Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,734,000 G→A 6.2% intergenic (‑26/+131) proY ← / ← hutH proline (histidine) APC transporter/histidine ammonia‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,734,0000GA 6.2% 194.5 / 2.1 64intergenic (‑26/+131)proY/hutHproline (histidine) APC transporter/histidine ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base A (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

TCTGGATGGCCGAGGCAGAGCCGTAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGGCGCGCCCGCGAAGGGCCGCCCAGCGGCCCCAGGGGCGTTACAGGCTTG  >  NC_002947/5733860‑5734141
                                                                                                                                             |                                                                                                                                              
tCTGGATGGCCGAGGCAGAGCCGTAGAGAAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGt                                                                                                                                            >  1:12022/1‑146 (MQ=255)
  tGGATGGCCGAGGCAGAGCCGTAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGAt                                                                                                                                       >  7:38952/1‑148 (MQ=255)
       ggCCGAGGCAGAGCCGTAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAggg                                                                                                                                 >  7:369226/1‑149 (MQ=255)
        gCCGAGGCAGAGCCGTAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAgg                                                                                                                                  <  4:31823/147‑1 (MQ=255)
            aGGCAGAGCCGTAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGgctg                                                                                                                            >  1:204095/1‑149 (MQ=255)
                agagCCGTAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCtgctgc                                                                                                                        >  3:374962/1‑149 (MQ=255)
                     cGTAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGc                                                                                                                   <  2:12022/149‑1 (MQ=255)
                       tAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCg                                                                                                                                             >  4:386536/1‑121 (MQ=255)
                       tAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCg                                                                                                                                             <  3:386536/121‑1 (MQ=255)
                       tAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGt                                                                                                                                            <  1:310783/122‑1 (MQ=255)
                       tAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGt                                                                                                                                            >  2:310783/1‑122 (MQ=255)
                       tAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGccc                                                                                                                 <  5:349881/149‑1 (MQ=255)
                         gA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCt                                                                                                               >  4:271002/1‑149 (MQ=255)
                                ggcGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGTTGTTGCGGGCACTTAGCGCGCTCTTGAGTCCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTTTGGTCGTGAGATTCTGGGGGCTGCTGCGCAGGCCCTTCGCgg                                                                                                         <  4:179019/147‑1 (MQ=255)
                                gCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCgg                                                                                                         <  8:369226/149‑1 (MQ=255)
                                   ggTGCAGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCa                                                                                                      <  4:382151/149‑1 (MQ=255)
                                     tGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGACCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCaag                                                                                                    >  3:144911/1‑147 (MQ=255)
                                         gATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCGTGCCCg                                                                                                >  8:140766/1‑149 (MQ=255)
                                             gCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCGTGCCCGcga                                                                                             <  4:324307/148‑3 (MQ=255)
                                               ggAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGGCCGcccccggccc                                                                                          >  3:115416/1‑142 (MQ=255)
                                               ggAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCGTGCCCGcgaagg                                                                                          <  7:140766/149‑6 (MQ=255)
                                                    cGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAggg                                                                                     >  8:265620/1‑149 (MQ=255)
                                                        ggCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATg                                                                                 <  1:367078/149‑1 (MQ=255)
                                                          ccATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGGATGGCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCTCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGgt                                                                               <  5:244603/149‑1 (MQ=255)
                                                          ccATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCa                                                                                                                     <  3:210855/111‑1 (MQ=255)
                                                          ccATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCa                                                                                                                     >  4:210855/1‑111 (MQ=255)
                                                          ccATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGgt                                                                               >  2:136133/1‑149 (MQ=255)
                                                           cATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGc                                                                                                                   >  8:196826/1‑112 (MQ=255)
                                                               aaaCGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑ttt                                                                          <  8:38952/148‑1 (MQ=255)
                                                                  cgtttgtgGCGGGCAGTTACCCGGCGCTGGTTAACTTGTGCTTGTTGCTTGTAACGTCCTTTAATTGTTTTTGGGGGCGTGACATTCCGGGGGGTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCa                                                                      <  2:204095/145‑1 (MQ=255)
                                                                   ggATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCAc                                                                     <  6:31979/149‑1 (MQ=255)
                                                                    gATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTga                                                                                                                                          <  6:170033/80‑1 (MQ=255)
                                                                    gATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTga                                                                                                                                          >  5:170033/1‑80 (MQ=255)
                                                                            gggCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGcc                                                                                                  <  6:65220/112‑1 (MQ=255)
                                                                            gggCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGcc                                                                                                  >  5:65220/1‑112 (MQ=255)
                                                                            gggCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAgaagt                                                                                   <  2:82433/127‑5 (MQ=255)
                                                                            gggCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAgaagt                                                                                   >  1:82433/1‑123 (MQ=255)
                                                                                aCTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTgg                                                         <  4:144911/148‑1 (MQ=255)
                                                                                aCTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCCCTCCCACAGGGGATGGTG‑TTTTTGACCAACTTATGTggg                                                        >  1:214284/1‑149 (MQ=255)
                                                                                 cTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGATCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTgg                                                         >  3:72717/1‑147 (MQ=255)
                                                                                 cTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGATCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTgg                                                         >  3:78315/1‑147 (MQ=255)
                                                                                  ttAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGATCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAg                                                      <  4:78315/149‑1 (MQ=255)
                                                                                  ttAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGATCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAg                                                      <  4:72717/149‑1 (MQ=255)
                                                                                     gCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGa                                                       >  8:294167/1‑145 (MQ=255)
                                                                                     gCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGa                                                       <  7:294167/145‑1 (MQ=255)
                                                                                        cgcgCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGGACCCCCCCCCCCCCACGGGCTGGGGTTTTTGCGCAT‑CTTCTTTGGGGGCggggg                                                >  6:46883/1‑147 (MQ=255)
                                                                                         gcgcTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGgcgc                                               <  4:340866/149‑1 (MQ=255)
                                                                                             ttGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCCCCCCCCCTACCACACGGGATGGTG‑TTTTTGACCAACATCTGTGGGAGCGGGCGCGccc                                           >  1:164401/1‑149 (MQ=255)
                                                                                               gagaCCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGgcggcccttc                                         >  3:70044/1‑142 (MQ=255)
                                                                                                   ccTTGCGGTTGTTGAATGCCACTGCCTTGAATTGTTTTTGTGGTGGGTAGTTTCTGGGGGCGGGTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACTGGGGTTGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGGCGCGCCCGCGAAg                                     <  4:115416/149‑1 (MQ=255)
                                                                                                              ttgCTTGTCAGGGCTTTGATTTTTTTTTGTGGGCGTGATATTCTGGGGGCTGCGGCGCAGCCCCTTCGCGGGCACGCCGGCTCCCACCGGGGATGGTG‑TTTTGCACCTACTTTTGTGGTCGGGGGCGCGCCCGCGAAGGGCCGCCCAg                           <  2:346763/148‑1 (MQ=255)
                                                                                                                  aTGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCtg                                                            <  3:308451/111‑1 (MQ=255)
                                                                                                                  aTGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCtg                                                            >  4:308451/1‑111 (MQ=255)
                                                                                                                       aCGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGGCGCGCCCGCGAAGGGCCGCCCAGCGGCCCCAgg                 >  8:309869/1‑149 (MQ=255)
                                                                                                                             ttGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCTACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGGCGCGCCCGCGAAGGGCCGCCCAGCCGCCCCAGGGGCGt            >  7:228946/1‑148 (MQ=255)
                                                                                                                             ttGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTTGACCTACTTCTGTGGGATCGGGCGCGCCCGCGAAGGGCCGCCCAGCGGCCCCAGGGGCGt            >  7:176176/1‑148 (MQ=255)
                                                                                                                              tGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGgc                                                 <  2:302021/110‑1 (MQ=255)
                                                                                                                              tGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGgc                                                 >  1:302021/1‑110 (MQ=255)
                                                                                                                                aaTTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTAc                                                                 >  6:371283/1‑92 (MQ=255)
                                                                                                                                aaTTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTAc                                                                 <  5:371283/92‑1 (MQ=255)
                                                                                                                                 aTTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCGCCCACAGGGGAGGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGGCGCGCCCGCGAAGGGCCGCCCAGCGGCCCCAGGGGCGTTACAg       <  7:265620/149‑1 (MQ=255)
                                                                                                                                  ttGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGGCGCGCCCGCGAAGGGCCGCCCAGCGGCCCCAGGGGCGTTACAgg      >  5:76536/1‑149 (MQ=255)
                                                                                                                                  ttGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGGCGCGCCCGCGAAGGGCCGCCCAGCGGCCCCAGGGGCGTTACAgg      >  2:234558/1‑149 (MQ=255)
                                                                                                                                       tttGTGGTCTTCAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACCGGGGATGGTG‑TGTTGCACCTTCTTCTGTGGTAGCGGGCGCGCCCGCGAAGGGCCGCCCAGCGGCCCCAGGGGCGTTAGAGGCTTg  <  5:215576/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                              
TCTGGATGGCCGAGGCAGAGCCGTAGA‑AAAGGCCGGTGCCGATGGCGGAGCCGAGGGCCATGAAACGGATGTGGCGGGCACTTAGCCCGCGCTTGAGACCTTGAGCTTGTTGCATGTCACGTCCTTGAATTGTTTTTGTGGTCGTGAGATTCAGGGGGCTGCTGCGCAGCCCCTTCGCGGGCACGCCCGCTCCCACAGGGGATGGTG‑TTTTGCACCTACTTCTGTGGGAGCGGGCGCGCCCGCGAAGGGCCGCCCAGCGGCCCCAGGGGCGTTACAGGCTTG  >  NC_002947/5733860‑5734141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑