Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 953,229 A→G 11.8% H13R (CAC→CGC) ‡ cyoC → cytochrome bo terminal oxidase subunit III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947953,2290AG11.8% 128.9 / 8.5 52H13R (CAC→CGC) ‡cyoCcytochrome bo terminal oxidase subunit III
Reads supporting (aligned to +/- strand):  ref base A (23/22);  new base G (2/4);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01

CGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCGGCCCGTCG  >  NC_002947/953089‑953377
                                                                                                                                            |                                                                                                                                                    
cGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCTCGGCGCTGCTCATGGTCACGACCATg                                                                                                                                              <  2:289042/149‑1 (MQ=255)
 gaagaaGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATgg                                                                                                                                             <  6:208287/149‑1 (MQ=255)
 gaagaaGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATga                                                                                                                                             <  3:321806/149‑2 (MQ=255)
   agaagGGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTTCTCTGCCTGCCGGCGCACGTGTCGAATCTTTTTATCGGGTTTAATCAATGTCCAGTCAAGTATTGCACGTCGCTGCCCATGGTCGTGACCATGAGc                                                                                                                                           <  6:414410/149‑1 (MQ=255)
          gcgcgcATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGTCGCACGTGTCGAATCTTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGTGCATgacg                                                                                                                                     <  1:126141/148‑1 (MQ=255)
            gcgcATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATgactac                                                                                                                                   >  7:145441/1‑148 (MQ=255)
                         gAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGaccac                                                                                                                                >  8:309824/1‑138 (MQ=255)
                         gAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGaccac                                                                                                                                <  7:309824/138‑1 (MQ=255)
                         gAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTc                                                                                                                     >  1:16383/1‑149 (MQ=255)
                           gCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCgg                                                                                                                   >  3:12437/1‑149 (MQ=255)
                              tATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGcc                                                                                                                <  3:145334/149‑1 (MQ=255)
                              tATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGc                                                                                                                 >  6:242957/1‑148 (MQ=255)
                                 gAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGAGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGc                                                                                                                 >  8:44132/1‑145 (MQ=255)
                                   aaGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATg                                                                                                           <  7:348300/149‑1 (MQ=255)
                                       gcTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCg                                                                                                       <  4:12437/149‑1 (MQ=255)
                                             cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTgg                                                                                                 >  4:250161/1‑149 (MQ=255)
                                               aaGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGc                                                                                               <  5:220847/149‑1 (MQ=255)
                                                  cAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTc                                                                                            <  7:238558/149‑1 (MQ=255)
                                                       gCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCt                                                                                       <  7:335029/149‑1 (MQ=255)
                                                        ctctGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTg                                                                                      >  7:144305/1‑149 (MQ=255)
                                                         tctGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTg                                                                                      >  4:48025/1‑148 (MQ=255)
                                                           tgcctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGt                                                                                     <  2:16383/147‑1 (MQ=255)
                                                             cctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTAc                                                                                   <  5:151040/147‑1 (MQ=255)
                                                             cctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCt                                                                                 >  6:73491/1‑149 (MQ=255)
                                                             cctgccGGCGCACGTGTCGAATCGTTTGAGCGAGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTAcc                                                                                  <  7:44132/148‑1 (MQ=255)
                                                              ctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTggg                                                                                                >  8:155270/1‑133 (MQ=255)
                                                              ctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTgg                                                                                         >  1:166581/1‑140 (MQ=255)
                                                              ctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTgg                                                                                         <  2:166581/140‑1 (MQ=255)
                                                                   ggCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGa                                                                                                          >  2:288165/1‑118 (MQ=255)
                                                                   ggCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGa                                                                                                          <  1:288165/118‑1 (MQ=255)
                                                                      gcACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGa                                                                        >  4:150463/1‑149 (MQ=255)
                                                                            gtCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCAt                                                                  >  1:301064/1‑149 (MQ=255)
                                                                               gAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCt                                                               >  4:377277/1‑149 (MQ=255)
                                                                                          gCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGc                                                    >  4:381623/1‑149 (MQ=255)
                                                                                                      aaTGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCt                                        <  3:48025/149‑1 (MQ=255)
                                                                                                         gTCCAGTCAAGTAATGCACGGCGCTGCTTATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTAcg                                     >  3:357789/1‑149 (MQ=255)
                                                                                                            cAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCgg                                  <  8:144305/149‑1 (MQ=255)
                                                                                                              gTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCATCGCTCTTCGCCACCTACGCGGTg                                <  3:250161/149‑1 (MQ=255)
                                                                                                                 aaGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCt                              <  3:377277/148‑1 (MQ=255)
                                                                                                                 aaGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTg                             >  3:76394/1‑149 (MQ=255)
                                                                                                                  aGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTc                                               >  4:122858/1‑130 (MQ=255)
                                                                                                                  aGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTc                                               <  3:122858/130‑1 (MQ=255)
                                                                                                                  aGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGt                            >  5:348211/1‑149 (MQ=255)
                                                                                                                         cACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAg                     <  5:242957/149‑1 (MQ=255)
                                                                                                                                 tgctCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGtttt                 <  4:352143/145‑1 (MQ=255)
                                                                                                                                 tgctCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGtttt                 >  3:352143/1‑145 (MQ=255)
                                                                                                                                  gcccATGGTCGTGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGc                                                         >  1:125414/4‑104 (MQ=255)
                                                                                                                                  gcccATGGTCGTGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGc                                                         <  2:125414/101‑1 (MQ=255)
                                                                                                                                  gcccATGGTCGTGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTg                                <  4:152424/126‑1 (MQ=255)
                                                                                                                                  gcccATGGTCGTGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTg                                >  3:152424/4‑129 (MQ=255)
                                                                                                                                      aTGGTCCCGACCATGGGCATGACGCCCACCACCCCACCTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCggcggc        >  6:330591/1‑149 (MQ=255)
                                                                                                                                          tCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCGGCCCGt    >  5:227765/1‑149 (MQ=255)
                                                                                                                                            aCGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCGGCCCGTCg  >  2:378058/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
CGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCGGCCCGTCG  >  NC_002947/953089‑953377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑