Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 657,690 C→T 6.9% R11W (CGG→TGG)  PP_0565 → Nudix hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947657,6900CT 6.9% 214.9 / 3.2 72R11W (CGG→TGG) PP_0565Nudix hydrolase
Reads supporting (aligned to +/- strand):  ref base C (28/39);  new base T (2/3);  total (30/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGCTCAACGGCAGTGGCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCAACAGTGCCGGTGAATTGTGTG  >  NC_002947/657542‑657837
                                                                                                                                                    |                                                                                                                                                   
tGCTCAACGGCAGTGGCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATc                                                                                                                                                     <  4:185247/149‑1 (MQ=255)
tGCTCAACGGCAGTGGCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATc                                                                                                                                                     >  3:60131/1‑149 (MQ=255)
 gCTCAACGGCAGTGGCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGTTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCg                                                                                                                                                    >  3:182113/1‑149 (MQ=255)
  cTCAACGGCAGTGGCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCgg                                                                                                                                                   <  3:177124/149‑1 (MQ=255)
   tCAACGGCAGTGGCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCggg                                                                                                                                                  >  6:186858/1‑149 (MQ=255)
          cAGTGGCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTc                                                                                                                                           <  1:114833/149‑1 (MQ=255)
           aGTGGCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTcc                                                                                                                                          <  7:33517/149‑1 (MQ=255)
           aGTGGCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTcc                                                                                                                                          <  7:58874/149‑1 (MQ=255)
               gCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGGTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGAc                                                                                                                                       <  4:182113/148‑1 (MQ=255)
                cTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACcg                                                                                                                                     >  6:724/1‑149 (MQ=255)
                cTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAAGCCGCCCGATGGGCGGCCTcggttgg                                                                                                                                     <  1:4381/149‑7 (MQ=255)
                 ttCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTcc                                                                                                                                          >  1:220073/1‑143 (MQ=255)
                 ttCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTcc                                                                                                                                          <  2:220073/143‑1 (MQ=255)
                        tgtgGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTgg                                                                                                                             <  5:330833/149‑1 (MQ=255)
                            ggCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCa                                                                                                                          >  6:282146/1‑148 (MQ=255)
                            ggCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCAc                                                                                                                         >  3:49466/1‑149 (MQ=255)
                              ccAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGATGCCGCCCATCGGGCGGCTTCCGACCCCGAGCTGGTCACCt                                                                                                                       <  4:128749/149‑1 (MQ=255)
                              ccAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCt                                                                                                                       >  3:79651/1‑149 (MQ=255)
                                aaCCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTgg                                                                                                                     <  8:166914/149‑1 (MQ=255)
                                aaCCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTgg                                                                                                                     >  3:331409/1‑149 (MQ=255)
                                 aCCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTggg                                                                                                                    <  4:331409/149‑1 (MQ=255)
                                  ccACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGTGGCAGCTGCTGTCGCAGGGTTTATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGt                                                                                                                   <  6:298204/149‑1 (MQ=255)
                                  ccACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGTCTTCCGACCGCGAGCTGGTCACCTGGGt                                                                                                                   <  5:724/149‑1 (MQ=255)
                                  caaCTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGTGGCTTCCGACCGCGAGCTGGTCACCTGGGt                                                                                                                   >  1:361120/3‑149 (MQ=255)
                                   cACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCAcc                                                                                                                        <  7:48705/143‑1 (MQ=255)
                                   cACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCAcc                                                                                                                        >  8:48705/1‑143 (MQ=255)
                                        gTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGAc                                                                                                               <  1:265848/147‑1 (MQ=255)
                                         ttGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGTGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGaa                                                                                                            <  2:361120/149‑1 (MQ=255)
                                               aTGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGAc                                                                                                      >  8:18679/1‑149 (MQ=255)
                                                tGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGAcc                                                                                                     <  4:380599/149‑1 (MQ=255)
                                                  gCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAg                                                                                                   <  1:62144/149‑1 (MQ=255)
                                                  gCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAg                                                                                                   <  8:195064/149‑1 (MQ=255)
                                                   ccAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAgg                                                                                                  <  4:60131/149‑1 (MQ=255)
                                                     aaCTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTa                                                                                                >  7:17134/1‑149 (MQ=255)
                                                       cTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACt                                                                                              <  8:272494/149‑1 (MQ=255)
                                                        tACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTg                                                                                             <  5:60442/149‑1 (MQ=255)
                                                          cATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTggg                                                                                           <  8:226980/149‑1 (MQ=255)
                                                           aTTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGt                                                                                                 <  6:347965/142‑1 (MQ=255)
                                                           aTTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGt                                                                                                 >  5:347965/1‑142 (MQ=255)
                                                           aTTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGgc                                                                                          <  5:186858/149‑1 (MQ=255)
                                                           aTTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGGGGACGAAACCGACCAGGTACTGGgc                                                                                          >  4:101389/1‑149 (MQ=255)
                                                            tttCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAAGCCGCCCGATGGGCGGCCTc                                                                                                                                           <  1:145005/99‑1 (MQ=255)
                                                            tttCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAAGCCGCCCGATGGGCGGCCTc                                                                                                                                           >  2:145005/1‑99 (MQ=255)
                                                              tCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGgcgc                                                                                        <  3:101389/148‑1 (MQ=255)
                                                                              cGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAg                                                                        <  4:49466/148‑1 (MQ=255)
                                                                                 ttATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTgc                                                                    <  8:17134/149‑1 (MQ=255)
                                                                                    tCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTgcgc                                                                  >  2:205949/1‑148 (MQ=255)
                                                                                        ccTGCGACAGCAGCTGACGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCg                                                             >  5:260702/2‑149 (MQ=255)
                                                                                            cGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTg                                                           <  7:18679/147‑1 (MQ=255)
                                                                                                aGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGa                                                     >  5:294476/1‑149 (MQ=255)
                                                                                                   agctgACGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCg                                                  <  6:260702/148‑1 (MQ=255)
                                                                                                     ctgTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCACATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACGAGGTACTGGTCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGt                                                <  6:375826/149‑1 (MQ=255)
                                                                                                        tCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGt                                             >  1:147752/1‑149 (MQ=255)
                                                                                                        tCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGt                                             >  3:215957/1‑149 (MQ=255)
                                                                                                                ttGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGtt                                     <  5:282146/149‑1 (MQ=255)
                                                                                                                ttGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCCGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGtt                                     >  1:154027/1‑149 (MQ=255)
                                                                                                                   atgatgGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGtgtg                                                           <  5:215910/124‑1 (MQ=255)
                                                                                                                   atgatgGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGtgtg                                                           >  6:215910/1‑124 (MQ=255)
                                                                                                                   atgatgGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCa                                   <  1:203872/148‑1 (MQ=255)
                                                                                                                            aTCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGtt                         >  6:394628/1‑149 (MQ=255)
                                                                                                                               aGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCaa                      <  1:205949/149‑1 (MQ=255)
                                                                                                                                gCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCaa                      >  5:14253/1‑148 (MQ=255)
                                                                                                                                gCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCaa                      >  1:20716/1‑148 (MQ=255)
                                                                                                                                gCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCaa                      >  8:226076/1‑148 (MQ=255)
                                                                                                                                    aaCCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCAACAGTg                 >  1:348077/1‑149 (MQ=255)
                                                                                                                                         aagccgccCGATGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCgg                                                 >  7:42926/3‑112 (MQ=255)
                                                                                                                                         aagccgccCGATGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCgg                                                 <  8:42926/110‑1 (MQ=255)
                                                                                                                                         aGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCAACAGTGCCGGt            <  7:16171/149‑1 (MQ=255)
                                                                                                                                         aGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCAACAGTGCCGGt            >  5:202285/1‑149 (MQ=255)
                                                                                                                                                ccATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCAACAGTGCCGGTGAATtgt     <  2:348077/149‑1 (MQ=255)
                                                                                                                                                 cATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCAACAGTGCCGGTGAATtgtg    >  7:141112/1‑149 (MQ=255)
                                                                                                                                                  aTCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCAACAGTGCCGGTGAATtgtgt   <  7:226076/149‑1 (MQ=255)
                                                                                                                                                  aTCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCAACAGTGCCGGTGAATtgtgt   <  5:394628/149‑1 (MQ=255)
                                                                                                                                                   tCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCAACAGTGCCGGTGAATtgtgtg  <  4:79651/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGCTCAACGGCAGTGGCTTCGGCATGTGGGCCAACCACTCGTTGGTCATGGCCAACTACATTTCCCGGGCCAACGCTGCGGTTATCGATCTGCGTCAGCTGCTGTCGCAGGGTTGATGATGGCTATCAGCCCAACCGAGGCCGCCCATCGGGCGGCTTCCGACCGCGAGCTGGTCACCTGGGTGGACGAAACCGACCAGGTACTGGGCGCCTTGCCCCGTGCCGAGCTGCGCGAGCGTGGCCTGATCGGTCGTTGCACGTTCATTCTGCTGTTCAACAGTGCCGGTGAATTGTGTG  >  NC_002947/657542‑657837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑