BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,108,702	C→G	5.7%	A312A (GCG→GCC) ‡	PP_5359 ←	CobW/P47K family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,108,702	0	C	G	5.7%	345.3 / 8.6	105	A312A (GCG→GCC) ‡	PP_5359	CobW/P47K family protein
Reads supporting (aligned to +/- strand): ref base C (49/50); new base G (3/3); total (52/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

Only 100 of 107 total aligned reads displayed.

GCAGATCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTCCCTTGGCCCGTCGCC  >  NC_002947/6108557‑6108850

   gATCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggt                                                                                                                                                >  6:31544/1‑149 (MQ=255)

     tCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCggg                                                                                                                                                 <  4:349933/146‑1 (MQ=255)

     tCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttg                                                                                                                                              <  5:250129/149‑1 (MQ=255)

     tCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTA‑GACACGCGggttg                                                                                                                                              >  6:406274/1‑149 (MQ=255)

       gAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttgcg                                                                                                                                            <  3:72903/149‑1 (MQ=255)

       gAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttgc                                                                                                                                             <  3:199590/148‑1 (MQ=255)

       gAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGCGTT‑GCAAGCCGggttgaa                                                                                                                                            <  2:80751/149‑3 (MQ=255)

           tAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttgc                                                                                                                                             >  5:280561/1‑144 (MQ=255)

           tAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttgc                                                                                                                                             <  6:280561/144‑1 (MQ=255)

             gcgcCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGgttgcggttgcg                                                                                                                                      <  5:21469/149‑1 (MQ=255)

             gcgcCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGgttgcggttgcg                                                                                                                                      >  6:193429/1‑149 (MQ=255)

             gcgcCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACAGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGggttgcggttg                                                                                                                                        <  1:292776/147‑1 (MQ=255)

              ctcccTTCCCGTTCGAAATCAAGACCCCACCACTCGCCGACAACCTCATAGAGATGCAGCAATGGCGCCAGGAGCTTAACCGCTGGCGCAACTGATCAACTGCCGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGttgcggttgcgg                                                                                                                                     <  7:224827/147‑1 (MQ=255)

                cccTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCt                                                                                                                                   <  1:411030/149‑1 (MQ=255)

                  cTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGa                                                                                                                                 <  5:406274/149‑1 (MQ=255)

                  cTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGa                                                                                                                                 <  1:226545/149‑1 (MQ=255)

                     cccGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTTCGCAACGCGGGTTGCGGTTGCGGCTGATg                                                                                                                               <  1:232865/149‑1 (MQ=255)

                         ttCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGaaaa                                                                                                                          >  3:152667/1‑149 (MQ=255)

                            gAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAAt                                                                                                                         >  1:212030/1‑147 (MQ=255)

                            gAAATCAAGACCCCACCAAGCGCCGACAACGTCATAGAGAAGCAGCAATGGCGCCAGCAGAAAAACACCTGCCGCAACTAATCAACAGACCCGACATTCATTCACCCCGGCTT‑GCAACGCCGGTTGCGGTTGCGGCTGAAGGAAAAt                                                                                                                         >  8:320651/1‑147 (MQ=255)

                             aaaTCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAg                                                                                                                      >  5:52352/1‑149 (MQ=255)

                             aaaTCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAg                                                                                                                      <  5:31544/149‑1 (MQ=255)

                                  aaGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGAt                                                                                                                                <  4:207793/134‑1 (MQ=255)

                                  aaGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGAt                                                                                                                                >  3:207793/1‑134 (MQ=255)

                                   aGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGc                                                                                                                                    <  5:92885/129‑1 (MQ=255)

                                   aGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGc                                                                                                                                    >  6:92885/1‑129 (MQ=255)

                                    gACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAAGTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATa                                                                                                               >  1:130460/1‑149 (MQ=255)

                                    gACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATa                                                                                                               <  5:193429/149‑1 (MQ=255)

                                       cccACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACgg                                                                                                            <  7:171590/149‑1 (MQ=255)

                                         caccacGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCt                                                                                                          <  1:260390/149‑1 (MQ=255)

                                          accacGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTg                                                                                                         >  7:136297/1‑149 (MQ=255)

                                          accacGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTg                                                                                                         >  1:65108/1‑149 (MQ=255)

                                            cacGCGCAGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTc                                                                                                       <  8:378408/149‑1 (MQ=255)

                                                    gACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTgcgc                                                                                                >  4:104764/1‑148 (MQ=255)

                                                       aaCGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGt                                                                                            <  4:152667/149‑1 (MQ=255)

                                                            cATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACgg                                                                                                            >  8:198255/1‑128 (MQ=255)

                                                            cATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACgg                                                                                                            <  7:198255/128‑1 (MQ=255)

                                                               agagATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTc                                                                                           <  6:304938/142‑1 (MQ=255)

                                                               agagATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTc                                                                                           >  5:304938/1‑142 (MQ=255)

                                                                gagaTGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCt                                                                                   >  8:121773/1‑149 (MQ=255)

                                                                     gcagcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGcc                                                                                >  3:24740/1‑147 (MQ=255)

                                                                      cagcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCaa                                                                              <  8:109210/148‑1 (MQ=255)

                                                                      cagcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAg                                                                             <  7:121773/149‑1 (MQ=255)

                                                                       agcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGc                                                                            <  2:130460/149‑1 (MQ=255)

                                                                       agcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGCGTT‑GCAAGCCGggttgaa                                                                                                                                            <  5:152723/85‑3 (MQ=38)

                                                                       agcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGCGTT‑GCAAGCCGggttgaa                                                                                                                                            >  6:152723/1‑83 (MQ=38)

                                                                        gcaATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCg                                                                           <  2:65108/149‑1 (MQ=255)

                                                                         caATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCgg                                                                          >  1:396369/1‑149 (MQ=255)

                                                                          aaTGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCggg                                                                         <  8:136297/149‑1 (MQ=255)

                                                                           aTGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGc                                                                        <  3:109224/149‑1 (MQ=255)

                                                                             ggCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACgg                                                                                                            <  4:73846/111‑1 (MQ=255)

                                                                             ggCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACgg                                                                                                            >  3:73846/1‑111 (MQ=255)

                                                                                gcCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATc                                                                   >  5:373855/1‑149 (MQ=255)

                                                                                     gAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGcc                                                                                >  7:295108/1‑131 (MQ=255)

                                                                                     gAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGcc                                                                                <  8:295108/131‑1 (MQ=255)

                                                                                     gAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCAt                                                              >  5:245623/1‑149 (MQ=255)

                                                                                       gCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGc                                                                        >  3:329726/1‑137 (MQ=255)

                                                                                       gCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGc                                                                        <  4:329726/137‑1 (MQ=255)

                                                                                         aTAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAg                                                          >  7:113828/1‑149 (MQ=255)

                                                                                           aaCCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGTTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCa                                                           >  7:68457/1‑146 (MQ=255)

                                                                                           aaCCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGTTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCa                                                           <  8:68457/146‑1 (MQ=255)

                                                                                              cGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCAt                                                     <  2:212030/149‑1 (MQ=255)

                                                                                                cTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCAtt                                                    >  7:88321/1‑148 (MQ=255)

                                                                                                        aCTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGacca                                           >  8:293683/1‑149 (MQ=255)

                                                                                                               aaCTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCTGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCAATTGATTGACCACCCGccc                                    >  2:100371/1‑149 (MQ=255)

                                                                                                               aaCTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGccc                                    >  3:148396/1‑149 (MQ=255)

                                                                                                               aaCTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCGGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGcc                                     >  8:150485/1‑148 (MQ=255)

                                                                                                                aCTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCt                                   <  6:348349/149‑1 (MQ=255)

                                                                                                                aCTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCt                                   >  5:348349/1‑149 (MQ=255)

                                                                                                                 cTGACGCGACATTCATTCAACCCGGCTT‑GCAACGAGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCACCATTCAGCCCATTGAATGACCACCAGCCCTc                                  >  7:160608/1‑149 (MQ=255)

                                                                                                                  tGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGc                                                                        >  6:81663/1‑110 (MQ=255)

                                                                                                                  tGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGc                                                                        <  5:81663/110‑1 (MQ=255)

                                                                                                                      gcgACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGc                                                                                 <  4:194887/97‑1 (MQ=255)

                                                                                                                      gcgACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGc                                                                                 >  3:194887/1‑97 (MQ=255)

                                                                                                                        gACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCtg                           <  7:150485/149‑1 (MQ=255)

                                                                                                                         aCATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCtgt                          <  7:293683/149‑1 (MQ=255)

                                                                                                                           attcattcAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCtgtg                         >  1:172021/1‑148 (MQ=255)

                                                                                                                             tcattcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGAt                                                                                                                >  3:306792/1‑59 (MQ=255)

                                                                                                                             tcattcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGAt                                                                                                                <  4:306792/59‑1 (MQ=255)

                                                                                                                              tattcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGTCTGATGGAAAATGAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCGCATTGAATGACCACCAGCCCTCCATGCTGTGGATa                     <  3:89888/148‑1 (MQ=255)

                                                                                                                               attcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGTCCTCCATGCTGTGGATaa                    <  6:245623/149‑1 (MQ=255)

                                                                                                                               attcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATaa                    <  6:373855/149‑1 (MQ=255)

                                                                                                                               attcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATaa                    <  4:148396/149‑1 (MQ=255)

                                                                                                                                ttcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGtata                     >  4:89888/1‑147 (MQ=255)

                                                                                                                                ttcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATaa                    >  7:195723/1‑148 (MQ=255)

                                                                                                                                cgcaACCCGCGTT‑GCAAGCCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAAc                   >  6:71829/3‑149 (MQ=255)

                                                                                                                                 tcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGaa                                                 <  1:302156/118‑1 (MQ=255)

                                                                                                                                 tcaACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGaa                                                 >  2:302156/1‑118 (MQ=255)

                                                                                                                                   aaCCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGacc                                            <  5:277336/121‑1 (MQ=255)

                                                                                                                                   aaCCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGacc                                            >  6:277336/1‑121 (MQ=255)

                                                                                                                                   aaCCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTc                 >  3:7285/1‑148 (MQ=255)

                                                                                                                                   aaCCCGCGTT‑GCAAGCCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTcc                >  7:8195/1‑149 (MQ=255)

                                                                                                                                     cccGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGACTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCt                                   >  1:189937/1‑128 (MQ=255)

                                                                                                                                        ggCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTCCCTTgg           <  4:24740/149‑1 (MQ=255)

                                                                                                                                              gCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTCCCTTGGCCCGt      >  8:240491/1‑149 (MQ=255)

                                                                                                                                              gCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTCCCTTGGCCCGt      >  4:205136/1‑149 (MQ=255)

                                                                                                                                                aaCGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGa                                                  >  5:357924/1‑103 (MQ=255)

                                                                                                                                                aaCGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGa                                                  <  6:357924/103‑1 (MQ=255)

                                                                                                                                                aaCGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTCCCTTGGCCCGTCg    >  1:175831/1‑149 (MQ=255)

                                                                                                                                                  cgcgGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTCCCTTGGCCCGTCGcc  <  2:333511/149‑1 (MQ=255)

GCAGATCGAACTAGCGCCCTACCCGTTCGAAATCAAGACCCCACCACGCGCCGACAACGTCATAGAGATGCAGCAATGGCGCCAGGAGCATAACCGCTGGCGCAACTGATCAACTGACGCGACATTCATTCAACCCGGCTT‑GCAACGCGGGTTGCGGTTGCGGCTGATGGAAAATCAGCTCGATACGGCTGTCCCTGCGCCAGTCGCTGGGCTGCCAAGCGGGCATATCCCCATTCAGCCCATTGAATGACCACCAGCCCTCCATGCTGTGGATAACTCCCTTGGCCCGTCGCC  >  NC_002947/6108557‑6108850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`