Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	907,475	G→A	5.5%	Q246* (CAG→TAG) ‡	yjjV ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	907,475	0	G	A	5.5%	284.3 / 3.9	90	Q246* (CAG→TAG) ‡	yjjV	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base G (46/39);  new base A (3/2);  total (49/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

GTTGCGCTGTGCAGTGCTGTGGTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAGCACCACACTTTCCAGT  >  NC_002947/907332‑907617                                                                                                                                                |                                                                                                                                               gTTGCGCTGTGCAGTGCTGTGGTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGc                                                                                                                                             >  7:43018/1‑147 (MQ=255)    gcgcTGTGCAGTGCTGTGGTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAg                                                                                                                                          <  4:213952/147‑1 (MQ=255)    gcgcTGTGCAGTGCTGTGGTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAg                                                                                                                                          >  3:213952/1‑147 (MQ=255)      gcTGTGCAGTGCTGTGGTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCAcc                                                                                                                                      >  2:277250/1‑149 (MQ=255)          tgCAGTGCTGTGGTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTc                                                                                                                                    <  3:278078/147‑1 (MQ=255)            cAGTGCTGTGGTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGcc                                                                                                                                            <  5:95700/137‑1 (MQ=255)                  tgtgGTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGc                                                                                                                          >  2:395632/1‑149 (MQ=255)                  tgtgGTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGc                                                                                                                          <  3:338924/149‑1 (MQ=255)                     ggTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGccc                                                                                                                        >  4:57763/1‑148 (MQ=255)                         tGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCa                                                                                                                   >  3:298341/1‑149 (MQ=255)                            cGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGt                                                                                                                <  3:57763/149‑1 (MQ=255)                                   gcgcTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAg                                                                                                         >  1:367875/1‑149 (MQ=255)                                    cgcTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAgc                                                                                                        >  7:343768/1‑149 (MQ=255)                                         tgGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGcc                                                                                                     <  2:367875/147‑1 (MQ=255)                                           gACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTgg                                                                                                 >  5:176788/1‑149 (MQ=255)                                              cGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCaa                                                                                              <  6:86143/149‑1 (MQ=255)                                              cGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCaa                                                                                              >  8:208994/1‑149 (MQ=255)                                               gTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAAt                                                                                             >  6:189900/1‑149 (MQ=255)                                               gTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAAt                                                                                             >  2:299011/1‑149 (MQ=255)                                                     gTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTcc                                                                                         <  3:385944/147‑1 (MQ=255)                                                     gTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCgg                                                                                       <  2:317747/149‑1 (MQ=255)                                                     gTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCgg                                                                                       >  5:90073/1‑149 (MQ=255)                                                      tCTGATTCGTGGCCAATCTCATCCCACGCGAATCCCGGTCACCCTAGCTTCACCAGCCTAACAACGTGCAAGATTTGGGTGTGCTGGCCTGTGCCAGCACCTCGACATCCAGcc                                                                                                                         <  8:30601/114‑1 (MQ=255)                                                      tCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGcc                                                                                                                         >  7:30601/1‑114 (MQ=255)                                                          aTTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCTCAAGCTCCGTGGCAATTTCCGGCAGAt                                                                                  >  7:55619/1‑149 (MQ=255)                                                          aTTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGAt                                                                                  >  2:39762/1‑149 (MQ=255)                                                              ggggCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCaa                                                                                              <  7:113982/133‑1 (MQ=255)                                                              ggggCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCaa                                                                                              >  8:113982/1‑133 (MQ=255)                                                              ggggCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTc                                                                              >  2:72585/1‑149 (MQ=255)                                                                  ccAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCggg                                                                          <  1:299011/149‑1 (MQ=255)                                                                  ccAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCggg                                                                          <  1:277250/149‑1 (MQ=255)                                                                      tCGCATCCCACGCGAATCCCGGTAACCCGAGCTGCACCAGCCGAACAACGTGCAAGAATTGCGGGTGCTGGCCTGTGCCAGCAACTCGACATCCAGCCCCATCACGTGCGCAAGCGCAGTGGCAAGTTCCGTCAGCTTCTCCGGGCTGt                                                                      >  2:123292/1‑149 (MQ=255)                                                                        gCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGtt                                                                     <  1:39762/148‑1 (MQ=255)                                                                             ccACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATc                                                                                                                              >  7:234167/1‑86 (MQ=255)                                                                             ccACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATc                                                                                                                              <  8:234167/86‑1 (MQ=255)                                                                              cACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCC‑GGCTGTTGCGTAccc                                                             >  4:176615/1‑149 (MQ=255)                                                                                      tCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTa                                                      >  3:90690/1‑149 (MQ=255)                                                                                      tCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTa                                                      >  2:316191/1‑149 (MQ=255)                                                                                       cccGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTa                                                      >  6:274256/1‑148 (MQ=255)                                                                                         cGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCgg                                                         >  3:68085/1‑143 (MQ=255)                                                                                         cGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCgg                                                         <  4:68085/143‑1 (MQ=255)                                                                                          ggTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATc                                                  <  6:90073/149‑1 (MQ=255)                                                                                          ggTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATc                                                  >  6:274370/1‑149 (MQ=255)                                                                                              aCCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCgg                                                         >  6:118999/1‑138 (MQ=255)                                                                                              aCCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCgg                                                         <  5:118999/138‑1 (MQ=255)                                                                                              aCCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCg                                              >  4:1844/1‑149 (MQ=255)                                                                                                ccGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCggg                                                                          >  4:339311/1‑119 (MQ=255)                                                                                                ccGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCggg                                                                          <  3:339311/119‑1 (MQ=255)                                                                                                ccGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGt                                            <  3:1844/149‑1 (MQ=255)                                                                                                 cGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTcc                                                                             >  8:105931/1‑115 (MQ=255)                                                                                                 cGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTcc                                                                             <  7:105931/115‑1 (MQ=255)                                                                                                 cGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTg                                           >  7:318717/1‑149 (MQ=255)                                                                                                 cGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTg                                           <  5:274370/149‑1 (MQ=255)                                                                                                  gAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGc                                                                                                <  5:243605/95‑1 (MQ=255)                                                                                                  gAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGc                                                                                                >  6:243605/1‑95 (MQ=255)                                                                                                  gAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGc                                          <  4:90690/149‑1 (MQ=255)                                                                                                  gAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGc                                          <  5:189900/149‑1 (MQ=255)                                                                                                   aGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGcc                                         <  8:343768/149‑1 (MQ=255)                                                                                                        cACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCTGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTc                                    >  6:167280/1‑149 (MQ=255)                                                                                                         aCCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTcc                                   >  7:156824/1‑149 (MQ=255)                                                                                                          ccAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCg                                  <  7:208994/149‑1 (MQ=255)                                                                                                           cAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGt                                                       <  4:23273/127‑1 (MQ=255)                                                                                                           cAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGt                                                       >  3:23273/1‑127 (MQ=255)                                                                                                           cAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCgg                                 >  6:134257/1‑149 (MQ=255)                                                                                                             gCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAgcg                                                                                                       <  2:295494/77‑1 (MQ=255)                                                                                                             gCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAgcg                                                                                                       >  1:295494/1‑77 (MQ=255)                                                                                                               cGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGag                             >  1:299533/1‑149 (MQ=255)                                                                                                                  acaacGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGagtca                          <  5:134257/149‑1 (MQ=255)                                                                                                                       gTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGtctc                     <  1:395632/149‑1 (MQ=255)                                                                                                                        tGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTcc                    <  1:316191/149‑1 (MQ=255)                                                                                                                          cAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAg                  <  5:274256/149‑1 (MQ=255)                                                                                                                           aaGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTcc                                                                                         <  1:19149/77‑1 (MQ=255)                                                                                                                           aaGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTcc                                                                                         >  2:19149/1‑77 (MQ=255)                                                                                                                           aaGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAg                  >  5:254251/1‑148 (MQ=255)                                                                                                                            aGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCACCCCCATCACGTGCGGAAGCGCCGTGCCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCGCCGGCTACATCGCCGGTGACATGTACGGAGAGTCAGACTCCAGca                >  1:382939/1‑149 (MQ=255)                                                                                                                               aTTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCg                                  >  3:80965/1‑128 (MQ=255)                                                                                                                               aTTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCg                                  <  4:80965/128‑1 (MQ=255)                                                                                                                                  cgaggTGCTGGCACAGGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGtctc                     <  3:56127/135‑1 (MQ=255)                                                                                                                                  cgaggTGCTGGCACAGGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTACGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGtctc                     >  4:56127/4‑138 (MQ=255)                                                                                                                                   cGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAGCACCACACt         >  6:98472/1‑149 (MQ=255)                                                                                                                                    aggTGCTGGCACAGGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCggg                                                        >  8:5074/2‑101 (MQ=255)                                                                                                                                    aggTGCTGGCACAGGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCggg                                                        <  7:5074/100‑1 (MQ=255)                                                                                                                                     ggTGCTGGCACAGGCCAGCACCTCGACACCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAGCACCACACttt       >  8:330173/1‑149 (MQ=255)                                                                                                                                         cTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCggg                                                        >  2:326857/1‑96 (MQ=255)                                                                                                                                         cTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCggg                                                        <  1:326857/96‑1 (MQ=255)                                                                                                                                         cTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAGCACCACACTTTCCAg   >  7:237737/1‑149 (MQ=255)                                                                                                                                         cTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAGCACCACACTTTCCAg   <  6:254251/149‑1 (MQ=255)                                                                                                                                          tGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAGCACCACACTTTCCAGt  >  4:215984/1‑149 (MQ=255)                                                                                                                                            gCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAGCACCACACTTTCCAGt  <  8:318717/147‑1 (MQ=255)                                                                                                                                            gCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAGCACCACACTTTCCAGt  <  1:235442/147‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               GTTGCGCTGTGCAGTGCTGTGGTATGCCGGGTTCGCGCTGTGGACCGTGCTGGTCTGATTCGGGGCCAATCGCATCCCACGCGAATCCCGGTCACCCGAGCTTCACCAGCCGAACAACGTGCAAGCATTGCGGGTGCTGGCCTGTGCCAGCACCTCGACATCCAGCCCCATCACGTGCGCAAGCGCCGTGGCAATTTCCGGCAGATGCTCCGGGCTGTTGCGTACCCCCGGGTACATCGCCGGTGCCATGTCCGGTGAGTCAGTCTCCAGCACCACACTTTCCAGT  >  NC_002947/907332‑907617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑