Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,584,537 T→A 8.3% I399F (ATC→TTC)  parC ← DNA topoisomerase IV subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,584,5370TA 8.3% 209.4 / 6.4 72I399F (ATC→TTC) parCDNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand):  ref base T (22/44);  new base A (3/3);  total (25/47)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

AGCAACTCATCCTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGG  >  NC_002947/5584389‑5584677
                                                                                                                                                       |                                                                                                                                            
aGCAACTCATCCTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGgatgat                                                                                                                                              <  4:214671/149‑1 (MQ=255)
 gCAACTCATCCTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGAACAGGGCCTGCTTGG‑GATATTCCTCGGTACGGatgatg                                                                                                                                             <  5:370011/149‑1 (MQ=255)
   aaCTCATCCTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGAtgtg                                                                                                                                           <  7:217239/149‑1 (MQ=255)
    aCTCATCCTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTgg                                                                                                                                          <  8:143762/149‑1 (MQ=255)
         tCCTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCAc                                                                                                                                     <  8:34521/149‑1 (MQ=255)
           cTGCTCACCGCGGATTTTCTTTTCTTCCAGTCGCGCCAATTGCCGATGGTGGGTTTTGTGTATTTAGCCTCNGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCAc                                                                                                                                     <  1:131064/149‑1 (MQ=255)
           cTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGGCGATATTCCTCGGTACGGATGATGTGGATCACt                                                                                                                                    <  1:257192/149‑1 (MQ=255)
                cACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑GCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGAAGTGATCCACATCATcc                                                                                                                              <  1:152285/149‑1 (MQ=255)
                 aCCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGACAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATc                                                                                                                               >  1:75901/1‑147 (MQ=255)
                  ccGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATACTCCTCGGTACGGATGAAGTGATCCACATCATccgt                                                                                                                            <  8:2798/149‑3 (MQ=255)
                         tCTTCATCTCTTCCAGGCGCGCAAACTGCCGGAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAg                                                                                                                      <  8:278547/148‑1 (MQ=255)
                          cTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGAtgtg                                                                                                                                           <  8:13350/126‑1 (MQ=255)
                          cTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGAtgtg                                                                                                                                           >  7:13350/1‑126 (MQ=255)
                           ttCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTc                                                                                                                                  >  5:157867/1‑134 (MQ=255)
                           ttCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTc                                                                                                                                  <  6:157867/134‑1 (MQ=255)
                            tCATCTCTTCCAGGCGCGCCAACTCCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTAAGGATGATGTGGAGCACTTCATCCAGGTGGAGGAAt                                                                                                                  >  8:309963/1‑149 (MQ=255)
                             cATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAgg                                                                                                                     <  4:154770/145‑1 (MQ=255)
                             cATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAg                                                                                                                      <  3:58780/144‑1 (MQ=255)
                             cATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATg                                                                                                                 >  3:387480/1‑149 (MQ=255)
                             cATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑GCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAAt                                                                                                                  <  8:261943/148‑1 (MQ=255)
                              atatctTCCAGGCGCGCCAACTGCCGCAGGCGGGGCTCGAGGATTTAG‑‑TCTGACTGTATCTCGGTCAGCCCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATACCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATg                                                                                                                 <  4:76728/145‑1 (MQ=255)
                                ctctTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGAAGTGATCCACATCATccgtac                                                                                                                          <  1:301471/137‑5 (MQ=255)
                                ctctTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGAAGTGATCCACATCATccgtac                                                                                                                          >  2:301471/1‑133 (MQ=255)
                                 tctTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGt                                                                                                             >  7:76008/1‑149 (MQ=255)
                                   ttgcAGGCGCGGCTACTGCCGCAGGCGGGTCTCGAGGCTGTAG‑‑GCGTCTTGTTTGTCTGTCATCTCGATACGTTCGATGAGGGCCTGCTTTG‑GATATTGCTCGGTACGGATGATGTGGTTCACTTGATCCAGGTTGAGGCATGCAg                                                                                                              <  8:316808/143‑1 (MQ=255)
                                   ttCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCa                                                                                                               <  1:2051/145‑1 (MQ=255)
                                   ttCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGt                                                                                                             <  8:76008/147‑1 (MQ=255)
                                   ttCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGt                                                                                                             >  3:170341/1‑147 (MQ=255)
                                    tCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAg                                                                                                          >  4:131382/1‑149 (MQ=255)
                                     ccAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGACAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAg                                                                                                          <  2:75901/148‑1 (MQ=255)
                                          cgcgcCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAAcc                                                                                                    <  3:385751/149‑1 (MQ=255)
                                           gcgcCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATc                                                                                                                               <  8:105471/121‑1 (MQ=255)
                                           gcgcCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATc                                                                                                                               >  7:105471/1‑121 (MQ=255)
                                           gcgcCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCg                                                                                                   <  2:219230/149‑1 (MQ=255)
                                           gcgcCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAAAGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATACAGGTTGAGGAATGCAGTGAGCAAAcct                                                                                                   >  8:354045/1‑148 (MQ=255)
                                             gcCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCAc                                                                                                                                     >  7:53134/1‑113 (MQ=255)
                                             gcCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCAc                                                                                                                                     <  8:53134/113‑1 (MQ=255)
                                             gcCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTc                                                                                                 <  1:33729/149‑1 (MQ=255)
                                                  cTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACa                                                                                            <  2:320616/149‑1 (MQ=255)
                                                      cGCAGGCGGGTCTCGAGGATGTTG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGTTGTGGATCACTTGATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTg                                                                                        <  3:56420/149‑1 (MQ=255)
                                                      cGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTg                                                                                        >  6:393806/1‑149 (MQ=255)
                                                       gCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGc                                                                                       <  2:238117/149‑1 (MQ=255)
                                                                    gAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCa                                                                                                               <  6:173164/112‑1 (MQ=255)
                                                                     aGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGAc                                                                         <  2:229388/149‑1 (MQ=255)
                                                                      ggATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGAcc                                                                        <  4:170341/149‑1 (MQ=255)
                                                                         tGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTa                                                                     <  5:393806/149‑1 (MQ=255)
                                                                                 cGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGa                                                               <  3:131382/149‑1 (MQ=255)
                                                                                  ggCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGa                                                               <  2:193374/148‑1 (MQ=255)
                                                                                          tctcGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTc                                                                                                 <  3:24226/106‑1 (MQ=255)
                                                                                          tctcGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTc                                                                                                 >  4:24226/1‑106 (MQ=255)
                                                                                           ctcGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGAtgctg                                                       >  4:383778/1‑147 (MQ=255)
                                                                                            tcGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCaaa                                                                                                      >  5:349453/1‑99 (MQ=255)
                                                                                            tcGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGAGGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCaaa                                                                                                      <  6:349453/99‑1 (MQ=255)
                                                                                              ggTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCa                                                     <  5:55903/146‑1 (MQ=255)
                                                                                               gTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCAAGACGATGCTGCAggcg                                                 >  3:49452/1‑149 (MQ=255)
                                                                                                     tggAAACGTTCGATCTGGGCCTGCTTGG‑GATATTCCTCGGTACGGATTATGTGGATCACTTCATCCAGGTTGAGGAATGCAGt                                                                                                             <  2:367057/81‑1 (MQ=255)
                                                                                                       gAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACGGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGcgacg                                           <  7:309963/147‑1 (MQ=255)
                                                                                                       gAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGaa                                         >  4:51398/1‑149 (MQ=255)
                                                                                                           cGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGt                                     <  3:383778/149‑1 (MQ=255)
                                                                                                             ttCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGTATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTAt                                   <  5:132496/149‑1 (MQ=255)
                                                                                                                gATCAGGGCCTTCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATTCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTg                                 <  7:354045/148‑1 (MQ=255)
                                                                                                                 aTCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGt                               <  3:51398/149‑1 (MQ=255)
                                                                                                                     gggCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGAtgctg                                                       >  7:291532/1‑121 (MQ=255)
                                                                                                                     gggCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGAtgctg                                                       <  8:291532/121‑1 (MQ=255)
                                                                                                                     gggCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCg                            >  1:57974/1‑148 (MQ=255)
                                                                                                                          tGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTc                      >  4:188548/1‑149 (MQ=255)
                                                                                                                           gCTTTG‑GATATTGCTCTGTACTGATGATGTTTTTCACTTCATCCAGGTTGTGGAATGCATTTTGCAAACCGTCCAACTGGTGCAGACGCTTCTCGACCTTATCCAGACTATGCTGCAGGCGGCGACGAACGGTATTGTTGCGGAACt                       <  2:368539/147‑1 (MQ=255)
                                                                                                                            cTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTc                      <  4:387480/147‑1 (MQ=255)
                                                                                                                               gg‑gATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGTGACGAACGGTATTGGTGCGGAACTCCAGc                  >  2:52707/1‑148 (MQ=255)
                                                                                                                                  gATATTCCTCGGTACGGATGATGTTGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACGGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACt                       <  5:252326/141‑1 (MQ=255)
                                                                                                                                  gATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGTGACGAACGGTATTGGTGCGGAACTCCAGCCAc               <  1:52707/149‑1 (MQ=255)
                                                                                                                                  gATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGAAGCTGAAGGCGGCGACGAACGGTATTCGTGCGGAACt                       >  6:252326/1‑141 (MQ=255)
                                                                                                                                      ttCCTCGGTACGGATGAAGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTATCGCCCTTATCCAGACGATGCTGCAGGCGACGACCAAAGGTATTGCTGAGGAACTCCAGCCACTcca           >  5:827/1‑149 (MQ=255)
                                                                                                                                       tCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAAc                                                                                             <  4:2696/66‑1 (MQ=255)
                                                                                                                                       tCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAAc                                                                                             >  3:2696/1‑66 (MQ=255)
                                                                                                                                              tACGTATTATGAGTATCACTTCATCCAGTTTGAGGAATGCAGTGAGCAAACCGTCGAAAAGTTGCAGACGCTTCTGGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTTGTGCGGAACTCCAGCCACTGCACCAATAgg   <  6:827/149‑1 (MQ=255)
                                                                                                                                               ctggATGAAGTGATCCACATCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAggg  >  2:187681/3‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                            
AGCAACTCATCCTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAG‑‑TCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGG  >  NC_002947/5584389‑5584677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑