Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,777,403 A→C 6.4% intergenic (+22/+21) betA‑II → / ← PP_5065 choline dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,777,4030AC 6.4% 195.1 / 3.0 62intergenic (+22/+21)betA‑II/PP_5065choline dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/31);  new base C (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

CCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCTGTGCGCATTTCACGCACGGCCTTCCCGCCTGGCGCCT  >  NC_002947/5777260‑5777551
                                                                                                                                               |                                                                                                                                                    
ccGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGc                                                                                                                                                 <  8:158880/149‑1 (MQ=255)
      agaTTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCttt                                                                                                                                           <  7:281127/149‑1 (MQ=255)
       gaTTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTc                                                                                                                                          <  7:331669/149‑1 (MQ=255)
         tttCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCTGCGCGGCCCCt                                                                                                                                             <  4:296008/144‑1 (MQ=255)
         tttCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCTGCGCGGCCCCt                                                                                                                                             >  3:296008/1‑144 (MQ=255)
             ggACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGttt                                                                                                                                    <  5:26239/149‑1 (MQ=255)
                 aaGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCt                                                                                                                                 >  6:215445/1‑148 (MQ=255)
                        gTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCTGCGCGGCCCCtgatc                                                                                                                                         >  7:226709/1‑129 (MQ=255)
                        gTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCTGCGCGGCCCCtgatc                                                                                                                                         <  8:226709/133‑5 (MQ=255)
                        gTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCggg                                                                                                                         >  5:251055/1‑149 (MQ=255)
                               cAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAgcgc                                                                                                                   >  6:146704/1‑148 (MQ=255)
                                     aCTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAAt                                                                                                            <  6:251055/149‑1 (MQ=255)
                                     aCTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAAt                                                                                                            <  2:268467/149‑1 (MQ=255)
                                         ccGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTgc                                                                                                        >  3:265973/1‑149 (MQ=255)
                                         ccGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTgc                                                                                                        <  3:293705/149‑1 (MQ=255)
                                         ccGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTgc                                                                                                        <  7:56255/149‑1 (MQ=255)
                                          cgcgCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTgcg                                                                                                       >  2:278096/1‑149 (MQ=255)
                                           gcgcAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTgcgc                                                                                                      <  5:215445/149‑1 (MQ=255)
                                               agcaCCGCCAAGTACTACGTGGC‑GGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCaa                                                                                                 >  4:268560/1‑149 (MQ=255)
                                                 caCCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAAc                                                                                                >  8:232688/1‑149 (MQ=255)
                                                   ccGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACg                                                                                               >  4:199858/1‑148 (MQ=255)
                                                   ccGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGa                                                                                              <  5:146704/149‑1 (MQ=255)
                                                    cGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGaa                                                                                             <  4:265973/149‑1 (MQ=255)
                                                      ccAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAat                                                                                            >  3:93987/1‑148 (MQ=255)
                                                       cAAGGACTACGTGGCGGGGGAAGCACCAGTGAAAGGCAAGCCGATGCTTGAACGGAAACACGGCTGAGGCCTTGAGCAGTGGCCGCCGAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGGGCAAAAATTTTGCGCATCAACGAatc                                                                                           <  8:343470/148‑1 (MQ=255)
                                                             ctacGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATTCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTc                                                                                     <  7:331349/148‑1 (MQ=255)
                                                             ctacGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTc                                                                                     <  7:232688/148‑1 (MQ=255)
                                                               acGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTg                                                                                                                                   <  6:78298/100‑1 (MQ=255)
                                                               acGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTg                                                                                                                                   >  5:78298/1‑100 (MQ=255)
                                                                cGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGaca                                                                                 >  7:75044/1‑149 (MQ=255)
                                                                cGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGac                                                                                  <  6:342271/148‑1 (MQ=255)
                                                                cGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGa                                                                                   <  2:67812/147‑1 (MQ=255)
                                                                      gggggATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTc                                                                                                                                          >  5:115773/1‑86 (MQ=255)
                                                                      gggggATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTc                                                                                                                                          <  6:115773/86‑1 (MQ=255)
                                                                               aCCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTc                                                                                     >  6:215882/1‑130 (MQ=255)
                                                                               aCCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTc                                                                                     <  5:215882/130‑1 (MQ=255)
                                                                               aCCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGc                                                                   >  1:202910/1‑148 (MQ=255)
                                                                               aCCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGc                                                                   >  3:188752/1‑148 (MQ=255)
                                                                                   gTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAgcg                                                              >  7:77257/1‑149 (MQ=255)
                                                                                   gTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAgcg                                                              >  8:268163/1‑149 (MQ=255)
                                                                                              gCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCg                                                   <  3:199858/149‑1 (MQ=255)
                                                                                                 gATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGaac                                                <  8:75044/149‑1 (MQ=255)
                                                                                                   tGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGaacaa                                              <  4:93987/149‑1 (MQ=255)
                                                                                                     cGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGaacaagt                                            <  7:268163/149‑3 (MQ=255)
                                                                                                         aagtgaaACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTcc                                        >  3:128002/1‑149 (MQ=255)
                                                                                                         aagtgaaACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTcc                                        >  3:76590/1‑149 (MQ=255)
                                                                                                         aagtgaaACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTc                                         <  8:77257/148‑1 (MQ=255)
                                                                                                           gtgaaACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCtg                                      >  3:395372/1‑149 (MQ=255)
                                                                                                           gtgaaACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCtg                                      >  6:262175/1‑149 (MQ=255)
                                                                                                               aaCAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGaa                                                 <  7:298743/134‑1 (MQ=255)
                                                                                                               aaCAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGaa                                                 >  8:298743/1‑134 (MQ=255)
                                                                                                                        gAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCa                                                                 >  1:398674/1‑109 (MQ=255)
                                                                                                                        gAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCa                                                                 <  2:398674/109‑1 (MQ=255)
                                                                                                                          ggCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCTGTgc                                   >  8:4036/1‑137 (MQ=255)
                                                                                                                          ggCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCTGTgc                                   <  7:4036/137‑1 (MQ=255)
                                                                                                                           gCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCTGTGCGCATTTcacgcac                      <  3:268560/149‑1 (MQ=255)
                                                                                                                             cTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCTGTGCGCATTTCACGCACgg                    >  3:396784/1‑149 (MQ=255)
                                                                                                                                gATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGaac                                                >  3:179756/1‑118 (MQ=255)
                                                                                                                                gATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGaac                                                <  4:179756/118‑1 (MQ=255)
                                                                                                                                     ggggCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCTGTGCGCATTTCACGCACGGCCTTCCCg            >  1:118622/1‑149 (MQ=255)
                                                                                                                                        gCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCTGTGCGCATTTCACGCACGGCCTTCCCGCCt         <  4:188752/149‑1 (MQ=255)
                                                                                                                                        gCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCTGTGCGCATTTCACGCACGGCCTTCCCGCCt         <  4:395372/149‑1 (MQ=255)
                                                                                                                                               aGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCTGTGCGCATTTCACGCACGGCCTTCCCGCCTGgcgcag  <  4:76590/149‑3 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAACAGGGCTGAGGCCTTGATCAGGGGCCGCGCAGCGGCCCCTTTCACGTTTGCTCAGGCGGGCAGCGCAAAAAATGTGCGCATCAACGAATCATCCTCGACACCCAGTCGCCCCGCCAGCGCCTGGCGATCGAACAACTGTCCTGTGCGCATTTCACGCACGGCCTTCCCGCCTGGCGCCT  >  NC_002947/5777260‑5777551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑