Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,390,656	G→C	5.6%	noncoding (24/87 nt)	PP_mr56 →	C4 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,390,656	0	G	C	5.6%	222.7 / 3.6	72	noncoding (24/87 nt)	PP_mr56	C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base G (35/32);  new base C (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

TTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTCACCACCTC  >  NC_002947/5390509‑5390804                                                                                                                                                     |                                                                                                                                                     ttCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGAGCGCATAACCGTTCATGGCGGTTGTGCATgggg                                                                                                                                                     >  5:95498/1‑149 (MQ=255)  tCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATgggg                                                                                                                                                     <  3:187227/148‑1 (MQ=255)     cAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACg                                                                                                                                                 >  5:315404/1‑149 (MQ=255)      aaaGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGc                                                                                                                                                >  7:13908/1‑149 (MQ=255)            aCTGCCTGCGCCTGTTTTCCGTCGCGGTCA‑TTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTtgcgtg                                                                                                                                         >  7:62902/1‑149 (MQ=255)              tgcctgcGCCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGCCGGTTGTGCATGGGGCACGCTtgcgtgc                                                                                                                                        >  8:334523/1‑149 (MQ=255)              tgcctgcGCCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACAGTTCATGGCGGTTGTGCATGGGGCACGCTtgcgtgc                                                                                                                                        >  4:33514/1‑149 (MQ=255)                cctgcGCCTGTTTTCCGTTGCGGTCATTTTTTGCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTtgcgtgcg                                                                                                                                       >  1:256081/1‑149 (MQ=255)                cctgcGCCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTgcgc                                                                                                                                      >  4:260913/1‑149 (MQ=255)                    cgcCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCg                                                                                                                                    <  6:347708/147‑1 (MQ=255)                    cgcCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACGCAagcgtacccca                                                                                                                                    <  6:315404/147‑7 (MQ=255)                        tGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                                                                                              >  3:39912/1‑149 (MQ=255)                         gTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCtg                                                                                                                             >  3:213812/1‑149 (MQ=255)                         gTTTTCCGTTGCGGTCA‑TTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCtgt                                                                                                                            <  8:62902/149‑1 (MQ=255)                          ttttCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                                                                                              <  1:6130/147‑1 (MQ=255)                            ttCCGTTGCGGTCATTTTTT‑GAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCtgtg                                                                                                                           <  4:53337/148‑1 (MQ=255)                                gTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGc                                                                                                                      <  4:213812/149‑1 (MQ=255)                                      gTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGAATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCt                                                                                                                >  5:300121/1‑149 (MQ=255)                                        cATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGt                                                                                                              >  2:227379/1‑149 (MQ=255)                                         aTTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACGCAAGCGTGcccca                                                                                                                                    >  8:230014/1‑125 (MQ=39)                                         aTTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACGCAAGCGTGcccca                                                                                                                                    <  7:230014/126‑2 (MQ=39)                                             ttt‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAAc                                                                                                          <  5:366591/148‑1 (MQ=255)                                               t‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAAccc                                                                                                        <  5:300800/148‑1 (MQ=255)                                                  aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAAc                                                                                                          <  8:280972/144‑1 (MQ=255)                                                  aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAAc                                                                                                          >  7:280972/1‑144 (MQ=255)                                                  aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCAt                                                                                                      >  7:122828/1‑148 (MQ=255)                                                  aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCAt                                                                                                      <  8:122828/148‑1 (MQ=255)                                                  aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCAt                                                                                                      <  4:39912/148‑1 (MQ=255)                                                  aGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATg                                                                                                     >  5:265084/1‑149 (MQ=255)                                                    ggTTTTAGTCTAGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATAAACAGCGCGCATAACCGTTCATGGAGGTTGTGCATGGGGCACGCTTGCTTGCGCCGGTTTCTGTGTTCGAAGGTCTGTCAACCCATGca                                                                                                   >  2:393292/1‑149 (MQ=255)                                                      ttttAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGca                                                                                                   >  3:397667/1‑147 (MQ=255)                                                                  tGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCAtt                                                                                     <  6:265084/149‑1 (MQ=255)                                                                  tGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTACCACCAtt                                                                                     <  7:334523/149‑1 (MQ=255)                                                                    ggTCGTTGATGAGCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTcc                                                                                   >  4:130236/1‑149 (MQ=255)                                                                      tCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGt                                                                                 <  3:33514/149‑1 (MQ=255)                                                                      tCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGt                                                                                 >  6:169564/1‑149 (MQ=255)                                                                        gTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGttt                                                                               <  2:256081/149‑1 (MQ=255)                                                                            cTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTc                                                                                    <  4:51770/140‑1 (MQ=255)                                                                            cTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTc                                                                                    >  3:51770/1‑140 (MQ=255)                                                                             tcatcaACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAg                                                                          >  6:403933/1‑149 (MQ=255)                                                                               atcaACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCg                                                                        >  3:160757/1‑149 (MQ=255)                                                                               atcaACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCg                                                                        <  8:13908/149‑1 (MQ=255)                                                                                 caACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCggg                                                                      >  2:85295/1‑149 (MQ=255)                                                                                       ccGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGATCAAGGCGGTTCTGCATGCGGCACGCTTGCCTGCGCCGGTTTCTCTGTTCGCCGGACTGTCAACACATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCg                                                                >  3:205978/1‑149 (MQ=255)                                                                                        cGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAAc                                                                                                          <  1:58152/106‑1 (MQ=255)                                                                                        cGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAAc                                                                                                          >  2:58152/1‑106 (MQ=255)                                                                                          gCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTg                                                             >  4:267335/1‑149 (MQ=255)                                                                                           cTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTgg                                                            <  1:235892/149‑1 (MQ=255)                                                                                            tttGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCAGGTTTCTGTGTTCGACGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGTCGGTTGc                                                           <  1:393292/149‑1 (MQ=255)                                                                                               taCAGGCGGCAACGATACACAGCGGTCATTACCGTTCATGTCGGTTGTTAATGGTGGACGCTTGGGTGCTCCGGTTTCTGTGTTCGCCGGTCTGTCAAGCCATGGACAGCTGCCACCATTCCGTTTGACAGCTGGTTGGCGGTggggc                                                         <  4:205978/147‑4 (MQ=255)                                                                                               gACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGAGGTGGcccc                                                        >  8:259108/1‑149 (MQ=255)                                                                                                aCAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGcccc                                                        <  7:164454/148‑1 (MQ=255)                                                                                                   ggCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATc                                                    <  1:85295/149‑1 (MQ=255)                                                                                                     ccGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCga                                                  <  5:403933/149‑1 (MQ=255)                                                                                                      cGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCgag                                                 <  3:267335/149‑1 (MQ=255)                                                                                                            gATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAAcaca                                           <  6:300121/149‑1 (MQ=255)                                                                                                                cacaGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTaa                                       <  3:260913/149‑1 (MQ=255)                                                                                                                 acaGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTaa                                       >  7:12025/1‑148 (MQ=255)                                                                                                                     cgcgcATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGccc                                                         <  4:86279/126‑1 (MQ=255)                                                                                                                     cgcgcATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGccc                                                         >  3:86279/1‑126 (MQ=255)                                                                                                                     cgcgcATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAg                                          <  6:300679/141‑1 (MQ=255)                                                                                                                     cgcgcATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAg                                          >  5:300679/1‑141 (MQ=255)                                                                                                                        gcATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTa                                >  7:308408/1‑148 (MQ=255)                                                                                                                           tAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGTTTTCTGTGTTCGCCGGTCTGTAAACACATGGACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGTCCCCTATCGAGAACACAGTAAAATGCTAAAGa                            <  5:341419/149‑1 (MQ=255)                                                                                                                           tAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGa                            <  4:397667/149‑1 (MQ=255)                                                                                                                            aaCCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTa                                >  1:280536/1‑144 (MQ=255)                                                                                                                              ccGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCg                         >  6:122378/1‑149 (MQ=255)                                                                                                                              ccGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGCTTTCTGTGTTCTCAGGTCAGTCAACACATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCg                         >  5:288836/1‑149 (MQ=255)                                                                                                                               cGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGt                        <  5:122378/149‑1 (MQ=255)                                                                                                                                 ttCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTc                      <  5:169564/149‑1 (MQ=255)                                                                                                                                      ggCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGAc                 >  7:13325/1‑149 (MQ=255)                                                                                                                                           ttGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGcc            >  3:52288/1‑149 (MQ=255)                                                                                                                                            agaaaccggggCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCt           >  5:77997/8‑149 (MQ=255)                                                                                                                                                 ccggggCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTcacca      <  6:77997/147‑1 (MQ=255)                                                                                                                                                 aTGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTcacca      >  7:61694/1‑149 (MQ=255)                                                                                                                                                  tGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTcaccac     <  6:95498/149‑1 (MQ=255)                                                                                                                                                   ggggCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTgg                                                            >  7:263632/1‑93 (MQ=255)                                                                                                                                                   ggggCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTgg                                                            <  8:263632/93‑1 (MQ=255)                                                                                                                                                    gggCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTCaccacc    <  8:13325/148‑1 (MQ=255)                                                                                                                                                     ggCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTCACCAtctc  <  4:52288/149‑1 (MQ=255)                                                                                                                                                     ggCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTCACCACCTc  >  3:119262/1‑149 (MQ=255)                                                                                                                                                     ggCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCAGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGAGCGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGAACCTTCCTGACCCGCCTCACAACCt   >  3:254529/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTT‑CAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGATACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTCACCACCTC  >  NC_002947/5390509‑5390804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑