Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,367,613 G→T 5.1% E45D (GAG→GAT)  PP_4720 → RNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,367,6130GT 5.1% 259.7 / 3.4 78E45D (GAG→GAT) PP_4720RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (30/44);  new base T (2/2);  total (32/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.50e-01

AAAGGGTCAGATTATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGC  >  NC_002947/5367466‑5367761
                                                                                                                                                   |                                                                                                                                                    
aaaGGGTCAGATTATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGc                                                                                                                                                     >  7:97452/1‑149 (MQ=255)
   tgggccgATTATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGa                                                                                                                                                  <  6:291470/143‑1 (MQ=255)
   gggTCAGATTATGCCGCTCAATAACGAGCAGTAGAAGCAATACAAGTCGATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGa                                                                                                                                                  <  1:376394/149‑1 (MQ=255)
      tCAGATTATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATc                                                                                                                                                >  8:272705/1‑148 (MQ=255)
         gATTATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAgg                                                                                                                                            <  3:226719/149‑1 (MQ=255)
          aTTATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGt                                                                                                                                           >  8:331578/1‑149 (MQ=255)
           ttATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCTTGATCAGCTCATGGTc                                                                                                                                          >  8:62253/1‑149 (MQ=255)
              tGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCgag                                                                                                                                       <  5:54636/149‑1 (MQ=255)
              tGCCGCGCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCTTGATCAGCTCATGGTCga                                                                                                                                        <  7:62253/148‑1 (MQ=255)
               gCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACAATGATCTGATCAAGGTc                                                                                                                                          >  5:212198/1‑145 (MQ=255)
                      aaTAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTc                                                                                                                               <  7:71198/149‑1 (MQ=255)
                        tAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAg                                                                                                                                                      <  4:117150/124‑1 (MQ=255)
                        tAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAg                                                                                                                                                      >  3:117150/1‑124 (MQ=255)
                         aaCGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGa                                                                                                                            <  8:97452/149‑1 (MQ=255)
                                agaagaAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACcgc                                                                                                                     >  4:68774/1‑149 (MQ=255)
                                 gaagaaGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACcgcg                                                                                                                    <  1:130560/149‑1 (MQ=255)
                                  aagaagCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACcgcg                                                                                                                    >  5:252651/1‑148 (MQ=255)
                                  aagaagCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACcgcg                                                                                                                    >  1:398666/1‑148 (MQ=255)
                                  aagaagCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCga                                                                                                                   <  5:70505/149‑1 (MQ=255)
                                   agaagCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCgaa                                                                                                                  <  3:68774/149‑1 (MQ=255)
                                    gaagaaATACAAGTCAATTGGTCATGACCTGAAGCAGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATAGCCGAACTGGAGCGCGCTCTGGTCGACAATGAGCTGATCAAGGTAGAGATTCGCTCTGAAGACagcg                                                                                                                    >  6:246997/1‑146 (MQ=255)
                                    gaagCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACcgcg                                                                                                                    >  6:257432/1‑146 (MQ=255)
                                    gaagCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACcgcg                                                                                                                    >  6:241177/1‑146 (MQ=255)
                                    ctagCAATACAAGTCCATTGGTCTTGACCTGAAGCCTGTCCTGATCGTTGCTGGCAACGTTTTGAATGAAGGCGTGATCGACGGACTGGAGCGCGCTCTGTTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCgaa                                                                                                                  <  6:267508/146‑1 (MQ=255)
                                     aagCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAAGTCGAGATTCGCTCGGAAGACCGCgaag                                                                                                                 >  8:219393/1‑148 (MQ=255)
                                      agCAATACAAGTCCATTGGTCATGACCTGATGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCgaagaa                                                                                                               >  1:38024/1‑149 (MQ=255)
                                       gCAATAGAAGTCCATTGGTCATGAGCTGAAGCCGGTCCTGATCGTTGCTGGCAAGGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAAc                                                                                                              <  6:212464/149‑1 (MQ=255)
                                       gCAATACAAGTCAATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGAGTCATCGCAGGACTGGAGCGCGCTCTGGTCGACCATTAGCTGATCAAGGTCGAGATTCGCTCGGAAGACAGCgaagag                                                                                                               >  5:267508/1‑147 (MQ=255)
                                          aTACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAAcgcg                                                                                                           <  2:299380/149‑1 (MQ=255)
                                           tACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACgcgc                                                                                                          >  4:266419/1‑149 (MQ=255)
                                            aCAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGcc                                                                                                         >  8:195262/1‑149 (MQ=255)
                                            aCAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGAAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGcc                                                                                                         >  4:255769/1‑149 (MQ=255)
                                             cAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCaa                                                                                                                                              <  5:419570/111‑1 (MQ=255)
                                             cAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCaa                                                                                                                                              >  6:419570/1‑111 (MQ=255)
                                              aaGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTTGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCga                                                                                                       <  2:20377/149‑1 (MQ=255)
                                                 tCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGAc                                                                                                    <  7:272705/149‑1 (MQ=255)
                                                   cATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGAcc                                                                                                   <  7:195262/148‑1 (MQ=255)
                                                   cATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCa                                                                                                  >  4:73487/1‑149 (MQ=255)
                                                    aTTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGAcaa                                                                                                  >  2:46194/1‑146 (MQ=255)
                                                     ttGGTCATGACCTGAAGCCTGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAAGTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATc                                                                                                <  6:369721/149‑1 (MQ=255)
                                                           aTGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAg                                                                                          <  4:345794/149‑1 (MQ=255)
                                                               ccTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGATCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCtgt                                                                                      <  6:212198/149‑1 (MQ=255)
                                                                  gAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCt                                                                                        <  7:211943/144‑1 (MQ=255)
                                                                  gAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCt                                                                                        >  8:211943/1‑144 (MQ=255)
                                                                     gCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAAGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAgg                                                                                <  7:219393/149‑1 (MQ=255)
                                                                            cTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGgccggccg                                                                         >  4:189674/1‑149 (MQ=255)
                                                                               aTCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGt                                                                        <  2:38024/147‑1 (MQ=255)
                                                                               aTCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTg                                                                       <  7:331578/148‑1 (MQ=255)
                                                                               aTCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGc                                                                      >  1:166361/1‑149 (MQ=255)
                                                                                   ttGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAg                                                                  >  6:345834/1‑149 (MQ=255)
                                                                                       tGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTgg                                                              >  2:148956/1‑149 (MQ=255)
                                                                                        ggCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCga                                                                                                                   >  3:304472/1‑95 (MQ=255)
                                                                                        ggCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCga                                                                                                                   <  4:304472/95‑1 (MQ=255)
                                                                                         gCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCGGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTg                                                            <  6:252651/149‑1 (MQ=255)
                                                                                         gCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTg                                                            <  1:148956/149‑1 (MQ=255)
                                                                                         gCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTg                                                            <  1:46194/149‑1 (MQ=255)
                                                                                         gCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTg                                                            <  2:398666/149‑1 (MQ=255)
                                                                                          cAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGTCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGc                                                           <  3:387684/149‑1 (MQ=255)
                                                                                           aaCGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCa                                                          <  5:257432/149‑1 (MQ=255)
                                                                                                    catgcacgCGTGATCGCCGAACTGGCGCGCGGTCGGGGCGTCCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCgg                                                 <  3:31896/142‑1 (MQ=255)
                                                                                                    aatgaaGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCgg                                                 <  3:73487/149‑1 (MQ=255)
                                                                                                     atgaaGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCgg                                                 >  1:45600/1‑148 (MQ=255)
                                                                                                      tgaaGGCGGGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCa                                               <  8:41452/149‑1 (MQ=255)
                                                                                                        aaGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTg                                                               <  7:107721/131‑1 (MQ=255)
                                                                                                        aaGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTg                                                               >  8:107721/1‑131 (MQ=255)
                                                                                                                   gCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGa                                   <  3:189674/148‑1 (MQ=255)
                                                                                                                        aCTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTAc                              <  2:166361/148‑1 (MQ=255)
                                                                                                                            gAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCtgt                                                                                      <  6:332489/88‑1 (MQ=255)
                                                                                                                            gAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCtgt                                                                                      >  5:332489/1‑88 (MQ=255)
                                                                                                                            gAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCaa                                              <  8:353236/128‑1 (MQ=255)
                                                                                                                            gAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCaa                                              >  7:353236/1‑128 (MQ=255)
                                                                                                                                 cgcTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTg                                                            <  3:304384/109‑1 (MQ=255)
                                                                                                                                 cgcTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTg                                                            >  4:304384/1‑109 (MQ=255)
                                                                                                                                   ctctGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCca                  <  5:345834/149‑1 (MQ=255)
                                                                                                                                            aCCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAAc         <  5:241177/149‑1 (MQ=255)
                                                                                                                                            aCCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAAc         <  3:255769/149‑1 (MQ=255)
                                                                                                                                             ccATGAGCTGATCAAGGTCGAGATTCTCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGACGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAAc         <  5:246997/148‑1 (MQ=255)
                                                                                                                                                  aGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCg                                                  >  6:360861/1‑102 (MQ=255)
                                                                                                                                                  aGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCg                                                  <  5:360861/102‑1 (MQ=255)
                                                                                                                                                  aGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAagcag   <  3:266419/149‑1 (MQ=255)
                                                                                                                                                   gCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAagcagc  >  7:28822/1‑149 (MQ=255)
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AAAGGGTCAGATTATGCCGCTCAATAACGAGCAGAAGAAGCAATACAAGTCCATTGGTCATGACCTGAAGCCGGTCCTGATCGTTGCTGGCAACGGTTTGAATGAAGGCGTGATCGCCGAACTGGAGCGCGCTCTGGTCGACCATGAGCTGATCAAGGTCGAGATTCGCTCGGAAGACCGCGAAGAACGCGCCGAGACCATCGCCGAGCTGTGCAAGGCCGGCCGTGCCGAGCTGGTGCAGACCATCGGCAAGAAAGCCCTGATCTACCGCAAGAACCCACAGCCGAACAAGCAGC  >  NC_002947/5367466‑5367761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑