Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 707,565 G→A 5.2% intergenic (‑217/‑34) rpsT ← / → murJ 30S ribosomal protein S20/lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947707,5650GA 5.2% 297.2 / 3.4 96intergenic (‑217/‑34)rpsT/murJ30S ribosomal protein S20/lipid II flippase
Reads supporting (aligned to +/- strand):  ref base G (43/47);  new base A (2/3);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

GGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTCG  >  NC_002947/707421‑707707
                                                                                                                                                |                                                                                                                                              
ggATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCTGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGc                                                                                                                                            <  3:146182/149‑1 (MQ=255)
 gATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCg                                                                                                                                           >  8:286068/1‑149 (MQ=255)
 gATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCg                                                                                                                                           >  5:193073/1‑149 (MQ=255)
 gATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGCCAGACAGCg                                                                                                                                           >  4:208219/1‑149 (MQ=255)
   tGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGCCAGACAGCGTc                                                                                                                                         <  7:390267/149‑1 (MQ=255)
   tGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGCCAGACAGCGTc                                                                                                                                         <  3:208219/149‑1 (MQ=255)
    gTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTcc                                                                                                                                        >  3:18359/1‑149 (MQ=255)
     tCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCct                                                                                                                                       <  7:46447/149‑1 (MQ=255)
     tCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCct                                                                                                                                       >  7:40419/1‑149 (MQ=255)
     tCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGCCAGACAGCGTcc                                                                                                                                        <  2:365913/148‑1 (MQ=255)
      cAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCctc                                                                                                                                      >  4:235814/1‑149 (MQ=255)
      cAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGGACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCctc                                                                                                                                      >  5:349439/1‑149 (MQ=255)
       aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCctct                                                                                                                                     >  3:297312/1‑149 (MQ=255)
       aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGCCACCCCTGCGAAGGCGCGGCAGTATCGCACATTCGGACGCTGAGTGGCAGCGTCctct                                                                                                                                     >  4:311012/1‑149 (MQ=255)
        aGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTg                                                                                                                                    >  3:223836/1‑149 (MQ=255)
         ggCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGt                                                                                                                                   >  2:357203/1‑149 (MQ=255)
         ggCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGt                                                                                                                                   >  4:351563/1‑149 (MQ=255)
          gCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTc                                                                                                                                  <  2:366106/149‑1 (MQ=255)
           ccACCTGCAAGGTTCCGCCGAGCGGTGCTCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCa                                                                                                                                 <  7:28018/149‑1 (MQ=255)
              ccTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTg                                                                                                                              <  2:119353/149‑1 (MQ=255)
               cTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTgg                                                                                                                             >  2:124770/1‑149 (MQ=255)
               cTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTgg                                                                                                                             <  8:329932/149‑1 (MQ=255)
                  cAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGc                                                                                                                            <  8:40419/147‑1 (MQ=255)
                  cAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGt                                                                                                                          <  5:31164/149‑1 (MQ=255)
                  cAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGt                                                                                                                          <  2:217108/149‑1 (MQ=255)
                  cAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGt                                                                                                                          <  3:235814/149‑1 (MQ=255)
                   aaGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGt                                                                                                                          >  4:308312/1‑148 (MQ=255)
                    aGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGa                                                                                                                        <  4:164713/149‑1 (MQ=255)
                    aGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGa                                                                                                                        <  3:351563/149‑1 (MQ=255)
                           gccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTGCTCTGTCATTGGCGTGAATCtttt                                                                                                                 >  5:425730/1‑149 (MQ=255)
                            ccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTc                                                                                                                <  5:260573/149‑1 (MQ=255)
                            ccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTc                                                                                                                <  4:18359/149‑1 (MQ=255)
                                   gTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAAc                                                                                                         <  3:308312/149‑1 (MQ=255)
                                             aaGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAAt                                                                                               <  7:286068/149‑1 (MQ=255)
                                                cGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATccc                                                                                            <  4:326575/149‑1 (MQ=255)
                                                 gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                           <  1:124770/149‑1 (MQ=255)
                                                 gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                           <  4:297312/149‑1 (MQ=255)
                                                  ggAAAGATTCCTTTCTGCTGCGCGTCCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTg                                                                                          <  3:311012/149‑1 (MQ=255)
                                                    aaaGAATCCGTTCTGCCGCGCGACCTGTACACTCCGGAAATTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGc                                                                                        <  1:87748/149‑1 (MQ=255)
                                                      aGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTg                                                                                      >  1:195578/1‑149 (MQ=255)
                                                         ttCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCTCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCt                                                                                       <  4:36531/145‑1 (MQ=255)
                                                         ttCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACATGCTCAAATCCCTGGCt                                                                                       >  3:36531/1‑145 (MQ=255)
                                                          tCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAg                                                                                   >  5:114849/1‑148 (MQ=255)
                                                          tCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGCCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTAAAATCCCTCGCTGCAg                                                                                   >  6:328060/1‑148 (MQ=255)
                                                                ctgctgCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTc                                                                            >  5:399798/1‑149 (MQ=255)
                                                                 tgctgcGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                 >  1:243904/1‑111 (MQ=255)
                                                                 tgctgcGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                 <  2:243904/111‑1 (MQ=255)
                                                                 tgctgcGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTc                                                                            >  1:214606/1‑148 (MQ=255)
                                                                  gctgcGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGa                                                                          >  1:208729/1‑149 (MQ=255)
                                                                   ctgcGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGa                                                                          <  6:114849/148‑1 (MQ=255)
                                                                       gcgACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCAcc                                                                     <  2:195578/149‑1 (MQ=255)
                                                                         gACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCAt                                                                   <  2:208729/149‑1 (MQ=255)
                                                                            cTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGAt                                                                >  2:185981/1‑149 (MQ=255)
                                                                                 cacTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTcgc                                                           >  6:14781/1‑149 (MQ=255)
                                                                                 cacTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACATGCTCAAATCAATGGCTGCAGTAAGCTCGATCACCATGATTTcgc                                                           >  2:364284/1‑149 (MQ=255)
                                                                                  acTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTCCGCTAGAGCACCAAGCTTTcgcg                                                          >  6:290746/1‑149 (MQ=255)
                                                                                   cTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCgg                                                         <  6:193073/149‑1 (MQ=255)
                                                                                    tCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGc                                                                                                                                            >  2:297789/1‑65 (MQ=255)
                                                                                    tCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGc                                                                                                                                            <  1:297789/65‑1 (MQ=255)
                                                                                      gggAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTg                                                      <  1:357203/149‑1 (MQ=255)
                                                                                      gggAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTg                                                      >  5:201900/1‑149 (MQ=255)
                                                                                         aaTTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTg                                                   >  1:399121/1‑149 (MQ=255)
                                                                                         aaTTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTg                                                   <  1:185981/149‑1 (MQ=255)
                                                                                          aTTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTgg                                                  <  5:14781/149‑1 (MQ=255)
                                                                                                ggCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCttt                                             <  4:223836/148‑1 (MQ=255)
                                                                                                ggCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTg                                            >  7:211932/1‑149 (MQ=255)
                                                                                                   tCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCg                                                                           >  4:170364/1‑115 (MQ=255)
                                                                                                   tCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCg                                                                           <  3:170364/115‑1 (MQ=255)
                                                                                                   tCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTc                                         >  7:11191/1‑149 (MQ=255)
                                                                                                     cccTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTcgc                                       >  6:92737/1‑149 (MQ=255)
                                                                                                     cccTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTcg                                        <  8:211932/148‑1 (MQ=255)
                                                                                                       cTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGa                                     <  2:399121/149‑1 (MQ=255)
                                                                                                          cGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCa                                                                    >  8:163906/1‑115 (MQ=255)
                                                                                                              ggCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATc                              <  1:220798/149‑1 (MQ=255)
                                                                                                                     gAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATcc                             <  2:263574/143‑1 (MQ=255)
                                                                                                                     gAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATcc                             >  1:263574/1‑143 (MQ=255)
                                                                                                                     gAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGccc                       >  5:287410/1‑149 (MQ=255)
                                                                                                                            gCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCtt                >  2:55916/1‑149 (MQ=255)
                                                                                                                             cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCttt               <  6:402545/149‑1 (MQ=255)
                                                                                                                             cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCttt               >  1:133729/1‑149 (MQ=255)
                                                                                                                              acaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGa                                                                          <  5:54070/89‑1 (MQ=255)
                                                                                                                              acaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGa                                                                          >  6:54070/1‑89 (MQ=255)
                                                                                                                              acaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTg              <  1:364284/149‑1 (MQ=255)
                                                                                                                                aTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                           <  6:378625/70‑1 (MQ=255)
                                                                                                                                aTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                           >  5:378625/1‑70 (MQ=255)
                                                                                                                                   aggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCt         >  5:31642/2‑149 (MQ=255)
                                                                                                                                    ggAGGCTGTCTGGCAGCGTCCTCTGTCATTCGCGGGAATCTTTTCGATGAACCTGCGCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGGCCGCATCTTTGGTGCTg        <  6:425730/149‑1 (MQ=255)
                                                                                                                                    ggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCGAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTg        >  5:237803/1‑149 (MQ=255)
                                                                                                                                    ggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTg        <  6:399798/149‑1 (MQ=255)
                                                                                                                                     gACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTgg       <  5:92737/149‑1 (MQ=255)
                                                                                                                                     gACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTgg       >  8:327021/1‑149 (MQ=255)
                                                                                                                                       cggtGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCTATGCACCTGCTCAAATCGCTGGCTGCAGTAAGCTCGATCAGCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGgtg     <  5:328060/146‑1 (MQ=255)
                                                                                                                                       cGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGgtg     <  2:214606/149‑1 (MQ=255)
                                                                                                                                        gCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGgtgt    <  8:11191/149‑1 (MQ=255)
                                                                                                                                        gCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGgtgt    >  3:26538/1‑149 (MQ=255)
                                                                                                                                          tGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTCg  >  2:288364/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTCG  >  NC_002947/707421‑707707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑