Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	990,589	G→A	6.7%	R157H (CGC→CAC) ‡	PP_0856 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	990,589	0	G	A	6.7%	220.9 / 4.2	74	R157H (CGC→CAC) ‡	PP_0856	lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (36/33);  new base A (2/3);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

GTCATGCTCGGTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGTG  >  NC_002947/990444‑990737                                                                                                                                                  |                                                                                                                                                     gTCATGCTCGGTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGc                                                                                                                                                     <  8:29239/147‑1 (MQ=255) gTCATGCTCGGTACCCTCAAGGGTGAAGGCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCt                                                                                                                                                   <  5:169292/149‑1 (MQ=255)      gCTCGGTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCg                                                                                                                                              >  8:216231/1‑149 (MQ=255)        tCGGTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGc                                                                                                                                            >  6:263715/1‑149 (MQ=255)          ggTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCt                                                                                                                                          <  6:386024/149‑1 (MQ=255)            tACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                        <  3:316889/149‑1 (MQ=255)                   aaGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATgccgc                                                                                                                                 >  4:297519/1‑149 (MQ=255)                        tGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                        <  1:266708/137‑1 (MQ=255)                        tGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                        >  2:266708/1‑137 (MQ=255)                          aaGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGc                                                                                                                          <  7:216231/149‑1 (MQ=255)                             tCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGAc                                                                                                                       <  2:9025/149‑1 (MQ=255)                               aTTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                        >  3:389131/1‑130 (MQ=255)                               aTTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                        <  4:389131/130‑1 (MQ=255)                               aTTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGAccg                                                                                                                     >  7:153074/1‑149 (MQ=255)                                   cccTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGCCCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGt                                                                                                                 >  7:355600/1‑149 (MQ=255)                                        gACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTgg                                                                                                              <  3:297519/147‑1 (MQ=255)                                             cAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTAc                                                                                                        >  5:415219/1‑148 (MQ=255)                                              aGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGAt                                                                                                                                      >  5:135024/1‑117 (MQ=255)                                              aGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGAt                                                                                                                                      <  6:135024/117‑1 (MQ=255)                                              aGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACg                                                                                                       >  2:299564/1‑148 (MQ=255)                                                   cGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACt                                                                                                 >  3:159227/1‑149 (MQ=255)                                                   cGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACt                                                                                                 >  5:7627/1‑149 (MQ=255)                                                    ggTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTc                                                                                                <  7:91317/149‑1 (MQ=255)                                                     gTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCg                                                                                               >  7:390538/1‑149 (MQ=255)                                                          gagCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGAGCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggc                                                                                          >  3:138966/1‑149 (MQ=255)                                                          gagCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggc                                                                                          <  4:159227/149‑1 (MQ=255)                                                          gagCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggc                                                                                          >  3:13403/1‑149 (MQ=255)                                                           agCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggcc                                                                                         <  4:69074/149‑1 (MQ=255)                                                           agCGGTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggcc                                                                                         <  4:13403/149‑1 (MQ=255)                                                                 ggCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCt                                                                                    <  7:33694/148‑1 (MQ=255)                                                                  gcgcTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGa                                                                                  >  3:27167/1‑149 (MQ=255)                                                                      tCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCt                                                                              >  2:231427/1‑149 (MQ=255)                                                                        gcgcgTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGc                                                                            <  6:415219/149‑1 (MQ=255)                                                                        gcgcgTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGc                                                                            >  7:146122/1‑149 (MQ=255)                                                                          gtgTGACCATCGAAGTGCTGGCCCCTCCTGCCAACAACGGTGAGGTGGTGGTGGTGCAGAGCCAGGACTATCGCCTGATGGGCGTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGt                                                                          <  4:138966/147‑1 (MQ=255)                                                                           cgTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTg                                                                         <  1:299564/149‑1 (MQ=255)                                                                            gTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTgg                                                                        <  2:132115/149‑1 (MQ=255)                                                                                ccAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGGTGGATCTAc                                                                                                        >  7:339888/1‑113 (MQ=255)                                                                                 cAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCg                                                                   >  8:168086/1‑149 (MQ=255)                                                                                  aGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgc                                                                                              >  8:120180/1‑121 (MQ=255)                                                                                  aGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgc                                                                                              <  7:120180/121‑1 (MQ=255)                                                                                      aaGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGtt                                                                                                                <  5:411597/99‑1 (MQ=255)                                                                                      aaGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGtt                                                                                                                >  6:411597/1‑99 (MQ=255)                                                                                         tGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGcc                                                            >  3:157180/1‑148 (MQ=255)                                                                                              gCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATc                                                        <  7:168086/147‑1 (MQ=255)                                                                                               ccccACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGc                                                      >  4:401702/1‑148 (MQ=255)                                                                                                cccACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGcca                                                    <  5:263715/149‑1 (MQ=255)                                                                                                         caacaaCGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGcc                                           >  3:355264/1‑149 (MQ=255)                                                                                                         caacaaCGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGcc                                           >  6:358653/1‑149 (MQ=255)                                                                                                          aacaacGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGTTGCCGCCTCTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCg                                                                   >  8:138443/1‑124 (MQ=255)                                                                                                          aacaacGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGTTGCCGCCTCTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCg                                                                   <  7:138443/124‑1 (MQ=255)                                                                                                            caacGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCTCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTgg                                        <  8:153074/149‑1 (MQ=255)                                                                                                             aacGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGc                                       >  3:373922/1‑149 (MQ=255)                                                                                                                    aCGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGc                                 <  8:284226/148‑1 (MQ=255)                                                                                                                    aCGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGc                                 >  7:284226/1‑148 (MQ=255)                                                                                                                     cGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCg                               <  8:146122/149‑1 (MQ=255)                                                                                                                      gtggtggtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTg                                         <  4:243116/138‑1 (MQ=255)                                                                                                                      gtggtggtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTg                                         >  3:243116/1‑138 (MQ=255)                                                                                                                         gtggtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCt                           <  4:27167/149‑1 (MQ=255)                                                                                                                            gtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCa                                                                      <  7:125112/103‑1 (MQ=255)                                                                                                                            gtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCa                                                                      >  8:125112/1‑103 (MQ=255)                                                                                                                               gtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTAc                     <  4:373922/149‑1 (MQ=255)                                                                                                                                     aCCCAGGCCGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAATCTCCCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCaa               <  8:355600/149‑1 (MQ=255)                                                                                                                                       ccAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCt                           >  3:336124/1‑135 (MQ=255)                                                                                                                                       ccAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCt                           <  4:336124/135‑1 (MQ=255)                                                                                                                                         aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGGTCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAgg             >  4:373196/1‑147 (MQ=255)                                                                                                                                         aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAgg             >  4:378171/1‑147 (MQ=255)                                                                                                                                         aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCAGGCGGTTggtg                               >  4:76799/1‑126 (MQ=255)                                                                                                                                         aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCAGGCGGTTggtg                               <  3:76799/129‑4 (MQ=255)                                                                                                                                           gACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGt         >  8:46203/1‑149 (MQ=255)                                                                                                                                              gATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGc      >  3:388125/1‑149 (MQ=255)                                                                                                                                              gATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGc      >  8:284371/1‑149 (MQ=255)                                                                                                                                               aTCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCg     <  4:355264/149‑1 (MQ=255)                                                                                                                                                tCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCgg    <  3:401702/149‑1 (MQ=255)                                                                                                                                                  gCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGt   <  7:284371/148‑1 (MQ=255)                                                                                                                                                  gCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGTg  <  6:7627/149‑1 (MQ=255)                                                                                                                                                  gCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGTg  <  4:157180/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                     GTCATGCTCGGTACCCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGCGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGTG  >  NC_002947/990444‑990737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑