Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,041,143 T→A 7.8% N12I (AAT→ATT)  PP_5293 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,041,1430TA 7.8% 235.6 / 8.3 77N12I (AAT→ATT) PP_5293hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/35);  new base A (4/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

CTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGCT  >  NC_002947/6040998‑6041289
                                                                                                                                                 |                                                                                                                                                  
ctctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCAATGCCGAAATCCGGCAATgg                                                                                                                                                 <  6:313713/149‑1 (MQ=255)
 ccattGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTggg                                                                                                                                                <  8:190024/146‑1 (MQ=255)
       cccTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCgggg                                                                                                                                           <  4:51781/148‑1 (MQ=255)
             gCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGa                                                                                                                                    >  8:359/1‑149 (MQ=255)
               gTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGa                                                                                                                                    >  8:248658/1‑147 (MQ=255)
                  gATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGt                                                                                                                               >  2:393943/1‑149 (MQ=255)
                    tAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTc                                                                                                                             >  5:327667/1‑149 (MQ=255)
                       aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                          <  4:66480/149‑1 (MQ=255)
                       aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                          <  8:341746/149‑1 (MQ=255)
                       aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                          >  4:138811/1‑149 (MQ=255)
                        aGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAAt                                                                                                                         <  2:187585/149‑1 (MQ=255)
                           gTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGc                                                                                                                      >  8:120155/1‑149 (MQ=255)
                                 cTCGCGCTTGAGGAGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGc                                                                                                                <  8:319722/149‑1 (MQ=255)
                                     cgcTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCg                                                                                                            >  4:65237/1‑149 (MQ=255)
                                      gcTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGc                                                                                                           <  6:327667/149‑1 (MQ=255)
                                           aGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcct                                                                                                       <  3:138811/148‑1 (MQ=255)
                                              tGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCtt                                                                                                   <  4:301943/149‑1 (MQ=255)
                                               gCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTg                                                                                                  <  7:120155/149‑1 (MQ=255)
                                                ccATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCg                                                                                                                       >  5:220770/1‑127 (MQ=255)
                                                ccATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCg                                                                                                                       <  6:220770/127‑1 (MQ=255)
                                                 cATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGc                                                                                                >  2:256239/1‑149 (MQ=255)
                                                  aTAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCGGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCggg                                                                                                                                            >  1:369011/1‑104 (MQ=255)
                                                  aTAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCGGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCggg                                                                                                                                            <  2:369011/104‑1 (MQ=255)
                                                   tAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGGATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCg                                                                                              >  7:297254/1‑149 (MQ=255)
                                                   tAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCg                                                                                              >  5:251811/1‑149 (MQ=255)
                                                      cACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCTAATGCCGAAATCCGGCAATggg                                                                                                                                                <  7:324681/96‑1 (MQ=255)
                                                      cACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCTAATGCCGAAATCCGGCAATggg                                                                                                                                                >  8:324681/1‑96 (MQ=255)
                                                        ccAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCACTCATGCCCCGCACCTCCTTGGCCGGttt                                                                                          >  2:391553/1‑148 (MQ=255)
                                                         cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGttt                                                                                          >  3:368873/1‑147 (MQ=255)
                                                         cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtca                                                                                        <  7:359/149‑1 (MQ=255)
                                                         cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtca                                                                                        <  3:339966/149‑1 (MQ=255)
                                                         cAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCAAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtca                                                                                        <  5:104265/149‑1 (MQ=255)
                                                           gCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGACAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                                      >  1:303705/1‑149 (MQ=255)
                                                               gCCCAGCAGGTTCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGc                                                                                  <  6:251811/149‑1 (MQ=255)
                                                                 ccAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                                      <  7:75515/143‑1 (MQ=255)
                                                                 ccAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                                      >  8:75515/1‑143 (MQ=255)
                                                                   agcagGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGt                                                                              >  6:212448/1‑149 (MQ=255)
                                                                      agGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGtgct                                                                           >  6:72597/1‑149 (MQ=255)
                                                                        gTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                                      <  7:125270/136‑1 (MQ=255)
                                                                        gTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                                      >  8:125270/1‑136 (MQ=255)
                                                                         tGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGGATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcc                                                                        >  7:384035/1‑149 (MQ=255)
                                                                          gCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcct                                                                       >  5:53744/1‑149 (MQ=255)
                                                                            ccAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcctgc                                                                     >  5:8522/1‑149 (MQ=255)
                                                                             cAAGCGGCACTACCAGGACCAACAGCGCGGAGAGGGAGCAGAACATCGCCCATTGCAGGATTTCGGCATTGGGCGGGGTGATCGAAAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgct                                                                    <  3:287294/149‑1 (MQ=255)
                                                                              aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGtgctgc                                                                         >  5:138186/1‑143 (MQ=255)
                                                                              aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgctg                                                                   <  1:393943/149‑1 (MQ=255)
                                                                              aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgctg                                                                   >  5:25522/1‑149 (MQ=255)
                                                                              aaGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGGCAGCCAGtgctgc                                                                         <  6:138186/143‑1 (MQ=255)
                                                                                      cTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCg                                                            >  1:281486/1‑148 (MQ=255)
                                                                                         ccAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGaaa                                                         <  6:53744/148‑1 (MQ=255)
                                                                                          cAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCa                                                                                                                   <  8:215707/89‑1 (MQ=255)
                                                                                          cAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCa                                                                                                                   >  7:215707/1‑89 (MQ=255)
                                                                                             gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcag                                                                                       <  1:151331/114‑1 (MQ=255)
                                                                                             gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcag                                                                                       >  2:151331/1‑114 (MQ=255)
                                                                                             gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAtctc                                                    >  7:214441/1‑149 (MQ=255)
                                                                                                 aaCAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTc                                                <  4:368873/149‑1 (MQ=255)
                                                                                                    aGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATg                                             >  4:74283/1‑149 (MQ=255)
                                                                                                        cgGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCt                                         <  7:248658/149‑1 (MQ=255)
                                                                                                             agGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTgc                                    <  6:25522/149‑1 (MQ=255)
                                                                                                              ggTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTgc                                    <  2:303705/148‑1 (MQ=255)
                                                                                                                   cAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCaaa                              <  8:214441/149‑1 (MQ=255)
                                                                                                                    aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAACTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAg                             >  1:120182/1‑149 (MQ=255)
                                                                                                                    aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCACACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCt                                      <  5:264937/140‑1 (MQ=255)
                                                                                                                    aGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCACACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCt                                      >  6:264937/1‑140 (MQ=255)
                                                                                                                         aTCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGc                        <  6:8522/149‑1 (MQ=255)
                                                                                                                          tCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCa                       <  5:72597/149‑1 (MQ=255)
                                                                                                                           cgaacaatGCCGAAATCCGGAAATGGTCGGGGTGATCGACAGGTTCGAATCTCTCATGCCCATCAAATCCTTGGCCGTTTTCATTCAGCAAGTGCTGCCTTATGAAGCTCGAAAATATCGTTCATGCAATTCTGCGCCAAAGCGAGAAt                      >  8:12168/8‑149 (MQ=255)
                                                                                                                            gCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATg                     >  3:132968/1‑149 (MQ=255)
                                                                                                                            gCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGGTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCAt                      <  6:293554/148‑1 (MQ=255)
                                                                                                                             cccAATGCCGAAATCCGGCAATGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATgg                    >  2:178522/1‑149 (MQ=255)
                                                                                                                             cccAATGCCGAAATCCGGCAATGGGCGGGGTGATAGACAGGTTCGAATCGCTCATGCCCCGCACATCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAAATCGTTCATGCAATTCTGCGCCAAAGCGAGCATg                     >  8:133827/1‑148 (MQ=255)
                                                                                                                              ccAATGCCGAAATCCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGtt                                                 <  3:373248/119‑1 (MQ=255)
                                                                                                                              ccAATGCCGAAATCCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGtt                                                 >  4:373248/1‑119 (MQ=255)
                                                                                                                                       gATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATc          >  2:7062/1‑149 (MQ=255)
                                                                                                                                              gCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGc   >  6:155661/1‑149 (MQ=255)
                                                                                                                                              gCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTAAAATCTTCCGGc   >  5:246027/1‑149 (MQ=255)
                                                                                                                                               cATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGCt  <  1:7062/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCATTGCCGGATTTCGGCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGCT  >  NC_002947/6040998‑6041289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑