Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	733,645	A→T	15.4%	intergenic (+19/‑28)	PP_t09 → / → PP_t10	tRNA‑Lys/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	733,645	0	A	T	15.4%	190.0 / 23.5	78	intergenic (+19/‑28)	PP_t09/PP_t10	tRNA‑Lys/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base A (34/32);  new base T (7/5);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTT  >  NC_002947/733499‑733790                                                                                                                                                   |                                                                                                                                                   cTTTGTGAGCATGATGTGAGTAGTCTGAATCCCCTCACCCCTACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                  <  8:315793/149‑1 (MQ=17) cTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                  <  3:198013/149‑1 (MQ=255)  tttGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACAATTTTACGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAag                                                                                                                                                 >  4:193147/1‑149 (MQ=255)    tGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAaga                                                                                                                                                <  4:286036/148‑1 (MQ=255)        aGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGc                                                                                                                                           <  8:247861/149‑1 (MQ=255)          cAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGccc                                                                                                                                         <  6:21384/149‑1 (MQ=255)          cAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGcc                                                                                                                                          <  3:243138/148‑1 (MQ=255)                gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGAt                                                                                                                                             <  4:297789/139‑1 (MQ=255)                gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGAt                                                                                                                                             >  3:297789/1‑139 (MQ=255)                gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tttttt                                                                                                                                  >  7:173258/1‑149 (MQ=255)                 tCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑ttttttt                                                                                                                                 >  1:405951/1‑149 (MQ=255)                           gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTc                                                                                                                       >  1:178144/1‑149 (MQ=255)                             aTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCgg                                                                                                                     <  1:169657/149‑1 (MQ=255)                              tCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCggg                                                                                                                    <  8:173258/149‑1 (MQ=255)                                 ccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGCCC‑t                                                                                                                                       <  5:282308/127‑1 (MQ=255)                                 ccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGCCC‑t                                                                                                                                       >  6:282308/1‑127 (MQ=255)                                  cTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGgtg                                                                                                                 >  4:188995/1‑148 (MQ=255)                                       cccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tttttttt                                                                                                                                >  5:76081/1‑128 (MQ=255)                                       cccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tttttttt                                                                                                                                <  6:76081/128‑1 (MQ=255)                                        ccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTTGAAACACTCGGGGTGTAgcg                                                                                                            >  6:166426/1‑148 (MQ=255)                                              aTTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTACgg                                                                                                    >  6:125352/1‑149 (MQ=255)                                                  tCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGccc                                                                                                                                         >  1:110299/1‑109 (MQ=255)                                                  tCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGccc                                                                                                                                         <  2:110299/109‑1 (MQ=255)                                                    cGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAgcgc                                                                                              >  7:331284/1‑149 (MQ=255)                                                     gggTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCTAATCCTACACGTCCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCgg                                                                                                                     <  8:103966/125‑1 (MQ=255)                                                     gggTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCgg                                                                                                                     >  7:103966/1‑125 (MQ=255)                                                               cTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                   <  3:188995/149‑1 (MQ=255)                                                                tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                  >  2:137751/1‑86 (MQ=255)                                                                tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCaaa                                                                                                                                                  <  1:137751/86‑1 (MQ=255)                                                                tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                   >  3:164658/1‑148 (MQ=255)                                                                tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                   >  4:370714/1‑148 (MQ=255)                                                                  aGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                   <  5:166426/147‑1 (MQ=255)                                                                  aGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGa                                                                                 <  5:125352/148‑1 (MQ=255)                                                                  aGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAg                                                                                >  3:165790/1‑149 (MQ=255)                                                                   gTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTg                                                                                    >  4:184185/1‑144 (MQ=255)                                                                   gTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTg                                                                                    <  3:184185/144‑1 (MQ=255)                                                                    ttGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tttttttt                                                                                                                                <  1:161646/99‑1 (MQ=255)                                                                    ttGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tttttttt                                                                                                                                >  2:161646/1‑99 (MQ=255)                                                                    ttGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCa                                                                              <  2:405951/149‑1 (MQ=255)                                                                      ggTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAg                                                                             <  8:331284/148‑1 (MQ=255)                                                                       gTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGa                                                                           >  6:5312/1‑149 (MQ=255)                                                                        tAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGAt                                                                          <  2:178144/149‑1 (MQ=255)                                                                            gCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTc                                                                       <  4:165790/148‑1 (MQ=255)                                                                            gCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCa                                                                      <  3:193147/149‑1 (MQ=255)                                                                              aGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAgg                                                                    <  5:5312/149‑1 (MQ=255)                                                                                     ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTggg                                                                                  <  8:65587/128‑1 (MQ=255)                                                                                     ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTggg                                                                                  >  7:65587/1‑128 (MQ=255)                                                                                     ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTACAAAAGGCAAAAACAAAGAGATGCCC‑TTTTATTTGAAACACTAGGGGTGTAGCGAAGTGCGAGAGCGCGCATGCTTTGGGAGGAGGATGTCAGGAGttag                                                              >  4:176162/1‑146 (MQ=21)                                                                                            cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                   <  4:4637/120‑1 (MQ=255)                                                                                            cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                   >  3:4637/1‑120 (MQ=255)                                                                                             aaTTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTc                                                     >  7:315269/1‑149 (MQ=255)                                                                                              aTTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCa                                                    <  4:164658/149‑1 (MQ=255)                                                                                                     gTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGa                                              <  8:315269/148‑1 (MQ=255)                                                                                                       aGGTTAGAATCCTACACGACCCACCATGTAGAAAATGGCATCTCAAAGAGATGCCC‑TTTTTAATGAAACAATCGTGGTGTAGCGCAGTCCGGTAGCGCGCATGCTTTGGGAGAACGATGTAAGGAGTTCGATTCCCATCCACACGACCa                                           >  5:225094/1‑149 (MQ=21)                                                                                                           tCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAttt                                        >  3:34088/1‑149 (MQ=255)                                                                                                           tCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCa                                                                      <  5:283124/118‑1 (MQ=255)                                                                                                           tCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCa                                                                      >  6:283124/1‑118 (MQ=255)                                                                                                                   tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                               >  7:291822/1‑149 (MQ=255)                                                                                                                   tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                               >  7:147250/1‑149 (MQ=255)                                                                                                                   tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                               >  5:128705/1‑149 (MQ=255)                                                                                                                           ccaccaTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAg                       >  5:210133/1‑149 (MQ=255)                                                                                                                             accaTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTc                                                               >  8:154829/1‑107 (MQ=255)                                                                                                                             accaTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTc                                                               <  7:154829/107‑1 (MQ=255)                                                                                                                             accaTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGtt                     >  2:194555/1‑149 (MQ=255)                                                                                                                               caTGTATAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGt                      <  3:217364/146‑1 (MQ=255)                                                                                                                               caTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGTAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGt                      >  4:217364/1‑146 (MQ=255)                                                                                                                                 tGTAGAAAAGGGCATCTCAACGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                 <  1:194555/149‑1 (MQ=255)                                                                                                                                   tAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                 >  2:171769/1‑147 (MQ=255)                                                                                                                                   agatctaGGGCATCTGTTTGAGATGGCG‑TTTTTTTTGATACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTaga               <  4:33114/143‑1 (MQ=17)                                                                                                                                    aGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTagag              >  8:336383/1‑149 (MQ=255)                                                                                                                                        aaGGGCATCTCTTTGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGt          >  1:55307/1‑149 (MQ=255)                                                                                                                                         aGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGtt         <  8:291822/149‑1 (MQ=255)                                                                                                                                         aGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGtt         <  8:147250/149‑1 (MQ=255)                                                                                                                                          gggCATCTCTTTGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTg                    >  5:350084/1‑137 (MQ=255)                                                                                                                                          gggCATCTCTTTGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTg                    <  6:350084/137‑1 (MQ=255)                                                                                                                                          gggCATCTCTTTGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg        >  6:48808/1‑149 (MQ=255)                                                                                                                                          gggCATCTCTTTGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTAACCCCGACCATTTTTGGGTCGTTAGCTCACTTGGTagagagatag        >  3:33114/1‑143 (MQ=17)                                                                                                                                           ggCATCTCTTTGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg       >  2:182226/1‑149 (MQ=255)                                                                                                                                           ggCATCTCAAAGAGATGCCC‑TTTTTTTTGGAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                 >  1:382960/1‑139 (MQ=255)                                                                                                                                           ggCATCTCAAAGAGATGCCC‑TTTTTTTTGGAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                 <  2:382960/139‑1 (MQ=255)                                                                                                                                           ggCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg       <  6:128705/149‑1 (MQ=255)                                                                                                                                           ggCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg       <  7:336383/149‑1 (MQ=255)                                                                                                                                                   aaaGAGATGCGC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGTTAGAGCAGTTGGCtttt  <  6:225094/146‑1 (MQ=255)                                                                                                                                                   aaaGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTg                                     >  7:368346/1‑111 (MQ=255)                                                                                                                                                   aaaGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTg                                     <  8:368346/111‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTT  >  NC_002947/733499‑733790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑