| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 826,700 | G→C | 10.4% | intergenic (‑102/‑110) | PP_0710 ← / → ycaC‑I | hypothetical protein/putative hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 826,700 | 0 | G | C | 10.4% | 163.6 / 10.6 | 58 | intergenic (‑102/‑110) | PP_0710/ycaC‑I | hypothetical protein/putative hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (26/26); new base C (2/4); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01 | |||||||||||
CGACGAGGTCGGCGGCGGCTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAGAACAACG > NC_002947/826553‑826846 | cgacgaGGTCGGCGGCGGCTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTtgt < 5:239222/149‑1 (MQ=255) aGGTCGGCGGCGGCTTGCCGCACTTGAAGTGTCTAGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACt > 1:55193/1‑149 (MQ=255) ggTCGGCGGCGGCTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCATGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTg < 1:159792/149‑1 (MQ=255) ggTCGGCGGCGGCTTGCCGCACTTCAAGTGTCAGGACCACCGGAGTGCACTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCCAGGGCAAAAATCGCTGGCGGCAAATAATCAGTAAAGAAATTTGTCAACt > 8:155528/1‑148 (MQ=255) gTCGGCGGCGGCTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGa > 8:382673/1‑149 (MQ=255) gTCGGCGGCGGCTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCACAAATTTCTGAACTGa < 4:177850/149‑1 (MQ=255) gcggcggcTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATggg < 2:207182/149‑1 (MQ=255) gcggcggcTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATggg < 6:109213/149‑1 (MQ=255) gcggcTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCACAAATTTCTGAACTGAttatttg < 6:383906/149‑7 (MQ=255) cggcTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGcac < 7:357506/149‑1 (MQ=255) ttGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAggg > 7:212395/1‑149 (MQ=255) gCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAggg < 7:382673/147‑1 (MQ=255) aCTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTa > 8:78396/1‑147 (MQ=255) aCTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGGGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATGTGTGAACTGATGGGGCACAGGGCAGTACa < 6:227520/149‑1 (MQ=255) gAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTtgt < 8:376738/119‑1 (MQ=255) gAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTtgt > 7:376738/1‑119 (MQ=255) gAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATg > 6:368302/1‑128 (MQ=255) gAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATg < 5:368302/128‑1 (MQ=255) tCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGcac > 5:113957/1‑128 (MQ=255) tCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGcac < 6:113957/128‑1 (MQ=255) tCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAgg > 8:63011/1‑131 (MQ=255) gtgcACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGAc < 7:155528/143‑1 (MQ=255) gTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGATAAATCGCTGGCGGCAAATAATCAGTTCAGAAAATTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGa > 2:82445/1‑149 (MQ=255) gTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGa < 3:198957/149‑1 (MQ=255) cACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACgg > 8:98703/1‑149 (MQ=255) aCGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGAc > 7:253826/1‑142 (MQ=255) aCGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGAc < 8:253826/142‑1 (MQ=255) gggAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGtct < 8:212395/149‑1 (MQ=255) aCGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTc < 2:55193/148‑1 (MQ=255) gTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATc > 7:73634/1‑149 (MQ=255) gTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATc < 7:98703/149‑1 (MQ=255) ttGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCACAAATTTCTGAACTGAt > 4:98769/1‑90 (MQ=255) ttGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCACAAATTTCTGAACTGAt < 3:98769/90‑1 (MQ=255) ttGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCt > 5:324057/1‑149 (MQ=255) tggtgAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCt < 6:148733/146‑1 (MQ=255) tggtgAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCt > 5:148733/1‑146 (MQ=255) aTTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAAcc < 7:78396/149‑1 (MQ=255) ggCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAg > 2:288819/1‑149 (MQ=255) gTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCTACCCGCTGAAGCAAg < 6:324057/149‑1 (MQ=255) gTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAg < 1:288819/149‑1 (MQ=255) ggCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAg > 3:204875/1‑146 (MQ=255) aaaaaTCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACtgt > 2:294016/1‑149 (MQ=255) aaaaTCGCTGGCGGCAAATAATCAGTTCAGAAAATTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACtgtg < 1:82445/149‑1 (MQ=255) gCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTc < 1:294016/149‑1 (MQ=255) cTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTcc < 4:204875/149‑1 (MQ=255) ggcggcAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCaa > 7:299672/1‑149 (MQ=255) gcggcAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCaa > 3:195403/1‑148 (MQ=255) ggcAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGc < 2:27506/59‑1 (MQ=255) ggcAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGc > 1:27506/1‑59 (MQ=255) ggcAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATa > 8:149786/1‑148 (MQ=255) aataatCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGc < 3:374163/149‑1 (MQ=255) ataatCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTc > 6:26412/1‑101 (MQ=255) ataatCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTc < 5:26412/101‑1 (MQ=255) ccatCAGTTCACAAATTTCTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGt > 1:135286/3‑149 (MQ=255) cacaaaTTTCTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACaa < 2:135286/145‑1 (MQ=255) aGAAATTTGTTAACTGATGGTGCACAGGGCAGTACAGGCAAGCCTACTGAAGTGCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCACCCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAGa < 8:369750/149‑1 (MQ=255) aGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAg > 5:357269/1‑148 (MQ=255) gAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAGa > 8:377460/1‑148 (MQ=255) aaaTTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAGaa > 8:18056/1‑148 (MQ=255) aaaTTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAGaa > 4:273800/1‑148 (MQ=255) tttGTGAACTTATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAGaacaac < 7:149786/149‑1 (MQ=255) tgtgAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTAAGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAGAACAACg < 7:327341/148‑1 (MQ=255) | CGACGAGGTCGGCGGCGGCTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAGAACAACG > NC_002947/826553‑826846 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |