Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,740,467 T→G 17.9% intergenic (+32/+39) hutF → / ← PP_5037 formiminoglutamate deiminase/lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,740,4670TG17.9% 97.6 / 14.9 50intergenic (+32/+39)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (13/28);  new base G (4/5);  total (17/33)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCA  >  NC_002947/5740321‑5740615
                                                                                                                                                  |                                                                                                                                                    
ccAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGGGc                                                                                                                                                    >  7:218701/1‑149 (MQ=255)
            tgtgATGGTGAATGGGCAATGGGTGGTGCGGCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAATGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCTGGCTTGCACGCGAAg                                                                                                                                        <  3:158928/149‑1 (MQ=38)
            tgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCTGGCTTGCACGCGAAg                                                                                                                                        <  6:357728/149‑1 (MQ=39)
            ggtgATGGTGAATGGGCAATGGGCGGGGCGCCAGGGGCGGCATGCCCAGGAAGCTGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAg                                                                                                                                        <  7:154849/148‑1 (MQ=255)
              tgATGGTGAATGGGCAATGGGTGGTGCGCCATGGGCTGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAgg                                                                                                                                       <  6:39482/148‑1 (MQ=255)
              tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGGGCTTGCACGCGAAggg                                                                                                                                      <  8:218701/149‑1 (MQ=255)
                          ggCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgcaacgcaa                                                                                                                           <  5:215708/148‑1 (MQ=255)
                           gCATTGGGGGGGGCGCCTGGGGGGGCTTGCCCTGTAAGATTAACGGGGGGGGGCGTTTGCCATGGTGTTGCGCCAGCTTTTGGGCTGAGCTTACGGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCTATGGGTTGAAACGCAAcc                                                                                                                         <  6:21033/149‑1 (MQ=255)
                                     gtgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACt                                                                                                               <  1:59239/149‑1 (MQ=255)
                                          ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTGAGCCCTTAGGGGGGAAGCCCGCCCCGGGTTTGACAGCAACCCCAAAAAACAAAGTg                                                                                                          >  3:86000/1‑149 (MQ=255)
                                          ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGccc                                                                                                                                              <  8:61217/113‑1 (MQ=255)
                                          ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGccc                                                                                                                                              >  7:61217/1‑113 (MQ=255)
                                               ggcggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGGGTTTAAGCCCATAGCCTGCCAACCCCCGCAGGGTTGGCACCCACACCCAAAAAACACACTggaatt                                                                                                     >  2:23861/1‑144 (MQ=255)
                                                         cctggAACATGAACGCGGGCGGGGGTTTGCCACGGTGTTGCGCCTGCTTTTGGGCTGCGCGTACGGGGGTTGCGTTCCAGCCCTTCGCGGGCAACCCCGCTATGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTg                                                                                           <  4:263631/146‑1 (MQ=255)
                                                          cAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGc                                                                                          <  5:331187/149‑1 (MQ=255)
                                                              aaGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGAt                                                                                      <  3:375712/149‑1 (MQ=255)
                                                               aGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATc                                                                                     <  5:373415/149‑1 (MQ=255)
                                                                    aaaGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCTGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAc                                                                                 <  6:85409/148‑1 (MQ=255)
                                                                     aaGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCATAGCGTGCAAGCCCGCGAAGGGTTGGAACGCAACCCCAAAAAAATCAGTGCATTTTTTCTATTTGGTGATCGGgcgc                                                                               >  4:100835/1‑149 (MQ=255)
                                                                                  tttGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGTTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAg                                                                  <  8:48033/149‑1 (MQ=255)
                                                                                        aaGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTg                                                            <  3:100835/149‑1 (MQ=255)
                                                                                        aaGGTGTGGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCTAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTg                                                            <  1:23861/149‑1 (MQ=255)
                                                                                              ttGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGGGTTGCAGCCCTTCGCGTGCAAGCCCGCTAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTc                                                       <  4:369237/148‑1 (MQ=255)
                                                                                              ttGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTc                                                       <  5:220982/148‑1 (MQ=255)
                                                                                                   ccAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAccc                                                 <  3:372693/149‑1 (MQ=255)
                                                                                                            gggCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCTATGGTTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc                                         <  1:128431/148‑1 (MQ=255)
                                                                                                                  cccGTACCGGGGTTGCGTTCCAGCCCTTCGCGTGCAAGCCCGCTAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                              <  7:374996/119‑1 (MQ=255)
                                                                                                                         cGGGGGTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCTAAGGTTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCTCTGCTGCCGCGCCCTGGAcagc                           <  7:62732/149‑1 (MQ=255)
                                                                                                                          ggggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                          <  5:185450/149‑1 (MQ=255)
                                                                                                                           gggTTGCGTTGCAGCCCTTAGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGGATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAccgcag                         >  1:326095/1‑149 (MQ=255)
                                                                                                                              ttgcgttgcAGCCCTTCGCGTGCACGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgcg                                      <  8:310680/133‑1 (MQ=255)
                                                                                                                              ttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgcg                                      >  7:310680/1‑133 (MQ=255)
                                                                                                                                 cgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc                   <  2:45558/149‑1 (MQ=255)
                                                                                                                                  gttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                  <  7:16377/149‑1 (MQ=255)
                                                                                                                                  gttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                  <  6:128095/149‑1 (MQ=255)
                                                                                                                                   ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                 >  8:128984/1‑149 (MQ=255)
                                                                                                                                   ttgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                 >  1:392085/1‑149 (MQ=38)
                                                                                                                                    tgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTg                                                                                           >  2:309824/1‑74 (MQ=14)
                                                                                                                                    tgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTg                                                                                           <  1:309824/74‑1 (MQ=14)
                                                                                                                                       aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc             >  7:386265/1‑149 (MQ=255)
                                                                                                                                         cccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc            >  2:29808/1‑148 (MQ=255)
                                                                                                                                         cccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc            >  3:129491/1‑148 (MQ=255)
                                                                                                                                           cTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGTATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                 <  7:339726/141‑1 (MQ=255)
                                                                                                                                           cTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGTATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                 >  8:339726/1‑141 (MQ=255)
                                                                                                                                            ttCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc            >  5:61573/1‑145 (MQ=255)
                                                                                                                                            ttCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc            <  6:61573/145‑1 (MQ=255)
                                                                                                                                            ttCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAgg        >  2:349282/1‑149 (MQ=255)
                                                                                                                                              cgcgTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGcc      >  1:263809/1‑149 (MQ=255)
                                                                                                                                              cgccTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTACCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAgg        <  5:198379/147‑1 (MQ=255)
                                                                                                                                               gcgTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGcc      <  7:128984/148‑1 (MQ=255)
                                                                                                                                                  tGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCa  >  5:344904/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCA  >  NC_002947/5740321‑5740615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑