Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,725,658 C→A 5.9% P436P (CCG→CCT)  opgH ← glucans biosynthesis glucosyltransferase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,725,6580CA 5.9% 277.9 / 6.2 85P436P (CCG→CCT) opgHglucans biosynthesis glucosyltransferase H
Reads supporting (aligned to +/- strand):  ref base C (35/45);  new base A (2/3);  total (37/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01

GCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTG  >  NC_002947/5725510‑5725803
                                                                                                                                                    |                                                                                                                                                 
gCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAAc                                                                                                                                                   >  4:237605/1‑149 (MQ=255)
    gTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcg                                                                                                                                               <  4:18366/149‑1 (MQ=255)
    gTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcg                                                                                                                                               >  4:34442/1‑149 (MQ=255)
     tCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcgc                                                                                                                                              <  3:34442/149‑1 (MQ=255)
         gCAGGTTTGGCGGCAGTTCTTCGTAGCTGCTCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAgg                                                                                                                                          <  7:292719/149‑1 (MQ=255)
         gCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAgg                                                                                                                                          >  1:390853/1‑149 (MQ=255)
         gCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAgg                                                                                                                                          <  4:9264/149‑1 (MQ=255)
         gCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAgg                                                                                                                                          >  4:92229/1‑149 (MQ=255)
         gCAGGTTTGGCGGCAGTTCTTCGTAGATGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAgg                                                                                                                                          >  4:108997/1‑149 (MQ=255)
             gTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGgcgc                                                                                                                                      <  8:195357/149‑1 (MQ=255)
              tttGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCa                                                                                                                                     <  3:92229/149‑1 (MQ=255)
              tttGGCGGCAGTTCATCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCa                                                                                                                                     <  6:126420/149‑1 (MQ=255)
                tGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAg                                                                                                                                    <  3:237605/148‑1 (MQ=255)
                  gcggcAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGc                                                                                                                                 >  5:381145/1‑149 (MQ=255)
                       aGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                            <  6:95490/149‑1 (MQ=255)
                            ttcGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGa                                                                                                                             >  7:84260/1‑143 (MQ=255)
                            ttcGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGa                                                                                                                             <  8:84260/143‑1 (MQ=255)
                              cGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAAc                                                                                                                        <  3:148412/146‑1 (MQ=255)
                                     gCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGc                                                                                                              >  6:57878/1‑149 (MQ=255)
                                      cccGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATg                                                                                                               <  4:396326/147‑1 (MQ=255)
                                        cGGCAAGTCGTAGGCAATCCACACACCCGAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGTCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGga                                                                                                           <  8:121471/149‑1 (MQ=255)
                                        cGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGga                                                                                                           <  6:381145/149‑1 (MQ=255)
                                          gCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGgat                                                                                                          >  8:327070/1‑148 (MQ=255)
                                          gCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGga                                                                                                           <  3:170842/147‑1 (MQ=255)
                                          gCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGga                                                                                                           >  4:170842/1‑147 (MQ=255)
                                              gTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGACTTCATGCGGATGATcg                                                                                                     <  4:114076/149‑1 (MQ=255)
                                               tCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                            >  8:209457/1‑125 (MQ=255)
                                               tCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                            <  7:209457/125‑1 (MQ=255)
                                               tCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATcgc                                                                                                    >  4:358680/1‑149 (MQ=255)
                                                  tAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGtt                                                                                                 >  3:296039/1‑149 (MQ=255)
                                                    ggCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGTCGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTtgt                                                                                               <  1:207270/149‑1 (MQ=255)
                                                    ggCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTtg                                                                                                <  5:57878/148‑1 (MQ=255)
                                                     gCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTtgtg                                                                                              <  4:137553/149‑1 (MQ=255)
                                                      cAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATc                                                                                                      >  1:224910/1‑140 (MQ=255)
                                                      cAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATc                                                                                                      <  2:224910/140‑1 (MQ=255)
                                                       aaTCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATcgcg                                                                                                   <  6:48926/142‑1 (MQ=255)
                                                       aaTCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATcgcg                                                                                                   >  5:48926/1‑142 (MQ=255)
                                                        aTCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGGAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGcc                                                                                           <  1:14505/149‑1 (MQ=255)
                                                         tCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                                          >  8:71757/1‑149 (MQ=255)
                                                           cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                            <  4:296181/113‑1 (MQ=255)
                                                           cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                            >  3:296181/1‑113 (MQ=255)
                                                           cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATgg                                                                                                                          <  2:402837/115‑2 (MQ=255)
                                                           cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATgg                                                                                                                          >  1:402837/1‑114 (MQ=255)
                                                           cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                                          >  8:109671/1‑147 (MQ=255)
                                                           cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                                          >  3:402117/1‑147 (MQ=255)
                                                           cacacaCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCa                                                                                        <  4:296039/149‑1 (MQ=255)
                                                            acacacCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcgcgttc                                                                                                                                          >  6:169905/1‑94 (MQ=38)
                                                            acacacCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcgcgttc                                                                                                                                          <  5:169905/98‑5 (MQ=38)
                                                              acacCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCagta                                                                                     <  7:109671/149‑1 (MQ=255)
                                                                  cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCAt                                                                                                                >  2:38608/1‑118 (MQ=255)
                                                                  cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCAt                                                                                                                <  1:38608/118‑1 (MQ=255)
                                                                  cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGc                                                                                 >  6:328557/1‑149 (MQ=255)
                                                                  cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGc                                                                                 >  7:371771/1‑149 (MQ=255)
                                                                    cAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGa                                                                               <  6:63368/149‑1 (MQ=255)
                                                                    cAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGa                                                                               <  6:295422/149‑1 (MQ=255)
                                                                     aGCCGGCACGGCGCATCAGCGCAGCTTCGACGAGGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTgg                                                                                             >  1:343011/1‑134 (MQ=255)
                                                                     aGCCGGCACGGCGCATCAGCGCAGCTTCGACGAGGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTgg                                                                                             <  2:343011/134‑1 (MQ=255)
                                                                        cGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGtt                                                                                                 >  3:259707/1‑127 (MQ=255)
                                                                        cGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGtt                                                                                                 <  4:259707/127‑1 (MQ=255)
                                                                         ggCACGGCGCATCAGCGCATCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGTGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCAc                                                                          <  3:108997/149‑1 (MQ=255)
                                                                          gCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCAcc                                                                         >  5:387909/1‑149 (MQ=255)
                                                                                       gcgcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGaa                                                             <  4:402117/148‑1 (MQ=255)
                                                                                       gcgcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAg                                                            <  6:387909/149‑1 (MQ=255)
                                                                                        cgcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGt                                                           <  5:267685/149‑1 (MQ=255)
                                                                                         gcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCAcc                                                                         <  6:30994/134‑1 (MQ=255)
                                                                                         gcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCAcc                                                                         >  5:30994/1‑134 (MQ=255)
                                                                                          cAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTg                                                          >  4:115309/1‑148 (MQ=255)
                                                                                          cAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGc                                                         <  3:358680/149‑1 (MQ=255)
                                                                                                 gacgaAGACGTGGCAAAGGATGGCACCGGCGAACGCGCATTTCCCCGGCAACGGCCACAGGGCGGAGTGCTAGAGGAACGGCGTCATGCGGATGATCGCTTTGTGCCCCCAGTAGAGCGACTCACCCAACTGCGAGAAGTGCAGCCCgg                                                  >  8:273339/1‑149 (MQ=255)
                                                                                                          gTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACa                                         <  2:333804/149‑1 (MQ=255)
                                                                                                            gggAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                                          <  2:392975/98‑1 (MQ=255)
                                                                                                            gggAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                                          >  1:392975/1‑98 (MQ=255)
                                                                                                             ggAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTg                                      <  5:328557/149‑1 (MQ=255)
                                                                                                             ggAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGACAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTg                                      >  5:329994/1‑149 (MQ=255)
                                                                                                              gAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTgg                                     >  7:126273/1‑149 (MQ=255)
                                                                                                                            tgCGAACGCGCCTTTGACCGGCAACGGCGCCAGGGATCAGTGCTCGATGAACGGCTTCATGCGGATTATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGACa                                                                <  4:331005/107‑1 (MQ=255)
                                                                                                                            ggCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTGCATGCGGATGATCGCGGTGTGGCCCCAGTAGTGCGACTCCCCCAACTGCCa                                                                >  3:331005/1‑108 (MQ=255)
                                                                                                                                    cgcCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAAc               >  7:105173/1‑149 (MQ=255)
                                                                                                                                     gcCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAAct              >  4:331751/1‑149 (MQ=255)
                                                                                                                                       cTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAActgc            <  7:71757/149‑1 (MQ=255)
                                                                                                                                            cccGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCgg                        <  5:141688/132‑1 (MQ=255)
                                                                                                                                            cccGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCgg                        >  6:141688/1‑132 (MQ=255)
                                                                                                                                            cccGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCAt       <  6:329994/149‑1 (MQ=255)
                                                                                                                                              cGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGc     <  8:105173/149‑1 (MQ=255)
                                                                                                                                                gCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGt   <  3:115309/149‑1 (MQ=255)
                                                                                                                                                  aaCGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTg  >  7:310754/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTG  >  NC_002947/5725510‑5725803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑