BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,108,695	C→G	5.9%	A315P (GCC→CCC) ‡	PP_5359 ←	CobW/P47K family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,108,695	0	C	G	5.9%	330.4 / 8.9	103	A315P (GCC→CCC) ‡	PP_5359	CobW/P47K family protein
Reads supporting (aligned to +/- strand): ref base C (45/50); new base G (3/3); total (49/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 103 total aligned reads displayed.

CACAGCGTGCAGATCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTCCCTTGG  >  NC_002947/6108549‑6108841

cacaGCGTGCAGATCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCtt                                                                                                                                                   <  1:79508/149‑1 (MQ=255)

        gCAGATCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAAcgcg                                                                                                                                          >  5:408969/1‑149 (MQ=255)

           gATCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggt                                                                                                                                       >  6:31544/1‑149 (MQ=255)

             tCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCggg                                                                                                                                        >  3:349933/1‑146 (MQ=255)

             tCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCggg                                                                                                                                        <  4:349933/146‑1 (MQ=255)

             tCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttg                                                                                                                                     <  5:250129/149‑1 (MQ=255)

             tCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTA‑GACACGCGggttg                                                                                                                                     >  6:406274/1‑149 (MQ=255)

               gAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttgcg                                                                                                                                   <  3:72903/149‑1 (MQ=255)

               gAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttgc                                                                                                                                    <  3:199590/148‑1 (MQ=255)

               gAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGCGTT‑GCAAGCCGggttgaa                                                                                                                                   <  2:80751/149‑3 (MQ=255)

                   tAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttgc                                                                                                                                    <  6:280561/144‑1 (MQ=255)

                   tAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttgc                                                                                                                                    >  5:280561/1‑144 (MQ=255)

                     gcgcCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGgttgcggttgcg                                                                                                                             >  6:193429/1‑149 (MQ=255)

                     gcgcCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGgttgcggttgcg                                                                                                                             <  5:21469/149‑1 (MQ=255)

                     gcgcCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACAGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttgcggttg                                                                                                                               <  1:292776/147‑1 (MQ=255)

                      ctcccTTCCCGTTCGAAATCAAGACCCCACCACTCGCCGACAACCTCATAGAGATGCAGCAATGGCGCCAGGAGCTTAACCGCTGGCGCAACTGATCAACTGCCGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGttgcggttgcgg                                                                                                                            <  7:224827/147‑1 (MQ=255)

                        cccTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCt                                                                                                                          <  1:411030/149‑1 (MQ=255)

                          cTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGa                                                                                                                        <  5:406274/149‑1 (MQ=255)

                          cTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGa                                                                                                                        <  1:226545/149‑1 (MQ=255)

                             cccGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTTCGCAACGCGGGTTGCGGTTGCGGCTGATg                                                                                                                      <  1:232865/149‑1 (MQ=255)

                                 ttCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGaaaa                                                                                                                 >  3:152667/1‑149 (MQ=255)

                                    gAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAAt                                                                                                                >  1:212030/1‑147 (MQ=255)

                                    gAAATCAAGACCCCACCAAGCGCCGACAACGTCATAGAGAAGCAGCAATGGCGCCAGCAGAAAAACACCTGCCGCAACTAATCAACAGACCCGACATTCATTCACCCCGGCTT‑GCAACGCCGGTTGCGGTTGCGGCTGAAGGAAAAt                                                                                                                >  8:320651/1‑147 (MQ=255)

                                     aaaTCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAg                                                                                                             >  5:52352/1‑149 (MQ=255)

                                     aaaTCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAg                                                                                                             <  5:31544/149‑1 (MQ=255)

                                          aaGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGAt                                                                                                                       <  4:207793/134‑1 (MQ=255)

                                          aaGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGAt                                                                                                                       >  3:207793/1‑134 (MQ=255)

                                           aGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGc                                                                                                                           >  6:92885/1‑129 (MQ=255)

                                           aGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGc                                                                                                                           <  5:92885/129‑1 (MQ=255)

                                            gACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAAGTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATa                                                                                                      >  1:130460/1‑149 (MQ=255)

                                            gACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATa                                                                                                      <  5:193429/149‑1 (MQ=255)

                                               cccACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑Gc                                                                                                                                                <  2:349755/104‑1 (MQ=255)

                                               cccACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACgg                                                                                                   <  7:171590/149‑1 (MQ=255)

                                                 caccacGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCt                                                                                                 <  1:260390/149‑1 (MQ=255)

                                                  accacGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTg                                                                                                >  7:136297/1‑149 (MQ=255)

                                                  accacGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTg                                                                                                >  1:65108/1‑149 (MQ=255)

                                                    cacGCGCAGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTc                                                                                              <  8:378408/149‑1 (MQ=255)

                                                            gACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTgcgc                                                                                       >  4:104764/1‑148 (MQ=255)

                                                               aaCGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGt                                                                                   <  4:152667/149‑1 (MQ=255)

                                                                    cATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACgg                                                                                                   <  7:198255/128‑1 (MQ=255)

                                                                    cATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACgg                                                                                                   >  8:198255/1‑128 (MQ=255)

                                                                       agagATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTc                                                                                  >  5:304938/1‑142 (MQ=255)

                                                                        gagaTGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCt                                                                          >  8:121773/1‑149 (MQ=255)

                                                                        gagaTGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCAGCGACTGGCGCAgggac                                                                          <  6:52352/149‑3 (MQ=255)

                                                                             gcagcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGcc                                                                       >  3:24740/1‑147 (MQ=255)

                                                                              cagcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCaa                                                                     <  8:109210/148‑1 (MQ=255)

                                                                              cagcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAg                                                                    <  7:121773/149‑1 (MQ=255)

                                                                               agcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGc                                                                   <  2:130460/149‑1 (MQ=255)

                                                                               agcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGCGTT‑GCAAGCCGggttgaa                                                                                                                                   <  5:152723/85‑3 (MQ=38)

                                                                               agcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGCGTT‑GCAAGCCGggttgaa                                                                                                                                   >  6:152723/1‑83 (MQ=38)

                                                                                gcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCg                                                                  <  2:65108/149‑1 (MQ=255)

                                                                                 caATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCgg                                                                 >  1:396369/1‑149 (MQ=255)

                                                                                  aaTGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCggg                                                                <  8:136297/149‑1 (MQ=255)

                                                                                   aTGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGc                                                               <  3:109224/149‑1 (MQ=255)

                                                                                     ggCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACgg                                                                                                   <  4:73846/111‑1 (MQ=255)

                                                                                     ggCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACgg                                                                                                   >  3:73846/1‑111 (MQ=255)

                                                                                      gcgcCAGGAGCATAACCGCTGGCGCAACTGAGCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCat                                                             <  7:320651/148‑1 (MQ=255)

                                                                                        gcCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATc                                                          >  5:373855/1‑149 (MQ=255)

                                                                                             gAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGcc                                                                       >  7:295108/1‑131 (MQ=255)

                                                                                             gAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGcc                                                                       <  8:295108/131‑1 (MQ=255)

                                                                                             gAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCAt                                                     >  5:245623/1‑149 (MQ=255)

                                                                                               gCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGc                                                               <  4:329726/137‑1 (MQ=255)

                                                                                               gCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGc                                                               >  3:329726/1‑137 (MQ=255)

                                                                                                 aTAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAg                                                 >  7:113828/1‑149 (MQ=255)

                                                                                                   aaCCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGTTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCa                                                  >  7:68457/1‑146 (MQ=255)

                                                                                                   aaCCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGTTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCa                                                  <  8:68457/146‑1 (MQ=255)

                                                                                                      cGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCAt                                            <  2:212030/149‑1 (MQ=255)

                                                                                                        cTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCAtt                                           >  7:88321/1‑148 (MQ=255)

                                                                                                                aCTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGacca                                  >  8:293683/1‑149 (MQ=255)

                                                                                                                       aaCTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCTGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCAATTGATTGACCACCCGccc                           >  2:100371/1‑149 (MQ=255)

                                                                                                                       aaCTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGccc                           >  3:148396/1‑149 (MQ=255)

                                                                                                                       aaCTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCGGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGcc                            >  8:150485/1‑148 (MQ=255)

                                                                                                                        aCTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCt                          <  6:348349/149‑1 (MQ=255)

                                                                                                                        aCTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCt                          >  5:348349/1‑149 (MQ=255)

                                                                                                                         cTGACGCGACATTCATTCAACCCGGCTT‑GCAACGAGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCACCATTCAGCCCATTGAATGACCACCAGCCCTc                         >  7:160608/1‑149 (MQ=255)

                                                                                                                          tGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGc                                                               <  5:81663/110‑1 (MQ=255)

                                                                                                                          tGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGc                                                               >  6:81663/1‑110 (MQ=255)

                                                                                                                              gcgACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGc                                                                        <  4:194887/97‑1 (MQ=255)

                                                                                                                              gcgACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGc                                                                        >  3:194887/1‑97 (MQ=255)

                                                                                                                                gACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCtg                  <  7:150485/149‑1 (MQ=255)

                                                                                                                                 aCATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCtgt                 <  7:293683/149‑1 (MQ=255)

                                                                                                                                   attcattcAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCtgtg                >  1:172021/1‑148 (MQ=255)

                                                                                                                                     tcattcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGAt                                                                                                       <  4:306792/59‑1 (MQ=255)

                                                                                                                                     tcattcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGAt                                                                                                       >  3:306792/1‑59 (MQ=255)

                                                                                                                                       attcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGTCCTCCATGCTGTGGATaa           <  6:245623/149‑1 (MQ=255)

                                                                                                                                       attcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATaa           <  4:148396/149‑1 (MQ=255)

                                                                                                                                       attcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATaa           <  6:373855/149‑1 (MQ=255)

                                                                                                                                       attaaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATa            >  4:380421/1‑148 (MQ=255)

                                                                                                                                        ttcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGtata            >  4:89888/1‑147 (MQ=255)

                                                                                                                                        ttcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATaa           >  7:195723/1‑148 (MQ=255)

                                                                                                                                        cgcaACCCGCGTT‑GCAAGCCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAAc          >  6:71829/3‑149 (MQ=255)

                                                                                                                                         tcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGaa                                        >  2:302156/1‑118 (MQ=255)

                                                                                                                                         tcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGaa                                        <  1:302156/118‑1 (MQ=255)

                                                                                                                                           aaCCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGacc                                   <  5:277336/121‑1 (MQ=255)

                                                                                                                                           aaCCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGacc                                   >  6:277336/1‑121 (MQ=255)

                                                                                                                                           aaCCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTc        >  3:7285/1‑148 (MQ=255)

                                                                                                                                           aaCCCGCGTT‑GCAAGCCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTcc       >  7:8195/1‑149 (MQ=255)

                                                                                                                                             cccGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGACTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCt                          <  2:189937/128‑1 (MQ=255)

                                                                                                                                             cccGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGACTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCt                          >  1:189937/1‑128 (MQ=255)

                                                                                                                                                ggCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTCCCTTgg  <  4:24740/149‑1 (MQ=255)

CACAGCGTGCAGATCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTCCCTTGG  >  NC_002947/6108549‑6108841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`