Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,570,178 G→C 6.8% A24G (GCA→GGA) ‡ nnrD ← ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,570,1780GC 6.8% 286.5 / 9.5 88A24G (GCA→GGA) ‡nnrDADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (41/41);  new base C (3/3);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

CTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCCTCTA  >  NC_002947/5570033‑5570326
                                                                                                                                                 |                                                                                                                                                    
cTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCtgccgtgccg                                                                                                                                                   >  3:334894/1‑149 (MQ=255)
 tCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCgg                                                                                                                                                  >  4:26425/1‑149 (MQ=255)
    ggCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGGCACGGCAg                                                                                                                                               >  5:256553/1‑149 (MQ=255)
     gCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAgg                                                                                                                                              <  3:26425/149‑1 (MQ=255)
      ccGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGGCACGGCAGGc                                                                                                                                             <  8:304594/149‑1 (MQ=255)
      ccGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGGCACGGCAGGc                                                                                                                                             <  3:401676/149‑1 (MQ=255)
        gggTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCgg                                                                                                                                           <  3:80337/149‑1 (MQ=255)
        gggTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGGCACGGCAGGCgc                                                                                                                                           <  6:256553/149‑2 (MQ=255)
                 cGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTc                                                                                                                                  <  4:334894/149‑1 (MQ=255)
                  gCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCa                                                                                                                                 >  3:293334/1‑149 (MQ=255)
                   cTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCAc                                                                                                                                >  5:99875/1‑149 (MQ=255)
                    tGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACg                                                                                                                               >  8:215799/1‑149 (MQ=255)
                        cAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACgctgc                                                                                                                           >  7:303598/1‑149 (MQ=255)
                         aGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACgctgct                                                                                                                          <  8:330384/149‑1 (MQ=255)
                         aGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACgctgct                                                                                                                          >  7:57361/1‑149 (MQ=255)
                          gCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACgctgct                                                                                                                          >  8:270334/1‑148 (MQ=255)
                           cccGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGa                                                                                                                        <  7:215799/149‑1 (MQ=255)
                           cccGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAATACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGa                                                                                                                        <  6:254212/149‑1 (MQ=255)
                                    gcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATtgtg                                                                                                               >  7:197105/1‑149 (MQ=255)
                                                ggcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgg                                                                                                   >  2:340223/1‑149 (MQ=255)
                                                 gcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                                  <  2:181122/149‑1 (MQ=255)
                                                        cACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGCCCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg                                                                                           >  2:258911/1‑149 (MQ=255)
                                                         aCTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCTTTAAGGGGGTGGTTgg                                                                                          >  8:340834/1‑149 (MQ=255)
                                                             aGCAACACAGCGCCTCCGGTA‑CCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCt                                                                                       <  8:42299/147‑1 (MQ=255)
                                                              gCAACACAGCGCCTCCTGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAACGTCGCCTTTGTGGGCGTGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGc                                                                                     <  5:310648/149‑1 (MQ=255)
                                                                aaCACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCgg                                                                                   >  2:74811/1‑149 (MQ=255)
                                                                 acacAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTgg                                                                                                              <  5:86933/121‑1 (MQ=255)
                                                                 acacAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTgg                                                                                                              >  6:86933/1‑121 (MQ=255)
                                                                 acacAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCgg                                                                                   >  5:192704/1‑148 (MQ=255)
                                                                  cacaGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCa                                                                                 <  7:270334/149‑1 (MQ=255)
                                                                   acaGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCAt                                                                                <  8:303598/149‑1 (MQ=255)
                                                                      gcgcCTCCGGTACCCAGGGCGCCCCCGACCACCAGTACATGGACAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                             <  8:197105/149‑1 (MQ=255)
                                                                        gcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGt                                                                           >  5:286419/1‑149 (MQ=255)
                                                                        gcCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCCCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTCACCAATTCTggg                                                                                                             >  4:277422/1‑115 (MQ=255)
                                                                            ccGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCa                                                                         >  6:84836/1‑147 (MQ=255)
                                                                             cGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCaaa                                                                       <  1:74811/148‑1 (MQ=255)
                                                                                 aCCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCa                                                                         <  7:28512/142‑1 (MQ=255)
                                                                                 aCCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCa                                                                         >  8:28512/1‑142 (MQ=255)
                                                                                   ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTc                                                                                                                                  <  2:282621/83‑1 (MQ=255)
                                                                                   ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTc                                                                                                                                  >  1:282621/1‑83 (MQ=255)
                                                                                   ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCGATTCTGGGGCATTTCTGGGGTGGTTGGTCTGCGGGATGGGGTCAAAGGCTcc                                                                 >  5:423798/1‑148 (MQ=255)
                                                                                   ccAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTcc                                                                 >  1:199963/1‑148 (MQ=255)
                                                                                     aGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                             <  8:363053/134‑1 (MQ=255)
                                                                                     aGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                             >  7:363053/1‑134 (MQ=255)
                                                                                           cccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                        <  6:192704/149‑1 (MQ=255)
                                                                                           cccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTg                                                         >  7:70475/1‑148 (MQ=255)
                                                                                            ccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATg                                                                               >  4:141931/1‑125 (MQ=255)
                                                                                            ccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                        >  2:260261/1‑148 (MQ=255)
                                                                                            ccccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                        >  4:148090/1‑148 (MQ=255)
                                                                                             cccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGc                                                       >  8:313357/1‑148 (MQ=255)
                                                                                             cccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCa                                                      <  6:99875/149‑1 (MQ=255)
                                                                                             cccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTACCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGa                                                                                                                        <  1:173206/83‑1 (MQ=255)
                                                                                             cccGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTACCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGa                                                                                                                        >  2:173206/1‑83 (MQ=255)
                                                                                                gACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                        >  3:293879/1‑144 (MQ=255)
                                                                                                gACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                        <  4:293879/144‑1 (MQ=255)
                                                                                                gACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGaa                                                   >  8:295102/1‑149 (MQ=255)
                                                                                                    accaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCaaa                                                                       <  6:227574/125‑1 (MQ=255)
                                                                                                    accaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCaaa                                                                       >  5:227574/1‑125 (MQ=255)
                                                                                                     ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                             <  8:301608/86‑1 (MQ=255)
                                                                                                     ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATgggg                                                                            <  7:128641/119‑1 (MQ=255)
                                                                                                     ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATgggg                                                                            >  8:128641/1‑119 (MQ=255)
                                                                                                     ccaGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata                                              >  5:112963/1‑149 (MQ=255)
                                                                                                      caGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATAc                                             >  3:130871/1‑149 (MQ=255)
                                                                                                       aGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACg                                            >  2:5493/1‑149 (MQ=255)
                                                                                                            aTGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCt                                       <  7:295102/149‑1 (MQ=255)
                                                                                                               gCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGa                                     <  4:293334/148‑1 (MQ=255)
                                                                                                                ccAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGc                                   <  1:258911/149‑1 (MQ=255)
                                                                                                                 cAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCaaa                                                                       <  5:22009/112‑1 (MQ=255)
                                                                                                                 cAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCaaa                                                                       >  6:22009/1‑112 (MQ=255)
                                                                                                                 cAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                        <  1:145595/127‑1 (MQ=255)
                                                                                                                 cAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                                        >  2:145595/1‑127 (MQ=255)
                                                                                                                 cAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGcc                                  >  7:232731/1‑149 (MQ=255)
                                                                                                                    aGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCaaa                                                                       <  3:79712/109‑1 (MQ=255)
                                                                                                                    aGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCaaa                                                                       >  4:79712/1‑109 (MQ=255)
                                                                                                                     gTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTa                              <  8:57361/149‑1 (MQ=255)
                                                                                                                        gCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAtt                            <  3:148090/148‑1 (MQ=255)
                                                                                                                         ccTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgc                          <  1:5493/149‑1 (MQ=255)
                                                                                                                         ccTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgc                          <  7:313357/149‑1 (MQ=255)
                                                                                                                          cTTTGTGGGGATGCGGCTGCCGTGCCGGCAGGCGGGGAACCGTCACGCGGCTGAGCAATTGTGTGGCATTAGTGGGGTGTTTGGGCTGCGGCTTGGGGTCAAAGGCTGCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgcc                         <  6:423798/149‑1 (MQ=255)
                                                                                                                          cTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgcc                         <  6:286419/149‑1 (MQ=255)
                                                                                                                             tgtgGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgcctt                       <  5:129909/148‑1 (MQ=255)
                                                                                                                              gtgGGCATGGGGCTGCGGTGCCGGCAGGCGGGCAATCGTCACGCTGCTGTTCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCTAAGGCTCCAATGTCTGGCTGTATTATACGCACCTGAGCCCGTAttgccttg                      <  3:278294/148‑1 (MQ=255)
                                                                                                                                gggCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGTCTCCAATGTCTGGCATAATTATACGCACCTGAGCGCGTAttgccttgcc                    >  4:60209/1‑148 (MQ=255)
                                                                                                                                    aTGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata                                              <  3:275530/118‑1 (MQ=255)
                                                                                                                                    aTGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATtata                                              >  4:275530/1‑118 (MQ=255)
                                                                                                                                    aTGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatcc               <  8:232731/149‑1 (MQ=255)
                                                                                                                                    aTGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatcc               <  2:199963/149‑1 (MQ=255)
                                                                                                                                      ccgcCTGCCGGCACGGCAGGCGGGCAAACGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCAAATGTCTGGCAGAATTATACGCACCTGATCCCGTATTGCGTTGAccatcgat             >  6:129170/2‑149 (MQ=255)
                                                                                                                                        gcCTGCCGGCACGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGt           >  1:250288/1‑149 (MQ=255)
                                                                                                                                                 gccgGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCCTCTa  >  8:297410/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAGTCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGTCCGCCTCTA  >  NC_002947/5570033‑5570326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑