Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,725,652 C→G 6.0% P438P (CCG→CCC)  opgH ← glucans biosynthesis glucosyltransferase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,725,6520CG 6.0% 271.5 / 7.2 83P438P (CCG→CCC) opgHglucans biosynthesis glucosyltransferase H
Reads supporting (aligned to +/- strand):  ref base C (35/43);  new base G (2/3);  total (37/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GAGCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGC  >  NC_002947/5725508‑5725800
                                                                                                                                                |                                                                                                                                                    
gAGCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGc                                                                                                                                                   <  2:175899/148‑1 (MQ=255)
  gCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAAc                                                                                                                                                >  4:237605/1‑149 (MQ=255)
      gTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcg                                                                                                                                            <  4:18366/149‑1 (MQ=255)
      gTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcg                                                                                                                                            >  4:34442/1‑149 (MQ=255)
       tCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcgc                                                                                                                                           <  3:34442/149‑1 (MQ=255)
           gCAGGTTTGGCGGCAGTTCTTCGTAGCTGCTCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAgg                                                                                                                                       <  7:292719/149‑1 (MQ=255)
           gCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAgg                                                                                                                                       >  1:390853/1‑149 (MQ=255)
           gCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAgg                                                                                                                                       <  4:9264/149‑1 (MQ=255)
           gCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAgg                                                                                                                                       >  4:92229/1‑149 (MQ=255)
           gCAGGTTTGGCGGCAGTTCTTCGTAGATGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAgg                                                                                                                                       >  4:108997/1‑149 (MQ=255)
            cAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCg                                                                                                                                                     >  6:72444/1‑134 (MQ=255)
            cAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCg                                                                                                                                                     <  5:72444/134‑1 (MQ=255)
               gTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGgcgc                                                                                                                                   <  8:195357/149‑1 (MQ=255)
                tttGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCa                                                                                                                                  <  3:92229/149‑1 (MQ=255)
                tttGGCGGCAGTTCATCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCa                                                                                                                                  <  6:126420/149‑1 (MQ=255)
                  tGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAg                                                                                                                                 <  3:237605/148‑1 (MQ=255)
                    gcggcAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGc                                                                                                                              >  5:381145/1‑149 (MQ=255)
                         aGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                         <  6:95490/149‑1 (MQ=255)
                              ttcGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGa                                                                                                                          >  7:84260/1‑143 (MQ=255)
                              ttcGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGa                                                                                                                          <  8:84260/143‑1 (MQ=255)
                                cGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAAc                                                                                                                     <  3:148412/146‑1 (MQ=255)
                                       gCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGc                                                                                                           >  6:57878/1‑149 (MQ=255)
                                        cccGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATg                                                                                                            <  4:396326/147‑1 (MQ=255)
                                          cGGCAAGTCGTAGGCAATCCACACACCCGAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGTCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGga                                                                                                        <  8:121471/149‑1 (MQ=255)
                                          cGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGga                                                                                                        <  6:381145/149‑1 (MQ=255)
                                            gCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGgat                                                                                                       >  8:327070/1‑148 (MQ=255)
                                            gCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGga                                                                                                        <  3:170842/147‑1 (MQ=255)
                                            gCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGga                                                                                                        >  4:170842/1‑147 (MQ=255)
                                                gTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGACTTCATGCGGATGATcg                                                                                                  <  4:114076/149‑1 (MQ=255)
                                                 tCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                         >  8:209457/1‑125 (MQ=255)
                                                 tCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                         <  7:209457/125‑1 (MQ=255)
                                                 tCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATcgc                                                                                                 >  4:358680/1‑149 (MQ=255)
                                                    tAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGtt                                                                                              >  3:296039/1‑149 (MQ=255)
                                                      ggCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGTCGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTtgt                                                                                            <  1:207270/149‑1 (MQ=255)
                                                      ggCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTtg                                                                                             <  5:57878/148‑1 (MQ=255)
                                                       gCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTtgtg                                                                                           <  4:137553/149‑1 (MQ=255)
                                                        cAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATc                                                                                                   >  1:224910/1‑140 (MQ=255)
                                                        cAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATc                                                                                                   <  2:224910/140‑1 (MQ=255)
                                                         aaTCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATcgcg                                                                                                <  6:48926/142‑1 (MQ=255)
                                                         aaTCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATcgcg                                                                                                >  5:48926/1‑142 (MQ=255)
                                                          aTCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGGAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGcc                                                                                        <  1:14505/149‑1 (MQ=255)
                                                           tCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                                       >  8:71757/1‑149 (MQ=255)
                                                             cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                         >  3:296181/1‑113 (MQ=255)
                                                             cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                         <  4:296181/113‑1 (MQ=255)
                                                             cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATgg                                                                                                                       >  1:402837/1‑114 (MQ=255)
                                                             cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATgg                                                                                                                       <  2:402837/115‑2 (MQ=255)
                                                             cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                                       >  8:109671/1‑147 (MQ=255)
                                                             cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                                       >  3:402117/1‑147 (MQ=255)
                                                             cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCa                                                                                     <  4:296039/149‑1 (MQ=255)
                                                              acacacCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcgcgttc                                                                                                                                       >  6:169905/1‑94 (MQ=38)
                                                              acacacCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcgcgttc                                                                                                                                       <  5:169905/98‑5 (MQ=38)
                                                                acacCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCagta                                                                                  <  7:109671/149‑1 (MQ=255)
                                                                    cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCAt                                                                                                             >  2:38608/1‑118 (MQ=255)
                                                                    cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCAt                                                                                                             <  1:38608/118‑1 (MQ=255)
                                                                    cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGc                                                                              >  7:371771/1‑149 (MQ=255)
                                                                    cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGc                                                                              >  6:328557/1‑149 (MQ=255)
                                                                      cAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGa                                                                            <  6:63368/149‑1 (MQ=255)
                                                                      cAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGa                                                                            <  6:295422/149‑1 (MQ=255)
                                                                       aGCCGGCACGGCGCATCAGCGCAGCTTCGACGAGGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTgg                                                                                          >  1:343011/1‑134 (MQ=255)
                                                                       aGCCGGCACGGCGCATCAGCGCAGCTTCGACGAGGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTgg                                                                                          <  2:343011/134‑1 (MQ=255)
                                                                          cGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGtt                                                                                              <  4:259707/127‑1 (MQ=255)
                                                                          cGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGtt                                                                                              >  3:259707/1‑127 (MQ=255)
                                                                           ggCACGGCGCATCAGCGCATCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGTGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCAc                                                                       <  3:108997/149‑1 (MQ=255)
                                                                            gCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCAcc                                                                      >  5:387909/1‑149 (MQ=255)
                                                                                         gcgcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGaa                                                          <  4:402117/148‑1 (MQ=255)
                                                                                         gcgcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAg                                                         <  6:387909/149‑1 (MQ=255)
                                                                                          cgcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGt                                                        <  5:267685/149‑1 (MQ=255)
                                                                                           gcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCAcc                                                                      <  6:30994/134‑1 (MQ=255)
                                                                                           gcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCAcc                                                                      >  5:30994/1‑134 (MQ=255)
                                                                                            cAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTg                                                       >  4:115309/1‑148 (MQ=255)
                                                                                            cAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGc                                                      <  3:358680/149‑1 (MQ=255)
                                                                                                   gacgaAGACGTGGCAAAGGATGGCACCGGCGAACGCGCATTTCCCCGGCAACGGCCACAGGGCGGAGTGCTAGAGGAACGGCGTCATGCGGATGATCGCTTTGTGCCCCCAGTAGAGCGACTCACCCAACTGCGAGAAGTGCAGCCCgg                                               >  8:273339/1‑149 (MQ=255)
                                                                                                            gTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACa                                      <  2:333804/149‑1 (MQ=255)
                                                                                                              gggAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                                       >  1:392975/1‑98 (MQ=255)
                                                                                                              gggAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                                       <  2:392975/98‑1 (MQ=255)
                                                                                                               ggAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTg                                   <  5:328557/149‑1 (MQ=255)
                                                                                                               ggAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGACAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTg                                   >  5:329994/1‑149 (MQ=255)
                                                                                                                gAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTgg                                  >  7:126273/1‑149 (MQ=255)
                                                                                                                              tgCGAACGCGCCTTTGACCGGCAACGGCGCCAGGGATCAGTGCTCGATGAACGGCTTCATGCGGATTATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGACa                                                             <  4:331005/107‑1 (MQ=255)
                                                                                                                              ggCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTGCATGCGGATGATCGCGGTGTGGCCCCAGTAGTGCGACTCCCCCAACTGCCa                                                             >  3:331005/1‑108 (MQ=255)
                                                                                                                                      cgcCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAAc            >  7:105173/1‑149 (MQ=255)
                                                                                                                                       gcCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAAct           >  4:331751/1‑149 (MQ=255)
                                                                                                                                         cTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAActgc         <  7:71757/149‑1 (MQ=255)
                                                                                                                                              cccGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCgg                     >  6:141688/1‑132 (MQ=255)
                                                                                                                                              cccGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCgg                     <  5:141688/132‑1 (MQ=255)
                                                                                                                                              cccGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCAt    <  6:329994/149‑1 (MQ=255)
                                                                                                                                                cGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGc  <  8:105173/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GAGCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGC  >  NC_002947/5725508‑5725800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑