Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,539,020 G→C 8.5% L656V (CTG→GTG)  PP_4872 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,539,0200GC 8.5% 185.6 / 6.5 59L656V (CTG→GTG) PP_4872hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base C (2/3);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CTCTGATAAGTATCGGTCTGCGGCTGGTGCAGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTACCTGGTCGGCGCGGAAG  >  NC_002947/5538872‑5539165
                                                                                                                                                    |                                                                                                                                                 
ctctGATAAGTATCGGTCTGCGGCTGGTGCAGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAg                                                                                                                                                   <  2:101626/149‑1 (MQ=255)
 tctGATAAGTATCGGTCTGCGGCTGGTGCAGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGa                                                                                                                                                  >  7:31044/1‑149 (MQ=255)
      tAAGTATCGGTCTGCGGCTGGTGCAGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCg                                                                                                                                             <  1:361653/149‑1 (MQ=255)
      tAAGTATCGGTCTGCGGCTGGTGCAGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCg                                                                                                                                             >  2:237255/1‑149 (MQ=255)
         gTATCGGTCTGCGGCTGGTGCAGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTg                                                                                                                                          >  4:115622/1‑149 (MQ=255)
         gTATCGGTCTGCGGCTGGTGCAGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCGTCTGCACAAGGCGttc                                                                                                                                          <  7:339348/149‑2 (MQ=255)
         gTATCGGGCTGCGGCTGGTGCAGCGGGATCAGTTGGTGCTTGCCCAGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTg                                                                                                                                          <  2:356874/149‑1 (MQ=255)
                          gTGCAGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGAc                                                                                                                           <  5:39426/147‑1 (MQ=255)
                             cAGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGAtct                                                                                                                      >  7:28813/1‑149 (MQ=255)
                              aGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCGTCTGCACAAGGCGttctt                                                                                                                                        <  5:239928/130‑4 (MQ=255)
                              aGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCGTCTGCACAAGGCGttctt                                                                                                                                        >  6:239928/1‑127 (MQ=255)
                                   gATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCAccc                                                                                                                <  8:31044/149‑1 (MQ=255)
                                     tCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCt                                                                                                              >  1:17144/1‑149 (MQ=255)
                                        gTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCg                                                                                                           >  6:385156/1‑149 (MQ=255)
                                        gTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCg                                                                                                           >  5:419558/1‑149 (MQ=255)
                                            gTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGAtc                                                                                                                       <  2:157215/133‑1 (MQ=255)
                                            gTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGctct                                                                                                       >  6:210311/1‑149 (MQ=255)
                                            gTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGctc                                                                                                        <  5:348259/148‑1 (MQ=255)
                                            gTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGctc                                                                                                        >  6:348259/1‑148 (MQ=255)
                                            gTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGctc                                                                                                        <  6:419558/148‑1 (MQ=255)
                                             tGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCtt                                                                                                      <  2:155663/149‑1 (MQ=255)
                                             tGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCtt                                                                                                      <  5:210311/149‑1 (MQ=255)
                                                  gcccggcccgATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAg                                                                                                  <  3:300033/148‑1 (MQ=255)
                                                    ccggcccgATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGt                                                                                                >  6:274620/1‑148 (MQ=255)
                                                                 gTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTa                                                                                                   >  3:252704/1‑132 (MQ=255)
                                                                 gTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTa                                                                                                   <  4:252704/132‑1 (MQ=255)
                                                                      ctcggcCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTc                                                                             <  7:174628/149‑1 (MQ=255)
                                                                                aCGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGt                                                                   >  3:270947/1‑149 (MQ=255)
                                                                                 cGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGt                                                                   <  3:115622/148‑1 (MQ=255)
                                                                                  gCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAg                                                                    <  4:242588/146‑1 (MQ=255)
                                                                                  gCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAg                                                                    >  3:242588/1‑146 (MQ=255)
                                                                                         cgcTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACAtgg                                                          >  4:162737/1‑149 (MQ=255)
                                                                                               aCGCAGCATCGGCCAGCCCTTCTGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACAtggccgtgg                                                    >  8:249299/1‑149 (MQ=255)
                                                                                                cGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACAtggccgtggc                                                   >  2:246727/1‑149 (MQ=255)
                                                                                                  cagcaTCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACAtggccgtggccg                                                 >  7:90539/1‑149 (MQ=255)
                                                                                                    gcaTCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAg                                               <  8:90539/149‑1 (MQ=255)
                                                                                                     caTCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAgg                                              <  3:162737/149‑1 (MQ=255)
                                                                                                     caTCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAgg                                              <  1:237255/149‑1 (MQ=255)
                                                                                                     caTCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAgg                                              <  5:385156/149‑1 (MQ=255)
                                                                                                          gCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTg                                                                                                     >  1:170101/1‑89 (MQ=255)
                                                                                                          gCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTg                                                                                                     <  2:170101/89‑1 (MQ=255)
                                                                                                                  ttCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACgg                                 >  5:131046/1‑149 (MQ=255)
                                                                                                                      ggTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACggg                                >  2:253573/1‑146 (MQ=255)
                                                                                                                      ggTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACggg                                >  8:131831/1‑146 (MQ=255)
                                                                                                                       gTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTa                              <  3:392430/147‑1 (MQ=255)
                                                                                                                       gTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTa                              >  4:392430/1‑147 (MQ=255)
                                                                                                                          gTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGctc                                                                                                        >  1:57474/1‑70 (MQ=255)
                                                                                                                          gTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGctc                                                                                                        <  2:57474/70‑1 (MQ=255)
                                                                                                                                gcgcAAGAACGCCTTGTTCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCGACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGt                    <  3:150115/148‑1 (MQ=255)
                                                                                                                                 cgcAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTAc                  <  5:274620/149‑1 (MQ=255)
                                                                                                                                    agcaaCGCCGTCTGCACAAGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACAtggccgtggcct                                                 <  7:154164/112‑2 (MQ=255)
                                                                                                                                    agcaaCGCCGTCTGCACAAGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACAtggccgtggccg                                                 >  8:154164/4‑115 (MQ=255)
                                                                                                                                           ccTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTACCTGGTCGgcg        <  4:270947/149‑1 (MQ=255)
                                                                                                                                            cTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTAc                  >  2:106865/1‑138 (MQ=255)
                                                                                                                                            cTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTACCTGGTCGgcgc       >  4:265906/1‑149 (MQ=255)
                                                                                                                                            cTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTACCTGGTCGgcgc       <  2:235786/149‑1 (MQ=255)
                                                                                                                                            cTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTACCTGGTCGgcgc       <  1:246727/149‑1 (MQ=255)
                                                                                                                                            cTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCCGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTAc                  <  1:106865/138‑1 (MQ=255)
                                                                                                                                             ttGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTACCTGGTCGGcgcg      >  7:227759/1‑149 (MQ=255)
                                                                                                                                                 gCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTACCTGGTCGGCGCGGAAg  >  8:277420/1‑149 (MQ=255)
                                                                                                                                                    gACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTg                                        >  8:109905/1‑108 (MQ=255)
                                                                                                                                                    gACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTg                                        <  7:109905/108‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTCTGATAAGTATCGGTCTGCGGCTGGTGCAGCGGGATCAGTTGGTGCTTGCCCGGCCCGATCAGGTCGGCTCGGCCCATACGCTGCAGCGCTTCACGCAGCATCGGCCAGCCCTTCGGGTCGTGGTAGCGCAAGAACGCCTTGTGCAGACGGCGTTGCTCGTCGCTCTTGACGATCTCCACCCCTTCGCTCTTGTAGGTCACCTTGCGCAGCGGGTTCTTGCCTGAGTGGTACATGGCCGTGGCCGAGGCCATTGGCGACGGGTAGAACGCCTGTACCTGGTCGGCGCGGAAG  >  NC_002947/5538872‑5539165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑