| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,655,636 | C→A | 13.7% | intergenic (‑91/+61) | epd ← / ← tktA | D‑erythrose 4‑phosphate dehydrogenase/transketolase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,655,636 | 0 | C | A | 13.7% | 82.0 / 7.1 | 43 | intergenic (‑91/+61) | epd/tktA | D‑erythrose 4‑phosphate dehydrogenase/transketolase A |
| Reads supporting (aligned to +/- strand): ref base C (17/19); new base A (3/3); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01 | |||||||||||
CACAGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCC > NC_002947/5655488‑5655781 | cacaGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGc < 1:370462/149‑1 (MQ=255) ggCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑GAGGTTGCATGAAAccc < 2:220740/149‑2 (MQ=255) tccGATACGGCCATAACCGTTTAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACTAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCTGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACctct < 2:349800/148‑1 (MQ=255) gCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACCGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑GAGGTTGCATGAAAccct < 5:356724/149‑3 (MQ=38) cGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCtgt > 2:357382/1‑149 (MQ=255) aTACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTgg < 1:357382/149‑1 (MQ=255) gCCATAACCGTTGAGTTCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCg < 3:89881/149‑1 (MQ=255) aaCCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGTCATTCGCGGGCAAGCCCCCACCCACA‑G‑GGTTTGATGGAACCTCTGTGGGAGCGGGcgt > 5:28055/1‑147 (MQ=255) tGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAt < 7:192360/149‑1 (MQ=255) gCAATTTTGTTGTGACGGTTGGGCATTCGTGTCTAACTAGACAGTTCAATTGTCTTGAGCCTGTGTAATTTTCGGGGTGGCATTCCGGGGCAAGGCCTCTGCATCA‑G‑GGTTTCTTTCATTGTCTGTGTGTGCGGGCTTGCCCGCGAAt < 2:99468/148‑1 (MQ=255) cAACTTTGTAGGGACGGTGGGGCATTCGCGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGCGCAAGACCGCTACCAAA‑T‑GGTTACATTCAACCTATGTGTGTGCGGGCTTGCCAGCGAAtt > 1:99468/1‑147 (MQ=255) aaCTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATg > 7:272772/1‑147 (MQ=255) cTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATg > 8:212803/1‑145 (MQ=255) cTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCCGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATg < 7:212803/145‑1 (MQ=255) aGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGc > 8:376220/1‑130 (MQ=255) aGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGc < 7:376220/130‑1 (MQ=255) gggACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCtgtg > 8:164190/1‑116 (MQ=255) gggACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCtgtg < 7:164190/116‑1 (MQ=255) ggggCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAg < 8:272772/149‑1 (MQ=255) ggCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTGTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGa > 3:45099/1‑148 (MQ=255) gCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGa < 8:136199/108‑1 (MQ=255) gCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATc < 4:45099/149‑1 (MQ=255) gagaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGGGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTc < 1:389267/149‑1 (MQ=255) ttCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCa < 1:266883/136‑1 (MQ=255) ttCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCa > 2:266883/1‑136 (MQ=255) aaTTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACAccgcccgc > 4:57778/1‑119 (MQ=255) aaTTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACAccgcccgc < 3:57778/119‑1 (MQ=255) gCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTAcc > 1:322170/1‑149 (MQ=255) ccTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCcagc > 8:205708/1‑131 (MQ=255) ccTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCcagc < 7:205708/131‑1 (MQ=255) tGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATAAGTCTTCCAGCAGCTCTTAGGCAGTAACGAg > 4:312350/1‑149 (MQ=255) ggAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCg > 1:309520/1‑135 (MQ=255) ggAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCg < 2:309520/135‑1 (MQ=255) tttGCGGTGTGGCATTCGCGGGCAAGCCCCCACCCACATG‑GGTTACA‑GCAACCTCAGTGGGAGCGGGGTTGCCCGCCAATTCAAGCGCCACCACCCCCACATCAGTAAATAAGTCTTCAAGAACCTCTTAGGCCGATACGAGGATGTtc > 8:355181/1‑149 (MQ=255) cATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAg < 3:296921/149‑1 (MQ=255) aGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCg < 7:355181/149‑1 (MQ=255) ccGCTCCCACA‑GAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGaa > 3:66083/1‑148 (MQ=37) cTCCCACA‑GAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAAt < 4:66083/149‑1 (MQ=38) ccACA‑G‑GGTTTCATGCAACCTATGTGGGAGCGCGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATAAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGAAGATCTCCAGGGTGAAGCAAAACTCATCGAACAATGcc > 4:331729/1‑149 (MQ=255) cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGcc > 1:152224/7‑148 (MQ=38) aca‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCg > 4:149701/1‑148 (MQ=255) ggTTTCATGCAACCTCTGTGGGAGGGGGCTTGCCCGCGAATGAAGGCGACACCGCCCTCAGATCttt < 6:131978/67‑1 (MQ=38) ggTTTCATGAAACCTCTGTGGGAGCGGGCAAGCCCGCGAATGAAGGCGACACCGCCCGCAGATCttt > 5:131978/1‑67 (MQ=37) aTGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGcc > 4:205476/1‑149 (MQ=255) | CACAGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACA‑G‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCC > NC_002947/5655488‑5655781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |