Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,516,304 A→G 5.0% I39V (ATC→GTC)  PP_4849 → molecular chaperone DnaK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,516,3040AG 5.0% 238.6 / 2.4 79I39V (ATC→GTC) PP_4849molecular chaperone DnaK
Reads supporting (aligned to +/- strand):  ref base A (39/36);  new base G (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

CGGCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAG  >  NC_002947/5516159‑5516447
                                                                                                                                                 |                                                                                                                                               
cgGCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCa                                                                                                                                              <  8:35371/149‑1 (MQ=255)
    cGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCtt                                                                                                                                          <  3:17963/149‑1 (MQ=255)
          gCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCt                                                                                                                                     <  2:40876/148‑1 (MQ=255)
          gCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGACCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCt                                                                                                                                     <  5:111510/148‑1 (MQ=255)
           ccTCCGGAAGCCCATTTCCCATGTCTGGCGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCg                                                                                                                                   <  2:229175/149‑1 (MQ=255)
                    gCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttc                                                                                                                          >  5:303046/1‑149 (MQ=255)
                     cccATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttct                                                                                                                         >  3:70176/1‑149 (MQ=255)
                      ccATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                        <  5:128544/149‑1 (MQ=255)
                        aTTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttcttc                                                                                                                       >  5:144405/1‑148 (MQ=255)
                         tttCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCaa                                                                                                                     <  5:256070/149‑1 (MQ=255)
                                tGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGaa                                                                                                              <  2:114369/149‑1 (MQ=255)
                                 gTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCg                                                                                                                >  7:7202/1‑146 (MQ=255)
                                    tGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCtt                                                                                                                                          <  3:236831/117‑1 (MQ=255)
                                    tGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCtt                                                                                                                                          >  4:236831/1‑117 (MQ=255)
                                      aCGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAgcg                                                                                                         <  3:349501/148‑1 (MQ=255)
                                       cGTCTCCCCGGCCCGCGCCCTGGGCAGCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCGATATTGAagaacacc                                                                                                       <  8:163048/149‑6 (MQ=255)
                                         tATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtg                                                                                                                                >  8:113478/1‑122 (MQ=255)
                                         tATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtg                                                                                                                                <  7:113478/122‑1 (MQ=255)
                                           tCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtgg                                                                                                                               <  5:363430/121‑1 (MQ=255)
                                           tCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCggtgg                                                                                                                               >  6:363430/1‑121 (MQ=255)
                                           tCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGccc                                                                                                   >  3:402189/1‑149 (MQ=255)
                                             cccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCgg                                                                                                 >  7:385340/1‑149 (MQ=255)
                                             cccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCg                                                                                                  <  4:402189/148‑1 (MQ=255)
                                              ccGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgt                                                                                                <  1:45079/149‑1 (MQ=255)
                                                  cccGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTAt                                                                                            <  8:385340/149‑1 (MQ=255)
                                                   ccGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATg                                                                                           >  8:214546/1‑149 (MQ=255)
                                                          cTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATgg                                                                                          >  3:278595/1‑143 (MQ=255)
                                                          cTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATgg                                                                                          <  4:278595/143‑1 (MQ=255)
                                                                aTCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCt                                                                              >  5:175630/1‑149 (MQ=255)
                                                                  cGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                        >  7:343969/1‑105 (MQ=255)
                                                                  cGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGttctt                                                                                                                        <  8:343969/105‑1 (MQ=255)
                                                                   gACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCtac                                                                            <  6:144405/148‑3 (MQ=255)
                                                                    aCTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTATTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCAc                                                                          >  8:154863/1‑149 (MQ=255)
                                                                        tGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGa                                                                        <  6:175630/147‑1 (MQ=255)
                                                                          gCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAgcggcg                                                                                                      >  3:82160/1‑115 (MQ=255)
                                                                          gCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAgcggcg                                                                                                      <  4:82160/115‑1 (MQ=255)
                                                                           cACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTAcc                                                                   <  7:214546/149‑1 (MQ=255)
                                                                            aCCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCt                                                                  <  2:376134/149‑1 (MQ=255)
                                                                               tCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGGTGATCTTGCCGTctt                                                                                                                                  >  6:252465/1‑80 (MQ=38)
                                                                               tCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGGTGATCTTGCCGTctt                                                                                                                                  <  5:252465/82‑3 (MQ=38)
                                                                               tCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGGTGATCTTGCCGTct                                                                                                                                   >  2:102850/1‑80 (MQ=255)
                                                                               tCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGGTGATCTTGCCGTct                                                                                                                                   <  1:102850/81‑2 (MQ=255)
                                                                                 cAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAg                                                             >  7:289393/1‑149 (MQ=255)
                                                                                       cacaGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTAc                                                        >  4:233043/1‑148 (MQ=255)
                                                                                        acaGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACg                                                       >  6:145582/1‑148 (MQ=255)
                                                                                          aGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTTTGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAg                                                    >  5:357382/1‑149 (MQ=255)
                                                                                          aGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAg                                                    >  4:216617/1‑149 (MQ=255)
                                                                                                ttGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGgccgcc                                              <  6:303046/149‑1 (MQ=255)
                                                                                                 tGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCt                                             >  1:204426/1‑149 (MQ=255)
                                                                                                    cATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGAt                                          <  5:145582/149‑1 (MQ=255)
                                                                                                        ggCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGGTACGAAGGCCGCCTGATgcgc                                      >  1:219667/1‑149 (MQ=255)
                                                                                                        ggCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGAACCTGGAAGGCTACGAAGGCCGCCTGATgcgc                                      >  4:54450/1‑149 (MQ=255)
                                                                                                            tGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGcgctcgc                                  >  5:884/1‑149 (MQ=255)
                                                                                                              gCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGAACCTGGAAGGCTACGAAGGCCGCCTGATGcgctcgc                                  <  3:54450/147‑1 (MQ=255)
                                                                                                                   gAGTCGCTGATTGCCCTGGAAGGCGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAg                           >  7:62552/1‑149 (MQ=255)
                                                                                                                           gATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTg                                <  6:195653/136‑1 (MQ=255)
                                                                                                                           gATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTg                                >  5:195653/1‑136 (MQ=255)
                                                                                                                            aTTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTggg                  <  4:70176/149‑1 (MQ=255)
                                                                                                                               gCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTc               >  8:382549/1‑149 (MQ=255)
                                                                                                                                  cTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCg              >  2:367017/1‑147 (MQ=255)
                                                                                                                                   tGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACTAGTACCTGGAAGGCTAc                                                        <  7:141233/104‑1 (MQ=255)
                                                                                                                                   tGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTAc                                                        >  8:141233/1‑104 (MQ=255)
                                                                                                                                   tGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCg              >  4:363892/1‑146 (MQ=255)
                                                                                                                                    ggAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGc          >  6:385711/1‑149 (MQ=255)
                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGgcc                                                 <  1:46843/109‑1 (MQ=255)
                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGgcc                                                 >  2:46843/1‑109 (MQ=255)
                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGaa            <  3:363892/146‑1 (MQ=255)
                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAg           <  2:204426/147‑1 (MQ=255)
                                                                                                                                     gAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGc          <  3:233043/148‑1 (MQ=255)
                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgt                                                                                                >  6:15669/1‑56 (MQ=255)
                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGgt                                                                                                <  5:15669/56‑1 (MQ=255)
                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGcgct                                     <  8:133250/115‑1 (MQ=255)
                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGcgct                                     >  7:133250/1‑115 (MQ=255)
                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATc     >  6:197882/1‑147 (MQ=255)
                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCaa   >  6:145687/1‑149 (MQ=255)
                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCaa   >  2:272688/1‑149 (MQ=255)
                                                                                                                                           ggCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGACTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCaa   <  8:7202/149‑1 (MQ=255)
                                                                                                                                             cAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAg  >  1:359238/1‑148 (MQ=255)
                                                                                                                                             cAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAg  >  5:362625/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CGGCCGTTTTGCCTCCGGAAGCCCATTTCCCATGTCTGACGTATCCCCGGCCCGCGCCCTGGGCATCGACTTTGGCACCTCCAACTCCACAGTCGGTTGGCATCGGCCTGGCGTCGAGTCGCTGATTGCCCTGGAAGACGGCAAGATCACCTTGCCCTCGGTGGTGTTCTTCAATATCGAAGAGCGGCGCCCGGTGTATGGCCGCCTGGCGCTGCACGAGTACCTGGAAGGCTACGAAGGCCGCCTGATGCGCTCGCTGAAAAGCCTGCTGGGCTCGAAGCTGATCAAG  >  NC_002947/5516159‑5516447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑