Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,959,995	A→T	6.9%	noncoding (74/103 nt)	PP_mr63 ←	rnk_pseudo ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,959,995	0	A	T	6.9%	262.0 / 6.4	86	noncoding (74/103 nt)	PP_mr63	rnk_pseudo ncRNA
Reads supporting (aligned to +/- strand):  ref base A (38/42);  new base T (2/4);  total (40/46)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GCGTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTC  >  NC_002947/5959848‑5960141                                                                                                                                                    |                                                                                                                                                   gCGTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCaa                                                                                                                                                   <  1:129357/149‑1 (MQ=255)  cGTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTTTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTTCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAg                                                                                                                                                  <  3:148451/149‑1 (MQ=255)   gTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAgg                                                                                                                                                 <  4:203674/149‑1 (MQ=255)         tcgtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCgg                                                                                                                                             <  7:277329/147‑1 (MQ=255)         tcgtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCgg                                                                                                                                             <  7:335307/147‑1 (MQ=255)         tcgtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGC‑AAACCCCGCCCTTGGGCggg                                                                                                                                            <  5:383192/147‑1 (MQ=255)            tcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGttt                                                                                                                                        <  6:50987/149‑1 (MQ=255)            tcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGttt                                                                                                                                        <  5:393825/149‑1 (MQ=255)             cgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGCCTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGttt                                                                                                                                        >  3:100647/1‑148 (MQ=255)              gAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCGGGGtttt                                                                                                                                       <  6:207542/148‑2 (MQ=255)              gAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTga                                                                                                                                      <  6:114417/149‑1 (MQ=255)                ggCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCTTGGGCGGGGttttgct                                                                                                                                    <  1:227945/149‑5 (MQ=255)                 gCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGaa                                                                                                                                   <  5:159536/149‑1 (MQ=255)                     tCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAgg                                                                                                                                 <  3:300238/147‑1 (MQ=255)                     tCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGAAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCt                                                                                                                               >  7:51487/1‑149 (MQ=255)                           aGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCg                                                                                                                         >  5:60127/1‑149 (MQ=255)                           aGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCg                                                                                                                         >  5:251956/1‑149 (MQ=255)                               gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGc                                                                                                                          <  8:140386/144‑1 (MQ=255)                               gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCgagat                                                                                                                     >  7:140386/1‑145 (MQ=255)                               gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCAc                                                                                                                     >  2:240044/1‑149 (MQ=255)                               gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCAc                                                                                                                     <  8:189701/149‑1 (MQ=255)                               gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGC‑AAACCCCGCCCTTGGGCGGGGtt                                                                                                                                         <  2:153506/128‑1 (MQ=255)                               gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGC‑AAACCCCGCCCTTGGGCGGGGtt                                                                                                                                         >  1:153506/1‑128 (MQ=255)                                 tCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTg                                                                                                                            <  6:182940/140‑1 (MQ=255)                                 tCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTg                                                                                                                            >  5:182940/1‑140 (MQ=255)                                 tCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCAcc                                                                                                                    >  3:185965/1‑148 (MQ=255)                                    aGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACg                                                                                                                >  8:188016/1‑149 (MQ=255)                                              cGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                      <  3:257422/149‑1 (MQ=255)                                               gTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCTAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                      >  7:269147/1‑148 (MQ=255)                                                tCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGaca                                                                                                    <  7:188016/149‑1 (MQ=255)                                                  cAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGacac                                                                                                   >  2:366450/1‑148 (MQ=255)                                                         gTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAg                                                                                           >  3:361265/1‑149 (MQ=255)                                                            aGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGcc                                                                                         <  5:384445/148‑1 (MQ=255)                                                              gATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCggg                                                                                      <  5:223125/149‑1 (MQ=255)                                                                  aGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCaaa                                                                                  <  8:191258/149‑1 (MQ=255)                                                                    tgAAGGCTTGGTGCTCTTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATa                                                                                <  8:66825/148‑1 (MQ=255)                                                                    ggAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATTAAGCAAAACCCCGCCCAAGGGCGGGGGTGGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCaaa                                                                                  >  4:362803/1‑147 (MQ=255)                                                                      aaGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAAccc                                                                                                        >  6:190313/1‑123 (MQ=255)                                                                      aaGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAAccc                                                                                                        <  5:190313/123‑1 (MQ=255)                                                                      aaGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACt                                                                              >  2:384258/1‑149 (MQ=255)                                                                          cTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAg                                                                          >  7:181217/1‑149 (MQ=255)                                                                                 cTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                      <  1:230746/114‑1 (MQ=255)                                                                                 cTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                      >  2:230746/1‑114 (MQ=255)                                                                                  tCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                            >  8:158707/1‑75 (MQ=255)                                                                                   cATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGc                                                                                                               <  4:103297/103‑1 (MQ=255)                                                                                   cATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGc                                                                                                               >  3:103297/1‑103 (MQ=255)                                                                                    aTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGccc                                                                       <  6:21433/142‑1 (MQ=255)                                                                                    aTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGccc                                                                       >  5:21433/1‑142 (MQ=255)                                                                                           aGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGtgtg                                                         >  4:218276/1‑149 (MQ=255)                                                                                            gTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGc                                                        >  4:408387/1‑149 (MQ=255)                                                                                            gTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGc                                                        >  3:213352/1‑149 (MQ=255)                                                                                            gTCAGACTCATTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGc                                                        <  6:65596/149‑1 (MQ=255)                                                                                              cAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGcc                                                       <  8:51487/148‑1 (MQ=255)                                                                                                  cTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAATCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                  <  4:100647/149‑1 (MQ=255)                                                                                                  cTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                  <  6:251956/149‑1 (MQ=255)                                                                                                   tCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGaca                                                                                                    >  7:339100/1‑98 (MQ=255)                                                                                                   tCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGaca                                                                                                    <  8:339100/98‑1 (MQ=255)                                                                                                   tCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGcc                                                 >  4:338365/1‑149 (MQ=255)                                                                                                   tCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGCCAAATACTGAAGCCCCTGCGCTAAGTGTGCCTTAGcc                                                 >  6:363822/1‑149 (MQ=255)                                                                                                     cTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCt                                                <  3:338365/148‑1 (MQ=255)                                                                                                     cTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTg                                               >  7:391092/1‑149 (MQ=255)                                                                                                      tttGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAgtgt                                                          >  1:103883/1‑137 (MQ=255)                                                                                                      tttGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAgtgt                                                          <  2:103883/137‑1 (MQ=255)                                                                                                      tttGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAAACCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGc                                              >  2:408367/1‑149 (MQ=255)                                                                                                          aaCAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTATGAGAAGGCTTTGGCGTCACAGACTCACAACCCCACACTACCACAGCCGCGCAAATACTCAAGCCCCTGCGCTCAGTCTGCCTTAGCCTGCTcgc                                          >  7:388055/1‑149 (MQ=255)                                                                                                               gCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTCCCCACAGTGTGCCTTAGCCTGCTCgcgc                                        >  4:319324/1‑146 (MQ=255)                                                                                                                cAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGa                                    <  3:408387/149‑1 (MQ=255)                                                                                                                  gTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGAcc                                  >  4:161448/1‑149 (MQ=255)                                                                                                                   ttttCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGt                                                                                                                        <  7:325456/62‑1 (MQ=255)                                                                                                                   ttttCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGt                                                                                                                        >  8:325456/1‑62 (MQ=255)                                                                                                                     ttCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTg                                <  6:60127/148‑1 (MQ=255)                                                                                                                      tCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTgg                               <  1:366450/148‑1 (MQ=255)                                                                                                                        aaTGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGc                             <  3:161448/148‑1 (MQ=255)                                                                                                                             gCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgcg                       >  6:119799/1‑149 (MQ=255)                                                                                                                                 aTAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAg                   <  3:218276/149‑1 (MQ=255)                                                                                                                                  tAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGa                  <  8:181217/149‑1 (MQ=255)                                                                                                                                    aaGCAAAACCCCGCCCAACGGCAGCGTGTCAGAAGGCTTTGGCCTCACCGACGCAGCACCAGAAACTAACACAGCAGCGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTAAGCCAGCTCGCGCAAGACCTGGGCCTCGCggaacat                 >  2:103037/1‑143 (MQ=255)                                                                                                                                     aGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCt               <  4:185965/149‑1 (MQ=255)                                                                                                                                       cAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGc             >  6:267208/1‑149 (MQ=255)                                                                                                                                        aaaaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGcc            <  4:361265/149‑1 (MQ=255)                                                                                                                                         aaaCCCCGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGCCGCAGCCCCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCg           >  7:350809/1‑149 (MQ=255)                                                                                                                                         aaaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCg           <  4:213352/149‑1 (MQ=255)                                                                                                                                           aCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgc         <  3:319324/149‑1 (MQ=255)                                                                                                                                            ccccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgcg        >  2:79361/1‑149 (MQ=255)                                                                                                                                             cccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGgcgc       >  4:1088/1‑149 (MQ=255)                                                                                                                                              ccGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGcc      <  5:267208/149‑1 (MQ=255)                                                                                                                                                  ccAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTc  <  1:384258/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GCGTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTC  >  NC_002947/5959848‑5960141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑