Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,622,556 A→G 7.4% A394A (GCT→GCC)  PP_4941 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,622,5560AG 7.4% 151.9 / 2.7 54A394A (GCT→GCC) PP_4941hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/24);  new base G (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

CCCGGCAAGCCAGCGTTGCAGCAGGCGGCGCAGGCGGCGCTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAGGCTTCCAATACCGCC  >  NC_002947/5622418‑5622703
                                                                                                                                          |                                                                                                                                                   
cccGGCAAGCCAGCGTTGCAGCAGGCGGCGCAGGCGGCGCTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAg                                                                                                                                           <  6:414392/149‑1 (MQ=255)
   ggCAAGCCAGCGTTGCAGCAGGCGGCGCAGGCGGCGCTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAgcgc                                                                                                                                        >  7:194395/1‑149 (MQ=255)
     cAAGCCAGCGTTGCAGCAGGCGGCGCAGGCGGCGCTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTc                                                                                                                                      <  3:109345/149‑1 (MQ=255)
         ccAGCGTTGCAGCAGGCGGCGCAGGCGGCGCTTGAACGGTGCCGGCGGCTGGAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAAtt                                                                                                                                  >  6:242065/1‑149 (MQ=255)
               ttGCAGCAGGCGGCGCAGGCGGCGCTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTgcggcg                                                                                                                            <  2:399244/149‑1 (MQ=255)
                  cagcagGCGGCGCAGGCGGCGCTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGt                                                                                                                          >  6:28664/1‑148 (MQ=255)
                    gcaggcggcgcaggcggcGCTTGAACGGTGCCGGCGGCTGGAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTaaa                                                                                                                       <  5:242065/149‑1 (MQ=255)
                       ggcggcgcaggcggcgcTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGcc                                                                                                                    >  2:109423/1‑149 (MQ=255)
                                 gcggcgcTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGc                                                                                                          <  4:237707/149‑1 (MQ=255)
                                       cTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCt                                                                                                                                       >  7:12560/1‑114 (MQ=255)
                                       cTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCt                                                                                                                                       <  8:12560/114‑1 (MQ=255)
                                                tGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCg                                                                                           <  4:365587/149‑1 (MQ=255)
                                                    ggcggcTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGc                                                                                         >  7:353876/1‑147 (MQ=255)
                                                       ggcTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCTGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCg                                                                                    <  2:281508/149‑1 (MQ=255)
                                                            cAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCTATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTc                                                                                 <  2:281815/147‑1 (MQ=255)
                                                              ggTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACACCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTa                                                                             >  6:424537/1‑149 (MQ=255)
                                                                   tGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACACCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTg                                                                        <  5:424537/149‑1 (MQ=255)
                                                                         atccccAGGGACATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTAGCCCAACGCCCAGCGGTCGTTATCGTGCAggtg                                                                  >  6:98695/4‑149 (MQ=255)
                                                                          tGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGAGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAggtgg                                                                 >  2:347010/1‑149 (MQ=255)
                                                                             ccAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTg                                                              >  6:415048/1‑149 (MQ=255)
                                                                             ccAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTg                                                              >  6:111313/1‑149 (MQ=255)
                                                                              cAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTgg                                                             >  4:173127/1‑149 (MQ=255)
                                                                                gggCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCg                                                           <  7:275582/149‑1 (MQ=255)
                                                                                 ggCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCg                                                           >  5:64325/1‑148 (MQ=255)
                                                                                  gCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCTGTCGTTATCGTGCAGGTGGTTGGCGTa                                                         <  8:194395/149‑1 (MQ=255)
                                                                                    cATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGcc                                                                                                                    <  6:422626/88‑1 (MQ=255)
                                                                                    cATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGcc                                                                                                                    >  5:422626/1‑88 (MQ=255)
                                                                                             gTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAgg                                              >  2:190476/1‑149 (MQ=255)
                                                                                              tCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCa                                                                      >  4:96902/1‑124 (MQ=255)
                                                                                              tCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCa                                                                      <  3:96902/124‑1 (MQ=255)
                                                                                                gcgcTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGttt                                           >  2:233568/1‑149 (MQ=255)
                                                                                                 cgcTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGtt                                                               >  2:114086/1‑128 (MQ=255)
                                                                                                 cgcTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGtt                                                               <  1:114086/128‑1 (MQ=255)
                                                                                                 cgcTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTtg                                          <  6:64325/149‑1 (MQ=255)
                                                                                                           cgcAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCtgccat                                <  3:319877/149‑1 (MQ=255)
                                                                                                                     cACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATc                                                                           <  4:361992/96‑1 (MQ=255)
                                                                                                                     cACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATc                                                                           >  3:361992/1‑96 (MQ=255)
                                                                                                                        cAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGc                    >  4:181693/1‑148 (MQ=255)
                                                                                                                          ggCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCAGGCCATCGCCACTGTCGCCCATCGGCCAGCGGGCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAg                 <  6:132380/149‑1 (MQ=255)
                                                                                                                          ggCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTgg                                       >  1:176314/1‑127 (MQ=255)
                                                                                                                          ggCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTgg                                       <  2:176314/127‑1 (MQ=255)
                                                                                                                            cTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAGGc               <  5:415048/149‑1 (MQ=255)
                                                                                                                                 cgctGCCCAGGCTTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAACAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAg                 >  7:139952/4‑142 (MQ=255)
                                                                                                                                 cgctGCCCAGGCTTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAACAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAg                 <  8:139952/139‑1 (MQ=255)
                                                                                                                                  ggTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAGGCTTCCaa         >  7:67637/1‑149 (MQ=255)
                                                                                                                                  ggTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCCTGCAAGGCTTCCaa         >  1:256420/1‑149 (MQ=255)
                                                                                                                                   ctGCCCAGGCTTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAg                 <  2:27827/139‑1 (MQ=255)
                                                                                                                                   ctGCCCAGGCTTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAg                 >  1:27827/2‑140 (MQ=255)
                                                                                                                                    tGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAGGCTTCCAATa       <  1:233568/149‑1 (MQ=255)
                                                                                                                                      ctgcaGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAGGCTTCCAATAcc     <  6:385877/145‑1 (MQ=255)
                                                                                                                                      cccAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGc                                                                       <  6:144914/83‑1 (MQ=255)
                                                                                                                                      cccAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGc                                                                       >  5:144914/1‑83 (MQ=255)
                                                                                                                                      cccAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAgg                <  3:388684/138‑1 (MQ=255)
                                                                                                                                      cccAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAgg                >  4:388684/1‑138 (MQ=255)
                                                                                                                                       ccAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCCAGGCTTCCACTAccg    >  4:150822/1‑149 (MQ=255)
                                                                                                                                         aaGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAGGCTTCCAATAccgcc  >  5:192730/1‑149 (MQ=255)
                                                                                                                                         aaGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAGGCTTCCAATAccgcc  >  1:207761/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
CCCGGCAAGCCAGCGTTGCAGCAGGCGGCGCAGGCGGCGCTTGAACGGTGCCGGCGGCTGCAGGTCGTGCATCATGCCCAGGGCCATCGCCTTGTCGCGCTGGATCGCGCAGTCCAGCACCAGGCTGCAGGGTGCCCAAGCCTGGGCAGCGCTCAATTGCGGCGGTAAAGCCACGCCATCGCCACTGTCGCCCATCGGCCAGCGGTCGTTATCGTGCAGGTGGTTGGCGTAGCACAGCAAGGTTTGTGGCTGCCATGCCATGCCTTGCAAGGCTTCCAATACCGCC  >  NC_002947/5622418‑5622703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑