Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 188,613 T→C 8.6% L241P (CTG→CCG)  PP_0165 → GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947188,6130TC 8.6% 157.9 / 5.2 58L241P (CTG→CCG) PP_0165GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (28/25);  new base C (2/3);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

CAGCCTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCG  >  NC_002947/188470‑188759
                                                                                                                                               |                                                                                                                                                  
cAGCCTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCg                                                                                                                                               >  4:322812/1‑149 (MQ=255)
  gCCTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCggg                                                                                                                                             <  6:132229/149‑1 (MQ=255)
  gCCTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCggg                                                                                                                                             <  7:153041/149‑1 (MQ=255)
       ccAGAGTTGCCACGCACGCCGGAGCTCCGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGt                                                                                                                                         <  3:332107/148‑1 (MQ=255)
       ccAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGt                                                                                                                                         <  2:70286/148‑1 (MQ=255)
             ttGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCCGGTCAGGCTGTcctggta                                                                                                                                  <  3:302780/149‑7 (MQ=255)
             ttGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCCGGTCAGGCTGTcctggta                                                                                                                                  <  3:740/149‑7 (MQ=255)
               gCCACGCACGCCGGAGCTACGCTGCGTAGCGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAAccgc                                                                                                                                 <  2:126956/148‑1 (MQ=255)
               gCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAAccgc                                                                                                                                 <  8:369640/148‑1 (MQ=255)
                 cacgcacgCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCCGGTCAGGCTGTcctg                                                                                                                                     <  5:316447/142‑4 (MQ=255)
                 cacgcacgCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCCGGTCAGGCTGTcctg                                                                                                                                     >  6:316447/1‑139 (MQ=255)
                      acgcCGTAGCTACGCCGCGTAGTGCAAGCGAGGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTAttt                                                                                                                         <  3:322812/149‑1 (MQ=255)
                        gcCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTAtttt                                                                                                                        >  8:102968/1‑148 (MQ=255)
                        gacGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCACTGTTCAACGATCAGGCAGAGAGAAGTGAGCGGCTTCGTGCGGAGTCCTACCAGGACAGCCTGAACGGCCAGACGAACCGCAGCTAtttt                                                                                                                        >  6:30071/3‑148 (MQ=255)
                              gCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATg                                                                                                                  >  5:58539/1‑148 (MQ=255)
                                     cgcgTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCa                                                                                                                <  7:292797/143‑1 (MQ=255)
                                     cgcgTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCa                                                                                                                >  8:292797/1‑143 (MQ=255)
                                          aGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATc                                                                                                     >  8:126919/1‑149 (MQ=255)
                                                   gATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCggg                                                                                                                                             >  1:190574/1‑100 (MQ=255)
                                                   gATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCggg                                                                                                                                             <  2:190574/100‑1 (MQ=255)
                                                   gATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGc                                                                                             >  6:405520/1‑148 (MQ=255)
                                                    aTGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCgg                                                                                                   <  7:96006/141‑1 (MQ=255)
                                                    aTGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCgg                                                                                                   >  8:96006/1‑141 (MQ=255)
                                                       aaCCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACa                                                                                                        >  8:354239/1‑133 (MQ=255)
                                                       aaCCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACa                                                                                                        <  7:354239/133‑1 (MQ=255)
                                                            gATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTgg                                                                                      >  3:226693/1‑146 (MQ=255)
                                                                gTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGACTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGAcgc                                                                               >  5:289582/1‑149 (MQ=255)
                                                                 tCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCt                                                                                                             <  6:96872/118‑1 (MQ=255)
                                                                 tCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCt                                                                                                             >  5:96872/1‑118 (MQ=255)
                                                                     gaAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGAcgcgcgcg                                                                          >  1:45104/1‑149 (MQ=255)
                                                                             aaGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTAc                                                                  >  2:271111/1‑149 (MQ=255)
                                                                             aaGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTAc                                                                  <  6:58539/149‑1 (MQ=255)
                                                                              aGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTAcc                                                                 >  2:412255/1‑149 (MQ=255)
                                                                                cccTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTAttt                                                                                                                         >  1:110075/1‑91 (MQ=255)
                                                                                cccTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTAttt                                                                                                                         <  2:110075/91‑1 (MQ=255)
                                                                                  cTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGAcgc                                                                               <  4:44234/131‑1 (MQ=255)
                                                                                  cTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGAcgc                                                                               >  3:44234/1‑131 (MQ=255)
                                                                                        aCCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTa                                                                                                <  4:89025/108‑1 (MQ=255)
                                                                                        aCCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTtgct                                                       >  1:56773/1‑149 (MQ=255)
                                                                                          cGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTtgctgc                                                     <  2:149662/149‑1 (MQ=255)
                                                                                             gCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCggg                                                  <  5:405520/149‑1 (MQ=255)
                                                                                                 gCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCa                                              <  7:126919/149‑1 (MQ=255)
                                                                                                   cGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAgg                                            >  8:255610/1‑149 (MQ=255)
                                                                                                         cAGTGAGCGGCTGCGTTCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCAGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTTCTTTTGCTGCGGGTTCAGGGCctgg                                      <  5:30071/149‑1 (MQ=255)
                                                                                                         cAGTGAGCGGCTGCGTGCTGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCctgg                                      <  2:2619/149‑1 (MQ=255)
                                                                                                            tGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCctggctg                                   <  2:56773/149‑1 (MQ=255)
                                                                                                              aGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCtggctggc                                 <  1:412255/149‑1 (MQ=255)
                                                                                                               gCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTtgc                                                        <  6:321662/125‑1 (MQ=255)
                                                                                                               gCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTtgc                                                        >  5:321662/1‑125 (MQ=255)
                                                                                                                cGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCt                               >  6:274145/1‑149 (MQ=255)
                                                                                                                                 gcggttcgACAGCCCGGTCAGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcg              >  5:106060/8‑149 (MQ=255)
                                                                                                                                 cTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcg              >  7:5947/1‑149 (MQ=255)
                                                                                                                                  tACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcgg             >  5:117025/1‑149 (MQ=255)
                                                                                                                                   aCCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTggcggc            <  7:102968/149‑1 (MQ=255)
                                                                                                                                        gACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCg       >  8:132591/1‑149 (MQ=255)
                                                                                                                                             ccTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTAcc   >  3:221517/1‑148 (MQ=255)
                                                                                                                                             ccTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTAcc   >  6:198660/1‑148 (MQ=255)
                                                                                                                                             ccTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCg  >  8:114555/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CAGCCTGCCAGAGTTGCCACGCACGCCGGAGCTACGCCGCGTAGTGCAAGCGATGAACCAGATGGTCGAGAAACTCAAAGCCCTGTTCACCGAGCAGGCCGAGCGCAGTGAGCGGCTGCGTGCGGAGTCCTACCAGGACAGCCTGACCGGGCTGTCGAACCGCCGCTATTTTGAAATGCAGCTGAACAATCGGGTAAGCAACCTGGAAGACGCGCGCGCCGGTTACCTGCTGTTGCTGCGGGTTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCG  >  NC_002947/188470‑188759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑