| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,897,773 | G→A | 8.6% | intergenic (‑173/+61) | PP_5172 ← / ← PP_5173 | hypothetical protein/RND family transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,897,773 | 0 | G | A | 8.6% | 162.5 / 4.7 | 58 | intergenic (‑173/+61) | PP_5172/PP_5173 | hypothetical protein/RND family transporter |
| Reads supporting (aligned to +/- strand): ref base G (23/30); new base A (2/3); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01 | |||||||||||
AGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGA > NC_002947/5897634‑5897919 | agGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATg > 4:285837/1‑149 (MQ=255)agGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATg > 6:163688/1‑149 (MQ=255)agGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTATATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCAGCGAATACGGTGGTCGATg > 5:407919/1‑149 (MQ=255) aaGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATc < 8:168273/149‑1 (MQ=255) tGCGCCTGGCTTGAGGGCACTTTTCGTGAGTCGGGATTATTCTTAAAAATACTGTGTAAGAATTTTTATATAACCATGTATGACCACACGCCTCTGGTAGGAGCGGGTTAACCCGCGATTACGGTGGTCGATGCACCATGGCATTcgc < 6:420650/148‑1 (MQ=255) gcgcATGGATTGAGGGCAATAGTAGTGAGGCGTGATTATTCTTAAAAATACTCTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTcgcg < 2:239766/148‑1 (MQ=255) cgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCgg < 5:314377/148‑1 (MQ=255) cgcCTGGATTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCgg < 6:150067/148‑1 (MQ=255) gAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCg < 5:163688/147‑1 (MQ=255) ggCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTcgctg < 3:124980/135‑3 (MQ=25) gaaaTAGAAGTTAGGCGTGATTATTCTTAAAAATAGTTTTTAATAATTTTTATATAACCATTTAAGAGACAACGCGTCTGGTTGGAGCGGGTTTACCCGGGAATACGGGGGTCGATGCACCATGGCATTCGCGGGTAAACCCGCTCCTa < 6:407919/147‑1 (MQ=37) tagtagTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAg > 2:88892/1‑149 (MQ=255) tGAGGGGGTATTATTATTAAAAATAATGTTTAAGAATTTTTATATAACAATTTAAGAGAACACGCGTGTGGTAGGAGGGGGTTTACCGGCGAATACGGTGGTCGATGCACCATCGAATTCGCGGGTAAACCCGCTCCTACCAGGGGcc < 2:328303/148‑1 (MQ=37) gAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCACGGGCCa > 6:146583/1‑148 (MQ=255) gggATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCTGGTTTACCAGCGAAAACGGTGGTCGATGCAACATCTCAATCGCAGGTAAAACAGCTCCTACCAGGGGCCATATggg > 8:129959/1‑149 (MQ=255) tttCTTAAAAATACTTTTTAATATTTTTTATATAACAATTAAAGACAACACGCCTGTGTTAGTAGGGGTTTTACCCGCTAATAGGGTGGTGGATGCACCATCGCATTCGGGGTAAAACCCGCTCCTACCAGGGGCCATATGGGCCTTc < 2:169111/147‑1 (MQ=255) tCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAg < 1:88892/148‑1 (MQ=255) tCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAAAAGCGAATAAGGTGGTGGATGCACCATAGCATTAGCGGGTAAACCCGCTCATAACAGGGGCCATATGGGCCTTCAgg > 7:141642/1‑149 (MQ=255) ttAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTcgc < 5:147303/106‑1 (MQ=255) ttAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTcgc > 6:147303/1‑106 (MQ=255) ttAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCggg > 1:140124/1‑109 (MQ=255) ttAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCggg < 2:140124/109‑1 (MQ=255) ttAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAAGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAACCCGCTCCTAc > 5:158281/1‑124 (MQ=17) ttAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAAGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAACCCGCTCCTAc < 6:158281/124‑1 (MQ=17) aaaaaTACTGTTAAAGAATTTTTAAAAAACCATAAAAGACAACACGCCTCTGGTAGGAGCGGGTTAACCCGCCAATACGGTGGGCGATGCACCATCGCATTCGCGGGAAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGc < 5:146239/146‑1 (MQ=38) aaaaTACTGTTTAAGAATTTTTATATAACCATTAAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCGGGCgtgt < 3:153607/149‑1 (MQ=255) tACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCTTTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCt < 6:183203/149‑1 (MQ=255) tttAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGc > 5:12818/1‑149 (MQ=255) tttAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCAGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGTGACTTCATGAGTGTTTCTGCAGc > 6:289957/1‑149 (MQ=38) gAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTcgcg > 6:79119/1‑149 (MQ=255) tttttATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGAt < 7:129959/149‑1 (MQ=255) ttttATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGAt < 3:285837/148‑1 (MQ=255) tataACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTa < 2:287883/149‑1 (MQ=255) tAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTAACAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCAc < 5:289957/148‑1 (MQ=255) acacaaCACGCCTCTTGTAGGCGCGGTTTTACCCGCTAATACGGTGGTCTATGAACCATCGCATTCGCGTTTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTa < 7:259539/145‑1 (MQ=38) gataaCTCGCCTCTGGTAGGAGCGGGTTTACCCGCTAATACGGTGGTCGATGCACCATCTCATTCGCGGTTAAACCCGCTCCTACCAGGGGc < 2:303157/89‑1 (MQ=21) gagaACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGc > 1:303157/1‑92 (MQ=255) agaACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACa < 1:235253/149‑1 (MQ=255) ggTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTTAACCCGCTGCTACCACGGGGCATATGGGCCTTCAGGCGTGTTTCTGCGGCTGGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGaa > 5:201398/1‑149 (MQ=38) gCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACagcagc > 3:198469/1‑149 (MQ=255) gggTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTg > 4:191940/1‑111 (MQ=255) gggTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTg < 3:191940/111‑1 (MQ=255) ggTTTACCGGCGAATACGGTGGTCGATGCACCATCGCCTTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTAc < 5:332320/113‑1 (MQ=255) ggTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCATGCGTGGTTCTGCGGCTCGCGGCTCTTGtcc > 6:332320/1‑111 (MQ=255) gTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGGGTAGAACAGCAGCGt > 1:140231/1‑148 (MQ=39) ccGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACagcagc > 3:142419/1‑139 (MQ=25) ccGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACagcagc < 4:142419/139‑1 (MQ=25) ccGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAg < 5:379614/129‑1 (MQ=255) ccGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAg > 6:379614/1‑129 (MQ=255) ccGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACAGCAGCGTCAGCAAgg > 8:391942/1‑149 (MQ=255) cgcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACAGCAGCGTCAGCAAGGt < 5:79119/149‑1 (MQ=255) cgcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACAGCAGCGTCAGCAAGGt > 1:263191/1‑149 (MQ=255) gcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTa > 5:156384/1‑103 (MQ=255) gcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTa < 6:156384/103‑1 (MQ=255) gAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACAGCAGCGTCAGCAAGGTCGc < 7:391942/149‑1 (MQ=255) aTAGGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGTTGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACAGCAGCGTCAGCAAGGTCGCCa < 1:117571/149‑1 (MQ=255) tACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACAGCAGCGTCAGCAAGGTCGCCAc > 6:298759/1‑149 (MQ=255) cGGTGGTCTATCCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACAGCAGCGTCAGCAAGGTCGCCACga < 6:12818/149‑1 (MQ=255) | AGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCC‑GGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGA > NC_002947/5897634‑5897919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |