Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,555,423 G→A 6.8% Y34Y (TAC→TAT)  PP_4887 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,555,4230GA 6.8% 217.2 / 2.4 72Y34Y (TAC→TAT) PP_4887hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/37);  new base A (2/3);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GTCCAGGCGCTCACCGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTG  >  NC_002947/5555276‑5555571
                                                                                                                                                   |                                                                                                                                                    
gTCCAGGCGCTCACCGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAg                                                                                                                                                      >  4:61678/1‑148 (MQ=255)
gTCCAGGCGCTCACCGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAg                                                                                                                                                      >  7:276149/1‑148 (MQ=255)
    aGGCGCTCACCGCCGGCTGGCTCATGCAGTGGATCGCCGCGAGTGCAGCCGTGTATATCGGAGACATTGAAACGGCGTGAGATACAGTCGCGCATGCGGACGTCCCGGGCCCGACCGCCGGGTCCGTCCTAGGTCATGGCGCAGGAGGc                                                                                                                                                 >  4:39031/1‑149 (MQ=255)
     ggCGCTCACCGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCt                                                                                                                                                >  6:198980/1‑149 (MQ=255)
      gcgcgcACCGACTGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGATATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGTGACGTCAGAGGTCATGGCGCAGTAGGCtt                                                                                                                                               <  8:104263/149‑1 (MQ=255)
        gcTCACCGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGc                                                                                                                                             <  4:333004/149‑1 (MQ=255)
            aCCGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAggg                                                                                                                                         >  3:264384/1‑149 (MQ=255)
            aCCGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAgg                                                                                                                                          <  7:48460/148‑1 (MQ=255)
             ccGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGc                                                                                                                                        <  3:323352/149‑1 (MQ=255)
              cGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCg                                                                                                                                       >  5:141631/1‑149 (MQ=255)
              cGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAATAGGCTTGCAGGGCg                                                                                                                                       <  1:99125/149‑1 (MQ=255)
              cGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCAGTTGATATCGGCGACAATGAAACGGCGTGAGATAAAGTCGCGAATGCTGAAGGCCCGTGCCAGCCAGCAGGGGACGTCCGAGGTCATCGCGAAGTAGGCTTGCAGGGCg                                                                                                                                       >  6:111828/1‑149 (MQ=255)
                  ggCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCa                                                                                                                                   >  6:56760/1‑149 (MQ=255)
                    cTGGCATATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATc                                                                                                                                 <  7:1480/149‑1 (MQ=255)
                    cTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATc                                                                                                                                 <  1:184621/149‑1 (MQ=255)
                       gctcaTGCATTGGATCGCCGCGAGTGCAGCCGTGGATATCGGCGACAGTGAAACGGCGTGAGATAAAGGCGCGAAGGCGGAAGGCCAGGGCCAGCCATCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCggg                                                                                                                              <  3:39031/146‑1 (MQ=255)
                          caTGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTc                                                                                                                            <  1:155392/148‑1 (MQ=255)
                            tGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGt                                                                                                                         <  6:366558/149‑1 (MQ=255)
                               aTTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGTGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAg                                                                                                                      >  6:300518/1‑149 (MQ=255)
                               aTTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAg                                                                                                                      >  4:160967/1‑149 (MQ=255)
                                     tCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGc                                                                                                                                             >  6:217002/1‑120 (MQ=255)
                                     tCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGc                                                                                                                                             <  5:217002/120‑1 (MQ=255)
                                        ccgcGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCt                                                                                                             >  4:137113/1‑149 (MQ=255)
                                          gcgAGTGAAGCCGTGGATATCGGCGACATTGAAACGGTGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGt                                                                                                           <  5:300518/149‑1 (MQ=255)
                                            gAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAATAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCa                                                                                                         >  1:86132/1‑149 (MQ=255)
                                            gAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAATAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCa                                                                                                         >  1:80357/1‑149 (MQ=255)
                                                       tGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGaa                                                                                              <  5:56760/149‑1 (MQ=255)
                                                        ggATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGaaa                                                                                             <  8:276149/149‑1 (MQ=255)
                                                             tCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGGCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAg                                                                                        <  5:241478/149‑1 (MQ=255)
                                                             tCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAg                                                                                        <  2:217132/149‑1 (MQ=255)
                                                              cGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAgg                                                                                       >  6:395757/1‑149 (MQ=255)
                                                                     aTTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGcgt                                                                                >  5:363753/1‑147 (MQ=255)
                                                                     aTTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTAATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTAACTGTGCAGGAAATTCAGGTCGGCCt                                                                                >  1:413288/1‑149 (MQ=255)
                                                                     aTTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCAAGCAGGGGACGTCCGAGGTCATGGCGCAGTAGGGTTGCAGGGCGGTAATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCt                                                                                <  5:111828/149‑1 (MQ=255)
                                                                     aTTGAAACGGCGTGAGATAAAGTCGCGAATGAGGAAGGCCCGGGCCAGCCAGCCTGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCCGTCATCGGCTCAGTGAGCCGTACGCGGTCACTGTGCAGGAAATTCAGGTCGGCCt                                                                                >  6:396595/1‑149 (MQ=255)
                                                                        gAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCTCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTgg                                                                              <  3:95833/148‑1 (MQ=255)
                                                                                gTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGc                                                                                                   <  4:107378/119‑1 (MQ=255)
                                                                                gTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGc                                                                                                   >  3:107378/1‑119 (MQ=255)
                                                                                 tGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTa                                                                    <  4:264384/149‑1 (MQ=255)
                                                                                    gaTAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAATAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTc                                                                 <  2:86132/149‑1 (MQ=255)
                                                                                    gaTAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAATAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTc                                                                 <  2:80357/149‑1 (MQ=255)
                                                                                     aTAAAGTCGCGAATGCGGAAGGCCCGGGCCACACAGCCGGGGACGTCAGAGGTCATGGAGCAGTAGGCTTGCATCGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTAGGCCTGGGCAGCGATTAGTCg                                                                >  5:414859/1‑149 (MQ=255)
                                                                                       aaaGTCGCGAATGCGGAAGGCCAGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGTCGGTCATCGGGTCAGTGAGCCTTAAGCTGTCACTGTGCAGGAAATTCAGGACTGCCTGGGAAGCGATTAGTCggg                                                              >  8:254339/1‑149 (MQ=255)
                                                                                        aaGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGc                                                             <  6:363753/149‑1 (MQ=255)
                                                                                         aGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTTGTCGGGCg                                                            <  3:61678/149‑1 (MQ=255)
                                                                                         aGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGGCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAggcgggcg                                                            <  5:198980/149‑1 (MQ=255)
                                                                                             gcgAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGAc                                                         <  5:395757/148‑1 (MQ=255)
                                                                                                 aTGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAg                                                    <  6:141631/149‑1 (MQ=255)
                                                                                                    cGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGAt                                                                      >  1:265619/1‑128 (MQ=255)
                                                                                                    cGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGAt                                                                      <  2:265619/128‑1 (MQ=255)
                                                                                                    cGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCaa                                                 >  3:34964/1‑149 (MQ=255)
                                                                                                     ggAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAAc                                                >  3:270720/1‑149 (MQ=255)
                                                                                                     ggAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGAAGCGATTAGTCGGGCGGACATGAGAAAc                                                >  6:349672/1‑149 (MQ=255)
                                                                                                            ccGGGCCAGCCAGCCGGGGACGTCCGAGGTCTTGGCGCAGTAGGCTTTCAGGGCGGTGATCGGCTCAGTGAGCCGTACGCTGTCACTGTGCAGTAATTTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCtttt                                         <  5:396595/149‑1 (MQ=255)
                                                                                                                 ccaggcagCCGGGTTCGTCCGAGGTCATGGCGCAGTTGTGTTGCAGGGCGGTCATCGGGTGAGTTAGCCGTACGCTGTGACTGTGGAGTAAATTGAGGGCGGCCTGGGCAGCGATTAGTCGGGCGGACATGTGCAACTGCTTTTGTCAt                                    <  6:414859/149‑1 (MQ=255)
                                                                                                                  cagccagcCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGc                                                             <  6:13766/123‑1 (MQ=255)
                                                                                                                  cagccagcCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGc                                                             >  5:13766/1‑123 (MQ=255)
                                                                                                                   agccagccGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCAtgt                                  >  5:70422/1‑149 (MQ=255)
                                                                                                                           ggggACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAg                                                    <  2:387645/123‑1 (MQ=255)
                                                                                                                           ggggACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAg                                                    >  1:387645/1‑123 (MQ=255)
                                                                                                                              gACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAg                       <  4:34964/149‑1 (MQ=255)
                                                                                                                                   ccGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACa                        <  5:6386/143‑1 (MQ=255)
                                                                                                                                   ccGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACa                        >  6:6386/1‑143 (MQ=255)
                                                                                                                                   ccGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAg                  >  5:293852/1‑149 (MQ=255)
                                                                                                                                    cGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAg                  >  1:339298/1‑148 (MQ=255)
                                                                                                                                    cGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGGCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGc                 <  6:232264/149‑1 (MQ=255)
                                                                                                                                      aGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAgg                                                                                                >  2:378720/1‑68 (MQ=255)
                                                                                                                                      aGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAgg                                                                                                <  1:378720/68‑1 (MQ=255)
                                                                                                                                        gTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAaca             <  3:160967/149‑1 (MQ=255)
                                                                                                                                        gTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAaca             <  6:70422/149‑1 (MQ=255)
                                                                                                                                         tgaTGTCGCAGTAGGCTTGCAGGGCGGTCATCGGTTCAGTGATCCGTACGCTGTCACTGTGCAGGAAATTCATGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAacac            <  2:413288/147‑1 (MQ=255)
                                                                                                                                         tCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAacac            >  1:173798/1‑149 (MQ=255)
                                                                                                                                              gcgcAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCt        >  4:396342/1‑148 (MQ=255)
                                                                                                                                                 cAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAgg    <  3:137113/149‑1 (MQ=255)
                                                                                                                                                   gTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGgtg  <  6:293852/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTCCAGGCGCTCACCGACGGCTGGCACATGCATTGGATCGCCGCGAGTGAAGCCGTGGATATCGGCGACATTGAAACGGCGTGAGATAAAGTCGCGAATGCGGAAGGCCCGGGCCAGCCAGCCGGGGACGTCCGAGGTCATGGCGCAGTAGGCTTGCAGGGCGGTCATCGGGTCAGTGAGCCGTACGCTGTCACTGTGCAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTG  >  NC_002947/5555276‑5555571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑