Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	675,591	A→T	5.9%	A36A (GCT→GCA)	PP_0577 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	675,591	0	A	T	5.9%	250.4 / 3.2	80	A36A (GCT→GCA)	PP_0577	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (40/35);  new base T (3/2);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTCC  >  NC_002947/675450‑675736                                                                                                                                              |                                                                                                                                                  gcgcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAgg                                                                                                                                             <  3:350578/148‑1 (MQ=255) gcgcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAgg                                                                                                                                             <  3:328539/148‑1 (MQ=255) gcgcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGGAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAgg                                                                                                                                             <  3:119544/148‑1 (MQ=255)  cgcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAggg                                                                                                                                            <  8:191797/148‑1 (MQ=255)   gcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCGACATACGGGTTGCTGCCCTGGCAGCCAGCCAgggga                                                                                                                                          >  3:302644/1‑147 (MQ=255)    ccagctccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCaa                                                                                                                                         >  8:335614/1‑149 (MQ=255)    ccagctccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCaa                                                                                                                                         >  8:329697/1‑149 (MQ=255)         tccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATggg                                                                                                                                     >  7:288070/1‑148 (MQ=255)             gctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGgcagca                                                                                                                                <  7:323867/149‑1 (MQ=255)             gctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGgcagca                                                                                                                                <  5:231812/149‑1 (MQ=255)              ctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGcagcag                                                                                                                               <  7:212073/149‑1 (MQ=255)               tGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGccagccag                                                                                                                                              >  6:41513/1‑133 (MQ=255)               tGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGccagccag                                                                                                                                              <  5:41513/133‑1 (MQ=255)                 cGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAggg                                                                                                                             >  3:87898/1‑148 (MQ=255)                         cccATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGcac                                                                                                                     >  1:171038/1‑148 (MQ=255)                         cccATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGcac                                                                                                                     >  3:219324/1‑148 (MQ=255)                           cATAATAACGAGCGGGCGCATGGTAAGCACTGGGGTGGAAGGTGGTGGCCGCCTGGGGGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATTGGCAGCAGGGCCAGGCacaac                                                                                                                  <  4:87898/149‑1 (MQ=255)                            aTAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCacaa                                                                                                                   >  7:278682/1‑147 (MQ=255)                             tAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCacaa                                                                                                                   >  5:150938/1‑146 (MQ=255)                                 cACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACg                                                                                                             <  8:288070/148‑1 (MQ=255)                                     aGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACg                                                                                                        <  2:171038/149‑1 (MQ=255)                                           cgcAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGt                                                                                                  <  5:361762/149‑1 (MQ=255)                                           cgcAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGt                                                                                                  <  4:219324/149‑1 (MQ=255)                                            gcATGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGc                                                                                                      >  4:13235/1‑144 (MQ=255)                                            gcAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGc                                                                                                      <  3:13235/144‑1 (MQ=255)                                                     gCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcg                                                                                        >  7:176681/1‑149 (MQ=255)                                                       aCTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagcca                                                                                                                                                   <  2:216826/88‑1 (MQ=255)                                                       aCTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagcca                                                                                                                                                   >  1:216826/1‑88 (MQ=255)                                                        cTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcg                                                                                     <  7:329697/149‑1 (MQ=255)                                                        cTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcg                                                                                     <  7:335614/149‑1 (MQ=255)                                                         tGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACgg                                                                                                       >  3:271354/1‑130 (MQ=255)                                                         tGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACgg                                                                                                       <  4:271354/130‑1 (MQ=255)                                                         tGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTtctc                                                                                           <  2:311978/142‑1 (MQ=255)                                                         tGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTtctc                                                                                           >  1:311978/1‑142 (MQ=255)                                                            cGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcgcggc                                                                                 <  8:278682/149‑1 (MQ=255)                                                                gAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACgg                                                                                                            <  7:138246/118‑1 (MQ=255)                                                                gAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACgg                                                                                                            >  8:138246/1‑118 (MQ=255)                                                                gAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGAt                                                                             >  8:72796/1‑149 (MQ=255)                                                                    gtggtgGCCGCCTGGGCGGGGGCCGGTGGTAACGCGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCCGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTAc                                                                                                         >  1:311147/1‑117 (MQ=255)                                                                      ggtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTAttt                                                                                              <  8:151658/126‑1 (MQ=255)                                                                      ggtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTAttt                                                                                              >  7:151658/1‑126 (MQ=255)                                                                      ggtggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgg                                                                       >  2:196610/1‑149 (MQ=255)                                                                         ggCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCggggg                                                                    >  8:115508/1‑149 (MQ=255)                                                                            cgccTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggcgg                                                                 >  4:290718/1‑149 (MQ=255)                                                                             gccTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggcgg                                                                 >  4:172764/1‑148 (MQ=255)                                                                                     gggggCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAg                                                        >  3:168903/1‑149 (MQ=255)                                                                                          ccGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAg                                                   <  3:290718/149‑1 (MQ=255)                                                                                           cGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCa                                                    >  4:246615/1‑147 (MQ=255)                                                                                           cGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCa                                                    <  3:246615/147‑1 (MQ=255)                                                                                           cGGTGGTAACGGGCGGCTGCTGGCCACAAACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAgg                                                  >  1:233396/1‑149 (MQ=255)                                                                                              tggtAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAg                                               <  8:176681/149‑1 (MQ=255)                                                                                                gtAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCg                                             <  1:196610/149‑1 (MQ=255)                                                                                                         ggcTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAgggg                                                                                                                            >  1:323325/1‑60 (MQ=255)                                                                                                         ggcTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAggg                                                                                                                             <  2:323325/60‑1 (MQ=255)                                                                                                           ctgctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCgg                                  <  7:72796/149‑1 (MQ=255)                                                                                                            tgctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGc                                 >  1:272558/1‑149 (MQ=255)                                                                                                            tgctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGc                                 >  2:38192/1‑149 (MQ=255)                                                                                                               tgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggc                                                                   >  8:274127/1‑112 (MQ=255)                                                                                                               tgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggc                                                                   <  7:274127/112‑1 (MQ=255)                                                                                                                 gCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAgg                                                  >  4:24050/1‑127 (MQ=255)                                                                                                                   cacaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAg                                                   <  5:336385/124‑1 (MQ=255)                                                                                                                   cacaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAg                                                   >  6:336385/1‑124 (MQ=255)                                                                                                                   cacaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtg                          >  7:347802/1‑149 (MQ=255)                                                                                                                    acaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCgtggtggt                         <  4:168903/149‑8 (MQ=255)                                                                                                                    acaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtgg                         >  4:51120/1‑149 (MQ=255)                                                                                                                      aTACGGGTTGCTGCCCTGGCAGACAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGTTAGCGGCCAAGCCCGGCGAAggtgggg                       >  3:104786/1‑149 (MQ=255)                                                                                                                        aCGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggggtg                     >  5:355311/1‑149 (MQ=255)                                                                                                                         cGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggggtgg                    <  3:51120/149‑1 (MQ=255)                                                                                                                              cattgcCCTGGCTGGCTGCCAGGGCAGTGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCTAAGGTGGGGTGGCGACg               >  3:129469/4‑149 (MQ=255)                                                                                                                              cattgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACg               >  4:109777/4‑149 (MQ=255)                                                                                                                               attgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGaa                              <  7:125133/131‑1 (MQ=255)                                                                                                                               attgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGaa                              >  8:125133/3‑133 (MQ=255)                                                                                                                               attgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggg                        <  3:317974/137‑1 (MQ=255)                                                                                                                               attgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggg                        >  4:317974/3‑139 (MQ=255)                                                                                                                                ctgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGt             <  2:233396/149‑1 (MQ=255)                                                                                                                                 tgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgggg                                                                     >  2:224425/1‑92 (MQ=255)                                                                                                                                 tgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCgggg                                                                     <  1:224425/92‑1 (MQ=255)                                                                                                                                   cccTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg           <  3:109777/148‑1 (MQ=255)                                                                                                                                   cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTggg          >  6:52935/1‑149 (MQ=255)                                                                                                                                   cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTggg          >  6:175278/1‑149 (MQ=255)                                                                                                                                   cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg           <  5:204346/148‑1 (MQ=255)                                                                                                                                   cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg           <  1:38192/148‑1 (MQ=255)                                                                                                                                   cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGACCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTggg          >  7:238400/1‑149 (MQ=255)                                                                                                                                       ggCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAg      >  3:339973/1‑149 (MQ=255)                                                                                                                                           gccagccaGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTcc  <  7:115508/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  GCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTCC  >  NC_002947/675450‑675736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑