Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,345,977 G→A 7.4% V459M (GTG→ATG) ‡ acsA‑II → acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,345,9770GA 7.4% 194.7 / 3.2 67V459M (GTG→ATG) ‡acsA‑IIacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (32/30);  new base A (2/3);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCAGACCGAAACCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGAT  >  NC_002947/5345830‑5346125
                                                                                                                                                   |                                                                                                                                                    
tggCAGACCGAAACCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGt                                                                                                                                                     <  1:383183/149‑1 (MQ=255)
        cGAAACCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAAACCGGCTGCGCCACCCAGACGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCAGCACGCCGGCgc                                                                                                                                             >  6:28231/1‑148 (MQ=255)
          aaaCCGGCGGCATCATGCTCACCCCTCTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATc                                                                                                                                           <  1:400319/149‑1 (MQ=255)
          aaaCCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATc                                                                                                                                           <  8:264138/149‑1 (MQ=255)
           aaCCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCAGCACGCCGGCgccgg                                                                                                                                          <  5:28231/149‑5 (MQ=255)
            aCCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCAGCACGCCGGCgccggg                                                                                                                                         <  6:96075/149‑6 (MQ=255)
             ccGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCaaa                                                                                                                                        >  3:379469/1‑149 (MQ=255)
             ccGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCaaa                                                                                                                                        <  6:97961/149‑1 (MQ=255)
             ccGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCaaa                                                                                                                                        >  3:287852/1‑149 (MQ=255)
             ccGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCa                                                                                                                                          >  5:97961/1‑147 (MQ=255)
                 cggcATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCa                                                                                                                                    <  2:71385/149‑1 (MQ=255)
                    catcatGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCt                                                                                                                                 >  8:192915/1‑149 (MQ=255)
                    catcatGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCt                                                                                                                                 >  5:287787/1‑149 (MQ=255)
                    catcatGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCt                                                                                                                                 <  4:287852/149‑1 (MQ=255)
                        atGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGc                                                                                                                              <  6:26702/148‑1 (MQ=255)
                              aCCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCaaa                                                                                                                                        <  5:181871/132‑1 (MQ=255)
                              aCCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCaaa                                                                                                                                        >  6:181871/1‑132 (MQ=255)
                              aCCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGc                                                                                                                        <  2:339763/148‑1 (MQ=255)
                                  cACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATc                                                                                                                   >  1:367081/1‑149 (MQ=255)
                                       cccGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGc                                                                                                                <  6:287787/147‑1 (MQ=255)
                                        ccGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGc                                                                                                             <  7:192915/149‑1 (MQ=255)
                                          ggCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGt                                                                                                           >  3:161749/1‑149 (MQ=255)
                                            cacacaGTCACTCAAACCCGTCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCt                                                                                                         <  5:410424/149‑1 (MQ=255)
                                                     aCTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGac                                                                                                 >  3:261625/1‑148 (MQ=255)
                                                       tCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCAGCACGCCGGCgccg                                                                                                                                           <  8:163713/104‑4 (MQ=255)
                                                       tCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCAGCACGCCGGCgccg                                                                                                                                           >  7:163713/1‑101 (MQ=255)
                                                         aaaCCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGAccacca                                                                                            >  2:402597/1‑149 (MQ=255)
                                                                       cACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGac                                                                               <  2:182738/148‑1 (MQ=255)
                                                                       cACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGac                                                                               >  1:182738/1‑148 (MQ=255)
                                                                        aCCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGacac                                                                             >  8:11036/1‑149 (MQ=255)
                                                                         cccAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACAcc                                                                            <  7:309857/149‑1 (MQ=255)
                                                                               cGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCGCGGGCAGATCCGCATATTCTACTGCGACGACCAGCGGATGGTCGACACCTACTTc                                                                      <  4:379469/149‑1 (MQ=255)
                                                                                      cGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGcccc                                                               >  7:223689/1‑149 (MQ=255)
                                                                                      cGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGcccc                                                               >  5:62051/1‑149 (MQ=255)
                                                                                      cGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTCGCGATCAACGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGcccc                                                               >  6:413978/1‑149 (MQ=255)
                                                                                        gCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGc                                                                                                                                               <  6:275658/67‑1 (MQ=255)
                                                                                        gCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGc                                                                                                                                               >  5:275658/1‑67 (MQ=255)
                                                                                          gTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTAc                                                                                                       <  2:44961/105‑1 (MQ=255)
                                                                                          gTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTAc                                                                                                       >  1:44961/1‑105 (MQ=255)
                                                                                            gCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGacc                                                                                                <  4:292106/110‑1 (MQ=255)
                                                                                            gCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGacc                                                                                                >  3:292106/1‑110 (MQ=255)
                                                                                              aaCCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGc                                                                                                    <  5:273237/104‑1 (MQ=255)
                                                                                              aaCCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGc                                                                                                    >  6:273237/1‑104 (MQ=255)
                                                                                               aCCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTc                                                                                  >  5:11928/1‑121 (MQ=255)
                                                                                               aCCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTc                                                                                  <  6:11928/121‑1 (MQ=255)
                                                                                                 cGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGctac                                                    <  1:402597/149‑1 (MQ=255)
                                                                                                 cGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGctac                                                    <  6:62051/149‑1 (MQ=255)
                                                                                                         ctgGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCAc                                            >  5:24088/1‑149 (MQ=255)
                                                                                                          tgGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACt                                           >  1:174420/1‑149 (MQ=255)
                                                                                                           ggACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTg                                          >  7:247328/1‑149 (MQ=255)
                                                                                                            gACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTgg                                         <  8:223689/149‑1 (MQ=255)
                                                                                                               gAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGa                                      >  2:276155/1‑149 (MQ=255)
                                                                                                               gAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGa                                      >  4:137093/1‑149 (MQ=255)
                                                                                                                 aaaaGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTa                                                                          >  1:4507/1‑111 (MQ=255)
                                                                                                                 aaaaGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTa                                                                          <  2:4507/111‑1 (MQ=255)
                                                                                                                  aaaGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACgg                                   >  5:403254/1‑149 (MQ=255)
                                                                                                                    aGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTg                                 <  2:174420/149‑1 (MQ=255)
                                                                                                                     ggCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCa                                             >  2:391109/1‑136 (MQ=255)
                                                                                                                     ggCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCa                                             <  1:391109/136‑1 (MQ=255)
                                                                                                                     ggCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGc                                <  6:403254/149‑1 (MQ=255)
                                                                                                                       cAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACCGCGACCACCAGCCGATGGTCGACACCTTCTACAAGCCCCTGCCCCGCAACTACTTCCCTGGCGAcaggcacc                              >  5:122199/1‑142 (MQ=255)
                                                                                                                              aTCGAAGGCCCCGGCGCCGGCGGGCGGGCGAGCATCGCGAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGCTGGGCTACACCTCCTTCACGCCCCTGCCCGGCTACTCCTTCACTGGCGACGGTGCCCGTCGGGa                       <  8:95447/149‑1 (MQ=255)
                                                                                                                               tCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTcg                          >  7:217923/1‑145 (MQ=255)
                                                                                                                               tCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTcg                          <  8:217923/145‑1 (MQ=255)
                                                                                                                               tCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGAt                      >  4:312807/1‑149 (MQ=255)
                                                                                                                               tCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGAt                      <  4:168935/149‑1 (MQ=255)
                                                                                                                                 gAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGc                    >  5:18236/1‑149 (MQ=255)
                                                                                                                                        ccggcgccggcgTGCTGGCGATCAAAGCCAGCTGGCCCGTGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGc             <  1:242406/149‑1 (MQ=255)
                                                                                                                                                   gTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGAt  <  4:161749/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGGCAGACCGAAACCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGAT  >  NC_002947/5345830‑5346125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑