Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,041,137	T→C	10.5%	E14G (GAA→GGA)	PP_5293 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,041,137	0	T	C	10.5%	200.9 / 12.5	76	E14G (GAA→GGA)	PP_5293	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/34);  new base C (5/3);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTCAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATC  >  NC_002947/6040990‑6041281                                                                                                                                                    |                                                                                                                                                 ttCAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTc                                                                                                                                                 >  6:104265/1‑149 (MQ=255)     gcgcCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCa                                                                                                                                             <  8:27795/149‑1 (MQ=255)         ctctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAATGCCGAAATCCGGCAATgg                                                                                                                                         <  6:313713/149‑1 (MQ=255)          ccattGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTggg                                                                                                                                        <  8:190024/146‑1 (MQ=255)                cccTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCgggg                                                                                                                                   <  4:51781/148‑1 (MQ=255)                      gCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGa                                                                                                                            >  8:359/1‑149 (MQ=255)                        gTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGa                                                                                                                            >  8:248658/1‑147 (MQ=255)                           gATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGt                                                                                                                       >  2:393943/1‑149 (MQ=255)                             tAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTc                                                                                                                     >  5:327667/1‑149 (MQ=255)                                aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                  <  4:66480/149‑1 (MQ=255)                                aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                  >  4:138811/1‑149 (MQ=255)                                aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                  <  8:341746/149‑1 (MQ=255)                                 aGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAAt                                                                                                                 <  2:187585/149‑1 (MQ=255)                                    gTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGc                                                                                                              >  8:120155/1‑149 (MQ=255)                                          cTCGCGCTTGAGGAGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGc                                                                                                        <  8:319722/149‑1 (MQ=255)                                              cgcTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCg                                                                                                    >  4:65237/1‑149 (MQ=255)                                               gcTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGc                                                                                                   <  6:327667/149‑1 (MQ=255)                                                    aGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcct                                                                                               <  3:138811/148‑1 (MQ=255)                                                       tGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCtt                                                                                           <  4:301943/149‑1 (MQ=255)                                                        gCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTg                                                                                          <  7:120155/149‑1 (MQ=255)                                                         ccATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCg                                                                                                               <  6:220770/127‑1 (MQ=255)                                                         ccATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCg                                                                                                               >  5:220770/1‑127 (MQ=255)                                                          cATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGc                                                                                        >  2:256239/1‑149 (MQ=255)                                                           aTAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCGGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCggg                                                                                                                                    >  1:369011/1‑104 (MQ=255)                                                           aTAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCGGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCggg                                                                                                                                    <  2:369011/104‑1 (MQ=255)                                                            tAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGGATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCg                                                                                      >  7:297254/1‑149 (MQ=255)                                                            tAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCg                                                                                      >  5:251811/1‑149 (MQ=255)                                                               cACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCTAATGCCGAAATCCGGCAATggg                                                                                                                                        >  8:324681/1‑96 (MQ=255)                                                               cACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCTAATGCCGAAATCCGGCAATggg                                                                                                                                        <  7:324681/96‑1 (MQ=255)                                                                 ccAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCACTCATGCCCCGCACCTCCTTGGCCGGttt                                                                                  >  2:391553/1‑148 (MQ=255)                                                                  cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGttt                                                                                  >  3:368873/1‑147 (MQ=255)                                                                  cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtca                                                                                <  3:339966/149‑1 (MQ=255)                                                                  cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtca                                                                                <  7:359/149‑1 (MQ=255)                                                                  cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCAAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtca                                                                                <  5:104265/149‑1 (MQ=255)                                                                    gCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGACAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                              >  1:303705/1‑149 (MQ=255)                                                                        gCCCAGCAGGTTCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGc                                                                          <  6:251811/149‑1 (MQ=255)                                                                          ccAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                              <  7:75515/143‑1 (MQ=255)                                                                          ccAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                              >  8:75515/1‑143 (MQ=255)                                                                            agcagGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGt                                                                      >  6:212448/1‑149 (MQ=255)                                                                               agGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGtgct                                                                   >  6:72597/1‑149 (MQ=255)                                                                                 gTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                              <  7:125270/136‑1 (MQ=255)                                                                                 gTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                              >  8:125270/1‑136 (MQ=255)                                                                                  tGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGGATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcc                                                                >  7:384035/1‑149 (MQ=255)                                                                                   gCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcct                                                               >  5:53744/1‑149 (MQ=255)                                                                                     ccAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcctgc                                                             >  5:8522/1‑149 (MQ=255)                                                                                      cAAGCGGCACTACCAGGACCAACAGCGCGGAGAGGGAGCAGAACATCGCCCATTGCAGGATTTCGGCATTGGGCGGGGTGATCGAAAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgct                                                            <  3:287294/149‑1 (MQ=255)                                                                                       aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGtgctgc                                                                 >  5:138186/1‑143 (MQ=255)                                                                                       aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgctg                                                           <  1:393943/149‑1 (MQ=255)                                                                                       aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgctg                                                           >  5:25522/1‑149 (MQ=255)                                                                                       aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGGCAGCCAGtgctgc                                                                 <  6:138186/143‑1 (MQ=255)                                                                                               cTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCg                                                    >  1:281486/1‑148 (MQ=255)                                                                                                  ccAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGaaa                                                 <  6:53744/148‑1 (MQ=255)                                                                                                   cAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCa                                                                                                           <  8:215707/89‑1 (MQ=255)                                                                                                   cAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCa                                                                                                           >  7:215707/1‑89 (MQ=255)                                                                                                      gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcag                                                                               >  2:151331/1‑114 (MQ=255)                                                                                                      gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcag                                                                               <  1:151331/114‑1 (MQ=255)                                                                                                      gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAtctc                                            >  7:214441/1‑149 (MQ=255)                                                                                                          aaCAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTc                                        <  4:368873/149‑1 (MQ=255)                                                                                                             aGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATg                                     >  4:74283/1‑149 (MQ=255)                                                                                                                 cgGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCt                                 <  7:248658/149‑1 (MQ=255)                                                                                                                      agGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTgc                            <  6:25522/149‑1 (MQ=255)                                                                                                                       ggTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTgc                            <  2:303705/148‑1 (MQ=255)                                                                                                                            cAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCaaa                      <  8:214441/149‑1 (MQ=255)                                                                                                                             aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAACTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAg                     >  1:120182/1‑149 (MQ=255)                                                                                                                             aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCACACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCt                              <  5:264937/140‑1 (MQ=255)                                                                                                                             aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCACACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCt                              >  6:264937/1‑140 (MQ=255)                                                                                                                                  aTCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGc                <  6:8522/149‑1 (MQ=255)                                                                                                                                   tCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCa               <  5:72597/149‑1 (MQ=255)                                                                                                                                    cgaacaatGCCGAAATCCGGAAATGGTCGGGGTGATCGACAGGTTCGAATCTCTCATGCCCATCAAATCCTTGGCCGTTTTCATTCAGCAAGTGCTGCCTTATGAAGCTCGAAAATATCGTTCATGCAATTCTGCGCCAAAGCGAGAAt              >  8:12168/8‑149 (MQ=255)                                                                                                                                     gCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATg             >  3:132968/1‑149 (MQ=255)                                                                                                                                     gCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGGTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCAt              <  6:293554/148‑1 (MQ=255)                                                                                                                                      cccAATGCCGAAATCCGGCAATGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATgg            >  2:178522/1‑149 (MQ=255)                                                                                                                                      cccAATGCCGAAATCCGGCAATGGGCGGGGTGATAGACAGGTTCGAATCGCTCATGCCCCGCACATCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAAATCGTTCATGCAATTCTGCGCCAAAGCGAGCATg             >  8:133827/1‑148 (MQ=255)                                                                                                                                       ccAATGCCGAAATCCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGtt                                         <  3:373248/119‑1 (MQ=255)                                                                                                                                       ccAATGCCGAAATCCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGtt                                         >  4:373248/1‑119 (MQ=255)                                                                                                                                                gATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATc  >  2:7062/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 TTCAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATC  >  NC_002947/6040990‑6041281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑