Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,930,415	G→A	5.9%	L153L (CTC→CTT)	PP_5198 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,930,415	0	G	A	5.9%	258.9 / 3.7	85	L153L (CTC→CTT)	PP_5198	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (45/35);  new base A (3/2);  total (48/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

CCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAATTCG  >  NC_002947/5930270‑5930562                                                                                                                                                   |                                                                                                                                                    ccGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGc                                                                                                                                                  <  6:40400/149‑1 (MQ=255)     cAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCa                                                                                                                                              >  4:406362/1‑149 (MQ=255)       gCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACg                                                                                                                                            >  4:94590/1‑149 (MQ=255)          ggCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGtat                                                                                                                                         <  5:356664/149‑1 (MQ=255)          ggCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGta                                                                                                                                          >  8:244061/1‑148 (MQ=255)             caccaTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAgg                                                                                                                                      >  1:242961/1‑149 (MQ=255)                caTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTCAAGCCGCaa                                                                                                                                             >  2:89317/1‑138 (MQ=255)                caTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTCAAGCCGCaa                                                                                                                                             <  1:89317/139‑2 (MQ=255)                 aTCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGttt                                                                                                                                  <  3:406362/149‑1 (MQ=255)                   cGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTa                                                                                                                                >  5:262124/1‑149 (MQ=255)                     aGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATc                                                                                                                              <  7:244061/149‑1 (MQ=255)                           aaaaTTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCcg                                                                                                                        >  2:376593/1‑149 (MQ=255)                           aaaaTTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAAACAACACATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTCCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCcg                                                                                                                        >  3:343113/1‑149 (MQ=255)                            aaaTTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCTCCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCcgc                                                                                                                       <  3:230156/149‑1 (MQ=255)                            aaaTTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCcgc                                                                                                                       <  7:37955/149‑1 (MQ=255)                                      gCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACt                                                                                                             >  2:388922/1‑149 (MQ=255)                                       ccACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGccac                                                                                                                  <  3:45756/143‑1 (MQ=255)                                       ccACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGccac                                                                                                                  >  4:45756/1‑143 (MQ=255)                                       ccACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGccac                                                                                                                  >  4:51074/1‑143 (MQ=255)                                       ccACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGccac                                                                                                                  <  3:51074/143‑1 (MQ=255)                                                 gcAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTCAAGCCGCaagc                                                                                                                                           <  1:132319/108‑4 (MQ=255)                                                 gcAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTCAAGCCGCaagc                                                                                                                                           >  2:132319/1‑105 (MQ=255)                                                     cGCCAAGGCCAGGGCGCTGCCCACCCTACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATcgc                                                                                              >  3:50334/1‑149 (MQ=255)                                                      gCCAAGGCCAGGGCGCTGCCCCCCATACAGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATcgcg                                                                                             >  2:19857/1‑149 (MQ=255)                                                        cAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGa                                                                                           >  5:166505/1‑149 (MQ=255)                                                         aatgCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGat                                                                                          <  4:343113/146‑1 (MQ=255)                                                               aGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGAc                                                                                    <  2:242961/149‑1 (MQ=255)                                                               aGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGAc                                                                                    <  6:161341/149‑1 (MQ=255)                                                                 ggCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCt                                                                                  <  2:205340/149‑1 (MQ=255)                                                                        cccACCATACCGGCACCGACAATCAACAGAGCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCTATCGCGGATATCGACCTGCaaa                                                                             <  7:374974/147‑1 (MQ=255)                                                                             caTACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCggca                                                                      <  6:166505/149‑1 (MQ=255)                                                                               tACCGGCACCGACAATCAACAGATCTGCGCGCATATCC‑ATGCCTTACGCCTGCCTCGATTGCCGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGCATATCGACCTGCAAACGCggcagg                                                                    >  7:6228/1‑149 (MQ=255)                                                                                aCCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCggcagg                                                                    >  4:144448/1‑148 (MQ=255)                                                                                  cGGCACCGACAATCAACAGATCTGCGCGCATTTCCGATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCggcaggca                                                                  >  1:61324/1‑149 (MQ=255)                                                                                  cGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCAt                                                                 >  3:342129/1‑149 (MQ=255)                                                                                  cGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCAt                                                                 >  6:113335/1‑149 (MQ=255)                                                                                  cGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTGTTATCGACCCGCGCCACCAACTCGCCGGCGCCCTCGCGGATATCGACCTGCAAACGCGGCAGGCAt                                                                 >  7:189593/1‑149 (MQ=255)                                                                                    gCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCAttt                                                               <  1:376593/149‑1 (MQ=255)                                                                                     cACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTc                                                              >  8:115258/1‑149 (MQ=255)                                                                                         gACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGc                                                          <  4:342129/149‑1 (MQ=255)                                                                                          aCAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGcc                                                         >  5:413728/1‑149 (MQ=255)                                                                                          aCAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGcc                                                         >  6:351581/1‑149 (MQ=255)                                                                                              tCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTg                                                     >  8:80929/1‑149 (MQ=255)                                                                                                aaCAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAgg                                                                                                                                      >  3:307428/1‑66 (MQ=255)                                                                                                aaCAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAgg                                                                                                                                      <  4:307428/66‑1 (MQ=255)                                                                                                 aCAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTggcg                                                  >  3:71347/1‑149 (MQ=255)                                                                                                     aTCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCct                                              <  6:262124/149‑1 (MQ=255)                                                                                                          cgcgcATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCctgc                                            <  6:363894/146‑1 (MQ=255)                                                                                                          cgcgcATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCct                                              <  7:80929/144‑1 (MQ=255)                                                                                                           gcgcATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGc                                        >  1:396756/1‑149 (MQ=255)                                                                                                           gcgcATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGc                                        <  6:413728/149‑1 (MQ=255)                                                                                                           gcgcATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGc                                        <  7:115258/149‑1 (MQ=255)                                                                                                           gcgcATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGc                                        <  1:388922/149‑1 (MQ=255)                                                                                                            cgcTTTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCg                                       <  8:6228/149‑1 (MQ=255)                                                                                                            cgcATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCg                                       <  4:71347/149‑1 (MQ=255)                                                                                                               aTTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGAt                                    >  8:362658/1‑149 (MQ=255)                                                                                                                 ttCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTg                                                           <  5:383046/124‑1 (MQ=255)                                                                                                                 ttCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTg                                                           >  6:383046/1‑124 (MQ=255)                                                                                                                    c‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTgg                                         >  2:315953/1‑139 (MQ=255)                                                                                                                    c‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTgg                                         <  1:315953/139‑1 (MQ=255)                                                                                                                    c‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGt                               >  6:189131/1‑149 (MQ=255)                                                                                                                    c‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGt                               <  5:113335/149‑1 (MQ=255)                                                                                                                      aTGCCTTACGCCTGCCTCGCTTGCGGCGTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTc                              <  5:351581/149‑1 (MQ=255)                                                                                                                        gCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTc                              >  7:330482/1‑147 (MQ=255)                                                                                                                         ccTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCcag                           >  2:107223/1‑149 (MQ=255)                                                                                                                          cTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCct                                              >  2:304945/1‑129 (MQ=255)                                                                                                                          cTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCct                                              <  1:304945/129‑1 (MQ=255)                                                                                                                          cTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCcag                           >  3:266862/1‑148 (MQ=255)                                                                                                                          cTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCcag                           >  7:6289/1‑148 (MQ=255)                                                                                                                           ttACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCcag                           >  6:154745/1‑147 (MQ=255)                                                                                                                           ttACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCcag                           >  6:1448/1‑147 (MQ=255)                                                                                                                           ttACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCcag                           >  3:47017/1‑147 (MQ=255)                                                                                                                                    cctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGGTGGCGGATTTCGTc                              >  5:242324/1‑135 (MQ=255)                                                                                                                                    cctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTc                              <  6:242324/135‑1 (MQ=255)                                                                                                                                    cctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGcatcat                <  5:154745/149‑1 (MQ=255)                                                                                                                                     ctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCatcatc               <  8:6289/149‑1 (MQ=255)                                                                                                                                     ctCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGAAGCGCatcatc               >  4:301313/1‑149 (MQ=255)                                                                                                                                       cGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGa             >  7:194563/1‑149 (MQ=255)                                                                                                                                         cTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACg           <  5:1448/149‑1 (MQ=255)                                                                                                                                          gtGCGGCTCAAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGt          >  3:232767/2‑149 (MQ=255)                                                                                                                                            gCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCt                                                                                  >  1:203234/1‑75 (MQ=255)                                                                                                                                            gCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCt                                                                                  <  2:203234/75‑1 (MQ=255)                                                                                                                                                cTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAAtt    >  5:93599/1‑149 (MQ=255)                                                                                                                                                 ttGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAATTc   >  4:86100/1‑149 (MQ=255)                                                                                                                                                 ttGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAATTc   >  8:142404/1‑149 (MQ=255)                                                                                                                                                   gAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCTGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAATTCg  <  1:107223/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CCGTCAGCGGGCCACCATCGAGCAACAAAATTTGCAGGCCACTGTGGCGCAACGCCAAGGCCAGGGCGCTGCCCACCATACCGGCACCGACAATCAACAGATCTGCGCGCATTTCC‑ATGCCTTACGCCTGCCTCGCTTGCGGCTTGAGCCGCACGTATAGGGTTTTATCGACCCGCGCCACCAACTCGCCGGCGCCATCGCGGATATCGACCTGCAAACGCGGCAGGCATTTCTTGCCGGTGGCGGCCTGCTGGCGGATTTCGTCCAGCAGCGCATCATCGACGTGGAATTCG  >  NC_002947/5930270‑5930562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑