Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,041,127	T→A	9.2%	Q17H (CAA→CAT)	PP_5293 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,041,127	0	T	A	9.2%	220.4 / 10.4	76	Q17H (CAA→CAT)	PP_5293	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base A (4/3);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

TGATCTGAAAGTTCAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGG  >  NC_002947/6040979‑6041271                                                                                                                                                     |                                                                                                                                                 tGATCTGAAAGTTCAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAt                                                                                                                                                  >  7:319722/1‑149 (MQ=255)       gAAAGTTCAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCg                                                                                                                                             >  3:301943/1‑148 (MQ=255)        aaaGTTCAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGcc                                                                                                                                              <  8:384045/146‑1 (MQ=255)            ttCAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTc                                                                                                                                       >  6:104265/1‑149 (MQ=255)                gcgcCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCa                                                                                                                                   <  8:27795/149‑1 (MQ=255)                    ctctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCTGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAtt                                                                                                                                                 >  4:261711/1‑131 (MQ=255)                    ctctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAtt                                                                                                                                                 <  3:261711/131‑1 (MQ=255)                    ctctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAATGCCGAAATCCGGCAATgg                                                                                                                               <  6:313713/149‑1 (MQ=255)                     ccattGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTggg                                                                                                                              <  8:190024/146‑1 (MQ=255)                           cccTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCgggg                                                                                                                         <  4:51781/148‑1 (MQ=255)                                 gCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGa                                                                                                                  >  8:359/1‑149 (MQ=255)                                   gTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGa                                                                                                                  >  8:248658/1‑147 (MQ=255)                                      gATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGt                                                                                                             >  2:393943/1‑149 (MQ=255)                                        tAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTc                                                                                                           >  5:327667/1‑149 (MQ=255)                                           aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                        <  4:66480/149‑1 (MQ=255)                                           aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                        <  8:341746/149‑1 (MQ=255)                                           aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                        >  4:138811/1‑149 (MQ=255)                                            aGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAAt                                                                                                       <  2:187585/149‑1 (MQ=255)                                               gTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGc                                                                                                    >  8:120155/1‑149 (MQ=255)                                                     cTCGCGCTTGAGGAGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGc                                                                                              <  8:319722/149‑1 (MQ=255)                                                         cgcTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCg                                                                                          >  4:65237/1‑149 (MQ=255)                                                          gcTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGc                                                                                         <  6:327667/149‑1 (MQ=255)                                                               aGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcct                                                                                     <  3:138811/148‑1 (MQ=255)                                                                  tGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCtt                                                                                 <  4:301943/149‑1 (MQ=255)                                                                   gCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTg                                                                                <  7:120155/149‑1 (MQ=255)                                                                    ccATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCg                                                                                                     >  5:220770/1‑127 (MQ=255)                                                                    ccATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCg                                                                                                     <  6:220770/127‑1 (MQ=255)                                                                     cATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGc                                                                              >  2:256239/1‑149 (MQ=255)                                                                      aTAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCGGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCggg                                                                                                                          <  2:369011/104‑1 (MQ=255)                                                                      aTAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCGGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCggg                                                                                                                          >  1:369011/1‑104 (MQ=255)                                                                       tAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGGATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCg                                                                            >  7:297254/1‑149 (MQ=255)                                                                       tAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCg                                                                            >  5:251811/1‑149 (MQ=255)                                                                          cACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCTAATGCCGAAATCCGGCAATggg                                                                                                                              >  8:324681/1‑96 (MQ=255)                                                                          cACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCTAATGCCGAAATCCGGCAATggg                                                                                                                              <  7:324681/96‑1 (MQ=255)                                                                            ccAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCACTCATGCCCCGCACCTCCTTGGCCGGttt                                                                        >  2:391553/1‑148 (MQ=255)                                                                             cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGttt                                                                        >  3:368873/1‑147 (MQ=255)                                                                             cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtca                                                                      <  3:339966/149‑1 (MQ=255)                                                                             cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtca                                                                      <  7:359/149‑1 (MQ=255)                                                                             cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCAAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtca                                                                      <  5:104265/149‑1 (MQ=255)                                                                               gCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGACAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                    >  1:303705/1‑149 (MQ=255)                                                                                   gCCCAGCAGGTTCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGc                                                                <  6:251811/149‑1 (MQ=255)                                                                                     ccAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                    <  7:75515/143‑1 (MQ=255)                                                                                     ccAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                    >  8:75515/1‑143 (MQ=255)                                                                                       agcagGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGt                                                            >  6:212448/1‑149 (MQ=255)                                                                                          agGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGtgct                                                         >  6:72597/1‑149 (MQ=255)                                                                                            gTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                    >  8:125270/1‑136 (MQ=255)                                                                                            gTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                    <  7:125270/136‑1 (MQ=255)                                                                                             tGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGGATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcc                                                      >  7:384035/1‑149 (MQ=255)                                                                                              gCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcct                                                     >  5:53744/1‑149 (MQ=255)                                                                                                ccAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcctgc                                                   >  5:8522/1‑149 (MQ=255)                                                                                                 cAAGCGGCACTACCAGGACCAACAGCGCGGAGAGGGAGCAGAACATCGCCCATTGCAGGATTTCGGCATTGGGCGGGGTGATCGAAAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgct                                                  <  3:287294/149‑1 (MQ=255)                                                                                                  aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGtgctgc                                                       >  5:138186/1‑143 (MQ=255)                                                                                                  aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgctg                                                 >  5:25522/1‑149 (MQ=255)                                                                                                  aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgctg                                                 <  1:393943/149‑1 (MQ=255)                                                                                                  aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGGCAGCCAGtgctgc                                                       <  6:138186/143‑1 (MQ=255)                                                                                                          cTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCg                                          >  1:281486/1‑148 (MQ=255)                                                                                                             ccAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGaaa                                       <  6:53744/148‑1 (MQ=255)                                                                                                              cAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCa                                                                                                 >  7:215707/1‑89 (MQ=255)                                                                                                              cAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCa                                                                                                 <  8:215707/89‑1 (MQ=255)                                                                                                                 gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcag                                                                     <  1:151331/114‑1 (MQ=255)                                                                                                                 gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcag                                                                     >  2:151331/1‑114 (MQ=255)                                                                                                                 gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAtctc                                  >  7:214441/1‑149 (MQ=255)                                                                                                                     aaCAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTc                              <  4:368873/149‑1 (MQ=255)                                                                                                                        aGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATg                           >  4:74283/1‑149 (MQ=255)                                                                                                                            cgGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCt                       <  7:248658/149‑1 (MQ=255)                                                                                                                                 agGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTgc                  <  6:25522/149‑1 (MQ=255)                                                                                                                                  ggTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTgc                  <  2:303705/148‑1 (MQ=255)                                                                                                                                       cAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCaaa            <  8:214441/149‑1 (MQ=255)                                                                                                                                        aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAACTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAg           >  1:120182/1‑149 (MQ=255)                                                                                                                                        aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCACACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCt                    <  5:264937/140‑1 (MQ=255)                                                                                                                                        aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCACACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCt                    >  6:264937/1‑140 (MQ=255)                                                                                                                                             aTCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGc      <  6:8522/149‑1 (MQ=255)                                                                                                                                              tCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCa     <  5:72597/149‑1 (MQ=255)                                                                                                                                                gCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATg   >  3:132968/1‑149 (MQ=255)                                                                                                                                                gCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGGTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCAt    <  6:293554/148‑1 (MQ=255)                                                                                                                                                 cccAATGCCGAAATCCGGCAATGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATgg  >  2:178522/1‑149 (MQ=255)                                                                                                                                                 cccAATGCCGAAATCCGGCAATGGGCGGGGTGATAGACAGGTTCGAATCGCTCATGCCCCGCACATCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAAATCGTTCATGCAATTCTGCGCCAAAGCGAGCATg   >  8:133827/1‑148 (MQ=255)                                                                                                                                                  ccAATGCCGAAATCCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGtt                               >  4:373248/1‑119 (MQ=255)                                                                                                                                                  ccAATGCCGAAATCCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGtt                               <  3:373248/119‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 TGATCTGAAAGTTCAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGG  >  NC_002947/6040979‑6041271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑