| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,608,307 | T→A | 12.5% | intergenic (‑169/+19) | cyaB ← / ← waaA | cyclolysin secretion/processing ATP‑binding protein CyaB/3‑deoxy‑D‑manno‑octulosonic acid transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,608,307 | 0 | T | A | 12.5% | 141.3 / 11.9 | 56 | intergenic (‑169/+19) | cyaB/waaA | cyclolysin secretion/processing ATP‑binding protein CyaB/3‑deoxy‑D‑manno‑octulosonic acid transferase |
| Reads supporting (aligned to +/- strand): ref base T (24/24); new base A (4/3); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01 | |||||||||||
CTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTCCACCAGGCGGCGCACCG > NC_002947/5608159‑5608453 | cTTCCGACTCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCGAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATATCCAGAATCAGAGATATCCACTC‑T‑AGACCGGGTCttt < 8:48313/149‑1 (MQ=255)cTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGGCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCttt < 6:306047/149‑1 (MQ=255) ttCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCttt > 1:166727/1‑148 (MQ=255) tCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCttt > 2:190997/1‑147 (MQ=255) ccGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTg < 3:177561/147‑1 (MQ=255) cGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAg < 1:190997/148‑1 (MQ=255) cGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGt > 6:311488/1‑149 (MQ=255) cGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGt < 2:166727/149‑1 (MQ=255) cGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGACGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGt > 7:156262/1‑149 (MQ=255) aaGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTCAA‑AGACCCGGTC‑TAGAGTGGatat < 7:84444/149‑1 (MQ=39) aaGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGt > 4:23454/1‑143 (MQ=255) aaGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGt < 3:23454/143‑1 (MQ=255) gtttgACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTTAGGGCGAGAAATGCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCg < 6:278385/146‑1 (MQ=255) tttAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAg > 1:398618/1‑149 (MQ=255) tAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAgg < 7:191783/148‑1 (MQ=255) aaGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTCAA‑AGACCCGGTC‑TAGAGTGGATATGTctga < 4:89807/130‑4 (MQ=37) aaGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTCAA‑AGACCCGGTC‑TAGAGTGGATATGTctga > 3:89807/1‑127 (MQ=37) gTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTc < 8:156262/149‑1 (MQ=255) tAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTc > 2:58672/1‑148 (MQ=255) ggAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGa < 1:58672/148‑1 (MQ=255) ggAAGTTTCCTAACGGAGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAAGCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGTATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGa < 3:80585/148‑1 (MQ=255) aaCGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGc > 1:349284/1‑148 (MQ=255) gaAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGccccc < 2:398618/149‑1 (MQ=255) tCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGAt > 1:72456/1‑149 (MQ=255) tCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGAt < 5:311488/149‑1 (MQ=255) aGTGTAGGAAGATACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTgg < 5:150872/149‑1 (MQ=255) tAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCAACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCACCAGACCCTCGAGCAATCGCTGCAAAGCCACATGATTGGCCCg > 2:197972/1‑149 (MQ=255) gAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGAAGATAGCCCCCAGACCATAGAGCAATCGCTGCAAAGCCCCCGGATTGGCCCGCAt > 1:338106/1‑149 (MQ=255) aaGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATc < 7:287777/149‑1 (MQ=255) ttACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGTGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGa < 8:240297/104‑1 (MQ=255) ttACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGa > 7:240297/1‑104 (MQ=255) ttACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGCTCTTTGAGTGGATATGTCGGTTATCAGCGCATCAACGCCCCCAGa > 7:246689/1‑104 (MQ=255) cTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGCCACTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTg > 5:41458/1‑149 (MQ=255) ttACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGcc < 6:43947/145‑1 (MQ=255) ttACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGcc > 5:43947/1‑145 (MQ=255) aCGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGcc < 1:197972/148‑1 (MQ=255) aCGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGc < 2:349284/147‑1 (MQ=255) gCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGGGGATCAAGGCGCCCAGGCCCTCGAGCAATCGCTGGCAAGCCCCCTGAGAGGCCCGCATCCCGgcccggcccga > 6:224777/1‑148 (MQ=255) gAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCAc > 5:70151/1‑147 (MQ=255) gAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCAc < 6:70151/147‑1 (MQ=255) taaataaCCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATg > 4:66368/1‑149 (MQ=255) ataaCCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGa > 5:414767/1‑87 (MQ=255) ataaCCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGa < 6:414767/87‑1 (MQ=255) aCCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTTGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTgg < 5:224777/149‑1 (MQ=255) aGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGc < 3:336599/83‑1 (MQ=255) aGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGc > 4:336599/1‑83 (MQ=255) cgACATATCCACTCAA‑AGACCCGGTC‑TAGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGc > 4:153161/2‑149 (MQ=38) gACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCg > 4:320993/1‑149 (MQ=255) gACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCg > 2:412985/1‑149 (MQ=255) aCATATCCACTCAA‑AGACCCGGTC‑TAGAGTGGAATTGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCATAGCCCACTGATAGGCCCCCATCACCGCCCTGCCCGCCACACCCAAGCGCTGCGCGTCCAGCgg > 1:262760/1‑149 (MQ=255) tCCACTC‑AAAGACCCGGTC‑TAGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCg < 6:98311/143‑1 (MQ=39) tCCACTC‑AAAGACCCGGTC‑TAGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCg > 5:98311/1‑143 (MQ=39) ccACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGt > 4:401038/1‑147 (MQ=255) ccACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGt < 3:401038/147‑1 (MQ=255) tc‑t‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCgg > 2:339721/1‑139 (MQ=255) tc‑t‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCgg < 1:339721/139‑1 (MQ=255) t‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCCGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTCCACCAg > 7:160060/1‑149 (MQ=255) t‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTgg > 5:348181/1‑126 (MQ=255) t‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTgg < 6:348181/126‑1 (MQ=255) gACCGGGTCTTCGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTCCACCAggc > 4:200069/1‑149 (MQ=255) tttGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACACTACAGCAATCGCTGCAAAGACCCCAGATTGGCCCTCATCACAGACCTGCCCCCCTCACCCATGCGATGGGCGTACTGCGGCAGTTCCACAAGCCGGCGCACcg > 3:373825/1‑149 (MQ=255) | CTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑T‑AGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTCCACCAGGCGGCGCACCG > NC_002947/5608159‑5608453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |