| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,807,986 | G→A | 5.3% | intergenic (+121/+34) | mrcA → / ← maeB | penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,807,986 | 0 | G | A | 5.3% | 289.5 / 3.3 | 96 | intergenic (+121/+34) | mrcA/maeB | penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B |
| Reads supporting (aligned to +/- strand): ref base G (48/41); new base A (3/2); total (52/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.78e-01 | |||||||||||
TGAAGCGACGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGTGA > NC_002947/5807838‑5808132 | tGAAGCGACGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATg < 2:137727/149‑1 (MQ=255) aCGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCg > 1:189818/1‑149 (MQ=255) cGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCgg < 2:382801/149‑1 (MQ=255) cGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCAAAAGTGCCCTGACCATCACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCgg < 7:13243/149‑1 (MQ=255) ccGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATTAAA‑AAGCCCCGGCTCATGCGAGCCgggg < 1:228799/149‑1 (MQ=255) gATGGCTCTGTTCTAACGCACGGCGCCTGACTCAGGGTTTGCGGGGGCGCGCGATCTGATACGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAACAAGCCCCGGCTCATGCGAGCCGGGGc < 1:6431/149‑1 (MQ=255) tGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGATACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCttt < 1:307683/149‑1 (MQ=255) tCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGTGCGATCGATCTTATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCACCATAAAA‑AATCCCCGGCTCATCCGAGCCCGGGCTATTTTa > 6:252983/1‑149 (MQ=255) tCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTa > 6:114419/1‑149 (MQ=255) tCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTa > 7:57513/1‑149 (MQ=255) gTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGt > 4:257856/1‑149 (MQ=255) ttCTAAGTCAGGGCGCCTGACTCAGGTTTTGCGTGTGCGCTCGATCTGTTAGGAGCAACAAGTGCCTTGACCAACACCTTGCAGCCATGACTCATTCCCAAGCCCCATAAAA‑AAGACCCGTATCATGCTAGCCGTGGCTTTTTTa < 4:82896/145‑1 (MQ=255) ttCTAAGGCAGGGCGCCTGACTCAGTTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCtttttt < 8:29966/144‑1 (MQ=255) ttCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTc > 6:142198/1‑149 (MQ=255) ttCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGAGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCATGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTAGGTc > 6:289870/1‑149 (MQ=255) cTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGc > 1:267758/1‑149 (MQ=255) tAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCt > 8:112170/1‑149 (MQ=255) tAAGGCAGGGCGCCTGACTCAGGTTTCGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCt > 6:73834/1‑149 (MQ=255) gCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTtgct < 3:408392/149‑1 (MQ=255) aGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTtgctgc < 8:5699/149‑1 (MQ=255) aCTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGt < 5:28451/149‑1 (MQ=255) cTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGtt < 6:25909/149‑1 (MQ=255) aGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTg < 5:114419/147‑1 (MQ=255) gTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGaa > 6:307776/1‑147 (MQ=255) gTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGaa < 5:307776/147‑1 (MQ=255) ggggggGCCCGATCCGAAACGCGCCACAAGTGCCCCGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATc < 5:246080/147‑1 (MQ=255) ggCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCAc < 8:57513/149‑1 (MQ=255) gcTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCt > 1:56085/1‑149 (MQ=255) gcTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AACCCCCGGCTCATGCGAGCCGGGGCtttt < 3:159893/104‑1 (MQ=255) gcTAGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGACCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCtttt > 4:159893/1‑104 (MQ=255) cTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAg < 3:147225/125‑1 (MQ=255) cTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAg > 4:147225/1‑125 (MQ=255) cTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTg < 5:193674/149‑1 (MQ=255) tCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATACACGCTgg > 3:215254/1‑149 (MQ=255) aTCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCa > 7:279842/1‑149 (MQ=255) gATAGGCGCCACAAGTGCCCTGACCAACGCCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTtg > 1:231099/1‑107 (MQ=255) gATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTtg < 2:231099/107‑1 (MQ=255) cccTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATaa < 8:279842/148‑1 (MQ=255) cccTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATaa < 5:252983/148‑1 (MQ=255) cccTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATACACGCTGGTCAGCGGGTAATGCTTCGGATaa < 4:215254/148‑1 (MQ=255) ccTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCACAAGCGAGCCGGGGCTATTTTATGTCGCTTGGTGCAATCAGCCGTTGAACACTTCATCCACCGTGGTGAGCGGGTAATGGTTCGGATAAgg > 8:62803/1‑149 (MQ=255) ccTGACAAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTATCTTGCTGCAATCAGCCGTTGAAAACATCATCCACGCTGGTCAGAGGGTAATGCTTCGGATaacg > 4:209151/1‑147 (MQ=255) tGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTc < 6:203330/139‑1 (MQ=255) tGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTc > 5:203330/1‑139 (MQ=255) tGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAgg < 4:232654/147‑1 (MQ=255) tGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAgg > 3:232654/1‑147 (MQ=255) aCCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCa < 7:112170/147‑1 (MQ=255) ccAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAggg < 2:189818/149‑1 (MQ=255) cAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAggg > 7:36712/1‑148 (MQ=255) cAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAggg > 7:128551/1‑148 (MQ=255) aaCACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAggg > 2:2448/1‑147 (MQ=255) acacCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAg > 5:358600/1‑149 (MQ=255) acacCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAg > 4:7996/1‑149 (MQ=255) gCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGcc > 4:195310/1‑149 (MQ=255) gCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCAAGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGAGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGcc > 4:201454/1‑149 (MQ=255) cAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGcc < 5:142198/148‑1 (MQ=255) ccTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAAGACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGAtt > 7:55362/1‑149 (MQ=255) ccTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGAtt < 1:2448/149‑1 (MQ=255) tGACACATTCCCAAGCCCCATAAAA‑AAGCCGCGGCTCATTCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATcc < 6:416816/95‑1 (MQ=255) tGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGCGCCGGGGCTTTTTAATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATcc > 5:416816/1‑95 (MQ=255) gACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGa > 4:50653/1‑149 (MQ=255) acaTTCCCAAGCCCCATAAAA‑AAGCACCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAAACAGCCGTAGAACACTTCATCCTCGCTGGTAAGCGGGATATGCTTAGGATAAGGCAGGGTAGCACCGCCCGCTTAGATgg > 3:268230/1‑149 (MQ=255) aTTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATgg > 3:353169/1‑147 (MQ=255) ccAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCt > 7:334197/1‑149 (MQ=255) cAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCAc < 4:2413/86‑1 (MQ=255) cAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCAc > 3:2413/1‑86 (MQ=255) aGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTgg < 5:289870/149‑1 (MQ=255) ccATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGAt > 4:72165/1‑134 (MQ=255) ccATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGAt < 3:72165/134‑1 (MQ=255) gaTAAAA‑AAGCCCCGGGTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGTTTTCGATGGCAGCCTTGGCcaca < 7:62803/148‑1 (MQ=255) cATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATg < 2:353195/97‑1 (MQ=255) cATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATg > 1:353195/1‑97 (MQ=255) cATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCcaca < 3:120311/149‑1 (MQ=255) aTAAAA‑AAGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCtt > 6:52479/1‑141 (MQ=255) aTAAAA‑AAGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCtt < 5:52479/141‑1 (MQ=255) aTAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCca > 4:108385/1‑146 (MQ=255) aTAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAg > 2:199966/1‑149 (MQ=255) aTAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAg > 7:297966/1‑149 (MQ=255) aTAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAg > 4:326751/1‑149 (MQ=255) tAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAg > 2:280535/1‑148 (MQ=255) aaaa‑aaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAgg > 6:372017/1‑110 (MQ=255) aaaa‑aaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAgg < 5:372017/110‑1 (MQ=255) aaa‑aaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCAt > 5:143999/1‑149 (MQ=255) aa‑aaGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTg < 4:381785/78‑1 (MQ=21) aa‑aaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATc < 8:55362/149‑1 (MQ=255) a‑aaGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGGGGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCg > 7:381679/1‑149 (MQ=38) a‑aaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCg < 1:173198/149‑1 (MQ=255) aaGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCg > 2:203986/1‑148 (MQ=255) aGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCgg > 3:403185/1‑148 (MQ=255) gCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAacgacg < 2:267758/149‑1 (MQ=255) gCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAacgacg < 8:297966/149‑1 (MQ=255) cATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCgg > 8:141922/1‑85 (MQ=255) cATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCgg < 7:141922/85‑1 (MQ=255) cATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGt > 4:65657/1‑148 (MQ=255) aTGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTc > 8:372593/1‑82 (MQ=255) aTGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATTGCAGCCTTGGCCACAGCATCGGAAACGACGGt < 3:108385/147‑1 (MQ=255) aTGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGAGGGAAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATAGGAAACGACGGTGa > 3:114765/1‑149 (MQ=255) | TGAAGCGACGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACACCTTGCAGCCCTGACACATTCCCAAGCCCCATAAAA‑AAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGTGA > NC_002947/5807838‑5808132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |