccgc                                                                                                                                            <  7:286181/99‑1 (MQ=255)                                                         tAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGcgccgc                                                                                                                                            >  8:286181/1‑99 (MQ=255)                                                          aaGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAggcg                                                                                         >  3:148635/1‑149 (MQ=255)                                                                 ttACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAg                                                                                 <  4:32292/149‑1 (MQ=255)                                                                   aCCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGc                                                                                >  2:200509/1‑148 (MQ=255)                                                                     ccTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCGATCCAAGACCGTAGGCG‑GCGCAAGCCt                                                                              >  4:237977/1‑148 (MQ=255)                                                                      cTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATcc                                                                                                      <  7:388709/124‑1 (MQ=255)                                                                      cTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATcc                                                                                                      >  8:388709/1‑124 (MQ=255)                                                                           caccacCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAAc                                                                        >  4:57900/1‑148 (MQ=255)                                                                           caccacCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACa                                                                       <  1:319495/149‑1 (MQ=255)                                                                              caccaTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACAt                                                                      >  6:245024/1‑147 (MQ=255)                                                                               accaTGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAATCCTTAAAACATCAg                                                                   <  7:351880/149‑1 (MQ=255)                                                                                      gCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAATTTGGGGTCCCTGCCTGGCGGGGGCTATCAAAGACCGTAGGCG‑GCGCAAGCCTTAAAGCATCAGACCaag                                                             >  7:166034/1‑146 (MQ=255)                                                                                       cccAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACg                                                             >  4:205753/1‑147 (MQ=255)                                                                                       cccAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAAAATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGATATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATAAGCCAACg                                                             >  4:22780/1‑147 (MQ=255)                                                                                           ggTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATg                                                       <  3:28938/149‑1 (MQ=255)                                                                                            gTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATgg                                                      >  1:61445/1‑149 (MQ=255)                                                                                               tGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTaaa                                                                          <  6:253786/126‑1 (MQ=255)                                                                                               tGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTaaa                                                                          >  5:253786/1‑126 (MQ=255)                                                                                                 gTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTc                                                 >  8:346281/1‑149 (MQ=255)                                                                                                  tCATCGATCAGAGCTCGCTGAACGTGAAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GTGCATGCCTTAAATCATCAGCCGACGCAGATGGTGCTcc                                                >  6:183265/1‑149 (MQ=255)                                                                                                   cATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTccc                                               >  1:287330/1‑149 (MQ=255)                                                                                                    aTCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTccc                                               >  7:210699/1‑148 (MQ=255)                                                                                                     tCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGa                                             <  5:382058/149‑1 (MQ=255)                                                                                                     tCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGa                                             >  4:367334/1‑149 (MQ=255)                                                                                                       gATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGCGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGAt                                            <  1:85603/149‑1 (MQ=255)                                                                                                        aTCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                          <  8:80134/149‑1 (MQ=255)                                                                                                         tCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCg                                         >  5:405660/1‑149 (MQ=255)                                                                                                            gagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGTGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTa                                      >  2:192180/1‑149 (MQ=255)                                                                                                            gagCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTa                                      <  7:346281/149‑1 (MQ=255)                                                                                                              gCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAc                                     <  8:210699/148‑1 (MQ=255)                                                                                                               cTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTAcc                                    >  6:320797/1‑148 (MQ=255)                                                                                                                tCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCg                                   >  6:382371/1‑148 (MQ=255)                                                                                                                  gCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGaa                                 <  6:393907/148‑1 (MQ=255)                                                                                                                  gCTGAACGTGTAAGAACAAGCCGGCGCGAACAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGTCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGaa                                 <  3:22780/148‑1 (MQ=255)                                                                                                                   cTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATc                               <  1:200509/149‑1 (MQ=255)                                                                                                                       aCGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGGTAACGAATcggga                           <  3:205753/149‑5 (MQ=255)                                                                                                                           gtAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                          <  4:221091/130‑1 (MQ=255)                                                                                                                           gtAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTc                                          >  3:221091/1‑130 (MQ=255)                                                                                                                            tAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGCCCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCCCGCAGAGGGTGCTCCCGAGTCGTTACCGAATCAgcaacac                       >  6:263697/1‑142 (MQ=255)                                                                                                                             aaGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCaa                      >  2:373402/1‑148 (MQ=255)                                                                                                                               gAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTg                                                    <  6:259502/116‑1 (MQ=255)                                                                                                                               gAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTg                                                    >  5:259502/1‑116 (MQ=255)                                                                                                                               gAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAAc                   <  2:287330/149‑1 (MQ=255)                                                                                                                                    tGGCGGCGCGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACAt              >  2:398734/1‑149 (MQ=255)                                                                                                                                    tGGCGGCGCGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACAt              >  4:345864/1‑149 (MQ=255)                                                                                                                                    tGGCAGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAg                             <  2:30994/134‑1 (MQ=255)                                                                                                                                    tGGCAGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAg                             >  1:30994/1‑134 (MQ=255)                                                                                                                                     ggcggcGCGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGa                 >  2:29142/1‑145 (MQ=255)                                                                                                                                     ggcggcGCGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGa                 <  1:29142/145‑1 (MQ=255)                                                                                                                                      gcggcgCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATcc            >  8:44029/1‑149 (MQ=255)                                                                                                                                         gcgcGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATcc            <  8:155652/146‑1 (MQ=255)                                                                                                                                         gcgcGACTGGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATcc            >  7:155652/1‑146 (MQ=255)                                                                                                                                           gcgACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCt       >  8:41470/1‑149 (MQ=255)                                                                                                                                            cgACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTc      <  5:382371/149‑1 (MQ=255)                                                                                                                                                cAGTCGCGCCGCCAGCAAAAATCGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGcc  <  6:145979/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     GCCCTGATCGCTGACCTGAAGCGTATCAAGCCAGCTTCCTCGAAAGGTATCTACGTTAAGCGCGTTACCCTGAGCACCACCATGGGCCCAGGTCTGGTCATCGATCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCG‑GCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCC  >  NC_002947/536136‑536427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑