| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,816,917 | C→T | 15.1% | intergenic (+103/+26) | PP_5092 → / ← pilT | NLP/P60 protein/twitching motility protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,816,917 | 0 | C | T | 15.1% | 77.6 / 5.7 | 33 | intergenic (+103/+26) | PP_5092/pilT | NLP/P60 protein/twitching motility protein |
| Reads supporting (aligned to +/- strand): ref base C (6/22); new base T (2/3); total (8/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01 | |||||||||||
TGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTCAGCATCCCCTCCGCCGCCCCGCCCTGCATCACTGAAGACAACTGCGCCAACCGC > NC_002947/5816774‑5817054 | tgctgcCGAAAGAGCAGCAGCGGAGTCTGGCCAAGCTTTAAGCTTATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCTTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCcg < 2:251790/149‑1 (MQ=255) tgcCTAACGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTTATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACGTGGGGGAGCGGGCGCGCCCGCGa < 6:9790/149‑1 (MQ=38) agcagcagcTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCtt < 8:386604/149‑1 (MQ=255) cTGAGTCTGGCCAAGCGTGAAGCTGATCGGCGCTATCCGGGACCGGCCCCTTCGCTGGCACGCCCGCGCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGGGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGta < 3:355144/149‑1 (MQ=38) gAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGtact < 5:342396/149‑1 (MQ=255) aGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTg < 7:189422/149‑1 (MQ=255) gCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGa < 3:90602/149‑1 (MQ=255) tCGGCTCTATCCTGTACCCGCCCCTTCGCGGGCACGCACGCTCCCTCCGGTTGCTCGTGGCTCTCGCGCGCAGCCCTCATACCTGTCGGAGCGGGCTCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCt < 6:382162/149‑1 (MQ=255) gcTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTc < 8:75090/149‑1 (MQ=255) ggCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGtac < 7:295296/106‑1 (MQ=255) ttCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCGGTGGGAGCGGGCGCGCCCGCAAAGgggccgggaccggagagcgccgatcagcgtaacgcttggccagactccgctgctgctctttcggc < 1:234702‑M2/149‑66 (MQ=255) gCACGCCGGCTGCCACAGGTAGCGGGTCGCTCTCGCGCGCAGCTCAGATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGt < 4:321668/149‑1 (MQ=255) cGCTCCCACAGGTCGCGCGTCGCTCTCGCGCGCAGCTCACATACGTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTCAGCATc < 3:169589/149‑1 (MQ=255) gCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAgggccggtaccggatagcgccgatcagcttaacgcttggccagactcagctgctgctctttcggcagcacccgcttggcc < 5:382994‑M2/149‑81 (MQ=255) cTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTCAGCATccc < 1:154017/149‑1 (MQ=255) tCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAgggcctttgcaggtaccggatagcgccgatcagcttaacgcttggccagactcagctgctgctctttcggcagcacccgctt < 4:28726‑M2/149‑83 (MQ=255) tCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAgggcctttgcaggtaccggatagcgccgatcagcttaacgcttggccagactcagctgctgctctttcggcagcacccgctt < 4:203662‑M2/149‑83 (MQ=255) cccACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGTGCCCGCGaag > 8:198474/1‑65 (MQ=255) cccACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGTGCCCGCGaag < 7:198474/66‑2 (MQ=255) ccACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAgggccggtaccggatagcgccgatcagcttaacgcttggccagactcagctgctgctctttcggcagcacccgcttggccacca < 3:93544‑M2/149‑85 (MQ=255) aGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAgggccggtaccggatagcgccgatcagcttaacgcttggccagactcagctgctgctctttcggcagcacccgcttggccaccacgta > 6:382994‑M2/1‑61 (MQ=255) gTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCTCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTCAGCATCCCCTCcgccgc < 8:366164/149‑1 (MQ=255) gTAGCGCGTCGCTCTCGCGCGCAGCTAACATACCTGTGGGAGCGGGCGTGCCCGCGAAGgggccggtaccggatagcgccgataagcttaacgcttggccagactcagctgctgctatttcggcagcacccgctttgcccccacctagt < 2:396875‑M2/149‑91 (MQ=255) gCTCTCGCGCTCAGCTCACATACCTGTGGGAGCGGGCGTGCCCGCGAAGgggccggtaccggatagcgccgatcagcttaacgcttggccagactcagctgctgctctttcggcagcacccgcttggccaccacgtagtgcttctgcca > 1:396875‑M2/1‑49 (MQ=255) tctcGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTCAGCAta > 8:337322/1‑125 (MQ=255) tcGCGCGCAGCGCACATACCTGTGGGAGCGGGCGCGCCCGCGAACGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTCAGCATCCCCTCCGccgccc < 6:325848/137‑1 (MQ=255) cgcAGCTCACATACCTGTGGGAGCGGGCGTGCCCGCGAAGgggccggtaccggatagcgccgatcagcttaacgcttggccagaatcagctgctgctctttcggcagcacccgcttggccaccacgtagtgcttctgccagtaaggctt < 8:326829‑M2/149‑110 (MQ=255) gcAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCtt < 7:105419/65‑1 (MQ=255) gcAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCtt > 8:105419/1‑65 (MQ=255) tCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTCAGCATCCCCGCCGCCGCCCCCCCCGGCATCACGGCAGACaa > 5:161417/1‑148 (MQ=255) acaTACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGcc > 7:314369/1‑93 (MQ=255) acaTACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGcc < 8:314369/93‑1 (MQ=255) tttcgCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTAAGAATCCCCTAAGCCGCCCCCCCCGGCGTCAATGAAGACAAAGGCGGCAACCga > 7:342486/5‑148 (MQ=255) | TGCTGCCGAAAGAGCAGCAGCTGAGTCTGGCCAAGCGTTAAGCTGATCGGCGCTATCCGGTACCGGCCCCTTCGCGGGCACGCCCGCTCCCACAGGTAGCGCGTCGCTCTCGCGCGCAGCTCACATACCTGTGGGAGCGGGCGCGCCCGCGAAAGGGCCTTTGCAGGTACTACAAGCCTTTGATCAACCCTCGCTCCCTCAGCCTCCGCAACGCCCCCTCCATCGTCAGCATCCCCTCCGCCGCCCCGCCCTGCATCACTGAAGACAACTGCGCCAACCGC > NC_002947/5816774‑5817054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |