Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,537,067 A→G 11.0% intergenic (+44/+59) nadE → / ← PP_4870 NH(3)‑dependent NAD(+) synthetase/azurin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,537,0670AG11.0% 134.6 / 6.5 54intergenic (+44/+59)nadE/PP_4870NH(3)‑dependent NAD(+) synthetase/azurin
Reads supporting (aligned to +/- strand):  ref base A (19/29);  new base G (3/3);  total (22/32)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTT  >  NC_002947/5536924‑5537212
                                                                                                                                               |                                                                                                                                                 
cTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTGACCCCCGCAAAAGAGGGTGGAcacc                                                                                                                                              >  3:208935/1‑146 (MQ=255)
    cTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGa                                                                                                                                          <  3:230391/149‑1 (MQ=255)
       cATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCCCCGAcc                                                                                                                                        >  6:141257/1‑147 (MQ=255)
           ggCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCAACCGCGAATACGAGGGTGGAACCCCCgccccaa                                                                                                                                     >  1:220605/1‑141 (MQ=255)
               gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCATCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                               <  2:100010/149‑1 (MQ=255)
               gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                               <  5:304241/149‑1 (MQ=25)
               gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                               <  7:81955/149‑1 (MQ=25)
                  ggTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                               >  8:379798/1‑146 (MQ=25)
                  ggTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                               <  7:379798/146‑1 (MQ=25)
                      gACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAAc                                                                                                                         <  2:388480/148‑1 (MQ=255)
                            gCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCggg                                                                                                                              <  5:251879/137‑1 (MQ=255)
                            gCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCggg                                                                                                                              >  6:251879/1‑137 (MQ=255)
                            gCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCCAATAAGAAGGTGAACCCACCCCCCCAATAGCGGGTGAACCCGCGcc                                                                                                                  >  2:3150/1‑149 (MQ=255)
                                  ttCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCacg                                                                                                            >  2:55978/1‑147 (MQ=255)
                                        atcatcGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATAAGAGGGTGAACCCACCGACCCATTAGCGGGTGAACCCGCGCCTGAAGGGGTCGc                                                                                                      >  5:143452/1‑149 (MQ=255)
                                              gtcgCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGGCCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCa                                                                                                 <  7:120207/148‑1 (MQ=255)
                                                     ccTACCGCAACACCACCCAAACGCGCTAACGGCGGTTCGGTCTTTTAAACGGGGCCTCACGGTGGGGTCTCCCCCGCTAAAACGTGGTTCAACCCCCCCACGCTTGCGCGGGTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGa                                                                                         <  4:208935/149‑1 (MQ=255)
                                                        cccGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTc                                                                                                                   <  3:238068/119‑1 (MQ=255)
                                                           gCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAGTACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                               <  5:204248/105‑1 (MQ=255)
                                                           gCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAGTACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                               >  6:204248/1‑105 (MQ=255)
                                                                 cccACCACACGCGCCAACTGCCGTTCGCTCCTTGATAAGGGGCCGGCAGGCGCGGGTTCACCCGCTAATACGCGGGTCAACCCACCGCCGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGa                                                                                         <  8:65886/137‑1 (MQ=37)
                                                                         aaGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAAc                                                                                                                         <  2:266571/97‑1 (MQ=255)
                                                                         aaGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAAc                                                                                                                         >  5:177761/1‑97 (MQ=255)
                                                                         aaGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAAc                                                                                                                         >  1:266571/1‑97 (MQ=255)
                                                                         aaGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAAc                                                                                                                         <  6:177761/97‑1 (MQ=255)
                                                                           gcgcGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGCTTAACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCt                                                                   >  1:288323/1‑149 (MQ=255)
                                                                              cgAACTTCCGTTCGCTCCTTTATAAGTGGCCTGCAGGAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGAATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtc                                                                <  7:126456/149‑1 (MQ=255)
                                                                               gatcTGCGGTTCGCTCATTGATATGGGGCCTGCAGGAGCGGGTTCACCCGCGAATAGGCGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtca                                                               <  8:103873/146‑1 (MQ=37)
                                                                                     cggTTCGCTCCTTGATAAGGGGCCTGCTGGTGCGGGTTCACCCGCTAATACGAGGGTCAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCatcatc                                                          <  6:143452/146‑1 (MQ=38)
                                                                                     ccGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCatcatc                                                          <  2:288323/148‑1 (MQ=255)
                                                                                     ccGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAAAACCAGGGTGAACCCACCGCCGCATTAGCGGGTGAACCCGCGCCGGCAGGGGTCGCCGGCCGCCGTTGCTTACTTGTCGATGCCCGTGCCGTtcatagctc                                                         >  7:103873/1‑144 (MQ=255)
                                                                                     ccGTGCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTTAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTcatcat                                                           <  1:113293/147‑1 (MQ=255)
                                                                                             tCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGa                                                                           <  3:333799/123‑1 (MQ=255)
                                                                                             tCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCGGCAGGGTTCGCCGGCAGCCGTTGATTACTTGACGATGACCTTGCCGTTCATCATCGAGATGTgg                                                 >  7:288014/1‑149 (MQ=255)
                                                                                             tCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGAGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGa                                                                                 >  6:381053/1‑117 (MQ=255)
                                                                                             tCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATACGAGGGTGAACCCCCCCACGCATTAGCGGGTGAACCCGCTCCAGCAGGGTTAGCCGTCAGCCGTTGATTTATTGACGATGa                                                                           >  4:333799/1‑123 (MQ=255)
                                                                                               cTTGATACGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGc                                                                                                                     <  7:305231/79‑1 (MQ=255)
                                                                                                  taTAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGccc                                              <  3:249874/146‑1 (MQ=255)
                                                                                                  gATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCg                                             <  2:273930/148‑1 (MQ=255)
                                                                                                  gATAAGGGGCGTGCAGGAGCGGGCTCACCCGCTACTACGAGGGTCAACCCACCGACGCTTTCCCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGccc                                              <  2:220605/147‑1 (MQ=255)
                                                                                                   aTAAGGGGCCTGCAGGAGGGGTTTCACCCGCGAATACGTGGGTGAACCCACCGACCCATTCGGGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCgg                                            <  8:288014/148‑1 (MQ=39)
                                                                                                         ggCCTGAAGGAGCGGGTCCACCCGCAAATACCAGGTTTAACCCACCCACCCATTCGGGGGTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGt                                      <  1:159759/148‑1 (MQ=255)
                                                                                                          gCCTGGAGGTGGGGGTTCACGCGCGAATACGTGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTg                                     <  5:141257/148‑1 (MQ=38)
                                                                                                                  gAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag                            >  3:359707/1‑149 (MQ=255)
                                                                                                                  gAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTAATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag                            >  3:353791/1‑149 (MQ=255)
                                                                                                                          tCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag                            >  3:151483/1‑141 (MQ=255)
                                                                                                                          tCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag                            <  4:151483/141‑1 (MQ=255)
                                                                                                                            aCCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac                  >  7:219028/1‑149 (MQ=255)
                                                                                                                            aCCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTca                   >  2:150145/1‑148 (MQ=255)
                                                                                                                               cgcgATTACGAGGGTCAACCCACCGACGCATTCGCGGGTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac               <  4:353791/149‑1 (MQ=255)
                                                                                                                               cgcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac               <  4:359707/149‑1 (MQ=255)
                                                                                                                                        gAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTc        >  1:225564/1‑147 (MQ=255)
                                                                                                                                          gggTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGc    <  1:55978/149‑1 (MQ=255)
                                                                                                                                            ggcaaCCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCtt  <  4:14176/146‑1 (MQ=255)
                                                                                                                                            gTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCtt  <  5:180565/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTT  >  NC_002947/5536924‑5537212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑