Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,345,981 T→C 7.3% L460P (CTG→CCG)  acsA‑II → acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,345,9810TC 7.3% 194.1 / 4.9 69L460P (CTG→CCG) acsA‑IIacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (32/31);  new base C (2/3);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

CGAAACCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACC  >  NC_002947/5345838‑5346129
                                                                                                                                               |                                                                                                                                                    
cGAAACCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAAACCGGCTGCGCCACCCAGACGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCAGCACGCCGGCgc                                                                                                                                                 >  6:28231/1‑148 (MQ=255)
  aaaCCGGCGGCATCATGCTCACCCCTCTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATc                                                                                                                                               <  1:400319/149‑1 (MQ=255)
  aaaCCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATc                                                                                                                                               <  8:264138/149‑1 (MQ=255)
   aaCCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCAGCACGCCGGCgccgg                                                                                                                                              <  5:28231/149‑5 (MQ=255)
    aCCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCAGCACGCCGGCgccggg                                                                                                                                             <  6:96075/149‑6 (MQ=255)
     ccGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCaaa                                                                                                                                            <  6:97961/149‑1 (MQ=255)
     ccGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCaaa                                                                                                                                            >  3:379469/1‑149 (MQ=255)
     ccGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCaaa                                                                                                                                            >  3:287852/1‑149 (MQ=255)
     ccGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCa                                                                                                                                              >  5:97961/1‑147 (MQ=255)
         cggcATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCa                                                                                                                                        <  2:71385/149‑1 (MQ=255)
            catcatGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCt                                                                                                                                     <  4:287852/149‑1 (MQ=255)
            catcatGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCt                                                                                                                                     >  8:192915/1‑149 (MQ=255)
            catcatGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCt                                                                                                                                     >  5:287787/1‑149 (MQ=255)
                atGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGc                                                                                                                                  <  6:26702/148‑1 (MQ=255)
                      aCCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCaaa                                                                                                                                            >  6:181871/1‑132 (MQ=255)
                      aCCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCaaa                                                                                                                                            <  5:181871/132‑1 (MQ=255)
                      aCCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGc                                                                                                                            <  2:339763/148‑1 (MQ=255)
                          cACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATc                                                                                                                       >  1:367081/1‑149 (MQ=255)
                               cccGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGc                                                                                                                    <  6:287787/147‑1 (MQ=255)
                                ccGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGc                                                                                                                 <  7:192915/149‑1 (MQ=255)
                                  ggCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGt                                                                                                               >  3:161749/1‑149 (MQ=255)
                                    cacacaGTCACTCAAACCCGTCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCt                                                                                                             <  5:410424/149‑1 (MQ=255)
                                             aCTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGac                                                                                                     >  3:261625/1‑148 (MQ=255)
                                               tCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCAGCACGCCGGCgccg                                                                                                                                               >  7:163713/1‑101 (MQ=255)
                                               tCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCAGCACGCCGGCgccg                                                                                                                                               <  8:163713/104‑4 (MQ=255)
                                                 aaaCCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGAccacca                                                                                                >  2:402597/1‑149 (MQ=255)
                                                               cACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGac                                                                                   <  2:182738/148‑1 (MQ=255)
                                                               cACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGac                                                                                   >  1:182738/1‑148 (MQ=255)
                                                                aCCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGacac                                                                                 >  8:11036/1‑149 (MQ=255)
                                                                 cccAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACAcc                                                                                <  7:309857/149‑1 (MQ=255)
                                                                       cGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCGCGGGCAGATCCGCATATTCTACTGCGACGACCAGCGGATGGTCGACACCTACTTc                                                                          <  4:379469/149‑1 (MQ=255)
                                                                              cGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGcccc                                                                   >  7:223689/1‑149 (MQ=255)
                                                                              cGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGcccc                                                                   >  5:62051/1‑149 (MQ=255)
                                                                              cGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTCGCGATCAACGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGcccc                                                                   >  6:413978/1‑149 (MQ=255)
                                                                                gCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGc                                                                                                                                                   <  6:275658/67‑1 (MQ=255)
                                                                                gCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGc                                                                                                                                                   >  5:275658/1‑67 (MQ=255)
                                                                                  gTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTAc                                                                                                           >  1:44961/1‑105 (MQ=255)
                                                                                  gTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTAc                                                                                                           <  2:44961/105‑1 (MQ=255)
                                                                                    gCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGacc                                                                                                    <  4:292106/110‑1 (MQ=255)
                                                                                    gCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGacc                                                                                                    >  3:292106/1‑110 (MQ=255)
                                                                                      aaCCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGc                                                                                                        >  6:273237/1‑104 (MQ=255)
                                                                                      aaCCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGc                                                                                                        <  5:273237/104‑1 (MQ=255)
                                                                                       aCCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTc                                                                                      <  6:11928/121‑1 (MQ=255)
                                                                                       aCCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTc                                                                                      >  5:11928/1‑121 (MQ=255)
                                                                                         cGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGctac                                                        <  6:62051/149‑1 (MQ=255)
                                                                                         cGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGctac                                                        <  1:402597/149‑1 (MQ=255)
                                                                                                 ctgGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCAc                                                >  5:24088/1‑149 (MQ=255)
                                                                                                  tgGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACt                                               >  1:174420/1‑149 (MQ=255)
                                                                                                   ggACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTg                                              >  7:247328/1‑149 (MQ=255)
                                                                                                    gACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTgg                                             <  8:223689/149‑1 (MQ=255)
                                                                                                       gAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGa                                          >  2:276155/1‑149 (MQ=255)
                                                                                                       gAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGa                                          >  4:137093/1‑149 (MQ=255)
                                                                                                         aaaaGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTa                                                                              >  1:4507/1‑111 (MQ=255)
                                                                                                         aaaaGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTa                                                                              <  2:4507/111‑1 (MQ=255)
                                                                                                          aaaGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACgg                                       >  5:403254/1‑149 (MQ=255)
                                                                                                            aGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTg                                     <  2:174420/149‑1 (MQ=255)
                                                                                                             ggCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCa                                                 >  2:391109/1‑136 (MQ=255)
                                                                                                             ggCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCa                                                 <  1:391109/136‑1 (MQ=255)
                                                                                                             ggCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGc                                    <  6:403254/149‑1 (MQ=255)
                                                                                                               cAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACCGCGACCACCAGCCGATGGTCGACACCTTCTACAAGCCCCTGCCCCGCAACTACTTCCCTGGCGAcaggcacc                                  >  5:122199/1‑142 (MQ=255)
                                                                                                                      aTCGAAGGCCCCGGCGCCGGCGGGCGGGCGAGCATCGCGAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGCTGGGCTACACCTCCTTCACGCCCCTGCCCGGCTACTCCTTCACTGGCGACGGTGCCCGTCGGGa                           <  8:95447/149‑1 (MQ=255)
                                                                                                                       tCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTcg                              >  7:217923/1‑145 (MQ=255)
                                                                                                                       tCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTcg                              <  8:217923/145‑1 (MQ=255)
                                                                                                                       tCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGAt                          >  4:312807/1‑149 (MQ=255)
                                                                                                                       tCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGAt                          <  4:168935/149‑1 (MQ=255)
                                                                                                                         gAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGc                        >  5:18236/1‑149 (MQ=255)
                                                                                                                                ccggcgccggcgTGCTGGCGATCAAAGCCAGCTGGCCCGTGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGc                 <  1:242406/149‑1 (MQ=255)
                                                                                                                                         gcttGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCGGCAGCGTCTACGGCGACCACCAGCGGATGGTCGTCACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTgg        <  4:117956/146‑1 (MQ=255)
                                                                                                                                           gTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGAt      <  4:161749/149‑1 (MQ=255)
                                                                                                                                               tGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCAcc  >  1:68371/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CGAAACCGGCGGCATCATGCTCACCCCACTGCCCGGCACACAGTCACTCAAACCCGGCTGCGCCACCCAGCCGATGTTCGGCGTGCAACCGGTGCTGCTGGACGAAAAAGGCAAACTGATCGAAGGCCCCGGCGCCGGCGTGCTGGCGATCAAAGCCAGCTGGCCCGGGCAGATCCGCAGCGTCTACGGCGACCACCAGCGGATGGTCGACACCTACTTCAAGCCCCTGCCCGGCTACTACTTCACTGGCGACGGTGCCCGTCGCGATGCCGACGGCGACTACTGGATCACC  >  NC_002947/5345838‑5346129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑