Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,388,882 C→A 5.0% L798M (CTG→ATG)  dld2 → D‑lactate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,388,8820CA 5.0% 265.7 / 3.6 83L798M (CTG→ATG) dld2D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (33/45);  new base A (2/2);  total (36/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

GCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCGAAAG  >  NC_002947/5388736‑5389030
                                                                                                                                                  |                                                                                                                                                     
tccTGTGATGCTGCCAACCTTTCGCCTACAAGGGATACCCCTAGCAGTCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGTCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑g                                                                                                                                                    <  8:377702/148‑1 (MQ=255)
gCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑g                                                                                                                                                    <  1:207997/149‑1 (MQ=255)
       ctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCGCCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAg                                                                                                                                              <  6:48338/148‑1 (MQ=255)
       ctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAgg                                                                                                                                             <  8:148616/149‑1 (MQ=255)
       ctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAg                                                                                                                                              <  7:390265/148‑1 (MQ=255)
             ccAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCTT‑GGGGAAGGAcgtcg                                                                                                                                       >  3:180609/1‑145 (MQ=255)
             ccAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGa                                                                                                                                       <  6:381849/149‑1 (MQ=255)
               aaCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGc                                                                                                                                     <  2:2916/149‑1 (MQ=255)
                acagTTCGCCTCCAAGGGATACCCCGAGCAGGCCGAACACAAACGCAAGGAGCTGATAACCGACCTGCTGCAAGCCAGCCGCTGCGGCCTCGACCCGATCTATTGCGACACAATCACATGCACCCTGCCCCT‑GGTGCACGACCTGa                                                                                                                                       >  6:41570/4‑146 (MQ=255)
                       gCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCg                                                                                                                             >  6:11531/1‑149 (MQ=255)
                        ccTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCgg                                                                                                                            <  3:227472/149‑1 (MQ=255)
                           ccAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                         >  1:75488/1‑149 (MQ=255)
                            cAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCTTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                          <  8:146392/147‑1 (MQ=255)
                            cAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                         >  2:208147/1‑148 (MQ=255)
                            cAAGGGCTACCCCGAGCAGGCCAAACACAAACGCCAGGAGCTTATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                         <  8:170212/148‑1 (MQ=255)
                             aaGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                       >  1:347317/1‑149 (MQ=255)
                              aGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                      <  5:11531/149‑1 (MQ=255)
                              aGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                      <  8:133157/149‑1 (MQ=255)
                               gggCTACCCCGATCAGGCCGAACACAATCGCCAGGAGATGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCTACACCCGGCTGGAcc                                                                                                                     <  5:41570/149‑1 (MQ=255)
                               gggCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGAcc                                                                                                                     >  2:132137/1‑149 (MQ=255)
                                  cTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGt                                                                                                                  <  3:41843/149‑1 (MQ=255)
                                      cccGAGCAGGCCGAACACAAACGACAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGa                                                                                                              >  7:62957/1‑149 (MQ=255)
                                        cGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGACTCGACCAGATCTATTGCGACACAAGCCACTGCACCCTGCGACT‑GGTGCAGGACCTGCGCGACACCCGGCTTGACCTGTACGAcc                                                                                                            >  4:249270/1‑149 (MQ=255)
                                            cAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCTGGCTGGACCTGTACGACCCCGt                                                                                                        <  1:87643/149‑1 (MQ=255)
                                            cAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGt                                                                                                        <  2:75488/149‑1 (MQ=255)
                                             aGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGc                                                                                                                                     >  8:13335/1‑119 (MQ=255)
                                             aGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGc                                                                                                                                     <  7:13335/119‑1 (MQ=255)
                                             aGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTg                                                                                                       <  6:181771/149‑1 (MQ=255)
                                                cctaaCACAAACGCAAGGAGCTTATCACCGCCCTGCTGCACGCCAGCCGCGTCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCTACACCCGGCTGGACCTGTACGACCCCGTgcgc                                                                                                    <  3:249270/146‑1 (MQ=255)
                                                 cGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCt                                                                                                   <  1:350548/149‑1 (MQ=255)
                                                 cGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAG‑GGTGCAggggctt                                                                                                                                        <  2:378854/112‑6 (MQ=38)
                                                 cGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAG‑GGTGCAggggctt                                                                                                                                        >  1:378854/1‑107 (MQ=38)
                                                  gAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCtt                                                                                                  <  2:347317/149‑1 (MQ=255)
                                                    acacAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcgc                                                                                                    <  5:197182/145‑1 (MQ=255)
                                                    acacAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcgc                                                                                                    >  6:197182/1‑145 (MQ=255)
                                                        aaaCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCg                                                                                            >  4:363648/1‑149 (MQ=255)
                                                        aaaCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCg                                                                                            >  8:88474/1‑149 (MQ=255)
                                                         aaCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGc                                                                                           <  1:284839/149‑1 (MQ=255)
                                                          aCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGca                                                                                          <  1:208147/149‑1 (MQ=255)
                                                          aCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGca                                                                                          <  4:263110/149‑1 (MQ=255)
                                                                 gAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                                   >  3:103204/1‑149 (MQ=255)
                                                                       aTCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                      >  3:357330/1‑108 (MQ=255)
                                                                       aTCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                      <  4:357330/108‑1 (MQ=255)
                                                                        tCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgac                                                                            >  7:34010/1‑149 (MQ=255)
                                                                           ccGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTg                                                                                                       >  1:363484/1‑119 (MQ=255)
                                                                           ccGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTg                                                                                                       <  2:363484/119‑1 (MQ=255)
                                                                                  gctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTg                                                                      <  2:143445/145‑1 (MQ=255)
                                                                                  gctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTg                                                                      >  1:223671/1‑145 (MQ=255)
                                                                                  gctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTg                                                                      <  2:223671/145‑1 (MQ=255)
                                                                                  gctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTg                                                                      >  1:143445/1‑145 (MQ=255)
                                                                                   ctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGtt                                                                 <  4:103204/149‑1 (MQ=255)
                                                                                     gcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                               >  3:311445/1‑149 (MQ=255)
                                                                                       aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcac                                                                                         >  5:398423/1‑121 (MQ=255)
                                                                                       aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcac                                                                                         <  6:398423/121‑1 (MQ=255)
                                                                                               cgcgGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGa                                                     <  7:88474/149‑1 (MQ=255)
                                                                                                gcggcgGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGAc                                                    <  8:62957/149‑1 (MQ=255)
                                                                                                gcggcgGCCTCGACCCGATCAATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGAc                                                    >  8:122741/1‑149 (MQ=255)
                                                                                                     ggcCTCGACCCGATCTATTTCGACAAAACCCCCTTTACCCTGCGCCTAGCATCTGGACCTGAGCGACACCCCGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAc                                                <  1:132137/149‑1 (MQ=255)
                                                                                                        cTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGTAGTTCACCCCCCAGGACGAACCGGt                                            <  3:363648/149‑1 (MQ=255)
                                                                                                        cTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                               >  1:159045/1‑130 (MQ=255)
                                                                                                        cTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                               >  1:153386/1‑130 (MQ=255)
                                                                                                        cTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                               <  2:153386/130‑1 (MQ=255)
                                                                                                        cTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                               <  2:159045/130‑1 (MQ=255)
                                                                                                           gACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGCCGACCCGGtcga                                         >  8:124316/1‑146 (MQ=255)
                                                                                                             cccGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGa                                                  <  2:16381/138‑1 (MQ=255)
                                                                                                             cccGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGa                                                  >  1:16381/1‑138 (MQ=255)
                                                                                                                gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgtg                                     >  4:126546/1‑148 (MQ=255)
                                                                                                                    tATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCTCCCACCTGCTCGACCGACTGGAGTTCACCCCCCa                                                        <  1:167632/125‑1 (MQ=255)
                                                                                                                    tATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCa                                                        >  2:167632/1‑125 (MQ=255)
                                                                                                                     aTTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCgg                                             >  1:382296/1‑135 (MQ=255)
                                                                                                                     aTTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCgg                                             <  2:382296/135‑1 (MQ=255)
                                                                                                                      ttGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCAGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCt                                                                                >  3:214623/1‑99 (MQ=255)
                                                                                                                      ttGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCAGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCt                                                                                <  4:214623/99‑1 (MQ=255)
                                                                                                                        gCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGAc                             <  3:126546/148‑1 (MQ=255)
                                                                                                                            cacaAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTg                          >  6:309750/1‑147 (MQ=255)
                                                                                                                             acaAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcag                       >  1:266600/1‑149 (MQ=255)
                                                                                                                              tcaggtccTGCACCAGGCGCAG‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGa                              >  4:386653/7‑141 (MQ=255)
                                                                                                                              tcaggtccTGCACCAGGCGCAG‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGa                              <  3:386653/135‑1 (MQ=255)
                                                                                                                               aaGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcatca                     >  8:299739/1‑149 (MQ=255)
                                                                                                                                 gCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAc                                                <  5:160064/120‑1 (MQ=255)
                                                                                                                                 gCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAc                                                >  6:160064/1‑120 (MQ=255)
                                                                                                                                  ccccTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcaccc                  <  7:299739/149‑1 (MQ=255)
                                                                                                                                            cTGCGCCT‑GGTGCAGGACCTGTGCGACACCCGGCTGGACCTGTACGCCCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTggg        <  2:90671/149‑1 (MQ=255)
                                                                                                                                                 ccT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCGaaa   <  5:309750/149‑1 (MQ=255)
                                                                                                                                                 ccT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCGa     >  8:18046/1‑147 (MQ=255)
                                                                                                                                                  cT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCGAAAg  >  7:49841/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCT‑GGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCGAAAG  >  NC_002947/5388736‑5389030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑