Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,584,547 T→A 6.8% E395D (GAA→GAT)  parC ← DNA topoisomerase IV subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,584,5470TA 6.8% 223.3 / 4.8 73E395D (GAA→GAT) parCDNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand):  ref base T (28/40);  new base A (2/3);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

CTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACT  >  NC_002947/5584400‑5584695
                                                                                                                                                    |                                                                                                                                                    
cTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGGCGATATTCCTCGGTACGGATGATGTGGATCACt                                                                                                                                                      <  1:257192/149‑1 (MQ=255)
     cACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGGCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGAAGTGATCCACATCATcc                                                                                                                                                <  1:152285/149‑1 (MQ=255)
      aCCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGACAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATc                                                                                                                                                 >  1:75901/1‑147 (MQ=255)
       ccGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATACTCCTCGGTACGGATGAAGTGATCCACATCATccgt                                                                                                                                              <  8:2798/149‑3 (MQ=255)
              tCTTCATCTCTTCCAGGCGCGCAAACTGCCGGAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAg                                                                                                                                        <  8:278547/148‑1 (MQ=255)
                ttCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTc                                                                                                                                                    >  5:157867/1‑134 (MQ=255)
                ttCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTc                                                                                                                                                    <  6:157867/134‑1 (MQ=255)
                 tCATCTCTTCCAGGCGCGCCAACTCCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTAAGGATGATGTGGAGCACTTCATCCAGGTGGAGGAAt                                                                                                                                    >  8:309963/1‑149 (MQ=255)
                  cATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAgg                                                                                                                                       <  4:154770/145‑1 (MQ=255)
                  cATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAg                                                                                                                                        <  3:58780/144‑1 (MQ=255)
                  cATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATg                                                                                                                                   >  3:387480/1‑149 (MQ=255)
                  cATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGGCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAAt                                                                                                                                    <  8:261943/148‑1 (MQ=255)
                   atatctTCCAGGCGCGCCAACTGCCGCAGGCGGGGCTCGAGGATTTAGTCTGACTGTATCTCGGTCAGCCCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATACCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATg                                                                                                                                   <  4:76728/145‑1 (MQ=255)
                     ctctTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGAAGTGATCCACATCATccgtac                                                                                                                                            >  2:301471/1‑133 (MQ=255)
                     ctctTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGAAGTGATCCACATCATccgtac                                                                                                                                            <  1:301471/137‑5 (MQ=255)
                      tctTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGt                                                                                                                               >  7:76008/1‑149 (MQ=255)
                        ttgcAGGCGCGGCTACTGCCGCAGGCGGGTCTCGAGGCTGTAGGCGTCTTGTTTGTCTGTCATCTCGATACGTTCGATGAGGGCCTGCTTTG‑GATATTGCTCGGTACGGATGATGTGGTTCACTTGATCCAGGTTGAGGCATGCAg                                                                                                                                <  8:316808/143‑1 (MQ=255)
                        ttCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCa                                                                                                                                 <  1:2051/145‑1 (MQ=255)
                        ttCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGt                                                                                                                               >  3:170341/1‑147 (MQ=255)
                        ttCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGt                                                                                                                               <  8:76008/147‑1 (MQ=255)
                         tCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAg                                                                                                                            >  4:131382/1‑149 (MQ=255)
                          ccAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGACAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAg                                                                                                                            <  2:75901/148‑1 (MQ=255)
                               cgcgcCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAAcc                                                                                                                      <  3:385751/149‑1 (MQ=255)
                                gcgcCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATc                                                                                                                                                 >  7:105471/1‑121 (MQ=255)
                                gcgcCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATc                                                                                                                                                 <  8:105471/121‑1 (MQ=255)
                                gcgcCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCg                                                                                                                     <  2:219230/149‑1 (MQ=255)
                                gcgcCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAAAGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATACAGGTTGAGGAATGCAGTGAGCAAAcct                                                                                                                     >  8:354045/1‑148 (MQ=255)
                                  gcCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTc                                                                                                                   <  1:33729/149‑1 (MQ=255)
                                       cTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACa                                                                                                              <  2:320616/149‑1 (MQ=255)
                                           cGCAGGCGGGTCTCGAGGATGTTGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGTTGTGGATCACTTGATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTg                                                                                                          <  3:56420/149‑1 (MQ=255)
                                           cGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTg                                                                                                          >  6:393806/1‑149 (MQ=255)
                                            gCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGc                                                                                                         <  2:238117/149‑1 (MQ=255)
                                                         gAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCa                                                                                                                                 <  6:173164/112‑1 (MQ=255)
                                                          aGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGAc                                                                                           <  2:229388/149‑1 (MQ=255)
                                                           ggATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGAcc                                                                                          <  4:170341/149‑1 (MQ=255)
                                                              tGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTa                                                                                       <  5:393806/149‑1 (MQ=255)
                                                                    cGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGa                                                                                 <  3:131382/149‑1 (MQ=255)
                                                                     ggCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGa                                                                                 <  2:193374/148‑1 (MQ=255)
                                                                             tctcGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTc                                                                                                                   <  3:24226/106‑1 (MQ=255)
                                                                             tctcGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTc                                                                                                                   >  4:24226/1‑106 (MQ=255)
                                                                              ctcGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGAtgctg                                                                         >  4:383778/1‑147 (MQ=255)
                                                                               tcGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCaaa                                                                                                                        >  5:349453/1‑99 (MQ=255)
                                                                               tcGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGAGGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCaaa                                                                                                                        <  6:349453/99‑1 (MQ=255)
                                                                                 ggTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCa                                                                       <  5:55903/146‑1 (MQ=255)
                                                                                  gTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCAAGACGATGCTGCAggcg                                                                   >  3:49452/1‑149 (MQ=255)
                                                                                        tggAAACGTTCGATCTGGGCCTGCTTGG‑GATATTCCTCGGTACGGATTATGTGGATCACTTCATCCAGGTTGAGGAATGCAGt                                                                                                                               <  2:367057/81‑1 (MQ=255)
                                                                                          gAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACGGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGcgacg                                                             <  7:309963/147‑1 (MQ=255)
                                                                                          gAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGaa                                                           >  4:51398/1‑149 (MQ=255)
                                                                                              cGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGt                                                       <  3:383778/149‑1 (MQ=255)
                                                                                                ttCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGTATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTAt                                                     <  5:132496/149‑1 (MQ=255)
                                                                                                   gATCAGGGCCTTCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATTCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTg                                                   <  7:354045/148‑1 (MQ=255)
                                                                                                    aTCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGt                                                 <  3:51398/149‑1 (MQ=255)
                                                                                                        gggCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGAtgctg                                                                         >  7:291532/1‑121 (MQ=255)
                                                                                                        gggCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGAtgctg                                                                         <  8:291532/121‑1 (MQ=255)
                                                                                                        gggCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCg                                              >  1:57974/1‑148 (MQ=255)
                                                                                                             tGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTc                                        >  4:188548/1‑149 (MQ=255)
                                                                                                              gCTTTG‑GATATTGCTCTGTACTGATGATGTTTTTCACTTCATCCAGGTTGTGGAATGCATTTTGCAAACCGTCCAACTGGTGCAGACGCTTCTCGACCTTATCCAGACTATGCTGCAGGCGGCGACGAACGGTATTGTTGCGGAACt                                         <  2:368539/147‑1 (MQ=255)
                                                                                                               cTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTc                                        <  4:387480/147‑1 (MQ=255)
                                                                                                                  gg‑gATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGTGACGAACGGTATTGGTGCGGAACTCCAGc                                    >  2:52707/1‑148 (MQ=255)
                                                                                                                     gATATTCCTCGGTACGGATGATGTTGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACGGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACt                                         <  5:252326/141‑1 (MQ=255)
                                                                                                                     gATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGTGACGAACGGTATTGGTGCGGAACTCCAGCCAc                                 <  1:52707/149‑1 (MQ=255)
                                                                                                                     gATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGAAGCTGAAGGCGGCGACGAACGGTATTCGTGCGGAACt                                         >  6:252326/1‑141 (MQ=255)
                                                                                                                         ttCCTCGGTACGGATGAAGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTATCGCCCTTATCCAGACGATGCTGCAGGCGACGACCAAAGGTATTGCTGAGGAACTCCAGCCACTcca                             >  5:827/1‑149 (MQ=255)
                                                                                                                          tCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAAc                                                                                                               >  3:2696/1‑66 (MQ=255)
                                                                                                                          tCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAAc                                                                                                               <  4:2696/66‑1 (MQ=255)
                                                                                                                                 tACGTATTATGAGTATCACTTCATCCAGTTTGAGGAATGCAGTGAGCAAACCGTCGAAAAGTTGCAGACGCTTCTGGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTTGTGCGGAACTCCAGCCACTGCACCAATAgg                     <  6:827/149‑1 (MQ=255)
                                                                                                                                  ctggATGAAGTGATCCACATCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAggg                    >  2:187681/3‑149 (MQ=255)
                                                                                                                                           gtgGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGt           >  3:156357/1‑149 (MQ=255)
                                                                                                                                             ggATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGAGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGttt         >  5:255008/1‑149 (MQ=255)
                                                                                                                                             ggATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGAGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGttt         >  1:308911/1‑149 (MQ=255)
                                                                                                                                              gATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCAc                                 <  3:196941/124‑1 (MQ=255)
                                                                                                                                              gATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCAc                                 >  4:196941/1‑124 (MQ=255)
                                                                                                                                                ccacaTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGttttt       <  1:187681/147‑1 (MQ=255)
                                                                                                                                                   cTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAAc   >  6:380131/1‑149 (MQ=255)
                                                                                                                                                   cTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAAc   >  3:2485/1‑149 (MQ=255)
                                                                                                                                                    ttCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACt  <  4:156357/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGCTCACCGCGGATCTTCATCTCTTCCAGGCGCGCCAACTGCCGCAGGCGGGTCTCGAGGATGTAGTCGGCCTGTATCTCGGTCAGCTCGAAACGTTCGATCAGGGCCTGCTTGG‑GATATTCCTCGGTACGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACT  >  NC_002947/5584400‑5584695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑