Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 630,310 G→T 5.8% H461Q (CAC→CAA)  PP_0544 ← ethanolamine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947630,3100GT 5.8% 253.5 / 3.0 78H461Q (CAC→CAA) PP_0544ethanolamine transporter
Reads supporting (aligned to +/- strand):  ref base G (34/39);  new base T (2/3);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCGACCGTGTGTACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCAC  >  NC_002947/630170‑630455
                                                                                                                                            |                                                                                                                                                  
ggCCGACCGTGTGTACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTggcg                                                                                                                                            >  6:402712/1‑149 (MQ=255)
 gCCGACCGTGTGTACGAAACCTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTggcgg                                                                                                                                           <  7:301024/149‑1 (MQ=255)
  ccGACCGTGTGTACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTggcggc                                                                                                                                          >  5:74152/1‑149 (MQ=255)
  ccGACCGTGTGTACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTggcggc                                                                                                                                          >  5:295376/1‑149 (MQ=255)
  ccGACCGTGTGTACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTggcgac                                                                                                                                          >  1:28051/1‑147 (MQ=255)
   cGACCGTGTGTACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCt                                                                                                                                         >  1:279458/1‑149 (MQ=255)
     aCCGTGTGTACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGt                                                                                                                                       <  8:300045/149‑1 (MQ=255)
        gtgtgtACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGa                                                                                                                                    <  5:4845/149‑1 (MQ=255)
        gtgtgtACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGa                                                                                                                                    <  2:279458/149‑1 (MQ=255)
           tgtACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGTGGCTGTAGaaa                                                                                                                                  <  7:158023/148‑1 (MQ=255)
           tgtACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGaaa                                                                                                                                  <  8:110320/148‑1 (MQ=255)
           tgtACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAg                                                                                                                                 <  1:63186/149‑1 (MQ=255)
             tACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGc                                                                                                                                <  1:123757/148‑1 (MQ=255)
             tACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCa                                                                                                                               <  8:99047/149‑1 (MQ=255)
             tACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCa                                                                                                                               <  6:74152/149‑1 (MQ=255)
               cGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCaaa                                                                                                                             >  7:367971/1‑149 (MQ=255)
               cGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCa                                                                                                                               >  5:307158/1‑147 (MQ=255)
                  aaCTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTa                                                                                                                          <  3:173701/149‑1 (MQ=255)
                    cTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGACTGTAGAAAGCAAAGTAgg                                                                                                                        <  2:28051/149‑1 (MQ=255)
                     ttGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAgg                                                                                                                        >  5:102982/1‑148 (MQ=255)
                        ccATACAGAATCTCCAGAGCCGCGGGTTGCGCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCaa                                                                                                                     <  6:307158/148‑1 (MQ=255)
                        ccATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAAt                                                                                                                    >  7:210645/1‑149 (MQ=255)
                         cATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTTCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAAtt                                                                                                                   <  5:224522/149‑1 (MQ=255)
                           tACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCCGTGTGCCCGCCACCACTTGGTtgcgg                                                                                                                                           >  1:335192/1‑123 (MQ=255)
                           tACAGAATATCCAGAGCCGCTGGTTGCCCTTCGGCGGGTGGCGTCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCCACCACTTGGTtgcgg                                                                                                                                           <  2:335192/123‑1 (MQ=255)
                              aGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATAc                                                                                                              <  1:93370/149‑1 (MQ=255)
                              aGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATAc                                                                                                              >  3:228783/1‑149 (MQ=255)
                                aaTCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCg                                                                                                            <  6:414752/149‑1 (MQ=255)
                                aaTCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCg                                                                                                            <  4:228783/149‑1 (MQ=255)
                                  tctcCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑Ca                                                                                                         >  4:195999/1‑149 (MQ=255)
                                      cAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCaaa                                                                                                                             <  7:276041/126‑1 (MQ=255)
                                      cAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCaaa                                                                                                                             >  8:276041/1‑126 (MQ=255)
                                      cAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑Ca                                                                                                         <  5:240277/145‑1 (MQ=255)
                                       agagCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGAt                                                                                                    <  6:295376/149‑1 (MQ=255)
                                          gCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCaaa                                                                                                                             <  8:182457/122‑1 (MQ=255)
                                          gCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCaaa                                                                                                                             >  7:182457/1‑122 (MQ=255)
                                                      cccGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAAt                                                                                     <  2:95734/149‑1 (MQ=255)
                                                      cccGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAAt                                                                                     <  8:367971/149‑1 (MQ=255)
                                                        cgcgGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGa                                                                                   >  7:157181/1‑149 (MQ=255)
                                                        cgcgGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGa                                                                                   <  6:102982/149‑1 (MQ=255)
                                                         gcggcgGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGAc                                                                                  <  1:18449/149‑1 (MQ=255)
                                                               ggTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCcg                                                                            >  4:13885/1‑149 (MQ=255)
                                                                   gcggcgATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCgg                                                                         <  5:45854/148‑1 (MQ=255)
                                                                    cggcgATCAGTGCAGGGCCTGTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGTTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGt                                                                       <  3:303688/149‑1 (MQ=255)
                                                                        gATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAAc                                                                   >  8:147495/1‑149 (MQ=255)
                                                                          tCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCTAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCa                                                                 <  5:402712/149‑1 (MQ=255)
                                                                             gTGCAGGGCCTCTTCGGACTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAgg                                                               >  4:52772/1‑148 (MQ=255)
                                                                              tGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAg                                                                                                                                                    <  7:191522/63‑1 (MQ=255)
                                                                              tGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAg                                                                                                                                                    >  8:191522/1‑63 (MQ=255)
                                                                              tGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCAGCGGGTCAACCAGGaa                                                             >  5:204907/1‑149 (MQ=255)
                                                                                   ggCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCgg                                                        >  6:348863/1‑149 (MQ=255)
                                                                                     ccTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCTCCCAGGAATCCGGCg                                                      >  7:354860/1‑149 (MQ=255)
                                                                                     ccTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGc                                                       >  7:336949/1‑148 (MQ=255)
                                                                                      ctctTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGt                                                                       <  6:346873/131‑1 (MQ=255)
                                                                                       tctTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAg                                                                                            >  8:181547/1‑109 (MQ=255)
                                                                                       tctTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAg                                                                                            <  7:181547/109‑1 (MQ=255)
                                                                                       tctTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGAc                                                    >  6:273450/1‑149 (MQ=255)
                                                                                         ttCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCgg                                                                         <  4:56618/126‑1 (MQ=255)
                                                                                         ttCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCgg                                                                         >  3:56618/1‑126 (MQ=255)
                                                                                               cTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGa                                            <  8:210645/149‑1 (MQ=255)
                                                                                               cTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGa                                            <  4:384468/149‑1 (MQ=255)
                                                                                                  cTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGAt                                           <  8:336949/147‑1 (MQ=255)
                                                                                                              gAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAAt                                                                                                                <  8:220562/67‑1 (MQ=255)
                                                                                                              gAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAAt                                                                                                                >  7:220562/1‑67 (MQ=255)
                                                                                                                   ccTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAgcgc                         >  6:65841/1‑148 (MQ=255)
                                                                                                                    ctctTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAgcgc                         >  7:41947/1‑147 (MQ=255)
                                                                                                                     tctTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGTTATAGATCACTGCAGCGCCAATGCCAACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCCCGCCAGCACCAgcgc                         >  1:327912/1‑147 (MQ=255)
                                                                                                                       ttCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACgg                                               <  5:407279/122‑1 (MQ=255)
                                                                                                                       ttCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACgg                                               >  6:407279/1‑122 (MQ=255)
                                                                                                                         ccGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCg                  >  4:133759/1‑149 (MQ=255)
                                                                                                                           ggTGCGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGa                >  1:360340/1‑149 (MQ=255)
                                                                                                                             tacagccGCCACCACTTGGTTGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAgcgc                         >  6:198623/6‑138 (MQ=255)
                                                                                                                             tacagccGCCACCACTTGGTTGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAgcgc                         <  5:198623/133‑1 (MQ=255)
                                                                                                                               tgCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACTCCAGCACCAGCGCCAGGCCGGAtgtg            <  1:13132/149‑1 (MQ=255)
                                                                                                                               tgCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGAtgtg            <  8:157181/149‑1 (MQ=255)
                                                                                                                                 cactcAACCAATTGGTGTGGTCTGTATAAAGCAAAGTAGGCATTTAATACCG‑CATAGCTCTCTGCAGCGCCAATGTCTCCTCGCGGGTCAACCAGGAATCCGGCGAGCACGGCGTTGCACGCCAGCACCAGCGCCAGGc                    <  8:386524/135‑1 (MQ=255)
                                                                                                                                  ccGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAgcgc                         <  5:184611/133‑1 (MQ=255)
                                                                                                                                  ccGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAgcgc                         >  6:184611/1‑133 (MQ=255)
                                                                                                                                  ccGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGAtgtg            >  3:110161/1‑146 (MQ=255)
                                                                                                                                         cAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCAc  >  2:39986/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GGCCGACCGTGTGTACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCG‑CATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCAC  >  NC_002947/630170‑630455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑