Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 799,761 G→T 12.4% L238M (CTG→ATG)  ispB ← octaprenyl diphosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947799,7610GT12.4% 144.1 / 2.1 52L238M (CTG→ATG) ispBoctaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (17/28);  new base T (5/2);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCGGCGGTGTATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGC  >  NC_002947/799617‑799900
                                                                                                                                                |                                                                                                                                           
gCTCGGCGGTGTATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAggg                                                                                                                                           >  1:363230/1‑147 (MQ=255)
gCTCGGCGGTGTATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCACCGTCAgcgaa                                                                                                                                         >  8:209422/1‑145 (MQ=255)
 cTCGGCGGTGTATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAg                                                                                                                                        <  2:363230/149‑1 (MQ=255)
      cggTGTATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTg                                                                                                                                   <  4:297502/149‑1 (MQ=255)
       ggTGTATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGc                                                                                                                                  <  7:209422/149‑1 (MQ=255)
        gtgtATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGcc                                                                                                                                 <  7:27208/149‑1 (MQ=255)
         tgtATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCATCGTAGttttaaa                                                                                                                                 >  2:253868/1‑145 (MQ=255)
           tATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGccca                                                                                                                               >  2:370540/1‑147 (MQ=255)
                     cgcCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGgtc                                                                                                                     <  1:335088/148‑1 (MQ=255)
                      gcCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGgtcg                                                                                                                    >  8:125612/1‑148 (MQ=255)
                        ccGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCa                                                                                                                 <  1:370540/149‑1 (MQ=255)
                         cGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCAc                                                                                                                <  7:125612/149‑1 (MQ=255)
                          gATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCACCGTCAGCGTAGGTTAGACATCTACAAGGTCGTCAcc                                                                                                               >  6:10520/1‑149 (MQ=255)
                              cttcGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGAc                                                                                                            <  1:253868/148‑1 (MQ=255)
                               ttcGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTACCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGt                                                                                                          >  5:49705/1‑149 (MQ=255)
                                        aaCGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGcc                                                                                                   >  1:326152/1‑147 (MQ=255)
                                             gAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGACTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTc                                                                                            >  5:288602/1‑149 (MQ=255)
                                               aTCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTACCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTg                                                                                          <  6:49705/149‑1 (MQ=255)
                                                  tGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGc                                                                                         >  5:122264/1‑147 (MQ=255)
                                                   gTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGgtcgt                                                                                                                   <  3:152250/120‑1 (MQ=255)
                                                    ttCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGa                                                                                       <  7:174597/147‑1 (MQ=255)
                                                    ttCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGt                                                                                     >  3:320152/1‑149 (MQ=255)
                                                    ttCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGt                                                                                     >  7:286911/1‑149 (MQ=255)
                                                    ttCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCCGCAGTGCCTTCGCGCATGGTGTAGATCAGCGTCAGGGTAGGATAAACATCATAAAGCTCGTCACAGACGTTCTTGACCAGGCTCTGCGAga                                                                                     >  8:376916/1‑148 (MQ=255)
                                                       cAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCATGGTAGTTTTTAAATCAGACAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCAc                                                                                  >  8:184497/1‑149 (MQ=255)
                                                       cAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCa                                                                                                                 <  3:283628/118‑1 (MQ=255)
                                                       cAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCAAGGTCGTCa                                                                                                                 >  4:283628/1‑118 (MQ=255)
                                                        aGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCAcc                                                                                 >  7:7644/1‑149 (MQ=255)
                                                         gATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTTCAAACAGCAAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTc                                                                                    >  6:59752/1‑145 (MQ=255)
                                                         gATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTc                                                                                    <  5:59752/145‑1 (MQ=255)
                                                         gATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCACCGGCATCGAAGGATAAAAATAAAAAAGTTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCAccc                                                                                >  6:37668/1‑149 (MQ=255)
                                                           ttttCCCGGCCACCCTTTTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCtt                                                                              <  6:288602/147‑1 (MQ=255)
                                                               ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGGCGGCTGCTCGGCAGTGCCTTCGCGCATGGTTTAGATCAGCGGCAGGGGAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTg                                                                             <  2:290544/146‑1 (MQ=255)
                                                               ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTg                                                                             <  7:184497/146‑1 (MQ=255)
                                                               ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTg                                                                             <  4:320152/146‑1 (MQ=255)
                                                               ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTa                                                                           <  8:286911/148‑1 (MQ=255)
                                                               ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTa                                                                           <  1:258289/148‑1 (MQ=255)
                                                               ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTa                                                                           <  2:326152/148‑1 (MQ=255)
                                                               ccAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCACCGTCATGGTAGTTTTCAAATCTAAAAAGTCGTCACCGACGtt                                                                                                         >  8:35333/1‑118 (MQ=255)
                                                                cAGGCCAGCGTTCTGGATCGCCTGGCGTACCAGCGCCGCATGCTCGGCAGTGGCTTCGCGCATGGTGTAGTTCAGCGGGATGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGt                                                                         <  5:291335/149‑1 (MQ=255)
                                                                cAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCACCGTCATCGAAGTATAAAAAAAAAAAAAATCGTAACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGt                                                                         >  4:377130/1‑149 (MQ=255)
                                                                   gCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGGCTGCTCGGCTGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCca                                                                      <  6:122264/149‑1 (MQ=255)
                                                                      aCCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCcagca                                                                   <  8:7644/149‑1 (MQ=255)
                                                                       cccTTCTGGATCGCCTGGCGTACCAGCGCCTCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCTGCGGCATGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCcagcag                                                                  <  5:113145/149‑1 (MQ=255)
                                                                               gATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGa                                                          <  5:10520/149‑1 (MQ=255)
                                                                                          gTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAg                                               <  7:376916/149‑1 (MQ=255)
                                                                                          gTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAg                                               <  3:104065/149‑1 (MQ=255)
                                                                                             ccAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGcc                                            <  3:377130/149‑1 (MQ=255)
                                                                                                           tCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTc                              >  5:205443/1‑149 (MQ=255)
                                                                                                                               ggTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCtt          >  3:303502/1‑149 (MQ=255)
                                                                                                                                  gtAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAg               <  5:225222/141‑1 (MQ=255)
                                                                                                                                  gtAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAg               >  6:225222/1‑141 (MQ=255)
                                                                                                                                   tAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGGCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTcgcg      <  8:378459/149‑1 (MQ=255)
                                                                                                                                       tCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCgctgc  >  2:350702/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GCTCGGCGGTGTATTTCAGTGCGCCCGATTCTTCGACCGCAACGCGAATCTGTTCCAGATCTTCCAGGCCACCCTTCTGGATCGCCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGC  >  NC_002947/799617‑799900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑