Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	675,587	A→T	6.1%	C38S (TGC→AGC)	PP_0577 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	675,587	0	A	T	6.1%	258.7 / 4.7	82	C38S (TGC→AGC)	PP_0577	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (40/36);  new base T (3/2);  total (44/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CTGGCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAG  >  NC_002947/675441‑675732                                                                                                                                                   |                                                                                                                                                  cTGGCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagc                                                                                                                                                 >  8:323867/1‑149 (MQ=255)    gCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagcc                                                                                                                                                <  4:91864/147‑1 (MQ=255)          gcgcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAgg                                                                                                                                         <  3:328539/148‑1 (MQ=255)          gcgcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAgg                                                                                                                                         <  3:350578/148‑1 (MQ=255)          gcgcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGGAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAgg                                                                                                                                         <  3:119544/148‑1 (MQ=255)           cgcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAggg                                                                                                                                        <  8:191797/148‑1 (MQ=255)            gcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCGACATACGGGTTGCTGCCCTGGCAGCCAGCCAgggga                                                                                                                                      >  3:302644/1‑147 (MQ=255)             ccagctccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCaa                                                                                                                                     >  8:329697/1‑149 (MQ=255)             ccagctccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCaa                                                                                                                                     >  8:335614/1‑149 (MQ=255)                  tccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATggg                                                                                                                                 >  7:288070/1‑148 (MQ=255)                      gctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGgcagca                                                                                                                            <  5:231812/149‑1 (MQ=255)                      gctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGgcagca                                                                                                                            <  7:323867/149‑1 (MQ=255)                       ctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGcagcag                                                                                                                           <  7:212073/149‑1 (MQ=255)                        tGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGccagccag                                                                                                                                          <  5:41513/133‑1 (MQ=255)                        tGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGccagccag                                                                                                                                          >  6:41513/1‑133 (MQ=255)                          cGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAggg                                                                                                                         >  3:87898/1‑148 (MQ=255)                                  cccATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGcac                                                                                                                 >  1:171038/1‑148 (MQ=255)                                  cccATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGcac                                                                                                                 >  3:219324/1‑148 (MQ=255)                                    cATAATAACGAGCGGGCGCATGGTAAGCACTGGGGTGGAAGGTGGTGGCCGCCTGGGGGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATTGGCAGCAGGGCCAGGCacaac                                                                                                              <  4:87898/149‑1 (MQ=255)                                     aTAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCacaa                                                                                                               >  7:278682/1‑147 (MQ=255)                                      tAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCacaa                                                                                                               >  5:150938/1‑146 (MQ=255)                                          cACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACg                                                                                                         <  8:288070/148‑1 (MQ=255)                                              aGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACg                                                                                                    <  2:171038/149‑1 (MQ=255)                                                    cgcAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGt                                                                                              <  4:219324/149‑1 (MQ=255)                                                    cgcAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGt                                                                                              <  5:361762/149‑1 (MQ=255)                                                     gcATGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGc                                                                                                  >  4:13235/1‑144 (MQ=255)                                                     gcAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGc                                                                                                  <  3:13235/144‑1 (MQ=255)                                                              gCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcg                                                                                    >  7:176681/1‑149 (MQ=255)                                                                aCTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagcca                                                                                                                                               >  1:216826/1‑88 (MQ=255)                                                                aCTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagcca                                                                                                                                               <  2:216826/88‑1 (MQ=255)                                                                 cTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcg                                                                                 <  7:335614/149‑1 (MQ=255)                                                                 cTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcg                                                                                 <  7:329697/149‑1 (MQ=255)                                                                  tGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACgg                                                                                                   >  3:271354/1‑130 (MQ=255)                                                                  tGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACgg                                                                                                   <  4:271354/130‑1 (MQ=255)                                                                  tGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTtctc                                                                                       >  1:311978/1‑142 (MQ=255)                                                                  tGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTtctc                                                                                       <  2:311978/142‑1 (MQ=255)                                                                     cGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcgcggc                                                                             <  8:278682/149‑1 (MQ=255)                                                                         gAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACgg                                                                                                        <  7:138246/118‑1 (MQ=255)                                                                         gAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACgg                                                                                                        >  8:138246/1‑118 (MQ=255)                                                                         gAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGAt                                                                         >  8:72796/1‑149 (MQ=255)                                                                             gtggtgGCCGCCTGGGCGGGGGCCGGTGGTAACGCGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCCGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTAc                                                                                                     >  1:311147/1‑117 (MQ=255)                                                                               ggtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTAttt                                                                                          >  7:151658/1‑126 (MQ=255)                                                                               ggtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTAttt                                                                                          <  8:151658/126‑1 (MQ=255)                                                                               ggtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgg                                                                   >  2:196610/1‑149 (MQ=255)                                                                                  ggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCggggg                                                                >  8:115508/1‑149 (MQ=255)                                                                                     cgccTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggcgg                                                             >  4:290718/1‑149 (MQ=255)                                                                                      gccTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggcgg                                                             >  4:172764/1‑148 (MQ=255)                                                                                              gggggCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAg                                                    >  3:168903/1‑149 (MQ=255)                                                                                                   ccGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAg                                               <  3:290718/149‑1 (MQ=255)                                                                                                    cGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCa                                                >  4:246615/1‑147 (MQ=255)                                                                                                    cGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCa                                                <  3:246615/147‑1 (MQ=255)                                                                                                    cGGTGGTAACGGGCGGCTGCTGGCCACAAACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAgg                                              >  1:233396/1‑149 (MQ=255)                                                                                                       tggtAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAg                                           <  8:176681/149‑1 (MQ=255)                                                                                                         gtAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCg                                         <  1:196610/149‑1 (MQ=255)                                                                                                                  ggcTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAgggg                                                                                                                        >  1:323325/1‑60 (MQ=255)                                                                                                                  ggcTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAggg                                                                                                                         <  2:323325/60‑1 (MQ=255)                                                                                                                    ctgctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCgg                              <  7:72796/149‑1 (MQ=255)                                                                                                                     tgctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGc                             >  1:272558/1‑149 (MQ=255)                                                                                                                     tgctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGc                             >  2:38192/1‑149 (MQ=255)                                                                                                                        tgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggc                                                               >  8:274127/1‑112 (MQ=255)                                                                                                                        tgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggc                                                               <  7:274127/112‑1 (MQ=255)                                                                                                                          gCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAgg                                              >  4:24050/1‑127 (MQ=255)                                                                                                                            cacaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAg                                               >  6:336385/1‑124 (MQ=255)                                                                                                                            cacaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAg                                               <  5:336385/124‑1 (MQ=255)                                                                                                                            cacaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtg                      >  7:347802/1‑149 (MQ=255)                                                                                                                             acaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCgtggtggt                     <  4:168903/149‑8 (MQ=255)                                                                                                                             acaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtgg                     >  4:51120/1‑149 (MQ=255)                                                                                                                               aTACGGGTTGCTGCCCTGGCAGACAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGTTAGCGGCCAAGCCCGGCGAAggtgggg                   >  3:104786/1‑149 (MQ=255)                                                                                                                                 aCGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggggtg                 >  5:355311/1‑149 (MQ=255)                                                                                                                                  cGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggggtgg                <  3:51120/149‑1 (MQ=255)                                                                                                                                       cattgcCCTGGCTGGCTGCCAGGGCAGTGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCTAAGGTGGGGTGGCGACg           >  3:129469/4‑149 (MQ=255)                                                                                                                                       cattgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACg           >  4:109777/4‑149 (MQ=255)                                                                                                                                        attgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGaa                          >  8:125133/3‑133 (MQ=255)                                                                                                                                        attgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGaa                          <  7:125133/131‑1 (MQ=255)                                                                                                                                        attgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggg                    <  3:317974/137‑1 (MQ=255)                                                                                                                                        attgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggg                    >  4:317974/3‑139 (MQ=255)                                                                                                                                         ctgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGt         <  2:233396/149‑1 (MQ=255)                                                                                                                                          tgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgggg                                                                 <  1:224425/92‑1 (MQ=255)                                                                                                                                          tgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgggg                                                                 >  2:224425/1‑92 (MQ=255)                                                                                                                                            cccTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg       <  3:109777/148‑1 (MQ=255)                                                                                                                                            cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTggg      >  6:175278/1‑149 (MQ=255)                                                                                                                                            cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTggg      >  6:52935/1‑149 (MQ=255)                                                                                                                                            cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg       <  1:38192/148‑1 (MQ=255)                                                                                                                                            cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg       <  5:204346/148‑1 (MQ=255)                                                                                                                                            cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGACCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTggg      >  7:238400/1‑149 (MQ=255)                                                                                                                                                ggCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAg  >  3:339973/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CTGGCCGTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAG  >  NC_002947/675441‑675732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑