Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,017,238 T→G 5.8% K32N (AAA→AAC)  PP_5267 ← cytochrome c5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,017,2380TG 5.8% 261.3 / 4.9 82K32N (AAA→AAC) PP_5267cytochrome c5
Reads supporting (aligned to +/- strand):  ref base T (37/40);  new base G (3/2);  total (40/42)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

GAGCAAGCCGCTGCCATGGCAGGCGTTGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGCCACGGATCGGCGA  >  NC_002947/6017091‑6017382
                                                                                                                                                   |                                                                                                                                                
gAGCAAGCCGCTGCCATGGCAGGCGTTGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGtt                                                                                                                                                 <  2:397179/149‑1 (MQ=255)
 aGCAAGCCGCTTCCATGGCAGGCGTTGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGttt                                                                                                                                                <  7:322175/149‑1 (MQ=255)
 aGCAAGCCGATGCCATGGCAGGCGTTGCAGTGGTGGGCGATTATTTCATCGGGCGTTTTGGCT‑CCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTg                                                                                                                                               <  8:308969/149‑1 (MQ=255)
  gCAAGCCGCTGCCATGGCAGGCGTTGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTg                                                                                                                                               <  5:80557/149‑1 (MQ=255)
    aaGCCGCTGCCATGGCAGGCGTTGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGc                                                                                                                                             <  7:179972/149‑1 (MQ=255)
         gCTGCCATGGCAGGCGTTGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATgt                                                                                                                                        >  7:38849/1‑149 (MQ=255)
          cTGCCATGGCAGGCGTTGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATgtc                                                                                                                                       <  7:300459/149‑1 (MQ=255)
              cATGGCAGGCGTTGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCa                                                                                                                                   <  1:103377/149‑1 (MQ=255)
                   cAGGCGTTGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGt                                                                                                                              >  4:30201/1‑149 (MQ=255)
                      ggcttGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGc                                                                                                                           <  2:181402/146‑1 (MQ=255)
                          tGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCg                                                                                                                       >  7:114484/1‑149 (MQ=255)
                            cAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATgtcgtc                                                                                                                                    >  3:358452/1‑134 (MQ=255)
                            cAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATgtcgtc                                                                                                                                    <  4:358452/134‑1 (MQ=255)
                             aGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGtt                                                                                                                    <  2:148286/149‑1 (MQ=255)
                                 cTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTgcgc                                                                                                                <  2:182486/149‑1 (MQ=255)
                                 cTTGGCGATGATTTCATCCGGCGTTTTGGCTACCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTgcgc                                                                                                                <  3:30201/149‑1 (MQ=255)
                                           atTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCa                                                                                                      <  4:261574/149‑1 (MQ=255)
                                            tttCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCAGTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGttt                                                                                                                                                >  1:289888/1‑106 (MQ=255)
                                            tttCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGttt                                                                                                                                                <  2:289888/106‑1 (MQ=255)
                                                    ggCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTACATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATCTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGa                                                                                              >  8:94139/1‑148 (MQ=255)
                                                            ggCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGgctg                                                                                     >  5:357590/1‑149 (MQ=255)
                                                            ggCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGgct                                                                                      <  8:38849/148‑1 (MQ=255)
                                                              cTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAAACGCCTGGAGCCGGTTGGcc                                                                                                                                            >  6:168205/1‑91 (MQ=38)
                                                              cTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAAACGCCTGGAGCCGGTTGGcc                                                                                                                                            <  5:168205/92‑2 (MQ=38)
                                                               tCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGgctg                                                                                     >  5:404118/1‑146 (MQ=255)
                                                               tCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGgctg                                                                                     <  6:404118/146‑1 (MQ=255)
                                                               tCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGctgctg                                                                                  >  2:145758/1‑149 (MQ=255)
                                                                 ccccGCCAGCCGCTGCAGGGGCCACTTCGATGCGTTTGCACTCGTGGCCCTGGACGCACAGCTGGCCTACCTGCTCCAGGCGGTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCTTTGCCCAGAGGGCGAATACGGctgctg                                                                                  <  1:413176/147‑1 (MQ=255)
                                                                  cccGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTa                                                                               >  3:183555/1‑149 (MQ=255)
                                                                  cccGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTa                                                                               <  2:80078/149‑1 (MQ=255)
                                                                   ccGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCAt                                                                                                                                  >  5:194832/1‑97 (MQ=255)
                                                                   ccGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCAt                                                                                                                                  <  6:194832/97‑1 (MQ=255)
                                                                      ccACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGc                                                                           <  4:183555/149‑1 (MQ=255)
                                                                        aCCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGc                                                                           >  7:383849/1‑147 (MQ=255)
                                                                          ccGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGc                                                                                                <  8:207910/124‑1 (MQ=255)
                                                                          ccGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGc                                                                                                >  7:207910/1‑124 (MQ=255)
                                                                          ccGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAg                                                                        >  1:182480/1‑148 (MQ=255)
                                                                          ccGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAg                                                                        >  4:52977/1‑148 (MQ=255)
                                                                          ccGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGc                                                                       <  3:52977/149‑1 (MQ=255)
                                                                             cTGCAGCGGCCACTTCCATGCTTTTCCACTCATGCCCCTGTACGCACACCTGGCCAACCGGCTCCTGGCGTTTGGAAATGTCGTAATTGGTCCCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCAtc                                                                    <  1:112272/149‑1 (MQ=255)
                                                                             cTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCAtc                                                                    <  1:145758/149‑1 (MQ=255)
                                                                                cAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCTCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCAtcttc                                                                 <  3:393084/149‑1 (MQ=255)
                                                                                  gCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGc                                                                           >  8:41106/1‑137 (MQ=255)
                                                                                  gCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGc                                                                           <  7:41106/137‑1 (MQ=255)
                                                                                    ggCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCttga                                                             <  2:182480/149‑1 (MQ=255)
                                                                                        aCTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACATGGCCAACCGGCTCCAGGCGTTTGGCATTGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCACAGATGGCGAATACGCCTGCTGGTACGGCCACCATCTTCttgattt                                                          >  8:155127/1‑148 (MQ=255)
                                                                                         cTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCttgatttga                                                        <  8:114484/149‑1 (MQ=255)
                                                                                              aTGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCttgatt                                                           >  7:34243/1‑141 (MQ=255)
                                                                                              aTGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCttgatt                                                           <  8:34243/141‑1 (MQ=255)
                                                                                              aTGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCAc                                                   >  5:255330/1‑149 (MQ=255)
                                                                                                gCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCAcgc                                                 >  6:298720/1‑149 (MQ=255)
                                                                                                    ttGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGaa                                              >  7:8311/1‑148 (MQ=255)
                                                                                                      gCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCttgatt                                                           <  4:291439/133‑1 (MQ=255)
                                                                                                      gCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCttgatt                                                           >  3:291439/1‑133 (MQ=255)
                                                                                                        aCTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACAccc                                         >  5:424252/1‑149 (MQ=255)
                                                                                                               gCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCAtggt                                  <  6:325710/149‑1 (MQ=255)
                                                                                                                cccTGGACGCACATCAGGCCAACGGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTGATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATGTTCTTGATTTGATTCACGCGAACACCCTCAtggtg                                 <  7:153105/149‑1 (MQ=255)
                                                                                                                 ccTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCAtggtgg                                <  8:383849/149‑1 (MQ=255)
                                                                                                                   tGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGc                               >  7:64828/1‑148 (MQ=255)
                                                                                                                         cacaCCTGGCCAAGAGGCTCCTGTCGTTTGGCATTGTCGTCATTGGTCGCAGCGGTTGCGGTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCAc                        <  6:424252/149‑1 (MQ=255)
                                                                                                                          acacCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCa                                                                                                      <  6:66296/70‑1 (MQ=255)
                                                                                                                          acacCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCa                                                                                                      >  5:66296/1‑70 (MQ=255)
                                                                                                                          acacCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACg                       >  2:72023/1‑149 (MQ=255)
                                                                                                                          acacCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACg                       >  6:312390/1‑149 (MQ=255)
                                                                                                                            acattgCCAAACGCCTGGAGCCGGTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCt                     >  1:340471/6‑149 (MQ=255)
                                                                                                                            acCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCt                     >  6:120856/1‑149 (MQ=255)
                                                                                                                            acCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCt                     >  6:184463/1‑149 (MQ=255)
                                                                                                                              cTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCAtg                                    >  3:23092/1‑132 (MQ=255)
                                                                                                                              cTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCAtg                                    <  4:23092/132‑1 (MQ=255)
                                                                                                                              cTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTcg                   >  7:345024/1‑149 (MQ=255)
                                                                                                                              attgCCAAACGCCTGGAGCCGGTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTcg                   >  3:79361/4‑149 (MQ=255)
                                                                                                                               tGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTcgc                  >  7:58422/1‑149 (MQ=255)
                                                                                                                                  ccAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGc               <  5:298720/149‑1 (MQ=255)
                                                                                                                                   cAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGcc              <  7:353473/149‑1 (MQ=255)
                                                                                                                                   cAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGc               >  1:368342/1‑148 (MQ=255)
                                                                                                                                   cAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGc               >  4:329092/1‑148 (MQ=255)
                                                                                                                                    aaCCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGCCa             <  6:357590/149‑1 (MQ=255)
                                                                                                                                    aaCCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCACGGCCa             >  5:85619/1‑149 (MQ=255)
                                                                                                                                      ccGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATAGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGCCACg           >  8:172610/1‑149 (MQ=255)
                                                                                                                                         gCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGc                      <  2:237106/135‑1 (MQ=255)
                                                                                                                                         gCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGc                      <  2:243318/135‑1 (MQ=255)
                                                                                                                                         gCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGCCACGGAt        >  3:367453/1‑149 (MQ=255)
                                                                                                                                           tgcAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGCCACGGATCg      >  2:264747/3‑149 (MQ=255)
                                                                                                                                             cAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGCCACGGATCgg     <  8:64828/148‑1 (MQ=255)
                                                                                                                                             cAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGCCACGGATCGGc    <  1:264747/149‑1 (MQ=255)
                                                                                                                                               ggCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATAGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGCCACGGATCGGCGa  <  7:172610/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GAGCAAGCCGCTGCCATGGCAGGCGTTGCAGTGCTTGGCGATGATTTCATCCGGCGTTTTGGCTCCCCCGCCACCCGCTGCAGCGGCCACTTCCATGCCTTTGCACTCCTGGCCCTGGACGCACACCTGGCCAACCGGCTCCAGGCGTTTGGCAATGTCGTCATTGGTCGCAGCGGTTGCGCTCATTGCCCAGAGGGCGAATACGGCTGCTGGTACGGCCAGCATCTTCTTGATTTGATTCACGCGAACACCCTCATGGTGGCTAATCACGCTCGCGGCCACGGATCGGCGA  >  NC_002947/6017091‑6017382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑